Cited by 325Polysaccharides Composition and ApplicationsProbiotics and Fermented FoodsPolysaccharides and Plant Cell Walls

Characterization of a Novel Exopolysaccharide with Antitumor Activity from Lactobacillus plantarum 70810

Wang, Kun, Li, Wei, Rui, Xin, Chen, Xiaohong, Jiang, Mei, Dong, Mingsheng·2014·International Journal of Biological Macromolecules

Abstract

Three methods were used to prepare the cell-bound exopolysaccharides (c-EPS) of Lactobacillus plantarum 70810, and the maximum yield (64.17 mg/mL) was obtained by ultrasonic extraction. After anion exchange and gel filtration chromatography, the c-EPS was fractionated as a single peak with a molecular weight of 169.6 kD. Its structural characteristics were investigated by gas chromatography (GC), methylation, Fourier-transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) analysis. Based on obtained data, the novel c-EPS was found to be a galactan containing a backbone of α-D-(1→6)-linked galactcosyl, β-D-(1→4)-linked galactcosyl, β-D-(1→2,3)-linked galactcosyl residues and a tail end of β-D-(1→)-linked galactcosyl residues. Preliminary in vitro tests revealed that c-EPS significantly inhibited the proliferation of HepG-2, BGC-823, especially HT-29 tumor cells. Our results suggested that the c-EPS produced by L. plantarum 70810 might be suitable for use as functional foods and natural antitumor drugs.

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Crossref · 10.1016/j.ijbiomac.2013.10.036

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Authors
Wang, K., Li, W., Rui, X., Chen, X., Jiang, M., & Dong, M.
Affiliations
Nanjing Agricultural University (CN)
Corresponding author
Dong, Mingsheng
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2014-02
Volume
63
Pages
133-139
ISSN
0141-8130 (ISSN-L)
OpenAlex
W2067569075
Cited by
325
References
32
Citations by year
2026: 15 · 2025: 25 · 2024: 30 · 2023: 34 · 2022: 37 · 2021: 34 · 2020: 34 · 2019: 27 · 2018: 23 · 2017: 19 · 2016: 19 · 2015: 17 · 2014: 11
FWCI
23.17
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Keywords
exopolysaccharides, Lactobacillus plantarum, anticancer activity, galactan, polysaccharide structure, ultrasound-assisted extraction, HepG2 cells, BGC-823 cells, HT-29 cells, functional food ingredients
MeSH terms
Humans, Neoplasms, Polysaccharides, Bacterial, Lactiplantibacillus plantarum, Cell Proliferation, Hep G2 Cells

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