Cited by 298Peroxisome Proliferator-Activated ReceptorsFatty Acid Research and HealthCannabis and Cannabinoid Research

Anandamide induced PPARγ transcriptional activation and 3T3-L1 preadipocyte differentiation

Bouaboula, Monsif, Hilairet, Sandrine, Marchand, Jean, Fajas, Lluis, Fur, Gerard Le, Casellas, Pierre·2005·European Journal of Pharmacology

Abstract

We investigated the effects of anandamide on peroxisome proliferator-activated receptor gamma (PPARgamma) activity. In two different transactivation systems using either full-length or only the ligand binding domain of PPARgamma, we showed that anandamide, but not palmitoylethanolamide induced transcriptional activation of PPARgamma in a dose dependent manner with an EC50 of 8 microM. In addition, competition binding experiments showed that anandamide but not palmitoylethanolamide binds directly to PPAR-ligand binding domain. We also found that anandamide treatment induced 3T3-L1 fibroblast differentiation into adipocytes. Indeed, anandamide induced triglyceride droplet accumulation and the expression of PPARgamma responsive genes such as CCAAT enhancer binding protein alpha (C-EBPalpha), aP2, PerilipinA and Acrp30. Furthermore, the PPARgamma antagonist (GW9662) inhibited the anandamide-induced 3T3-L1 differentiation confirming that this is a PPARgamma-mediated process. Altogether, these data indicate that anandamide binds PPARgamma and induces cellular PPARgamma signaling.

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Crossref · 10.1016/j.ejphar.2005.05.032

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Authors
Bouaboula, M., Hilairet, S., Marchand, J., Fajas, L., Fur, G. L., & Casellas, P.
Affiliations
Sanofi (France) (FR)
Corresponding author
Casellas, Pierre
Venue
European Journal of Pharmacology
Publisher
Elsevier BV
Published
2005-07
Volume
517
Issue
3
Pages
174-181
ISSN
0014-2999 (ISSN-L)
OpenAlex
W2061169432
Cited by
298
References
43
Citations by year
2026: 4 · 2025: 8 · 2024: 9 · 2023: 10 · 2022: 7 · 2021: 25 · 2020: 14 · 2019: 14 · 2018: 15 · 2017: 13 · 2016: 14 · 2015: 16 · 2014: 19 · 2013: 17 · 2012: 17
FWCI
5.92
Type
journal article
Language
en
Open access
closed
Locations
  • European Journal of Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
Keywords
anandamide, PPARγ, transcriptional activation, adipogenesis, palmitoylethanolamide, GW9662, C/EBPα, AP-2, perilipin, adiponectin, endocannabinoids, triglyceride accumulation
MeSH terms
Perilipin-1, Pioglitazone, Troglitazone, Anilides, Animals, Arachidonic Acids, Binding Sites, Binding, Competitive, Carrier Proteins, Cell Differentiation, Cell Line, Chlorocebus aethiops, Chromans, Dose-Response Relationship, Drug, Fibroblasts, HeLa Cells, Humans, Luciferases, Phosphoproteins, Plasmids, Recombinant Fusion Proteins, Transfection, Blotting, Western, Transcriptional Activation, Gene Expression, Adipocytes, COS Cells, Reverse Transcriptase Polymerase Chain Reaction, CCAAT-Enhancer-Binding Protein-alpha, Intercellular Signaling Peptides and Proteins, 3T3-L1 Cells, Thiazolidinediones, PPAR gamma, Mice, Adiponectin, Polyunsaturated Alkamides, Cannabinoid Receptor Modulators, Endocannabinoids

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