Activation of wnt/β-catenin signaling pathway down regulated osteogenic differentiation of bone marrow-derived stem cells in an anhidrotic ectodermal dysplasia patient with EDA/EDAR/EDARADD mutation
Open accessAbstract
Objective To explore the mechanism by which the Wnt/β-catenin pathway induces osteogenic differentiation of bone marrow-derived stem cells (BMSCs) in anhidrotic ectodermal dysplasia (AED) with an Ectodysplasin A (EDA)/EDA receptor (EDAR)/EDARADD mutation. Methods An AED patient served as the AED group, whereas the other patients without AED were included in the normal group. Peripheral venous blood collected from the AED patient was subjected to whole-genome resequencing. BMSCs from the mandible of patients with AED and normal individuals were isolated and cultured in vitro . Cell proliferation assay was performed to compare the growth speed of BMSCs between the AED and normal groups. CHIR-99021, an activator of the Wnt/β-catenin pathway and XAV-939, an inhibitor, was used to manage BMSCs in an osteogenic environment in both groups. The expression of β-catenin was detected by quantitative polymerase chain reaction, while that of RUNX2 was detected by western blotting. Alizarin red was used for staining. Results A novel mutation (c.152T > A in EDA) and two known mutations (c.1109T > C in EDAR and c.27G > A in EDARADD) were identified. The growth rate in the normal group was higher than that in the AED group. In the normal group, the number and size of calcified nodes and the expression of RUNX-2 increased with CHIR-99021 treatment, which could be inhibited by XAV-939. In contrast, CHIR-99021 inhibited osteogenesis in the AED group and this effect was promoted by XAV-939. Conclusion Activation of the Wnt/β-catenin pathway downregulates osteogenesis of BMSCs in AED patients with EDA/EDAR/EDARADD gene mutations. Further investigation in more AED patients is required, given the wide range of mutations involved in AED.
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In ED patients, limited local blood supply, reduced bone remodeling capacity, and rapid graft resorption often hinder long-term implant stability.
“However, limited local blood supply, reduced bone remodeling capacity, and rapid graft resorption often hinder long-term implant stability in ED patients.”
From Implant-supported fixed prosthodontic treatment for ectodermal dysplasia: a case report (2026) · cites this paper as [20]
The Results (Case analysis of the AED patient; page not provided) report implant failures in one patient, and the Discussion links impaired osteogenic differentiation to reduced osseointegration as a possible cause. The paper does not report limited local blood supply or rapid graft resorption, and its findings in one patient do not establish that these problems often hinder implant stability in ED patients. (LLM, conf=0.87)
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Implant-supported fixed prosthodontic treatment for ectodermal dysplasia: a case report
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- Authors
- Bao, D.-Y., Yang, Y., Tong, X., & Qin, H.-Y.
- Affiliations
- Nanjing Drum Tower Hospital (CN)
- Nanjing University (CN)
- Venue
- Heliyon
- Publisher
- Elsevier BV
- Published
- 2024-01
- Volume
- 10
- Issue
- 1
- Pages
- e23057
- ISSN
- 2405-8440 (ISSN-L)
- PMC
- PMC10758735
- OpenAlex
- W4389503581
- Cited by
- 3
- References
- 48
- Citations by year
- 2026: 2 · 2025: 1
- FWCI
- 0.27
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Heliyon (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (81200770)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- hypohidrotic ectodermal dysplasia, Wnt/β-catenin signaling, osteogenic differentiation, bone marrow-derived stem cells, EDAR, EDARADD, CHIR99021, XAV939, Runx2, osteogenesis inhibition
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