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CRISPR/Cas: A powerful tool for gene function study and crop improvement
Open accessAbstract
It is a long-standing goal of scientists and breeders to precisely control a gene for studying its function as well as improving crop yield, quality, and tolerance to various environmental stresses. The discovery and modification of CRISPR/Cas system, a nature-occurred gene editing tool, opens an era for studying gene function and precision crop breeding. In this review, we first introduce the brief history of CRISPR/Cas discovery followed the mechanism and application of CRISPR/Cas system on gene function study and crop improvement. Currently, CRISPR/Cas genome editing has been becoming a mature cutting-edge biotechnological tool for crop improvement that already used in many different traits in crops, including pathogen resistance, abiotic tolerance, plant development and morphology and even secondary metabolism and fiber development. Finally, we point out the major issues associating with CRISPR/Cas system and the future research directions. Key Scientific Concepts of Review: CRISPR/Cas9 system is a robust and powerful biotechnological tool for targeting an individual DNA and RNA sequence in the genome. It can be used to target a sequence for gene knockin, knockout and replacement as well as monitoring and regulating gene expression at the genome and epigenome levels by binding a specific sequence. Agrobacterium-mediated method is still the major and efficient method for delivering CRISPR/Cas regents into targeted plant cells. However, other delivery methods, such as virus-mediated method, have been developed and enhanced the application potentials of CRISPR/Cas9-based crop improvement. PAM requirement offers the CRISPR/Cas9-targted genetic loci and also limits the application of CRISPR/Cas9. Discovering new Cas proteins and modifying current Cas enzymes play an important role in CRISPR/Cas9-based genome editing. Developing a better CRISPR/Cas9 system, including the delivery system and the methods eliminating off-target effects, and finding key/master genes for controlling crop growth and development is two major directions for CRISPR/Cas9-based crop improvement.
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![Fig. 2 Application of CRISPR/Cas genome editing in crop improvement, CRISPR/Cas is a robust tool and have huge potentials on crop improvement. Though targeting an individual DNA sequence, CRISPR/Cas can be used to add and/or change the expression of an individual gene that controls plant tolerance to abiotic and biotic stresses as well as plant growth and development. CRISPR/Cas also can change the secondary metabolism for controlling the biosynthesis of protein, carbohydrate, oil, and functional components. Partial figures are modified from previous publications [74], [108].](/paper-figures/6f08ea3b35d948ec97e53b01adce3ae0/crispr-cas-a-powerful-tool-for-gene-function-study-and-crop-improvement-figure-2.jpg)
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“| Delivery methods improvement | Application in vivo [95–105] | AAV: Last for years in terminally differentiated cell types | May cause accumulation of off-target mutations |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [101]
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In the citing paper
“| Delivery methods improvement | Application in vivo [95–105] | LNP: Can be quickly degraded in vivo | Eliminate off-target effects |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [101]
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“Designing sgRNA is more convenient, programmable and cost-effective than designing DNA binding domains, thus CRISPR/Cas9 is more favored than ZFNs and TALENs and has revolutionized the biotechnology field (Zhang et al., 2021; Tran et al., 2022).”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Zhang et al., 2021
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“Designing sgRNA is more convenient, programmable and cost-effective than designing DNA binding domains, thus CRISPR/Cas9 is more favored than ZFNs and TALENs and has revolutionized the biotechnology field (Zhang et al., 2021; Tran et al., 2022).”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Zhang et al., 2021
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In the citing paper
“| Delivery methods improvement | Application in vivo [95–105] | AAV: Last for years in terminally differentiated cell types | May cause accumulation of off-target mutations |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [101]
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In the citing paper
“| Delivery methods improvement | Application in vivo [95–105] | LNP: Can be quickly degraded in vivo | Eliminate off-target effects |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [101]
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · cited 2×
“If the system binds to the promoter region, the CRISPR/dCas system will block transcription factor and RNA polymerase (RNAP) binding to the promoter region and inhibit transcription initiation; otherwise, if the CRISPR/dCas system binds to the coding region, it will block RNAP binding and inhibit transcription elongat…”
BackgroundSearch-and-replace genome editing without double-strand breaks or donor DNA
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696 · cited 3×
“At the same time, if a transcription activator binds to the dCas9 protein, the CRISPR/dCas system will enhance the expression of the targeted genes [68].”
