In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from Enteromorpha prolifera
Abstract
Water-soluble sulfated polysaccharides extracted from Enteromorpha prolifera and fractionated using ion-exchange chromatography (crude, F(1), F(2) and F(3) fractions) were investigated to determine their in vitro and in vivo immunomodulatory activities. The sulfated polysaccharides, especially the F(1) and F(2) fractions, stimulated a macrophage cell line, Raw 264.7, inducing considerable nitric oxide (NO) and various cytokine production via up-regulated mRNA expression. The in vivo experiment results show that the sulfated polysaccharides (the crude and F(2) fractions) significantly increased Con A-induced splenocyte proliferation, revealing their potential comitogenic activity. In addition, IFN-γ and IL-2 secretions were considerably increased by the F(2) fraction without altering the release of IL-4 and IL-5. This implies that the F(2) fraction can activate T cells by up-regulating Th-1 response and that Th-1 cells might be the main target cells of the F(2) fraction. These in vitro and in vivo results suggest that the sulfated polysaccharides are strong immunostimulators.
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Water-soluble sulfated polysaccharide fractions from Enteromorpha prolifera significantly increased ConA-induced splenocyte proliferation.
“These polysaccharides significantly increased ConA-induced splenocyte proliferation and induced the production of various cytokines via up-regulated m-RNA expression [109].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [109]
Water-soluble sulfated polysaccharide fractions from Enteromorpha prolifera stimulate immunity in vivo and in vitro.
“In vivo and in vitro stimulation of immunity was indicated as the action of water-soluble sulfated polysaccharide fractions from Enteromorpha prolifera.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [109]
Water-soluble sulfated polysaccharide fractions from Enteromorpha prolifera induced production of various cytokines via up-regulated mRNA expression.
“These polysaccharides significantly increased ConA-induced splenocyte proliferation and induced the production of various cytokines via up-regulated m-RNA expression [109].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [109]
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Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
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- Authors
- Kim, J.-K., Cho, M. L., Karnjanapratum, S., Shin, I.-S., & You, S. G.
- Affiliations
- Daegu Catholic University (KR)
- Gangneung–Wonju National University (KR)
- Corresponding author
- You, Sang Guan
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2011-12
- Volume
- 49
- Issue
- 5
- Pages
- 1051-1058
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W1976517559
- Cited by
- 219
- References
- 27
- Citations by year
- 2026: 7 · 2025: 15 · 2024: 20 · 2023: 10 · 2022: 21 · 2021: 26 · 2020: 29 · 2019: 19 · 2018: 17 · 2017: 15 · 2016: 13 · 2015: 8 · 2014: 5 · 2013: 7 · 2012: 7
- FWCI
- 9.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Science and Technology (RTI05-01-02)
- Ministry of Knowledge Economy
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Medicine › Pharmacology › Fungal Biology and Applications
- Keywords
- sulfated polysaccharides, Enteromorpha prolifera, seaweed polysaccharides, immunomodulation, immunostimulants, macrophage activation, nitric oxide, cytokine production, splenocyte proliferation, Th1 response, interferon-gamma, interleukin-2, ion-exchange fractionation
- MeSH terms
- Animals, Cell Line, Chromatography, Ion Exchange, Female, Immunity, Innate, Immunologic Factors, Interferon-gamma, Interleukins, Lymphocyte Activation, Macrophages, Mice, Inbred BALB C, Nitric Oxide, Polysaccharides, Sulfates, Gene Expression, Th1 Cells, Reverse Transcriptase Polymerase Chain Reaction, Ulva, Cell Proliferation, Mice, Immunomodulation
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