A study in vitro on differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in mice
Abstract
Objective To investigate the differentiation conditions of bone marrow mesenchymal stem cells (BMSCs) into endometrial epithelial cells and to confirm the effect of 17β-estradiol in this process.Study design BMSCs were cultured alone or co-cultured with endometrial stromal cells (EStCs) in control/differentiation medium (17β-estradiol, growth factors) and were co-cultured with EStCs in different concentrations of 17β-estradiol. Flow cytometry and immunocytochemistry were used to identify the isolated cells. Real-time RT-PCR and immunofluorescence were used to test the expression of epithelial cell markers.Results The epithelial markers cytokeratin-7, cytokeratin-18, cytokeratin-19, and epithelial membrane antigen were elevated in real-time RT-PCR (P<0.05), and cytokeratin was strongly positive in immunofluorescence analysis in the differentiated BMSCs. Cytokeratin-7 and cytokeratin-19 expression levels were highest in the 1 × 10⁻⁸ mol/L 17β-estradiol group, as shown in real-time RT-PCR (P<0.05).Conclusion BMSCs could be differentiated in the direction of endometrial epithelial cells in appropriate conditions in vitro: 17β-estradiol may play a key role in stimulating BMSCs' epithelial differentiation in the process of endometriosis.Condensation Bone marrow mesenchymal stem cells can differentiate in the direction of endometrial epithelial cells in a certain microenvironment and appropriate concentration of 17β-E₂ can facilitate this differentiation.
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Some mesenchymal/mesothelial cells of the peritoneal cavity, of mesothelial repair after surgery, and in endometrium and endometriosis are directly derived from bone marrow.
“Some of these mesenchymal/ mesothelial cells of the peritoneal cavity (48), of the mesothelial repair after surgery (126) and in endometrium and endometriosis (127-131) are directly derived from bone marrow.”
From Pathogenesis of deep endometriosis (2017) · cites this paper as [129]
Cites
Analyzing real-time PCR data by the comparative CT method
Schmittgen, T. D., & Livak, K. J. (2008) · Nature Protocols · doi:10.1038/nprot.2008.73 · Cited by 26,190 · see citing works
Sampson, J. A. (1927) · American Journal of Obstetrics and Gynecology · doi:10.1016/s0002-9378(15)30003-x · Cited by 2,334 · see citing works
Estrogen Receptor Null Mice: What Have We Learned and Where Will They Lead Us?
Couse, J. F., & Korach, K. S. (1999) · Endocrine Reviews · doi:10.1210/edrv.20.3.0370 · Cited by 2,045 · see citing works
Eskenazi, B., & Warner, M. L. (1997) · Obstetrics and Gynecology Clinics of North America · doi:10.1016/s0889-8545(05)70302-8 · Cited by 1,617 · see citing works
Clonogenicity of Human Endometrial Epithelial and Stromal Cells1
Chan, R. W. S., Schwab, K. E., & Gargett, C. E. (2004) · Biology of Reproduction · doi:10.1095/biolreprod.103.024109 · Cited by 653 · see citing works
Stromal estrogen receptors mediate mitogenic effects of estradiol on uterine epithelium
Cooke, P. S., Buchanan, D. L., Young, P., Setiawan, T., Brody, J. R., Korach, K. S., Taylor, J., Lubahn, D. B., & Cunha, G. R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.12.6535 · Cited by 556 · see citing works
Estrogen Receptor Null Mice: What Have We Learned and Where Will They Lead Us?