BackgroundInfluentialGenetic screens in human cells using the CRISPR-Cas9 system
Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014) · Science · doi:10.1126/science.1246981 · Cited by 2,856
Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769
Research works printed but not linked: 3
- Martyn, 1991, Resistance to races 0, 1, and 2 of Fusarium wilt of watermelon in Citrullus sp, PI-296341 -FR., 26, 429
- Zhang, 2016, Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA, Nat Commun, 7, 12617, 10.1038/ncomms12617 · DOI
- Hu, 2018, Evolved Cas9 variants with broad PAM compatibility and high DNA specificity, Nature, 556, 57, 10.1038/nature26155 · DOI · Paper page
Cited by
Sorted by citations
Items 101–200 of 372
CRISPR/Cas9-mediated precise targeted mutagenesis of phytoene desaturase in celery
Liu, J.-X., Li, T., Wang, H., Liu, Y., Feng, K., Duan, A., Liu, H., Shu, S., & Xiong, A. (2022) · Horticulture Research · doi:10.1093/hr/uhac162 · Cited by 20
Crop genome editing through tissue-culture-independent transformation methods
Sebiani-Calvo, A., Hernández-Soto, A., Hensel, G., & Gatica-Arias, A. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2024.1490295 · Cited by 19
Ahmad, M. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.1002596 · Cited by 19
Naeem, M. S., Zaman, W., Saqib, S., Shahzad, A., Rahman, S. U., & Ahmad, N. (2024) · South African Journal of Botany · doi:10.1016/j.sajb.2024.07.038 · Cited by 23
CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects
Sheri, V., Mohan, H., Jogam, P., Alok, A., Rohela, G. K., & Zhang, B. (2025) · Journal of Cotton Research · doi:10.1186/s42397-024-00206-w · Cited by 19
Critical considerations and computational tools in plant genome editing
Saha, D., Panda, A. K., & Datta, S. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e41135 · Cited by 18
Husaini, A. M., & Sohail, M. F. (2024) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2024.04.015 · Cited by 18
Yang, Q., Liu, J., Yu, Y., Cao, Y., Liu, C., Su, H., Huang, T., Liu, S., Yuan, J., Zhao, Z., et al. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.136912 · Cited by 18
CRISPR-Cas13 in malaria parasite: Diagnosis and prospective gene function identification
Quansah, E., Chen, Y., Yang, S., Wang, J., Sun, D., Zhao, Y., Chen, M., Li, Y., & Zhang, C. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1076947 · Cited by 17
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Ali, E., & Zhang, K. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1086847 · Cited by 17
Targeted Modulation of Chicken Genes In Vitro Using CRISPRa and CRISPRi Toolkit
Chapman, B., Han, J. H., Lee, H. J., Ruud, I., & Kim, T. H. (2023) · Genes · doi:10.3390/genes14040906 · Cited by 17
Crop Improvement: Comparison of Transgenesis and Gene Editing
Permyakova, N. V., & Дейнеко, Е. В. (2024) · Horticulturae · doi:10.3390/horticulturae10010057 · Cited by 17
Efficient tobacco rattle virus‐induced gene editing in tomato mediated by the CRISPR/Cas9 system
Wang, Q., Zhang, D., Dai, Y., & Liu, C. (2024) · Biotechnology Journal · doi:10.1002/biot.202400204 · Cited by 17
Unveiling CRISPR/Cas in rapeseed: Triumphs, trials, and tomorrow
Khan, S. U., Qian, M., Li, S., Fan, Y., Wang, H., Chang, W., Alam, O., Saeed, S., & Lu, K. (2024) · New Crops · doi:10.1016/j.ncrops.2024.100045 · Cited by 17
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HPV-driven cancers: a looming threat and the potential of CRISPR/Cas9 for targeted therapy
Kermanshahi, A. Z., Ebrahimi, F., Taherpoor, A., Eslami, N., & Baghi, H. B. (2025) · Virology Journal · doi:10.1186/s12985-025-02783-x · Cited by 18
Application of CRISPR-Mediated Gene Editing for Crop Improvement
Negi, C., Vasistha, N. K., Singh, D., Vyas, P., & Dhaliwal, H. S. (2022) · Molecular Biotechnology · doi:10.1007/s12033-022-00507-y · Cited by 16
Li, L., Cheng, X., Kong, X., Jia, P., Wang, X., Zhang, L., Zhang, X., Zhang, Y., Zhang, Z., & Zhang, B. (2023) · Plants · doi:10.3390/plants12040732 · Cited by 16
