Activation of Several Key Components of the Epidermal Differentiation Pathway in Cattle Following Infestation with the Cattle Tick, Rhipicephalus (Boophilus) Microplus
Open accessAbstract
The cattle tick, Rhipicephalus (Boophilus) microplus, and the diseases it transmits pose a persistent threat to tropical beef production. Genetic selection of host resistance has become the method of choice for non-chemical control of cattle tick. Previous studies have suggested that larval stages are most susceptible to host resistance mechanisms. To gain insights into the molecular basis of host resistance that occurs during R. microplus attachment, we assessed the abundance of proteins (by isobaric tag for relative and absolute quantitation (iTRAQ) and Western blot analyses) and mRNAs (by quantitative reverse transcription PCR (qRT-PCR)) in skin adjacent to tick bite sites from high tick-resistant (HR) and low tick-resistant (LR) Belmont Red cattle following challenge with cattle tick. We showed substantially higher expression of the basal epidermal keratins KRT5 and KRT14, the lipid processing protein, lipocalin 9 (LCN9), the epidermal barrier catalysing enzyme transglutaminase 1 (TGM1), and the transcriptional regulator B lymphocyte-induced maturation protein 1 (Blimp1) in HR skin. Our data reveals the essential role of the epidermal permeability barrier in conferring greater resistance of cattle to tick infestation, and suggest that the physical structure of the epidermal layers of the skin may represent the first line of defence against ectoparasite invasion.
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Keratin genes KRT5 and KRT14 are highly expressed in cattle populations exhibiting high tick tolerance.
“Notably, the keratin genes KRT5 and KRT14 are highly expressed in cattle populations exhibiting high tick tolerance [119].”
From The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution (2026) · cites this paper as [119]
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Taye, M., Lee, W., Caetano-Anolles, K., Dessie, T., Cho, S., Jong Oh, S., Lee, H.-K., & Kim, H. (2018) · Cogent Food & Agriculture · doi:10.1080/23311932.2018.1552552 · Cited by 26 · see citing works
The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution
Liu, Y., Dao, W., Gao, R., Fan, X., Yang, R., & Miao, Y. (2026) · Animals · doi:10.3390/ani16050776 · Cited by 1 · see citing works
- Authors
- Kongsuwan, K., Josh, P., Colgrave, M. L., Bagnall, N. H., Gough, J., Burns, B., & Pearson, R.
- Affiliations
- Commonwealth Scientific and Industrial Research Organisation (AU)
- Department of Primary Industries (AU)
- Corresponding author
- Kongsuwan, Kritaya
- Venue
- International Journal for Parasitology
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 40
- Issue
- 4
- Pages
- 499-507
- ISSN
- 0020-7519 (ISSN-L)
- OpenAlex
- W1974554445
- Cited by
- 48
- References
- 52
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 7 · 2021: 3 · 2020: 3 · 2019: 5 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 5 · 2012: 2
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International Journal for Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Queensland Department of Agriculture and Fisheries archive of scientific and research publications (Queensland Department of Agriculture and Fisheries) (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
- Keywords
- Rhipicephalus microplus, cattle ticks, epidermal differentiation, epidermal permeability barrier, transglutaminase 1, Blimp-1, skin proteome
- MeSH terms
- Animals, Cattle, Cattle Diseases, Ectoparasitic Infestations, Immunity, Innate, RNA, Messenger, Skin, Blotting, Western, Reverse Transcriptase Polymerase Chain Reaction, Proteome, Gene Expression Profiling, Rhipicephalus
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