Cited by 80T-cell and B-cell ImmunologyDevelopmental Biology and Gene RegulationHematopoietic Stem Cell Transplantation

T/B lineage choice occurs prior to intrathymic Notch signaling

Harman, Benjamin C., Jenkinson, William E., Parnell, Sonia M., Rossi, Simona W., Jenkinson, Eric J., Anderson, Graham·2005·Blood

Abstract

Commitment of hemopoietic progenitors to the T-cell lineage is a crucial requirement for T-cell development, yet the timing and developmental cues regulating this process remain controversial. Here we have devised a technique to analyze the T-cell/B-cell lineage potential of precursors that have been recruited to the fetal mouse thymus but which have yet to contact the thymic epithelial microenvironment. We show that lymphoid progenitors arriving at the thymus are not bipotent T/B precursors, and provide evidence that intrathymic Notch signaling is not the mechanism determining T/B lineage choice in migrant precursors. Rather, we provide evidence that Notch signaling influences T/B lineage choice in lymphoid precursors through interactions with defined stromal components within the fetal liver. Collectively, our data redefine our understanding of the role and timing of Notch signaling in relation to lineage choices in lymphoid precursors.

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Registry

Crossref · 10.1182/blood-2004-12-4881

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25 of 25 references verified0 citation errors · 0 not checkable

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  • This difference is now understood to be intrinsically programmed and due to an altered pathway of differentiation in the first-wave cells, which results in T-cell lineage commitment even before entry into the thymus.

    “This is now understood to be intrinsically programmed (136) and due to an altered pathway of differentiation in the first-wave cells, which results in T-cell lineage commitment even before entry into the thymus (137–139).”

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Authors
Harman, B. C., Jenkinson, W. E., Parnell, S. M., Rossi, S. W., Jenkinson, E. J., & Anderson, G.
Affiliations
University of Birmingham (GB)
Venue
Blood
Published
2005-08-01
Volume
106
Issue
3
Pages
886-892
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
OpenAlex
W2007923700
Cited by
80
References
25
Citations by year
2025: 1 · 2024: 3 · 2023: 1 · 2022: 1 · 2019: 1 · 2018: 2 · 2016: 6 · 2015: 3 · 2014: 1 · 2013: 3 · 2012: 6
FWCI
4.70
Type
journal article
Language
en
Open access
closed
Locations
  • Blood (publishedVersion)
  • PubMed (publishedVersion)
  • University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
Keywords
T cell lineage commitment, B cell lineage commitment, Notch signaling, thymus, thymic stromal cells, fetal liver, lymphoid progenitors, T cell development, hematopoietic progenitor differentiation
MeSH terms
Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases, Animals, B-Lymphocytes, Cell Communication, Epithelial Cells, Fetus, Liver, Membrane Proteins, Mice, Inbred BALB C, Phosphoric Monoester Hydrolases, T-Lymphocytes, Thymus Gland, Stromal Cells, Mice, Knockout, Coculture Techniques, Cell Lineage, Mice, Receptors, Notch

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