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Pan, X., Peng, R., & Zhang, B. (2024) · Journal of Cotton Research · doi:10.1186/s42397-024-00181-2 · Cited by 16
Environmentally friendly plant essential oil: Liquid gold for human health
Chen, Y., Liu, L., Wang, H., Ma, J., Peng, W., Li, X., Lai, Y., Zhang, B., & Zhang, D. (2021) · Advances in agronomy · doi:10.1016/bs.agron.2021.06.005 · Cited by 17
Diané, A., Mu-u-min, R., & Al-Siddiqi, H. (2025) · Cell and Tissue Research · doi:10.1007/s00441-025-03952-8 · Cited by 16
CRISPR/Cas9 for Heat Stress Tolerance in Rice: A Review
Chakraborty, A., & Wylie, S. J. (2025) · Plant Molecular Biology Reporter · doi:10.1007/s11105-025-01564-8 · Cited by 19
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Won, K., Park, S.-I., Choi, J., Kim, H. H., Kang, B., & Kim, H. (2021) · Horticulturae · doi:10.3390/horticulturae7090317 · Cited by 15
Cheng, C., Kan, J., Li, S., Jiang, C., He, X., Shen, H., Xu, R., Li, B., Feng, Z., & Yang, P. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.1018379 · Cited by 15
Hu, N., Tian, H., Li, Y., Xu, L. M., Li, D., Li, L., Wang, S., Zhang, Y., Shi, X., Huang, B., et al. (2025) · Trends in biotechnology · doi:10.1016/j.tibtech.2025.03.016 · Cited by 15
Degradation of extracellular and membrane proteins in targeted therapy: Status quo and quo vadis
Li, Z., Ma, S., Zhang, S., Ma, Z., Du, L., & Li, M. (2023) · Drug Discovery Today · doi:10.1016/j.drudis.2023.103716 · Cited by 14
Matinvafa, M. A., Makani, S., Parsasharif, N., Zahed, M. A., Movahed, E., & Ghiasvand, S. (2023) · 3 Biotech · doi:10.1007/s13205-023-03786-7 · Cited by 17
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Aghaali, Z., & Naghavi, M. R. (2024) · BMC Plant Biology · doi:10.1186/s12870-024-05412-x · Cited by 15
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Rasool, H. M. H., Chen, Q., Gong, X., & Zhou, J. (2024) · The FASEB Journal · doi:10.1096/fj.202401569r · Cited by 14
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Liu, W., & Zhang, B. (2022) · Functional & Integrative Genomics · doi:10.1007/s10142-022-00916-x · Cited by 14
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Sagarbarria, M. G. S., Caraan, J. A. M., & Layos, A. J. G. (2023) · Transgenic Research · doi:10.1007/s11248-023-00371-9 · Cited by 13
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Rohela, G. K., Saini, P. K., Aziz, D., Rafiq, S., Jogam, P., & Zhang, B. (2024) · Industrial Crops and Products · doi:10.1016/j.indcrop.2024.120097 · Cited by 13
Zhang, N., He, J., Muhammad, A., & Shao, Y. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23105443 · Cited by 13
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Amas, J. C., Thomas, W. J. W., Zhang, Y., Edwards, D., & Batley, J. (2022) · Phytopathology · doi:10.1094/phyto-08-22-0289-fi · Cited by 12
Mao, S., Yang, Z., Liu, Z., Wang, Y., Zeng, Y., Jaffrezic-Renault, N. J., Zhang, Z., Dong, Y., & Guo, Z. (2024) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2024.108860 · Cited by 12
Mu-u-min, R., Diané, A., Allouch, A., & Al-Siddiqi, H. (2025) · Biomedicines · doi:10.3390/biomedicines13020383 · Cited by 14
Wu, F., He, W., Xiao, Y., Wu, Q., Zhang, Q., Zhang, T., Xu, L., & Pang, X. (2025) · Advanced Science · doi:10.1002/advs.202408397 · Cited by 12
Wang, T., Lú, Q., Hui, S., Hu, N., Wei, Y., Li, P., Liu, Y., Zhao, Z., Liu, J., Zhang, B., et al. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.741415 · Cited by 12
Deng, J., Wang, Y., Zhang, S., & Chen, L. (2023) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2023.105790 · Cited by 11
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Arif, M., Ilyas, M., Adnan, M., Kalsoom, R., Ren, M., Xu, R., & Li, L. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.142468 · Cited by 14
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Li, X., Li, R., Yuan, Z., Zhu, Z., Xu, W., Wang, Y., Zhang, D., & Yang, L. (2022) · Analytical Chemistry · doi:10.1021/acs.analchem.2c01837 · Cited by 11
