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hECA: The cell-centric assembly of a cell atlas

Chen, Sijie, Luo, Yanting, Gao, Haoxiang, Li, Fanhong, Chen, Yixin, Li, Jiaqi, You, Renke, Hao, Minsheng, Bian, Haiyang, Xi, Xi, Li, Wenrui, Li, Weiyu, Ye, Mingli, Meng, Qiuchen, Zou, Ziheng, Li, Chen, Li, Haochen, Zhang, Yangyuan, Cui, Yanfei, Wei, Lei, Chen, Fufeng, Wang, Xiaowo, Lv, Hairong, Hua, Kui, Jiang, Rui, Zhang, Xuegong·2022·iScience
DOI 10.1016/j.isci.2022.104318PMC PMC9114628

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Graphical abstract CC BY · PubMed Central
Figure 1 Overview of hECA. Scattered data are assembled into the ensemble cell atlas using a unified informatics framework The framework includes three key components uGT, uHAF, and ECAUGT. They made hECA the first cell-centric assembled cell atlases with structured indexing and support for combinatorial searching. Based on these components, three novel functions were built on hECA: “in data” cell sorting, quantitative portraiture, and customizable reference creation. See also Figures S5, S6, and S7.
Figure 1 Overview of hECA. Scattered data are assembled into the ensemble cell atlas using a unified informatics framework The framework includes three key components uGT, uHAF, and ECAUGT. They made hECA the first cell-centric assembled cell atlases with structured indexing and support for combinatorial searching. Based on these components, three novel functions were built on hECA: “in data” cell sorting, quantitative portraiture, and customizable reference creation. See also Figures S5, S6, and S7.CC BY · PubMed Central
Figure 2 The agile construction of a draft T-cell metabolic landscape across multiple organs from hECA (A) Workflow of the in data cell sorting from hECA to build the agile T cell atlas. (B) Subtypes of selected T cells are displayed on UMAP. DN T: Double negative T cell, CD8+ Tc: CD8+ Cytotoxic T cell, CD8+ Trm: CD8+ resident memory T cell, CD4+ Th1: CD4+ T helper cell type 1, CD4+ Tem: CD4+ effector memory T cell, CD4+ Tcm: CD4+ central memory T cell. (C) Organ origins of selected T cells organ origin displayed on UMAP. (D) Gene expression signatures of the identified T cell subtypes. The color bar represents average expression level of cell type related markers with colors gray to red indicating expression low to high. The dot size represents percentage of cells expressing the marker within subtypes. (E–F) Heatmaps showing z-scores of activity scores of major metabolic pathways of the T cell subtypes in multiple organs. (E) for CD4+ T cells and (F) for CD8+ T cells. Each row in the heatmap corresponds to one selected term in the KEGG metabolism pathway database, and each column corresponds to one T cell subcluster. See also Figures S1 and S2.
Figure 2 The agile construction of a draft T-cell metabolic landscape across multiple organs from hECA (A) Workflow of the in data cell sorting from hECA to build the agile T cell atlas. (B) Subtypes of selected T cells are displayed on UMAP. DN T: Double negative T cell, CD8+ Tc: CD8+ Cytotoxic T cell, CD8+ Trm: CD8+ resident memory T cell, CD4+ Th1: CD4+ T helper cell type 1, CD4+ Tem: CD4+ effector memory T cell, CD4+ Tcm: CD4+ central memory T cell. (C) Organ origins of selected T cells organ origin displayed on UMAP. (D) Gene expression signatures of the identified T cell subtypes. The color bar represents average expression level of cell type related markers with colors gray to red indicating expression low to high. The dot size represents percentage of cells expressing the marker within subtypes. (E–F) Heatmaps showing z-scores of activity scores of major metabolic pathways of the T cell subtypes in multiple organs. (E) for CD4+ T cells and (F) for CD8+ T cells. Each row in the heatmap corresponds to one selected term in the KEGG metabolism pathway database, and each column corresponds to one T cell subcluster. See also Figures S1 and S2.CC BY · PubMed Central
