Oxidative stress as a candidate mechanism for accelerated neuroectodermal differentiation due to trisomy 21
Open accessAbstract
The ubiquity of cognitive deficits and early onset Alzheimer's disease in Down syndrome (DS) has focused much DS iPSC-based research on neuron degeneration and regeneration. Despite reports of elevated oxidative stress in DS brains, few studies assess the impact of this oxidative burden on iPSC differentiation. Here, we evaluate cellular specific redox differences in DS and euploid iPSCs and neural progenitor cells (NPCs) during critical intermediate stages of differentiation. Despite successful generation of NPCs, our results indicate accelerated neuroectodermal differentiation of DS iPSCs compared to isogenic, euploid controls. Specifically, DS embryoid bodies (EBs) and neural rosettes prematurely develop with distinct morphological differences from controls. Additionally, we observed developmental stage-specific alterations in mitochondrial superoxide production and SOD1/2 abundance, coupled with modulations in thioredoxin, thioredoxin reductase, and peroxiredoxin isoforms. Disruption of intracellular redox state and its associated signaling has the potential to disrupt cellular differentiation and development in DS lending to DS-specific phenotypes.
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Trisomy 21 iPSCs show accelerated neural differentiation that correlates with disruption of intracellular redox state and its associated signaling.
“This study of trisomy 21 iPSCs reveals accelerated neural differentiation that correlates with disruption of intracellular redox state and its associated signaling.”
From Consequences of trisomy 21 for brain development in Down syndrome (2024) · cites this paper as [76]
Cites
Regulation of gliogenesis in the central nervous system by the JAK-STAT signaling pathway.
Bonni, A., Sun, Y., Nadal-Vicens, M., Bhatt, A. S., Frank, D., Rozovsky, I., Stahl, N., Yancopouloš, G., & Greenberg, M. (1997) · Science · doi:10.1126/science.278.5337.477 · Cited by 1,050 · see citing works
Identification and classification of chromosomal aberrations in human induced pluripotent stem cells
Mayshar, Y., Ben-David, U., Lavon, N., Biancotti, J.-C., Yakir, B., Clark, A. T., Plath, K., Lowry, W. E., & Benvenisty, N. (2010) · Cell stem cell · doi:10.1016/j.stem.2010.07.017 · Cited by 756 · see citing works
Apoptosis and increased generation of reactive oxygen species in Down's syndrome neurons in vitro
Busciglio, J., & Yankner, B. A. (1995) · Nature · doi:10.1038/378776a0 · Cited by 741 · see citing works
Chromosome 21 and Down syndrome: from genomics to pathophysiology
Antonarakis, S. E., Lyle, R., Dermitzakis, E. T., Reymond, A., & Deutsch, S. (2004) · Nature Reviews Genetics · doi:10.1038/nrg1448 · Cited by 703 · see citing works
A genetic cause of Alzheimer disease: mechanistic insights from Down syndrome
Wiseman, F. K., Al-Janabi, T., Hardy, J., Karmiloff-Smith, A., Nizetic, D., Tybulewicz, V. L. J., Fisher, E. M. C., & Strydom, A. (2015) · Nature Reviews Neuroscience · doi:10.1038/nrn3983 · Cited by 562 · see citing works
Almeida, M., Li, H., Martín-Millán, M., O’Brien, C. A., & Manolagas, S. C. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m702811200 · Cited by 546 · see citing works
Neuronal caspase-3 signaling: not only cell death
D’Amelio, M., Cavallucci, V., & Cecconi, F. (2009) · Cell Death and Differentiation · doi:10.1038/cdd.2009.180 · Cited by 526 · see citing works
Pax6 is a human neuroectoderm cell fate determinant
Zhang, X., Huang, C. T., Chen, J., Pankratz, M. T., Xi, J., Li, J., Yang, Y., LaVaute, T. M., Li, X.-J., Ayala, M., et al. (2010) · Cell stem cell · doi:10.1016/j.stem.2010.04.017 · Cited by 480 · see citing works
Function of reactive oxygen species during animal development: Passive or active?