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Nawaz, T., Nelson, D., Saleem, A., Bibi, M., Joshi, N., Fahad, S., Saud, S., Adnan, M., Khan, M. N. R., Hassan, S., et al. (2025) · Wiley eBooks · doi:10.1002/9781394273270.ch17 · Cited by 12
Balaky, S. T. J., Hamid, S. F., Uso, R. S., Khaleel, A. A., & Gharibi, A. (2025) · Microchemical Journal · doi:10.1016/j.microc.2025.114213 · Cited by 10
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Ameen, M., Zafar, A., Javaid, M. M., Zia, M. A., Mahmood, A., Naqve, M., & Bibi, S. (2023) · Springer eBooks · doi:10.1007/978-3-031-37428-9_8 · Cited by 10
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Sang, S., Wang, Y., Yao, G., Ma, T., Sun, X., Zhang, Y., Su, N., Tan, X., Abbas, H. M. K., Ji, S., et al. (2024) · Physiologia Plantarum · doi:10.1111/ppl.14254 · Cited by 9
Applications and Prospects of CRISPR/Cas9 Technology in the Breeding of Major Tropical Crops
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Rachappanavar, V. (2025) · Molecular Biology Reports · doi:10.1007/s11033-025-10285-w · Cited by 9
Yang, F., Wu, Q., Zeng, X., Jiang, Q., Zhang, S., Wang, J., Zhang, Q., Li, F., & Xu, D. (2025) · Microbiology Spectrum · doi:10.1128/spectrum.03235-24 · Cited by 9
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Agu, P. C. (2025) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2025.01.001 · Cited by 9
Wang, Y., Tang, Q., Kang, Y., Wang, X., Zhang, H., & Li, X. (2022) · Cells · doi:10.3390/cells11213471 · Cited by 9
Bulle, M., Sheri, V., Aileni, M., & Zhang, B. (2023) · Plants · doi:10.3390/plants12193448 · Cited by 10
Patil, A. M., Pawar, B. D., Wagh, S. G., Markad, N. R., Bhute, N. K., Shinde, H., Shelake, R. M., Červený, J., & Wagh, R. S. (2024) · Preprints.org · doi:10.20944/preprints202405.0751.v1 · Cited by 9
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Liu, B., Wang, Z., Gu, M., Wang, J., & Tan, J. (2024) · Translational Lung Cancer Research · doi:10.21037/tlcr-24-592 · Cited by 12
Bahadır, S., Abdulla, M. F., Mostafa, K., Kavas, M., Hacıkamiloğlu, M. S., Kurt, O., & Yıldırım, K. (2024) · The Plant Genome · doi:10.1002/tpg2.20506 · Cited by 8
Bagale, S., Enesi, R. O., & Gorim, L. Y. (2025) · Rhizosphere · doi:10.1016/j.rhisph.2025.101105 · Cited by 8
Zhang, J., Zhang, X., Liu, M., Jin, Y., Pai, Q., Wu, X., & Sun, D. (2025) · Plants · doi:10.3390/plants14162477 · Cited by 8
Dweh, T. J., Pattnaik, S., & Sahoo, J. P. (2023) · PubMed Central · Cited by 8
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Angon, P. B., & Habiba, U. (2022) · Current Applied Science and Technology · doi:10.55003/cast.2022.03.23.003 · Cited by 9
Mascarenhas, M. S., Nascimento, F. D. S., Rocha, A. D. J., Ferreira, M. D. S., Oliveira, W. D. D. S., Lino, L. S. M., Mendes, T. A. D. O., Ferreira, C. F., dos Santos-Serejo, J. A., & Amorim, É. P. (2024) · Current Issues in Molecular Biology · doi:10.3390/cimb46100659 · Cited by 9
Shetty, A., Kwas, H., Rajhi, H., Rangareddy, H., & Fryer, J. (2024) · Cureus · doi:10.7759/cureus.71697 · Cited by 8
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Kamel, E. M., Ali, M. A. M., Allam, A. A., Ahmed, N., Abalkhail, A., Alkhayl, F. F. A., & Lamsabhi, A. M. (2025) · Functional & Integrative Genomics · doi:10.1007/s10142-025-01708-9 · Cited by 10
Simoni, S., Fambrini, M., Pugliesi, C., & Rogo, U. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26199294 · Cited by 7
Wang, X., Li, D., Tan, X., Chang-chun, C., Xinyao, Z., Zhan, S., Aiguo, Y., Xiankui, F., & Liu, D. (2023) · Euphytica · doi:10.1007/s10681-023-03196-z · Cited by 8
Establishment of genome‐editing system and assembly of a near‐complete genome in broomcorn millet
Liu, Y., Cheng, Z., Chen, W., Wu, C., Chen, J., & Sui, Y. (2024) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13664 · Cited by 9
Advancing our understanding of root development: Technologies and insights from diverse studies
