Distinctive anti-inflammatory effects of resveratrol, dihydroresveratrol, and 3-(4-hydroxyphenyl)-propionic acid on DSS-induced colitis in pseudo-germ-free mice
Abstract
Resveratrol is a dietary polyphenol that interacts with gut microbiota to possess various biological activities. To identify the microbial metabolites of resveratrol, fresh feces from 12 volunteers were cultured in vitro. Their urine samples were collected after taking a commercial capsule containing 600 mg of resveratrol. Metabolites were characterized and quantified by UPLC-Q-Exactive plus orbitrap MS/MS. The results showed that dihydroresveratrol, 3-(4-hydroxyphenyl)-propionic acid, and lunularin were the major microbial metabolites of RSV with interindividual differences. 3-(4-Hydroxyphenyl)-propionic acid significantly attenuated the inflammatory response of LPS-treated RAW264.7 cells and DSS-induced colitis in antibiotics-treated pseudo-germ-free mice by regulating MAPK and NF-κB pathways. In contrast, dihydroresveratrol did not exhibit significant anti-inflammatory effects, and lunularin exhibited pro-inflammatory effects in cells. This study may help to better understand the health effects of resveratrol and its microbial metabolites.
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Dihydroresveratrol reduced expression of IL-6, IL-18, and IL-1β mRNA in RAW 264.7 cells at 50 μM.
“Dihydroresveratrol exhibited anti-inflammatory activity, reducing the expression of IL-6, IL-18, and IL-1β mRNA in RAW 264.7 cells, when treated with 50 μM of the compound [18,121].”
From Exploring the Lesser-Known Bioactive Natural Products of Plant Species of the Genus Cannabis L.: Alkaloids, Phenolic Compounds, and Their Therapeutic Potential (2025) · cites this paper as [121]
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Deng, Y., Guo, Y., Zhao, B., Wang, Y.-S., Jin, F., Fang, S., Yang, H.-K., Li, D.-Y., Mei, X., Lin, S., et al. (2026) · Materials & Design · doi:10.1016/j.matdes.2026.115680 · Cited by 1 · see citing works
Medicinal orchids as anti-inflammatory agents: Phytochemistry and molecular mechanisms of action
Padhee, S., Kerry, R. G., Champati, B. B., Mohanty, D., Mohanty, S., Dash, K. T., Acharya, R., Ray, A., Dash, B., Jena, S., et al. (2026) · South African Journal of Botany · doi:10.1016/j.sajb.2026.03.016 · Cited by 1 · see citing works
The Role of Polyphenols in Respiratory and Gut Health: From the Perspective of Gut‐Lung Axis
Kang, J., Du, Y., Hou, Y., Hao, L., Chen, Z., Xie, H., Zhang, C., & Li, L. (2026) · Phytotherapy Research · doi:10.1002/ptr.70379 · Cited by 1 · see citing works
Li, X., Cui, M., Zhu, L., Zhang, H. M., Guan, Y., Song, X., Zhao, M., Li, Z., Liao, F., Shi, L., et al. (2025) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-025-05320-2
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Resveratrol’s hepatic symphony: conducting a multi-stage intervention in liver cancer’s progression
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Self-citationXia, Y., Ni, R., He, F., Liu, F., Wang, T., Yang, Z., Li, C., Weng, H., Wu, D., Gao, N., et al. (2026) · Food Chemistry X · doi:10.1016/j.fochx.2026.104576
- Authors
- Zhang, B., Zhang, Y., Liu, X., Zhao, C., Yin, J., Li, X., Zhang, X., Wang, J., & Wang, S.
- Affiliations
- Nankai University (CN)
- Corresponding author
- Wang, Jin
- Wang, Shuo
- ORCID
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2023-01
- Volume
- 400
- Pages
- 133904
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W4293860862
- Cited by
- 47
- References
- 55
- Citations by year
- 2026: 9 · 2025: 15 · 2024: 14 · 2023: 7 · 2022: 2
- FWCI
- 5.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (32101962)
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- resveratrol, 3-(4-hydroxyphenyl)propionic acid, gut microbiota, microbial metabolites, DSS-induced colitis, anti-inflammatory activity, NF-κB signaling, MAPK signaling, RAW 264.7 macrophages, lipopolysaccharide-induced inflammation, interindividual variability
- MeSH terms
- Resveratrol, Animals, Anti-Inflammatory Agents, Anti-Bacterial Agents, Bibenzyls, Colitis, Lipopolysaccharides, Mice, Inbred C57BL, Phenols, Phenylpropionates, Propionates, Stilbenes, Dextran Sulfate, NF-kappa B, Mice, Tandem Mass Spectrometry, Polyphenols
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