Proteomics Analysis of Plasma for Early Diagnosis of Endometriosis
Abstract
OBJECTIVE: To test the hypothesis that differential surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein or peptide expression in plasma can be used in infertile women with or without pelvic pain to predict the presence of laparoscopically and histologically confirmed endometriosis, especially in the subpopulation with a normal preoperative gynecologic ultrasound examination. METHODS: Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry analysis was performed on 254 plasma samples obtained from 89 women without endometriosis and 165 women with endometriosis (histologically confirmed) undergoing laparoscopies for infertility with or without pelvic pain. Data were analyzed using least squares support vector machines and were divided randomly (100 times) into a training data set (70%) and a test data set (30%). RESULTS: Minimal-to-mild endometriosis was best predicted (sensitivity 75%, 95% confidence interval [CI] 63-89; specificity 86%, 95% CI 71-94; positive predictive value 83.6%, negative predictive value 78.3%) using a model based on five peptide and protein peaks (range 4.898-14.698 m/z) in menstrual phase samples. Moderate-to-severe endometriosis was best predicted (sensitivity 98%, 95% CI 84-100; specificity 81%, 95% CI 67-92; positive predictive value 74.4%, negative predictive value 98.6%) using a model based on five other peptide and protein peaks (range 2.189-7.457 m/z) in luteal phase samples. The peak with the highest intensity (2.189 m/z) was identified as a fibrinogen β-chain peptide. Ultrasonography-negative endometriosis was best predicted (sensitivity 88%, 95% CI 73-100; specificity 84%, 95% CI 71-96) using a model based on five peptide peaks (range 2.058-42.065 m/z) in menstrual phase samples. CONCLUSION: A noninvasive test using proteomic analysis of plasma samples obtained during the menstrual phase enabled the diagnosis of endometriosis undetectable by ultrasonography with high sensitivity and specificity. LEVEL OF EVIDENCE: II.
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A combination of 5 peptide peaks discovered by SELDI-TOF mass spectrometry detected endometriosis with sensitivity of 88% and specificity of 84% in the menstrual phase.
“A combination of 5 peptide peaks, discovered by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry, detected endometriosis with a sensitivity of 88% and a specificity of 84% in the menstrual phase [91].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [91]
Peptide fingerprinting using proteomic analysis of plasma may have utility as a noninvasive or semi-invasive diagnostic for endometriosis.
“Peptide fingerprinting using proteomic analysis of plasma may also have utility as a noninvasive or semi-invasive diagnostic for endometriosis.20”
From Defining Future Directions for Endometriosis Research: Workshop Report From the 2011 World Congress of Endometriosis in Montpellier, France (2013) · cites this paper as [20]
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- Authors
- Fassbender, A., Waelkens, E., Verbeeck, N., Kyama, C. M., Bokor, A., Vodolazkaia, A., Van de Plas, R., Meuleman, C., Peeraer, K., Tomassetti, C., Gevaert, O., Ojeda, F., De Moor, B., & DʼHooghe, T.
- Affiliations
- Fund for Scientific Research (BE)
- Semmelweis University (HU)
- ORCID
- Venue
- Obstetrics & Gynecology
- Published
- 2012-02
- Volume
- 119
- Issue
- 2, Part 1
- Pages
- 276-285
- ISSN
- 0029-7844 (ISSN-L)
- OpenAlex
- W2158537745
- Cited by
- 51
- References
- 30
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 6 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 7 · 2013: 12 · 2012: 2
- FWCI
- 10.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Obstetrics and Gynecology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- endometriosis, plasma proteomics, early diagnosis, infertility, pelvic pain, menstrual cycle phases, luteal phase, non-invasive biomarkers, least squares support vector machine, diagnostic accuracy
- MeSH terms
- Adult, Blood Proteins, Endometriosis, Female, Humans, Menstruation, Predictive Value of Tests, Severity of Illness Index, Ultrasonography, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Proteomics, Young Adult
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