Coll, N. S., Moreno-Risueno, M. Á., Strader, L. C., Goodnight, A. V., & Sozzani, R. (2024) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiae605 · Cited by 7
Michelotti, V., Urbinati, G., Gentile, A., Lucioli, S., Caboni, E., & Tacconi, G. (2022) · Acta Horticulturae · doi:10.17660/actahortic.2022.1332.15 · Cited by 7
Rai, M. N., Rhodes, B., Jinga, S., Kanchupati, P., Ross, E., Carlson, S. R., & Moose, S. P. (2025) · Plant Methods · doi:10.1186/s13007-025-01365-w · Cited by 6
Harzlı, İ., Mostafa, K., Mutlu, V. A., Shamsi, I. H., & Kavas, M. (2025) · Physiologia Plantarum · doi:10.1111/ppl.70582 · Cited by 6
Artificial Intelligence-Assisted CRISPR/Cas Systems for Targeting Plant Viruses
Iksat, N. N., Madirov, A., Zhanassova, K., & Masalimov, Z. K. (2025) · Genes · doi:10.3390/genes16111258 · Cited by 8
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Bhavanee, K. N. D., Krishnamoorthi, A., Rathva, H. M., Mareguddikar, S. C., Singh, A., Singh, B. P., Nageshwar, & Chittibomma, K. (2024) · Journal of Advances in Biology & Biotechnology · doi:10.9734/jabb/2024/v27i2702 · Cited by 11
Li, P., Zhao, Z., Wang, W., Wang, T., Hu, N., Wei, Y., Sun, Z., Chen, Y., Li, Y., Liu, Q., et al. (2024) · BMC Plant Biology · doi:10.1186/s12870-024-05262-7 · Cited by 6
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El‐Awaad, E., & Merzendorfer, H. (2025) · New plant protection. · doi:10.1002/npp2.26 · Cited by 5
Li, X., Cao, C., Bolanos-Villegas, P. A., Liu, Y., Wang, J., Li, Q., Mao, W., Wang, P., Hou, J., Li, L., et al. (2025) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-025-01607-0 · Cited by 5
Momin, M. S. A., Bhadra, J., Bhunia, D., Sannigrahi, A., & De, N. (2025) · Cells · doi:10.3390/cells14120903 · Cited by 6
Liu, L., Zhang, L., & Lu, L. (2025) · Journal of soil science and plant nutrition · doi:10.1007/s42729-025-02541-6 · Cited by 6
Niu, H., Tan, J., Yan, W., Liu, D., & Yang, L. (2025) · Horticulturae · doi:10.3390/horticulturae11091100 · Cited by 5
Shingaliev, A. S., Rekina, A., Gorbachev, M., Dudnikova, K., & Dudnikov, M. (2025) · Horticulturae · doi:10.3390/horticulturae11111297 · Cited by 5
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He, Y., Li, H., Ju, X., & Gong, B. (2024) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2024.189212 · Cited by 6
Metadata
- Authors
- Zhang, D., Zhang, Z., Unver, T., & Zhang, B.
- Affiliations
- Henan Agricultural University (CN)
- Henan Institute of Science and Technology (CN)
- East Carolina University (US)
- Corresponding author
- Zhang, Dangquan
- Zhang, Zhiyong
- Zhang, Baohong
- Venue
- Journal of Advanced Research
- Publisher
- Elsevier BV
- Published
- 2021-03
- Volume
- 29
- Pages
- 207-221
- ISSN
- 2090-1224 (ISSN-L)
- 2090-1232
- PMC
- PMC8020163
- OpenAlex
- W3094156280
- Cited by
- 373
- References
- 147
- Citations by year
- 2026: 53 · 2025: 95 · 2024: 93 · 2023: 67 · 2022: 46 · 2021: 18
- FWCI
- 3.16
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Advanced Research (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (31172257, 31571600, 31271648)
- Science and Technology Innovation Talents in Universities of Henan Province (114100510008)
- Cotton Incorporated
- National Science Foundation (1658709)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- CRISPR/Cas9, genome editing, gene function, crop improvement, precision breeding, pathogen resistance, abiotic stress tolerance, plant development, secondary metabolism, fiber development, protospacer adjacent motif, off-target effects, Cas proteins, gene knock-in, gene knockout, epigenome editing
- MeSH terms
- Plant Breeding, Gene Editing, Agriculture, Humans, Phenotype, Stress, Physiological, Gene Targeting, Crops, Agricultural, Genome, Plant, Plants, Genetically Modified, Gene Knock-In Techniques, Agrobacterium, Plant Development, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems
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