Immunomodulatory activity of polysaccharides isolated from Strongylocentrotus nudus eggs
Abstract
Our previous work showed that SEP, a novel glucan isolated from the eggs of sea urchins, Strongylocentrotus nudus, had remarkable anti-tumor activity. To elucidate the mechanism of the anti-tumor activity, the immunomodulatory activity of SEP was investigated. The in vivo experiment results showed that SEP remarkably enhanced spleen and thymus index in S180-bearing mice, and also stimulated ConA-induced splenocyte proliferation. Immunomodulatory activity assay in vitro indicated SEP could significantly enhance the mouse splenocyte proliferation in a dose-dependent manner. According to comitogenic activity tests, SEP showed significant comitogenic activities and adjuvant properties. We also demonstrated that SEP had a unique mode of immunostimulation with regard to its cell-type specificity. In other words, SEP markedly stimulated B and T cell proliferation, however the influence on B cells was greatly weaker than that on T cells. IL-2, TNF-alpha, and IFN-gamma mRNA expression was upregulated after the mouse splenocytes were treated by SEP, indicating that Th1 cell was the primary cellular target affected by SEP on T lymphocyte. SEP enhanced production of nitric oxide (NO), upregulated mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages in a dose-dependent manner. In addition, SEP did not show direct toxicity to tumor cells. Consequently, the anti-tumor effect of SEP was related to stimulating host immunity/enhancing the immune system functions, which may mainly result from SEP activating lymphocytes and macrophages and stimulating secretion of some cytokines.
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Polysaccharide SEP isolated from the eggs of the sea urchin Strongylocentrotus nudus effectively inhibited the growth of S180 tumor and hepatocellular carcinoma in vivo via activation of lymphocytes and macrophages, amplification of B and T cell proliferation, and increased secretion of IL-2, TNF-α and IFN-γ.
“Polysaccharide SEP isolated from the eggs of the sea urchin Strongylocentrotus nudus effectively inhibited the growth of S180 tumor and the hepatocellular carcinoma in vivo via the activation of lymphocytes and macrophages, amplification of B and T cell proliferation, and increased secretion of such cytokines as IL-2, TNF-α and IFN-γ [138,139,140,141].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [139]
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- Authors
- Liu, C., Xi, T., Lin, Q., Xing, Y., Ye, L., Luo, X., & Wang, F.
- Affiliations
- Shandong University (CN)
- China Pharmaceutical University (CN)
- Corresponding author
- Liu, Chunhui
- Xi, Tao
- Venue
- International Immunopharmacology
- Publisher
- Elsevier BV
- Published
- 2008-12
- Volume
- 8
- Issue
- 13-14
- Pages
- 1835-1841
- ISSN
- 1567-5769 (ISSN-L)
- OpenAlex
- W2050234460
- Cited by
- 65
- References
- 23
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 1 · 2015: 1 · 2014: 5 · 2013: 6 · 2012: 4
- FWCI
- 9.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Immunopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- Strongylocentrotus nudus, sea urchin eggs, polysaccharides, immunomodulation, anticancer activity, splenocyte proliferation, adjuvant effect, Th1 cells, cytokine expression, inducible nitric oxide synthase, nitric oxide, peritoneal macrophages
- MeSH terms
- Adjuvants, Immunologic, Animals, Antineoplastic Agents, B-Lymphocytes, HeLa Cells, Humans, Macrophage Activation, Male, Mice, Inbred BALB C, Neoplasms, Nitric Oxide, Polymyxin B, Polysaccharides, Spleen, T-Lymphocytes, Cytokines, Macrophages, Peritoneal, Th1 Cells, HL-60 Cells, Cell Line, Tumor, Strongylocentrotus, Cell Proliferation, Mice, Nitric Oxide Synthase Type II
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