Figure 3 In data experiments with hECA facilitating discoveries of side effects of targeted drugs (A) The diagram of using in data cell sorting to predict targets and off-targets of targeted therapy. Red dots and blue dots in the human body represent the intended target sites and side-effect sites, respectively. The red and blue dots in the UMAP represent the intended treatment cells and side-effect cells, respectively. (B) Visualization of CD19+ cells (expression>0.1) in UMAP, colored by organ origins of cells. CD19 is the target gene of the targeted therapy. (C) Visualization of CD19 expression levels of those CD19+ cells. (D) Visualization of CD79A expression levels of those CD19+ cells. CD79A is a marker for B cells. (E) Visualization of CD248 expression levels of those CD19+ cells. CD248 is a marker for pericytes. (F) Visualization of CD22+ (expression>0.1) cells in UMAP, colored by organ origin of cells. CD22 is the target gene of the targeted therapy. (G) Visualization of CD22 expression levels of those CD19+ cells. (H) Visualization of CD79A expression levels of those CD22+ cells. CD79A is a marker for B cells. (I) Visualization of OLIG2 expression levels of those CD22+ cells. OLIG2 is a marker for oligodendrocytes. The color bars in (C–E) represent expression levels of CD19, CD79A, and CD248, and the color bars in (G–I) represent expression levels of CD22, CD79A, and OLIG2, respectively, with colors gray to red indicating expression low to high. The red and blue ellipses in (D–E) and (H–I) line out the target cells and off-target cells, respectively. See also Figures S3 and S4.
Figure 3 In data experiments with hECA facilitating discoveries of side effects of targeted drugs (A) The diagram of using in data cell sorting to predict targets and off-targets of targeted therapy. Red dots and blue dots in the human body represent the intended target sites and side-effect sites, respectively. The red and blue dots in the UMAP represent the intended treatment cells and side-effect cells, respectively. (B) Visualization of CD19+ cells (expression>0.1) in UMAP, colored by organ origins of cells. CD19 is the target gene of the targeted therapy. (C) Visualization of CD19 expression levels of those CD19+ cells. (D) Visualization of CD79A expression levels of those CD19+ cells. CD79A is a marker for B cells. (E) Visualization of CD248 expression levels of those CD19+ cells. CD248 is a marker for pericytes. (F) Visualization of CD22+ (expression>0.1) cells in UMAP, colored by organ origin of cells. CD22 is the target gene of the targeted therapy. (G) Visualization of CD22 expression levels of those CD19+ cells. (H) Visualization of CD79A expression levels of those CD22+ cells. CD79A is a marker for B cells. (I) Visualization of OLIG2 expression levels of those CD22+ cells. OLIG2 is a marker for oligodendrocytes. The color bars in (C–E) represent expression levels of CD19, CD79A, and CD248, and the color bars in (G–I) represent expression levels of CD22, CD79A, and OLIG2, respectively, with colors gray to red indicating expression low to high. The red and blue ellipses in (D–E) and (H–I) line out the target cells and off-target cells, respectively. See also Figures S3 and S4.CC BY · PubMed Central

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Metadata

Authors
Chen, S., Luo, Y., Gao, H., Li, F., Chen, Y., Li, J., You, R., Hao, M., Bian, H., Xi, X., Li, W., Li, W., Ye, M., Meng, Q., Zou, Z., Li, C., Li, H., Zhang, Y., Cui, Y., Wei, L., Chen, F., Wang, X., Lv, H., Hua, K., Jiang, R., & Zhang, X.
ORCID
Venue
iScience
Publisher
Elsevier BV
Published
2022-05
Volume
25
Issue
5
Pages
104318
ISSN
2589-0042
DOI
10.1016/j.isci.2022.104318
PMC
PMC9114628
Cited by
48
References
23
Language
en
Licence
Funders
  • National Natural Science Foundation of China (32000453, 42050101, 61721003, 62050178)
  • National Key Research and Development Program of China (2021YFF1200900)

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