Covarrubias, L., Hernández-García, D., Schnabel, D., Vidal, E. S., & Castro‐Obregón, S. (2008) · Developmental Biology · doi:10.1016/j.ydbio.2008.04.041 · Cited by 402 · see citing works
Sansom, S. N., Griffiths, D. S., Faedo, A., Kleinjan, D., Ruan, Y., Smith, J. C., van Heyningen, V., Rubenstein, J. L. R., & Livesey, F. J. (2009) · PLoS Genetics · doi:10.1371/journal.pgen.1000511 · Cited by 401 · see citing works
Nestin-expressing progenitor cells: function, identity and therapeutic implications
Bernal, A., & Arranz, L. (2018) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-018-2794-z · Cited by 394 · see citing works
Funato, Y., Michiue, T., Asashima, M., & Miki, H. (2006) · Nature Cell Biology · doi:10.1038/ncb1405 · Cited by 378 · see citing works
Genetic and Epigenetic Variations in iPSCs: Potential Causes and Implications for Application
Liang, G., & Zhang, Y. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.07.001 · Cited by 368 · see citing works
Davidson, K. C., Adams, A. M., Goodson, J. M., McDonald, C. E., Potter, J. C., Berndt, J. D., Biechele, T. L., Taylor, R. J., & Moon, R. T. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1118777109 · Cited by 338 · see citing works
Controlled, Scalable Embryonic Stem Cell Differentiation Culture
Dang, S. M., Gerecht, S., Chen, J., Itskovitz‐Eldor, J., & Zandstra, P. W. (2004) · Stem Cells · doi:10.1634/stemcells.22-3-275 · Cited by 314 · see citing works
Caspase Activity Mediates the Differentiation of Embryonic Stem Cells
Fujita, J., Crane, A. M., Souza, M. K., Déjosez, M., Kyba, M., Flavell, R. A., Thomson, J. A., & Zwaka, T. P. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.04.001 · Cited by 282 · see citing works
Dendritic atrophy in children with down’s syndrome.
Becker, L. E., Armstrong, D. L., & Chan, F. (1986) · Annals of Neurology · doi:10.1002/ana.410200413 · Cited by 279 · see citing works
Guidi, S., Bonasoni, P., Ceccarelli, C., Santini, D., Gualtieri, F., Ciani, E., & Bartesaghi, R. (2008) · Brain Pathology · doi:10.1111/j.1750-3639.2007.00113.x · Cited by 276 · see citing works
Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation
Bratt‐Leal, A. M., Carpenedo, R. L., & McDevitt, T. C. (2009) · Biotechnology Progress · doi:10.1002/btpr.139 · Cited by 272 · see citing works
Suzuki, S., Namiki, J., Shibata, S., Mastuzaki, Y., & Okano, H. (2010) · Journal of Histochemistry & Cytochemistry · doi:10.1369/jhc.2010.955609 · Cited by 236 · see citing works
Fok, E. Y. L., & Zandstra, P. W. (2005) · Stem Cells · doi:10.1634/stemcells.2005-0112 · Cited by 234 · see citing works
Neural stem cell differentiation is dependent upon endogenous caspase‐3 activity
Fernando, P., Brunette, S., & Megeney, L. A. (2005) · The FASEB Journal · doi:10.1096/fj.04-2981fje · Cited by 210 · see citing works
(1991) · PubMed · Cited by 209 · see citing works
β-Catenin signaling is required for neural differentiation of embryonic stem cells
Otero, J. J., Fu, W., Kan, L., Cuadra, A. E., & Kessler, J. A. (2004) · Development · doi:10.1242/dev.01218 · Cited by 203 · see citing works
Sahoo, T., Dzidic, N., Strecker, M. N., Commander, S., Travis, M. K., Doherty, C., Tyson, R. W., Mendoza, A., Stephenson, M. D., Dise, C. A., et al. (2016) · Genetics in Medicine · doi:10.1038/gim.2016.69 · Cited by 202 · see citing works
Rodent models in Down syndrome research: impact and future opportunities
Hérault, Y., Delabar, J. M., Fisher, E., Tybulewicz, V. L. J., Yu, E. Y., & Brault, V. (2017) · Disease Models & Mechanisms · doi:10.1242/dmm.029728 · Cited by 191 · see citing works
Biomarkers of oxidative stress are significantly elevated in Down syndrome
Jovanovic, S. V., Clements, D., & MacLeod, K. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00137-3 · Cited by 183 · see citing works
Adaptive Downregulation of Mitochondrial Function in Down Syndrome
Helguera, P. R., Seiglie, J., Rodríguez, J. A., Hanna, M., Helguera, G. F., & Busciglio, J. A. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2012.12.005 · Cited by 179 · see citing works
The roles of glutathione peroxidases during embryo development
Ufer, C. (2011) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2011.00012 · Cited by 158 · see citing works
