Characterization of a Novel Exopolysaccharide with Antitumor Activity from Lactobacillus plantarum 70810
Abstract
Three methods were used to prepare the cell-bound exopolysaccharides (c-EPS) of Lactobacillus plantarum 70810, and the maximum yield (64.17 mg/mL) was obtained by ultrasonic extraction. After anion exchange and gel filtration chromatography, the c-EPS was fractionated as a single peak with a molecular weight of 169.6 kD. Its structural characteristics were investigated by gas chromatography (GC), methylation, Fourier-transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) analysis. Based on obtained data, the novel c-EPS was found to be a galactan containing a backbone of α-D-(1→6)-linked galactcosyl, β-D-(1→4)-linked galactcosyl, β-D-(1→2,3)-linked galactcosyl residues and a tail end of β-D-(1→)-linked galactcosyl residues. Preliminary in vitro tests revealed that c-EPS significantly inhibited the proliferation of HepG-2, BGC-823, especially HT-29 tumor cells. Our results suggested that the c-EPS produced by L. plantarum 70810 might be suitable for use as functional foods and natural antitumor drugs.
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The type and origin of the strains, the culture conditions, and the composition of the medium can influence the content of the monosaccharides in the EPSs produced by LAB.
“The type and origin of the strains, the culture conditions, and the composition of the medium can influence the content of the monosaccharides in the EPSs produced by LAB [46].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [46]
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- Authors
- Wang, K., Li, W., Rui, X., Chen, X., Jiang, M., & Dong, M.
- Affiliations
- Nanjing Agricultural University (CN)
- Corresponding author
- Dong, Mingsheng
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2014-02
- Volume
- 63
- Pages
- 133-139
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2067569075
- Cited by
- 325
- References
- 32
- Citations by year
- 2026: 15 · 2025: 25 · 2024: 30 · 2023: 34 · 2022: 37 · 2021: 34 · 2020: 34 · 2019: 27 · 2018: 23 · 2017: 19 · 2016: 19 · 2015: 17 · 2014: 11
- FWCI
- 23.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- exopolysaccharides, Lactobacillus plantarum, anticancer activity, galactan, polysaccharide structure, ultrasound-assisted extraction, HepG2 cells, BGC-823 cells, HT-29 cells, functional food ingredients
- MeSH terms
- Humans, Neoplasms, Polysaccharides, Bacterial, Lactiplantibacillus plantarum, Cell Proliferation, Hep G2 Cells
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