Characterization, Bioactivities, and Rheological Properties of Exopolysaccharide Produced by Novel Probiotic Lactobacillus plantarum C70 Isolated from Camel Milk
Abstract
Various industries highly regard the functionalities and bioactivities of bacterial polysaccharides. We aimed to characterize the exopolysaccharide (EPS) produced by novel probiotic Lactobacillus plantarum C70 (accession number KX881779) isolated from camel milk and to investigate its bioactivities and rheological properties. EPS-C70 had a weight-average molecular weight (Mw) of 3.8 × 10 5 Da. Arabinose (13.3%), mannose (7.1%), glucose (74.6%), and galactose (5.0%) were the major monosaccharides constituents. EPS-C70 had two endothermic peaks at 76.95 °C and 158.76 °C corresponding to glass transition (T g) and melting point (T m), respectively. Zeta potential and particle size of EPS-C70 were -330.71 mV and 525.5 nm, respectively. DPPH and ABTS of EPS-C70 were 75.91% and 49.42% at 10 mg/mL concentration, respectively. The cytotoxic activities against colon cancer and breast cancer lines were 88.1% and 73.1% at concentration 10 mg/mL, respectively. EPS-C70 exhibited shear-thinning behaviour. Salts and pH values had a significant impact on the rheological properties of EPS-C70.
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EPS-C70 produced by Lpb. plantarum isolated from camel milk showed good inhibitory activity towards food-borne pathogens, including Staphylococcus aureus and E. coli.
“EPS-C70 produced by Lpb. plantarum isolated from camel milk showed a good inhibitory activity towards food-borne pathogens, including Staphylococcus aureus and E. coli [144].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [144]
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- Authors
- Ayyash, M., Abu-Jdayil, B., Itsaranuwat, P., Galiwango, E., Tamiello-Rosa, C., Abdullah, H., Esposito, G., Hunashal, Y., Obaid, R. S., & Hamed, F.
- Affiliations
- United Arab Emirates University (AE)
- Mahasarakham University (TH)
- New York University Abu Dhabi (AE)
- University of Sharjah (AE)
- Corresponding author
- Ayyash, Mutamed
- Itsaranuwat, Pariyaporn
- ORCID
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2020-02
- Volume
- 144
- Pages
- 938-946
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2982299340
- Cited by
- 167
- References
- 39
- Citations by year
- 2026: 20 · 2025: 27 · 2024: 33 · 2023: 26 · 2022: 33 · 2021: 17 · 2020: 11
- FWCI
- 11.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- United Arab Emirates University (G00002839)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Animal Diversity and Health Studies
- Keywords
- exopolysaccharides, Lactobacillus plantarum, camel milk, probiotic bacteria, polysaccharide characterization, monosaccharide composition, thermal properties, antioxidant activity, cytotoxicity, colon cancer cells, breast cancer cells, shear-thinning rheology
- MeSH terms
- alpha-Amylases, alpha-Glucosidases, Animals, Biphenyl Compounds, Camelus, Elasticity, Milk, Monosaccharides, Picrates, Polysaccharides, Bacterial, Rheology, Sulfonic Acids, Temperature, Viscosity, Probiotics, Lactiplantibacillus plantarum, Benzothiazoles, Glycoside Hydrolase Inhibitors
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