Krieg, P. A. (2014) · Methods · doi:10.1016/j.ymeth.2014.03.033 · Cited by 143 · see citing works
Guo, Y., Chakraborty, S., Rajan, S. S., Wang, R., & Huang, F. (2010) · Stem Cells and Development · doi:10.1089/scd.2009.0313 · Cited by 136 · see citing works
Glutathione redox dynamics and expression of glutathione-related genes in the developing embryo
Timme‐Laragy, A. R., Goldstone, J. V., Imhoff, B. R., Stegeman, J. J., Hahn, M. E., & Hansen, J. M. (2013) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2013.06.011 · Cited by 134 · see citing works
Brickman, J. M., & Serup, P. (2016) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.259 · Cited by 129 · see citing works
Dendrites, dementia and the down syndrome
Takashima, S., Ieshima, A., Nakamura, H., & Becker, L. E. (1989) · Brain and Development · doi:10.1016/s0387-7604(89)80082-8 · Cited by 128 · see citing works
Down's syndrome: A pathology involving the lack of balance of reactive oxygen species
Kȩdziora, J., & Bartosz, G. (1988) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(88)90052-4 · Cited by 122 · see citing works
Hoffrogge, R., Mikkat, S., Scharf, C., Beyer, S., Christoph, H., Pahnke, J., Mix, E., Berth, M., Uhrmacher, A. M., Zubrzycki, I. Z., et al. (2006) · PROTEOMICS · doi:10.1002/pmic.200500556 · Cited by 122 · see citing works
Redox Control in Mammalian Embryo Development
Ufer, C., Wang, C. C., Borchert, A., Heydeck, D., & Kühn, H. (2010) · Antioxidants and Redox Signaling · doi:10.1089/ars.2009.3044 · Cited by 120 · see citing works
Hydrogen peroxide negatively modulates Wnt signaling through downregulation of β-catenin
Shin, S. Y., Kim, C. G., Jho, E., Rho, M.-S., Kim, Y. S., Kim, Y.-H., & Lee, Y. H. (2004) · Cancer Letters · doi:10.1016/j.canlet.2004.03.003 · Cited by 111 · see citing works
Beta-Amyloid, Oxidative Stress and Down Syndrome
Lott, I. T., Head, E., Doran, E. W., & Busciglio, J. A. (2006) · Current Alzheimer Research · doi:10.2174/156720506779025305 · Cited by 110 · see citing works
Neuroinflammation in the Aging Down Syndrome Brain; Lessons from Alzheimer's Disease
Wilcock, D. M. (2012) · Current Gerontology and Geriatrics Research · doi:10.1155/2012/170276 · Cited by 104 · see citing works
Yan, Y., Sabharwal, P., Rao, M., & Sockanathan, S. (2009) · Cell · doi:10.1016/j.cell.2009.06.042 · Cited by 103 · see citing works
Reactive oxygen metabolites and prooxidant status in children with Down's syndrome.
Carratelli, M., Porcaro, L., Ruscica, M., Simone, E. D., Bertelli, A., & Corsi, M. M. (2001) · PubMed · Cited by 101 · see citing works
Glutamine Metabolism Regulates the Pluripotency Transcription Factor OCT4
Marsboom, G., Zhang, G., Pohl-Avila, N. M., Zhang, Y., Yang, Y., Kang, H., Hao, B., Brunengraber, H., Malik, A. B., & Rehman, J. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.05.089 · Cited by 99 · see citing works
Piccoli, C., Izzo, A., Scrima, R., Bonfiglio, F., Manco, R., Negri, R., Quarato, G., Cela, O., Ripoli, M. C., Prisco, M., et al. (2012) · Human Molecular Genetics · doi:10.1093/hmg/dds529 · Cited by 96 · see citing works
Vertebrate-specific glutaredoxin is essential for brain development
Bräutigam, L., Schütte, L. D., Godoy, J. R., Prozorovski, T., Gellert, M., Hauptmann, G., Holmgren, A., Lillig, C. H., & Berndt, C. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1110085108 · Cited by 91 · see citing works
Gulesserian, T., Seidl, R., Hardmeier, R., Cairns, N. J., & Lübec, G. (2001) · Journal of Investigative Medicine · doi:10.2310/6650.2001.34089 · Cited by 90 · see citing works
Early oxidative stress in amniotic fluid of pregnancies with Down syndrome
Perrone, S., Longini, M., Bellieni, C. V., Centini, G., Kenanidis, A., Marco, L. D., Petraglia, F., & Buonocore, G. (2006) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2006.10.019 · Cited by 88 · see citing works
Redox regulation of Wnt signalling via nucleoredoxin
Funato, Y., & Miki, H. (2010) · Free Radical Research · doi:10.3109/10715761003610745 · Cited by 82 · see citing works
Oxidative stress promotes exit from the stem cell state and spontaneous neuronal differentiation
Hu, Q., Khanna, P., Wong, B. S. E., Heng, Z. S., Subhramanyam, C. S., Thanga, L. Z., Tan, S. W. S., & Baeg, G. H. (2017) · Oncotarget · doi:10.18632/oncotarget.23786 · Cited by 82 · see citing works
