Cinnamaldehyde inhibits pro-inflammatory cytokines secretion from monocytes/macrophages through suppression of intracellular signaling
Abstract
We investigated the in vitro anti-inflammatory effects of Cinnamaldehyde, a cytokine production inhibitor isolated from an essential oil produced from the leaves of Cinnamomum osmophloeum Kaneh, and its mechanism of action. Although Cinnamaldehyde has been reported to have contact sensitizing properties at high concentration (mM), we found that low concentration of Cinnamaldehyde (muM) inhibited the secretion of interleukin-1beta and tumor necrosis factor alpha within lipopolysaccharide (LPS) or lipoteichoic acid (LTA) stimulated murine J774A.1 macrophages. Cinnamaldehyde also suppressed the production of these cytokines from LPS stimulated human blood monocytes derived primary macrophages and human THP-1 monocytes. Furthermore, Cinnamaldehyde also inhibited the production of prointerleukin-1beta within LPS or LTA stimulated human THP-1 monocytes. Reactive oxygen species release from LPS stimulated J774A.1 macrophages was reduced by Cinnamaldehyde. The phosphorylation of extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase 1/2 induced by LPS was also inhibited by Cinnamaldehyde; however, Cinnamaldehyde neither antagonize the binding of LPS to the cells nor alter the cell surface expression of toll-like receptor 4 and CD14. In addition, we also noted that Cinnamaldehyde appeared to elicit no cytotoxic effect upon J774A.1 macrophages under our experimental conditions, although Cinnamaldehyde reduced J774A.1 macrophages proliferation as analysed by MTT assay. Our current results have demonstrated the anti-oxidation and anti-inflammatory properties of Cinnamaldehyde that could provide the possibility for Cinnamaldehyde's future pharmaceutical application in the realm of immuno-modulation.
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Cinnamaldehyde inhibits secretion of IL-1β and TNF-α in LPS- or LTA-stimulated murine J774A.1 macrophages and suppresses these cytokines from LPS-stimulated human blood monocyte-derived primary macrophages and human THP-1 monocytes.
“Cinnamaldehyde, isolated from an essential oil produced from the leaves of Cinnamomum osmophloeum, was reported to inhibit the secretion of IL-1β and TNF-α within LPS or lipoteichoic acid (LTA) stimulated murine J774A.1 macrophages. Cinnamaldehyde also suppressed the production of these cytokines from LPS stimulated human blood monocytes derived primary macrophages and human THP-1 monocytes [111].”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [111]
Cinnamaldehyde-mediated inhibition of cytokine production may be at least in part due to reduction of ROS release and of JNK and ERK within LPS-stimulated J774A.1 macrophages.
“Cinnamaldehyde-mediated inhibition of cytokines production found by Chao et al. (2008) [111], and reported above, may be, at least on part, due to the reduction of ROS release as well as of those of JNK and ERK within LPS stimulated J774A.1 macrophages.”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [111]
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Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review
Miguel, M. G. (2010) · Molecules · doi:10.3390/molecules15129252 · Cited by 933 · see citing works
“Cinnamaldehyde-mediated inhibition of cytokines production found by Chao et al. (2008) [111], and reported above, may be, at least on part, due to the reduction of ROS release as well as of those of JNK and ERK within LPS stimulated J774A.1 macrophages.”
Background
- Authors
- Chao, L. K., Hua, K.-F., Hsu, H.-Y., Cheng, S.-S., Lin, I.-F., Chen, C.-J., Chen, S.-T., & Chang, S.-T.
- Affiliations
- China Medical University (TW)
- National Yang Ming University (TW)
- Taipei Municipal YangMing Hospital (TW)
- National Taiwan University (TW)
- Genomics Research Center, Academia Sinica (TW)
- Corresponding author
- Chang, Shang-Tzen
- Venue
- Food and Chemical Toxicology
- Publisher
- Elsevier BV
- Published
- 2008-01
- Volume
- 46
- Issue
- 1
- Pages
- 220-231
- ISSN
- 0278-6915 (ISSN-L)
- OpenAlex
- W2068917566
- Cited by
- 250
- References
- 45
- Citations by year
- 2026: 9 · 2025: 12 · 2024: 25 · 2023: 15 · 2022: 15 · 2021: 23 · 2020: 15 · 2019: 15 · 2018: 24 · 2017: 17 · 2016: 11 · 2015: 12 · 2014: 10 · 2013: 9 · 2012: 11
- FWCI
- 2.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food and Chemical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council (NSC 93-2314-B-010-003, NSC 94-2120-M-010-002, NSC-92-2313-B-002-042)
- Academia Sinica
- Ministry of Education (95A-C-D01-PPG-10)
- National Health Research Institutes (NHRI-EX93-9211SI)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- cinnamaldehyde, anti-inflammatory activity, inflammatory cytokines, IL-1β, TNF-α, macrophages, monocytes, lipopolysaccharide, lipoteichoic acid, reactive oxygen species, ERK1/2 phosphorylation, JNK1/2 phosphorylation, TLR4, CD14, Cinnamomum osmophloeum, essential oils, immunomodulation
- MeSH terms
- Acrolein, Cell Line, Cell Survival, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, Hydrogen Peroxide, Immunologic Factors, Lipopolysaccharides, Macrophages, Monocytes, Oils, Volatile, Signal Transduction, Free Radical Scavengers, Cytokines, Oxidants, Reactive Oxygen Species, Plant Leaves, Lipopolysaccharide Receptors, Mitogen-Activated Protein Kinases, Cinnamomum, Intracellular Signaling Peptides and Proteins, Toll-Like Receptors
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