Couse, J. F. (1999) · Endocrine Reviews · doi:10.1210/er.20.3.358 · Cited by 532 · see citing works
Endometrial cells derived from donor stem cells in bone marrow transplant recipients
Taylor, H. S. (2004) · JAMA · doi:10.1001/jama.292.1.81 · Cited by 510 · see citing works
Contribution of Bone Marrow-Derived Stem Cells to Endometrium and Endometriosis
Du, H., & Taylor, H. S. (2007) · Stem Cells · doi:10.1634/stemcells.2006-0828 · Cited by 449 · cited 3× · see citing works
“Furthermore, studies from Taylor’s group showed that non-uterine stem cells, such as bone marrow stem cells, can also contribute to the regeneration of endometrial tissue, as endometrial cells originating from donor-derived bone marrow cells were found in bone marrow transplant recipients both in humans [14] and in mi…”
Background<i>Stem Cells and the Pathogenesis of Endometriosis</i>
Sasson, I. E., & Taylor, H. S. (2008) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1434.014 · Cited by 371 · see citing works
Endometriosis: The pathophysiology as an estrogen-dependent disease
Kitawaki, J., Kado, N., Ishihara, H., Koshiba, H., Kitaoka, Y., & Honjo, H. (2002) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/s0960-0760(02)00260-1 · Cited by 370 · see citing works
Endometriosis results from the dislocation of basal endometrium
Leyendecker, G. (2002) · Human Reproduction · doi:10.1093/humrep/17.10.2725 · Cited by 300 · see citing works
Identification of Label-Retaining Cells in Mouse Endometrium
Chan, R., & Gargett, C. E. (2006) · Stem Cells · doi:10.1634/stemcells.2005-0411 · Cited by 265 · see citing works
Differential Response of Individual Uterine Cell Types from Immature Rats Treated with Estradiol*
McCormack, S. A., & Glasser, S. R. (1980) · Endocrinology · doi:10.1210/endo-106-5-1634 · Cited by 209 · see citing works
Hormone and growth factor signaling in endometrial renewal: Role of stem/progenitor cells
Gargett, C. E., Chan, R., & Schwab, K. E. (2008) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2008.02.026 · Cited by 192 · see citing works
Regeneration in the Primate Uterus: The Role of Stem Cells
Padykula, H. A. (1991) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1991.tb37849.x · Cited by 183 · see citing works
Estrogen biosynthesis in endometriosis: molecular basis and clinical relevance
Bulun, S. E., Zeitoun, K. M., Takayama, K., & Sasano, H. (2000) · Journal of Molecular Endocrinology · doi:10.1677/jme.0.0250035 · Cited by 170 · see citing works
Isolation and Characterization of Mouse Mesenchymal Stem Cells
Sung, J. H., Yang, H.-I., Park, J. B., Choi, G. S., Joh, J., Kwon, C. H. D., Chun, J. M., Lee, S.-K., & Kim, S.-J. (2008) · Transplantation Proceedings · doi:10.1016/j.transproceed.2008.08.009 · Cited by 166 · see citing works
Current Concepts in the Pathogenesis of Endometriosis
Witz, C. A. (1999) · Clinical Obstetrics & Gynecology · doi:10.1097/00003081-199909000-00013 · Cited by 151 · see citing works
Current concepts of the pathogenesis of endometriosis
Osuga, Y. (2009) · Reproductive Medicine and Biology · doi:10.1007/s12522-009-0031-z · Cited by 147 · see citing works
THE PATHOGENESIS OF ENDOMETRIOSIS
Levander, G., & NORMANN, P. (1955) · Acta Obstetricia Et Gynecologica Scandinavica · doi:10.3109/00016345509158287 · Cited by 119 · see citing works
Wada‐Hiraike, O., Hiraike, H., Okinaga, H., Imamov, O., Barros, R. P. A., Morani, A., Omoto, Y., Warner, M. N., & Gustafsson, J.-Α. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0608861103 · Cited by 118 · see citing works
Identification and characterisation of human endometrial stem/progenitor cells
Gargett, C. E. (2006) · Australian and New Zealand Journal of Obstetrics and Gynaecology · doi:10.1111/j.1479-828x.2006.00582.x · Cited by 116 · see citing works
Implantation versus infiltration: the Sampson versus the endometriotic disease theory
Koninckx, P. R., Barlow, D., & Kennedy, S. (1999) · Gynecologic and Obstetric Investigation · doi:10.1159/000052853 · Cited by 100 · see citing works
Gargett, C. E. (2004) · Australian and New Zealand Journal of Obstetrics and Gynaecology · doi:10.1111/j.1479-828x.2004.00290.x · Cited by 87 · see citing works
Jd, M., & Ae, H. (1985) · PubMed · Cited by 84 · see citing works
Thibodeau, L. L., Prioleau, G. R., Manuelidis, E. E., Merino, M. J., & Heafner, M. D. (1987) · Journal of neurosurgery · doi:10.3171/jns.1987.66.4.0609 · Cited by 83 · see citing works
(1989) · PubMed · Cited by 77 · see citing works
Kurita, T., Medina, R. T., Schabel, A. B., Young, P., Gama, P., Parekh, T. V., Brody, J. R., Cunha, G. R., Osteen, K. G., Bruner‐Tran, K. L., et al. (2005) · Differentiation · doi:10.1111/j.1432-0436.2005.00033.x · Cited by 67 · see citing works
A clinical and pathologic study of endometriosis of the lung.