Redox stress and signaling during vertebrate embryonic development: Regulation and responses
Timme‐Laragy, A. R., Hahn, M. E., Hansen, J. M., Rastogi, A., & Roy, M. A. (2017) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2017.09.019 · Cited by 71 · see citing works
Maurer, M. H., Feldmann, R. E., Fütterer, C. D., Butlin, J., & Kuschinsky, W. (2004) · Neurochemical Research · doi:10.1023/b:nere.0000023600.25994.11 · Cited by 64 · see citing works
The burden of trisomy 21 disrupts the proteostasis network in Down syndrome
Aivazidis, S., Coughlan, C. M., Rauniyar, A. K., Jiang, H. L., Liggett, L. A., Maclean, K. N., & Roede, J. R. (2017) · PLoS ONE · doi:10.1371/journal.pone.0176307 · Cited by 62 · see citing works
Self-citationGlutathione metabolism and antioxidant enzymes in children with down syndrome
Pastore, A., Tozzi, G., Gaeta, L. M., Giannotti, A., Bertini, E., Federici, G., Digilio, M. C., & Piemonte, F. (2003) · The Journal of Pediatrics · doi:10.1067/mpd.2003.203 · Cited by 61 · see citing works
(2011) · PubMed · Cited by 57 · see citing works
Genomic Editing Tools to Model Human Diseases with Isogenic Pluripotent Stem Cells
Kim, H. S., Bernitz, J. M., Lee, D., & Lemischka, I. R. (2014) · Stem Cells and Development · doi:10.1089/scd.2014.0167 · Cited by 54 · see citing works
Calcium signaling mediates five types of cell morphological changes to form neural rosettes
Hříbková, H., Grabiec, M., Klemová, D., Slaninová, I., & Sun, Y.-M. (2018) · Journal of Cell Science · doi:10.1242/jcs.206896 · Cited by 50 · see citing works
Lee, H.-C., Tan, K.-L., Cheah, P.-S., & Ling, K. (2016) · Neural Plasticity · doi:10.1155/2016/7434191 · Cited by 49 · see citing works
Redox-regulated fate of neural stem progenitor cells
Prozorovski, T., Schneider, R., Berndt, C., Hartung, H., & Aktaş, O. (2015) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2015.01.022 · Cited by 43 · see citing works
Shin, J., London, J., LePecheur, M., Höger, H., Pollak, D. D., & Lübec, G. (2004) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.05.019 · Cited by 42 · see citing works
Dasgupta, A., Hughey, R. P., Lancin, P. A., Larue, L., & Moghe, P. V. (2005) · Biotechnology and Bioengineering · doi:10.1002/bit.20676 · Cited by 42 · see citing works
The Down Syndrome Cognition Project, Channell, M. M., Mattie, L. J., Hamilton, D. R., Capone, G. T., Mahone, E. M., Sherman, S. L., Rosser, T. C., Reeves, R. H., & Kalb, L. G. (2021) · Journal of Neurodevelopmental Disorders · doi:10.1186/s11689-021-09365-2 · Cited by 41 · see citing works
Cytogenetic analysis of first trimester pregnancy loss
Petracchi, F., Colaci, D., Igarzábal, L., & Gadow, E. C. (2008) · International Journal of Gynecology & Obstetrics · doi:10.1016/j.ijgo.2008.10.014 · Cited by 39 · see citing works
Henry, M. P., Hawkins, J. R., Boyle, J., & Bridger, J. M. (2019) · Frontiers in Genetics · doi:10.3389/fgene.2018.00623 · Cited by 37 · see citing works
Yao, Y., Lu, Y., Chen, W., Jiang, Y., Cheng, T., Ma, Y., Lu, L., & Dai, W. H. (2014) · PLoS ONE · doi:10.1371/journal.pone.0086620 · Cited by 34 · see citing works
Chen, K. G., Mallon, B. S., Johnson, K. R., Hamilton, R. S., McKay, R. D. G., & Robey, P. G. (2014) · Stem Cell Research · doi:10.1016/j.scr.2014.02.002 · Cited by 34 · see citing works
Trisomy 21 results in modest impacts on mitochondrial function and central carbon metabolism
Anderson, C. C., Marentette, J., Prutton, K. M., Rauniyar, A. K., Reisz, J. A., D’Alessandro, A., Maclean, K. N., Saba, L. M., & Roede, J. R. (2021) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2021.06.003 · Cited by 25 · see citing works
Self-citationMoore, R. H., Tao, W., Meng, Y. D., Smith, E. R., & Xu, X. (2014) · Biology Open · doi:10.1242/bio.20146254 · Cited by 21 · see citing works
Moore, R. H., Radice, G. L., Dominis, M., & Kemler, R. (1999) · The International Journal of Developmental Biology · doi:10.1387/ijdb.10707907 · Cited by 21 · see citing works
Free radical damage in Down's syndrome brain
Reynolds, G. P., & CUTTS, A. J. (1993) · Biochemical Society Transactions · doi:10.1042/bst021221s · Cited by 17 · see citing works
C21orf91 Regulates Oligodendroglial Precursor Cell Fate—A Switch in the Glial Lineage?
