Probiotic characteristics of exopolysaccharides-producing Lactobacillus isolated from some traditional Malaysian fermented foods
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This study aims to evaluate the probiotic properties of Lactobacillus strains producing exopolysaccharides (EPS) isolated from different Malaysian fermented foods. Thirty lactobacilli species were isolated, and six of these (TAP1, PIC7, BU11, BU14, TAP16, and TEMP9) were shown to exhibit probiotic characteristics with the potential to produce EPS. The isolates were evaluated for their tolerance to low pH, bile salts, antimicrobial potential, and cell surface properties. The strains were identified using API50CHL and 16S rRNA gene sequences as Lactobacillus belonged to the species fermentum and plantarum. The Lactobacillus strains exhibited high survivability tolerance to low pH and bile salts (above 90%). All strains exhibited auto-aggregation (33.63–59.63%), co-aggregation (24.37–41.9%), and hydrophobicity (16–65.77%) ability. The strains displayed no transferrable antibiotics and antagonistic activity against pathogenic bacteria. Therefore, it indicates that Lactobacillus strains could act as promising probiotic candidates.
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LAB are associated with several health benefits such as improvement of gastrointestinal tract conditions, antibacterial and antifungal activities, antiallergic and antioxidant properties, lowering cholesterol levels, and immunomodulatory activities.
“The LAB are associated with several health benefits such as improvement of gastrointestinal tract conditions, antibacterial and antifungal activities, antiallergic and antioxidant properties, and lowering cholesterol levels and immunomodulatory activities [12, 13].”
From Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk (2023) · cites this paper as [12]
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- Authors
- Khalil, E. S., Manap, M. Y., Mustafa, S., Amid, M., Alhelli, A. M., & Aljoubori, A.
- Affiliations
- Universiti Putra Malaysia (MY)
- Corresponding author
- Manap, Mohd Yazid
- Venue
- CyTA - Journal of Food
- Publisher
- Informa UK Limited
- Published
- 2018-01
- Volume
- 16
- Issue
- 1
- Pages
- 287-298
- ISSN
- 1947-6337 (ISSN-L)
- 1947-6345
- OpenAlex
- W2783600692
- Cited by
- 43
- References
- 74
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 7 · 2023: 6 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 7 · 2018: 1
- FWCI
- 2.82
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- CyTA - Journal of Food (publishedVersion, cc-by)
- Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministry of Higher Education, Malaysia (No. 5030200)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- probiotics, Lactobacillus, exopolysaccharides, fermented foods, acid resistance, bile salt tolerance, self-aggregation, co-aggregation, hydrophobicity, antimicrobial activity, Lactobacillus plantarum, Lactobacillus fermentum, cell surface properties
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