Cited by 43Probiotics and Fermented FoodsPolysaccharides Composition and ApplicationsReproductive tract infections research

Probiotic characteristics of exopolysaccharides-producing Lactobacillus isolated from some traditional Malaysian fermented foods

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Khalil, Eilaf Suliman, Manap, Mohd Yazid, Mustafa, Shuhaimi, Amid, Mehrnoush, Alhelli, Amaal Mohammed, Aljoubori, Ahmed·2018·CyTA - Journal of Food

Abstract

This study aims to evaluate the probiotic properties of Lactobacillus strains producing exopolysaccharides (EPS) isolated from different Malaysian fermented foods. Thirty lactobacilli species were isolated, and six of these (TAP1, PIC7, BU11, BU14, TAP16, and TEMP9) were shown to exhibit probiotic characteristics with the potential to produce EPS. The isolates were evaluated for their tolerance to low pH, bile salts, antimicrobial potential, and cell surface properties. The strains were identified using API50CHL and 16S rRNA gene sequences as Lactobacillus belonged to the species fermentum and plantarum. The Lactobacillus strains exhibited high survivability tolerance to low pH and bile salts (above 90%). All strains exhibited auto-aggregation (33.63–59.63%), co-aggregation (24.37–41.9%), and hydrophobicity (16–65.77%) ability. The strains displayed no transferrable antibiotics and antagonistic activity against pathogenic bacteria. Therefore, it indicates that Lactobacillus strains could act as promising probiotic candidates.

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Crossref · 10.1080/19476337.2017.1401007

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  • LAB are associated with several health benefits such as improvement of gastrointestinal tract conditions, antibacterial and antifungal activities, antiallergic and antioxidant properties, lowering cholesterol levels, and immunomodulatory activities.

    “The LAB are associated with several health benefits such as improvement of gastrointestinal tract conditions, antibacterial and antifungal activities, antiallergic and antioxidant properties, and lowering cholesterol levels and immunomodulatory activities [12, 13].”

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Authors
Khalil, E. S., Manap, M. Y., Mustafa, S., Amid, M., Alhelli, A. M., & Aljoubori, A.
Affiliations
Universiti Putra Malaysia (MY)
Corresponding author
Manap, Mohd Yazid
Venue
CyTA - Journal of Food
Published
2018-01
Volume
16
Issue
1
Pages
287-298
ISSN
  • 1947-6337 (ISSN-L)
  • 1947-6345
OpenAlex
W2783600692
Cited by
43
References
74
Citations by year
2026: 6 · 2025: 9 · 2024: 7 · 2023: 6 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 7 · 2018: 1
FWCI
2.82
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • CyTA - Journal of Food (publishedVersion, cc-by)
  • Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Ministry of Higher Education, Malaysia (No. 5030200)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Keywords
probiotics, Lactobacillus, exopolysaccharides, fermented foods, acid resistance, bile salt tolerance, self-aggregation, co-aggregation, hydrophobicity, antimicrobial activity, Lactobacillus plantarum, Lactobacillus fermentum, cell surface properties

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