Reiche, L., Göttle, P., Lane, L., Duek, P. D., Park, M., Azim, K., Schütte, J., Manousi, A., Schira‐Heinen, J., & Küry, P. (2021) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2021.653075 · Cited by 14 · see citing works
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Russo, M. L., Sousa, A. M. M., & Bhattacharyya, A. (2024) · Nature Reviews Neuroscience · doi:10.1038/s41583-024-00866-2 · Cited by 28 · see citing works
Watson, L. A., & Meharena, H. S. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.1198129 · Cited by 13 · see citing works
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Elangovan, A., Babu, H. W. S., Iyer, M., Gopalakrishnan, A. V., & Vellingiri, B. (2023) · Ageing Research Reviews · doi:10.1016/j.arr.2023.101930 · Cited by 11 · see citing works
Prutton, K. M., Marentette, J., Maclean, K. N., & Roede, J. R. (2023) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2023.01.009 · Cited by 9 · see citing works
Self-citationTeng, C., Wei, J., Zhu, L., Chen, A., & Chen, X. (2026) · Journal of Translational Medicine · doi:10.1186/s12967-026-08104-2 · Cited by 7 · see citing works
Mouli, K., Liopo, A. V., Suva, L. J., Olson, K. R., McHugh, E. A., Tour, J. M., Derry, P. J., & Kent, T. A. (2024) · Antioxidants · doi:10.3390/antiox13111361 · Cited by 6 · see citing works
Cindrić, A., Vučković, F., Murray, A., Klarić, T., Alić, I., Petrović, D. J., Krištić, J., Nižetić, D., & Lauc, G. (2024) · BBA Advances · doi:10.1016/j.bbadva.2024.100137 · Cited by 4 · see citing works
PIGK defects induce apoptosis in Purkinje cells and acceleration of neuroectodermal differentiation
Chen, S., You, J., Zhou, X., Li, Y., Liu, F., Teng, Y., Teng, H., Li, Y., Liang, D., Li, Z., et al. (2024) · Cell Death and Disease · doi:10.1038/s41419-024-07201-8 · Cited by 3 · see citing works
Mouli, K., Liopo, A. V., Wang, H., Suva, L. J., Olson, K. R., Derry, P. J., & Kent, T. A. (2026) · Scientific Reports · doi:10.1038/s41598-026-45627-6 · Cited by 1 · see citing works
Jandy, M., Hu, H., Liu, Y., & Bhattacharyya, A. (2025) · Springer eBooks · doi:10.1007/978-3-031-78611-2_7
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- Authors
- Prutton, K. M., Marentette, J. O., Leifheit, B. A., Esquer, H., LaBarbera, D. V., Anderson, C. C., Maclean, K. N., & Roede, J. R.
- Affiliations
- University of Colorado Anschutz (US)
- Corresponding author
- Roede, James R.
- ORCID
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2022-06
- Volume
- 186
- Pages
- 32-42
- ISSN
- 0891-5849 (ISSN-L)
- OpenAlex
- W4229048962
- Cited by
- 13
- References
- 70
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 4 · 2023: 3
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (am, 345 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Free Radical Biology and Medicine (acceptedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Environmental Health Sciences (R01ES027593, R25ES025476)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Down syndrome and intellectual disability research
- Keywords
- Down syndrome, oxidative stress, neuroectodermal differentiation, induced pluripotent stem cells, neural progenitor cells, embryoid bodies, neural rosettes, mitochondrial superoxide, superoxide dismutase, thioredoxin, thioredoxin reductase, peroxiredoxin, redox state, iPSC differentiation
- MeSH terms
- Cell Differentiation, Cells, Cultured, Down Syndrome, Humans, Oxidative Stress, Induced Pluripotent Stem Cells
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