Cited by 167Polysaccharides Composition and ApplicationsProbiotics and Fermented FoodsAnimal Diversity and Health Studies

Characterization, Bioactivities, and Rheological Properties of Exopolysaccharide Produced by Novel Probiotic Lactobacillus plantarum C70 Isolated from Camel Milk

Ayyash, Mutamed, Abu-Jdayil, Basim, Itsaranuwat, Pariyaporn, Galiwango, Emmanuel, Tamiello-Rosa, Camila, Abdullah, Hassan, Esposito, Gennaro, Hunashal, Yamanappa +2 more·2020·International Journal of Biological Macromolecules

Abstract

Various industries highly regard the functionalities and bioactivities of bacterial polysaccharides. We aimed to characterize the exopolysaccharide (EPS) produced by novel probiotic Lactobacillus plantarum C70 (accession number KX881779) isolated from camel milk and to investigate its bioactivities and rheological properties. EPS-C70 had a weight-average molecular weight (Mw) of 3.8 × 10 5 Da. Arabinose (13.3%), mannose (7.1%), glucose (74.6%), and galactose (5.0%) were the major monosaccharides constituents. EPS-C70 had two endothermic peaks at 76.95 °C and 158.76 °C corresponding to glass transition (T g) and melting point (T m), respectively. Zeta potential and particle size of EPS-C70 were -330.71 mV and 525.5 nm, respectively. DPPH and ABTS of EPS-C70 were 75.91% and 49.42% at 10 mg/mL concentration, respectively. The cytotoxic activities against colon cancer and breast cancer lines were 88.1% and 73.1% at concentration 10 mg/mL, respectively. EPS-C70 exhibited shear-thinning behaviour. Salts and pH values had a significant impact on the rheological properties of EPS-C70.

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Crossref · 10.1016/j.ijbiomac.2019.09.171

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  • EPS-C70 produced by Lpb. plantarum isolated from camel milk showed good inhibitory activity towards food-borne pathogens, including Staphylococcus aureus and E. coli.

    “EPS-C70 produced by Lpb. plantarum isolated from camel milk showed a good inhibitory activity towards food-borne pathogens, including Staphylococcus aureus and E. coli [144].”

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Authors
Ayyash, M., Abu-Jdayil, B., Itsaranuwat, P., Galiwango, E., Tamiello-Rosa, C., Abdullah, H., Esposito, G., Hunashal, Y., Obaid, R. S., & Hamed, F.
Affiliations
  • United Arab Emirates University (AE)
  • Mahasarakham University (TH)
  • New York University Abu Dhabi (AE)
  • University of Sharjah (AE)
Corresponding author
  • Ayyash, Mutamed
  • Itsaranuwat, Pariyaporn
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2020-02
Volume
144
Pages
938-946
ISSN
0141-8130 (ISSN-L)
OpenAlex
W2982299340
Cited by
167
References
39
Citations by year
2026: 20 · 2025: 27 · 2024: 33 · 2023: 26 · 2022: 33 · 2021: 17 · 2020: 11
FWCI
11.76
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
Funders
United Arab Emirates University (G00002839)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Animal Diversity and Health Studies
Keywords
exopolysaccharides, Lactobacillus plantarum, camel milk, probiotic bacteria, polysaccharide characterization, monosaccharide composition, thermal properties, antioxidant activity, cytotoxicity, colon cancer cells, breast cancer cells, shear-thinning rheology
MeSH terms
alpha-Amylases, alpha-Glucosidases, Animals, Biphenyl Compounds, Camelus, Elasticity, Milk, Monosaccharides, Picrates, Polysaccharides, Bacterial, Rheology, Sulfonic Acids, Temperature, Viscosity, Probiotics, Lactiplantibacillus plantarum, Benzothiazoles, Glycoside Hydrolase Inhibitors

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