Cited by 244Immune Response and InflammationAdvanced Biosensing Techniques and ApplicationsClinical Laboratory Practices and Quality Control

Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli

Open access
Duffy, Darragh, Rouilly, Vincent, Libri, Valentina, Hasan, Milena, Beitz, Benoit, David, Mikael, Urrutia, Alejandra, Bisiaux, Aurélie +41 more·2014·Immunity

Abstract

Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1α as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing.

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Crossref · 10.1016/j.immuni.2014.03.002

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Authors
Duffy, D., Rouilly, V., Libri, V., Hasan, M., Beitz, B., David, M., Urrutia, A., Bisiaux, A., LaBrie, S. T., Dubois, A., Boneca, I. G., Delval, C., Thomas, S., Rogge, L., Schmolz, M., Quintana-Murci, L., Albert, M. L., Abel, L., Alcover, A., Bousso, P., Cumano, A., Daëron, M., Delval, C., Demangel, C., Deriano, L., Di Santo, J., Dromer, F., Eberl, G., Enninga, J., Freitas, A., Gomperts-Boneca, I., Hercberg, S., Lantz, O., Leclerc, C., Mouquet, H., Pellegrini, S., Pol, S., Rogge, L., Sakuntabhai, A., Schwartz, O., Schwikowski, B., Shorte, S., Soumelis, V., Tangy, F., Tartour, E., Toubert, A., Ungeheuer, M.-N., Quintana-Murci, L., & Albert, M. L.
Affiliations
  • Inserm (FR)
  • Institut Pasteur (FR)
  • Myriad Genetics (US)
  • Centre d’Immunologie de Marseille-Luminy (FR)
  • Department of Genomes & Genetics (FR)
  • Hôpital Cochin (FR)
  • Centre de Recherche sur les Liens Sociaux (FR)
  • Hôpital Saint-Louis (FR)
Venue
Immunity
Publisher
Elsevier BV
Published
2014-03
Volume
40
Issue
3
Pages
436-450
ISSN
1074-7613 (ISSN-L)
OpenAlex
W1984830992
Cited by
242
References
85
Citations by year
2026: 16 · 2025: 14 · 2024: 23 · 2023: 17 · 2022: 34 · 2021: 23 · 2020: 21 · 2019: 18 · 2018: 18 · 2017: 19 · 2016: 13 · 2015: 20 · 2014: 6
FWCI
8.83
Type
journal article
Language
en
Open access
Locations
  • Immunity (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
Keywords
innate immune response, adaptive immune response, cytokine signature, chemokines, interleukin-1 alpha, pre-analytical errors, T cell activation, immune response variability
MeSH terms
Antigens, Humans, Immunity, Innate, Reference Values, Monitoring, Immunologic, Reproducibility of Results, Cytokines, Inflammation Mediators, Adaptive Immunity, Healthy Volunteers

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