Effective inhibition of a Strongylocentrotus nudus eggs polysaccharide against hepatocellular carcinoma is mediated via immunoregulation in vivo
Abstract
This study was aimed at evaluating the inhibitory effect of a polysaccharide that was isolated from Strongylocentrotus nudus eggs (SEP) against hepatocellular carcinoma in H22-bearing mice and elucidating its immunological mechanisms by determining its effects on the growth of transplanted tumors and immune response in H22-bearing mice. ICR mice inoculated with mouse hepatoma carcinoma cell lines H22 were treated with SEP at doses of 4, 8, 16 mg/kg/d for 12 days. The effects of SEP were measured via the growth of the transplanted tumors, splenocyte proliferation, T lymphocytes counts, CTL activity, the production of cytokines from splenocytes and the levels of serum Ig in tumor-bearing mice. In addition, the effects of SEP on Erk phosphorylation in mouse splenocytes and on the transcriptional activity of NFAT in Jurkat T cells were also investigated. Our results showed that SEP significantly inhibited the growth of transplanted tumors in mice. SEP could not only remarkably enhance splenocyte proliferation, CD4(+) and CD8(+) T cell numbers as well as CTL activity, but it also elevated IL-2 and TNF-α secretion as well as IgA, IgM and IgG levels in the serum. Furthermore, the activation of Erk phosphorylation and the NFAT promoter by SEP promoted the transcription and expression of downstream gene IL-2. In conclusion, our study demonstrates that SEP effectively inhibits hepatocellular carcinoma in vivo via enhancement of host immune system function, and it could be a potential therapeutic drug for hepatocarcinoma.
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Polysaccharide SEP isolated from the eggs of the sea urchin Strongylocentrotus nudus effectively inhibited the growth of S180 tumor and hepatocellular carcinoma in vivo via activation of lymphocytes and macrophages, amplification of B and T cell proliferation, and increased secretion of IL-2, TNF-α and IFN-γ.
“Polysaccharide SEP isolated from the eggs of the sea urchin Strongylocentrotus nudus effectively inhibited the growth of S180 tumor and the hepatocellular carcinoma in vivo via the activation of lymphocytes and macrophages, amplification of B and T cell proliferation, and increased secretion of such cytokines as IL-2, TNF-α and IFN-γ [138,139,140,141].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [140]
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Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
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- Authors
- Wang, M., Wang, H., Tang, Y., Kang, D., Gao, Y., Ke, M., Dou, J., Xi, T., & Zhou, C.
- Affiliations
- China Pharmaceutical University (CN)
- Corresponding author
- Xi, Tao
- Zhou, Changlin
- Venue
- Immunology Letters
- Publisher
- Elsevier BV
- Published
- 2011-12
- Volume
- 141
- Issue
- 1
- Pages
- 74-82
- ISSN
- 0165-2478 (ISSN-L)
- OpenAlex
- W1981004339
- Cited by
- 36
- References
- 41
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2020: 2 · 2019: 1 · 2018: 5 · 2017: 4 · 2016: 5 · 2015: 2 · 2014: 3 · 2013: 3 · 2012: 2
- FWCI
- 3.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Immunology Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education of the People's Republic of China
- Government of Jiangsu Province
- State Administration of Foreign Experts Affairs (111-2-07)
- National Major Science and Technology Projects of China (2009ZX09103-655)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- hepatocellular carcinoma, antitumor polysaccharide, immune regulation, H22 hepatocellular carcinoma, splenocyte proliferation, T cells, cytotoxic T lymphocytes, cytokines, interleukin-2, TNF-α, ERK1/2 phosphorylation, NFAT transcription factors, IgA, IgM, IgG, anticancer natural products
- MeSH terms
- Adjuvants, Immunologic, Animals, Antineoplastic Agents, Carcinoma, Hepatocellular, Immunoglobulins, Interleukin-2, Liver Neoplasms, Male, Polysaccharides, Spleen, T-Lymphocytes, Tumor Necrosis Factor-alpha, Cell Line, Tumor, Strongylocentrotus, Cell Proliferation, Mice
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