Lattes, R., Shepard, F. M., Tovell, H. M. M., & Wylie, R. H. (1956) · PubMed · Cited by 62 · see citing works
Lu, X.-Z., Jim-hong, W. A. N. G., Wu, X., et al. (2008) · Acta Pharmacologica Sinica · doi:10.1111/j.1745-7254.2008.00874.x · Cited by 38 · see citing works
Peng, C., Ma, X.-C., Xue, Y., et al. (2003) · PubMed · Cited by 38 · see citing works
Simvastatin induces estrogen receptor-alpha (ER-α) in murine bone marrow stromal cells
Song, C., Wang, J., Song, Q., Li, X., Chen, Z., Ma, Q., Liu, Z., Jia, H., & Dang, G. (2008) · Journal of Bone and Mineral Metabolism · doi:10.1007/s00774-007-0820-6 · Cited by 28 · see citing works
Development of endometriosis from embryonic duct remnants
Mai, K. T., Yazdi, H. M., Perkins, D. G., & Parks, W. R. (1998) · Human Pathology · doi:10.1016/s0046-8177(98)90110-1 · Cited by 24 · see citing works
程鹏, 马绪臣, 薛延, et al. (2003) · 中华医学杂志：英文版 · Cited by 19 · see citing works
Lane, F. L., & Jacobs, S. (2012) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2012.01.045 · Cited by 18 · see citing works
Flagged first, then by citations
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Pathogenesis of endometriosis: The genetic/epigenetic theory
Koninckx, P. R., Ussia, A., Adamyan, L., Wattiez, A., Gomel, V., & Martin, D. C. (2019) · Fertility and Sterility · doi:10.1016/j.fertnstert.2018.10.013 · Cited by 427 · see citing works
Pathogenesis of deep endometriosis
Gordts, S., Koninckx, P., & Brosens, I. (2017) · Fertility and Sterility · doi:10.1016/j.fertnstert.2017.08.036 · Cited by 241 · see citing works
- Authors
- Zhang, W.-B., Cheng, M.-J., Huang, Y.-T., Jiang, W., Cong, Q., Zheng, Y.-F., & Xu, C.-J.
- Affiliations
- Shanghai Medical College of Fudan University (CN)
- Venue
- European Journal of Obstetrics & Gynecology and Reproductive Biology
- Publisher
- Elsevier BV
- Published
- 2012-02
- Volume
- 160
- Issue
- 2
- Pages
- 185-190
- ISSN
- 0028-2243 (ISSN-L)
- 0301-2115
- OpenAlex
- W1966317484
- Cited by
- 37
- References
- 36
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 5 · 2020: 1 · 2019: 3 · 2018: 3 · 2017: 2 · 2015: 3 · 2014: 4 · 2012: 3
- FWCI
- 1.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Obstetrics & Gynecology and Reproductive Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- SLAC National Accelerator Laboratory
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- bone marrow mesenchymal stem cells, estradiol, endometrial stromal cells, co-culture, cytokeratin, epithelial markers, endometriosis, stem cell differentiation
- MeSH terms
- Animals, Antigens, Differentiation, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Endometrium, Epithelial Cells, Estradiol, Estrogens, Female, Immunohistochemistry, Keratins, Mice, Inbred C57BL, Osmolar Concentration, Mucin-1, Coculture Techniques, Protein Isoforms, Mice, Stem Cell Niche, Mesenchymal Stem Cells
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