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Cited by 16Muscle metabolism and nutritionExercise and Physiological ResponsesAdipose Tissue and Metabolism

Intramyocellular lipid content and lipogenic gene expression responses following a single bout of resistance type exercise differ between young and older men

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Tsintzas, Kostas, Stephens, Francis B., Snijders, Tim, Wall, Benjamin T., Cooper, Scott, Mallinson, Joanne, Verdijk, Lex B., van Loon, Luc J.C.·2017·Experimental Gerontology
DOI 10.1016/j.exger.2017.03.018
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Abstract

The aim of this study was to examine the temporal relationship between intramyocellular lipid (IMCL) content and the expression of genes associated with IMCL turnover, fat metabolism, and inflammation during recovery from an acute bout of resistance type exercise in old versus young men. Seven healthy young (23±2years, 77.2±2.9kg) and seven healthy older (72±1years, 79.3±4.9kg) males performed a single bout of resistance exercise involving 6 sets of 10 repetitions of leg press and 6 sets of 10 repetitions of leg extension at 75% one-repetition maximum (1-RM). Muscle biopsy samples were obtained before and 12, 24 and 48h after the completion of exercise and analysed for IMCL content and the expression of 48 genes. The subjects refrained from further heavy physical exercise and consumed a standardized diet for the entire experimental period. The IMCL content was ~2-fold higher at baseline and 12h post-exercise in old compared with young individuals. However, no differences between groups were apparent after 48h of recovery. There was higher expression of genes involved in fatty acid synthesis (FASN and PPARγ) during the first 24h of recovery. Differential responses to exercise were observed between groups for a number of genes indicating increased inflammatory response (IL6, IkBalpha, CREB1) and impaired fat metabolism and TCA cycle (LPL, ACAT1, SUCLG1) in older compared with younger individuals. A singe bout of resistance type exercise leads to molecular changes in skeletal muscle favouring reduced lipid oxidation, increased lipogenesis, and exaggerated inflammation during post-exercise recovery in the older compared with younger individuals, which may be indicative of a blunted response of IMCL turnover with ageing.

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Authors
Tsintzas, K., Stephens, F. B., Snijders, T., Wall, B. T., Cooper, S., Mallinson, J., Verdijk, L. B., & van Loon, L. J. C.
Affiliations
  • University of Nottingham (GB)
  • Maastricht University Medical Centre (NL)
  • University of Exeter (GB)
Corresponding author
Tsintzas, Kostas
ORCID
Venue
Experimental Gerontology
Publisher
Elsevier BV
Published
2017-07
Volume
93
Pages
36-45
ISSN
0531-5565 (ISSN-L)
DOI
10.1016/j.exger.2017.03.018
OpenAlex
W2604928479
Cited by
16
References
42
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 1
FWCI
1.31
Type
journal article
Language
en
Open access
Licence
Locations
  • Experimental Gerontology (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion, cc-by)
  • Open Research Exeter (University of Exeter) (acceptedVersion)
  • Research Bank (Australian Catholic University) (submittedVersion)
  • Nottingham ePrints (University of Nottingham) (submittedVersion, cc-by)
  • Figshare (submittedVersion)
  • Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
  • VUBIR (Vrije Universiteit Brussel) (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
intramyocellular lipid, resistance training, aging, skeletal muscle, lipogenesis, fatty acid synthesis, PPARγ, fatty acid synthase, inflammation, interleukin-6, IkBα, CREB1, fat metabolism, lipid oxidation, TCA cycle, post-exercise recovery, gene expression, older men
MeSH terms
Adult, Aged, Aging, Blood Glucose, Gene Expression Regulation, Humans, Insulin, Male, Exercise, Muscle, Skeletal, Inflammation Mediators, Lipogenesis, Lipid Metabolism, Young Adult

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