Detection of mitochondrial biomarkers in eutopic endometria of endometriosis using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry
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Objectives To detect specific mitochondrial proteins in eutopic endometrial samples from women with and without endometriosis and to build diagnostic models.Design Eutopic endometrial samples from women with endometriosis (excluding adenomyosis) and women with benign indications as control were studied by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein-chip technology. After finding the biomarkers, the diagnostic model was evaluated and validated by leave-one cross-validation.Setting Collaborative investigation in an academic research environment.Patient(s) Twenty-four patients with endometriosis (excluding adenomyosis) and 29 patients with benign indications as control.Intervention(s) Surgical excision of eutopic endometrial biopsy of patients with endometriosis and controls.Main outcome measure(s) Mitochondrial protein expression.Result(s) Seventy-eight qualified mitochondrial protein peaks were detected, ten of them had a significant difference. Three combined potential biomarkers, with mass-to-charge ratios (m/z) of 15,334, 15,128, and 16,069, were found, and the diagnostic system distinguished endometriosis from control samples with a specificity of 86.2% and a sensitivity of 87.5%.Conclusion(s) We discovered potential mitochondrial biomarkers of eutopic endometrium in endometriosis and set up a diagnostic model. Further identification of the proteins we found will help to explain pathology, new diagnoses, and therapeutic approaches for endometriosis.
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Menstrual cycle phase of endometrial samples was not specified in several of the studies.
“Specifically, menstrual cycle phase of endometrial samples was not specified in several of the studies [132, 135], and only one randomly divided endometrial samples into training and test sets [131].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [135]
In the largest study to date involving a total of 53 endometrial samples, a panel of three differentially expressed peptide peaks (16.069 m/z, 15.334 m/z, and 15.128 m/z) diagnosed endometriosis (all rAFS stages) with 87.5% sensitivity and 86.2% specificity.
“In the largest study to date involving a total of 53 endometrial samples, a panel of three differentially expressed peptide peaks (16.069 m/z, 15.334 m/z, and 15.128 m/z) diagnosed endometriosis (all rAFS stages) with 87.5% sensitivity and 86.2% specificity [135].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [135]
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- Authors
- Ding, X., Wang, L., Ren, Y., & Zheng, W.
- Affiliations
- Second Affiliated Hospital of Zhejiang University (CN)
- Corresponding author
- Zheng, Wei
- Venue
- Fertility and Sterility
- Publisher
- Elsevier BV
- Published
- 2010-12
- Volume
- 94
- Issue
- 7
- Pages
- 2528-2530
- ISSN
- 0015-0282 (ISSN-L)
- OpenAlex
- W2033939941
- Cited by
- 24
- References
- 16
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 1 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 4 · 2013: 6 · 2012: 2
- FWCI
- 4.17
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Fertility and Sterility (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- endometriosis, eutopic endometrium, mitochondrial proteins, biomarkers, diagnostic model, protein microarrays, endometrial biopsy, differentially expressed proteins
- MeSH terms
- Adult, Diagnostic Techniques, Obstetrical and Gynecological, Endometriosis, Endometrium, Female, Humans, Mitochondria, Sensitivity and Specificity, Uterine Diseases, Reproducibility of Results, Biomarkers, Case-Control Studies, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Mitochondrial Proteins
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