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CRISPR/Cas: A powerful tool for gene function study and crop improvement
Open accessAbstract
It is a long-standing goal of scientists and breeders to precisely control a gene for studying its function as well as improving crop yield, quality, and tolerance to various environmental stresses. The discovery and modification of CRISPR/Cas system, a nature-occurred gene editing tool, opens an era for studying gene function and precision crop breeding. In this review, we first introduce the brief history of CRISPR/Cas discovery followed the mechanism and application of CRISPR/Cas system on gene function study and crop improvement. Currently, CRISPR/Cas genome editing has been becoming a mature cutting-edge biotechnological tool for crop improvement that already used in many different traits in crops, including pathogen resistance, abiotic tolerance, plant development and morphology and even secondary metabolism and fiber development. Finally, we point out the major issues associating with CRISPR/Cas system and the future research directions. Key Scientific Concepts of Review: CRISPR/Cas9 system is a robust and powerful biotechnological tool for targeting an individual DNA and RNA sequence in the genome. It can be used to target a sequence for gene knockin, knockout and replacement as well as monitoring and regulating gene expression at the genome and epigenome levels by binding a specific sequence. Agrobacterium-mediated method is still the major and efficient method for delivering CRISPR/Cas regents into targeted plant cells. However, other delivery methods, such as virus-mediated method, have been developed and enhanced the application potentials of CRISPR/Cas9-based crop improvement. PAM requirement offers the CRISPR/Cas9-targted genetic loci and also limits the application of CRISPR/Cas9. Discovering new Cas proteins and modifying current Cas enzymes play an important role in CRISPR/Cas9-based genome editing. Developing a better CRISPR/Cas9 system, including the delivery system and the methods eliminating off-target effects, and finding key/master genes for controlling crop growth and development is two major directions for CRISPR/Cas9-based crop improvement.
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![Fig. 2 Application of CRISPR/Cas genome editing in crop improvement, CRISPR/Cas is a robust tool and have huge potentials on crop improvement. Though targeting an individual DNA sequence, CRISPR/Cas can be used to add and/or change the expression of an individual gene that controls plant tolerance to abiotic and biotic stresses as well as plant growth and development. CRISPR/Cas also can change the secondary metabolism for controlling the biosynthesis of protein, carbohydrate, oil, and functional components. Partial figures are modified from previous publications [74], [108].](/paper-figures/6f08ea3b35d948ec97e53b01adce3ae0/crispr-cas-a-powerful-tool-for-gene-function-study-and-crop-improvement-figure-2.jpg)
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“| Delivery methods improvement | Application in vivo [95–105] | AAV: Last for years in terminally differentiated cell types | May cause accumulation of off-target mutations |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [101]
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“| Delivery methods improvement | Application in vivo [95–105] | LNP: Can be quickly degraded in vivo | Eliminate off-target effects |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [101]
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“Designing sgRNA is more convenient, programmable and cost-effective than designing DNA binding domains, thus CRISPR/Cas9 is more favored than ZFNs and TALENs and has revolutionized the biotechnology field (Zhang et al., 2021; Tran et al., 2022).”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Zhang et al., 2021
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“Designing sgRNA is more convenient, programmable and cost-effective than designing DNA binding domains, thus CRISPR/Cas9 is more favored than ZFNs and TALENs and has revolutionized the biotechnology field (Zhang et al., 2021; Tran et al., 2022).”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Zhang et al., 2021
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In the citing paper
“| Delivery methods improvement | Application in vivo [95–105] | AAV: Last for years in terminally differentiated cell types | May cause accumulation of off-target mutations |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [101]
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In the citing paper
“| Delivery methods improvement | Application in vivo [95–105] | LNP: Can be quickly degraded in vivo | Eliminate off-target effects |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [101]
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · cited 2×
“If the system binds to the promoter region, the CRISPR/dCas system will block transcription factor and RNA polymerase (RNAP) binding to the promoter region and inhibit transcription initiation; otherwise, if the CRISPR/dCas system binds to the coding region, it will block RNAP binding and inhibit transcription elongat…”
BackgroundSearch-and-replace genome editing without double-strand breaks or donor DNA
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696 · cited 3×
“At the same time, if a transcription activator binds to the dCas9 protein, the CRISPR/dCas system will enhance the expression of the targeted genes [68].”
BackgroundInfluentialGenetic screens in human cells using the CRISPR-Cas9 system
Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014) · Science · doi:10.1126/science.1246981 · Cited by 2,856
Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769
Research works printed but not linked: 3
- Martyn, 1991, Resistance to races 0, 1, and 2 of Fusarium wilt of watermelon in Citrullus sp, PI-296341 -FR., 26, 429
- Zhang, 2016, Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA, Nat Commun, 7, 12617, 10.1038/ncomms12617 · DOI
- Hu, 2018, Evolved Cas9 variants with broad PAM compatibility and high DNA specificity, Nature, 556, 57, 10.1038/nature26155 · DOI · Paper page
Cited by
Sorted by citations
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Moescheid, M. F., Wisitphongpun, P., Mann, V. H., Quack, T., Grunau, C., Grevelding, C. G., Ittiprasert, W., & Brindley, P. J. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.09.12.557428 · Cited by 2
Ittiprasert, W., Moescheid, M. F., Wisitphongpun, P., Mann, V. H., Quack, T., Grevelding, C. G., Grunau, C., & Brindley, P. J. (2023) · Research Square · doi:10.21203/rs.3.rs-3492058/v1 · Cited by 2
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(2023) · Trends in Animal and Plant Sciences · doi:10.62324/taps/2023.011 · Cited by 1
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Walker-Smith, T. (2024) · Genetics. · doi:10.5772/intechopen.1005595 · Cited by 1
Li, X., Wang, G., Wang, J., Song, X., Xiong, Z., Xia, Y., & Ai, L. (2024) · Foodborne Pathogens and Disease · doi:10.1089/fpd.2024.0028 · Cited by 1
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Nizovtseva, I. G., Chernushkin, D., Rezaykin, A. V., Svitich, V., Korenskaia, A. E., Mikushin, P., & Стародумов, И. О. (2024) · Theoretical Foundations of Chemical Engineering · doi:10.1134/s0040579525600743 · Cited by 1
Sun, K., Liu, S., Mao, H., Zha, Q., Liu, H., Shen, S., Jacobsen, E., Visser, R. G. F., Bai, Y., Li, C., et al. (2025) · Frontiers in Plant Science · doi:10.3389/fpls.2025.1614191 · Cited by 1
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Eman, I., Akram, A., Malik, M., Amjad, K., Hameed, M., Khan, Z., & Shah, T. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-45576-6.00011-x · Cited by 1
Meng, G., Wang, S. H., Hou, Y., Li, W., Yang, S., Ge, T., Song, C., Liu, P., Yang, W., Pang, G., et al. (2026) · Molecular Plant Pathology · doi:10.1111/mpp.70242 · Cited by 1
Advances in gene editing for legume improvement: technologies, progress, and prospects
Thakur, S., Pareek, S., Dixit, G. P., Meru, G., & Das, A. (2026) · Frontiers in Genome Editing · doi:10.3389/fgeed.2026.1789952 · Cited by 1
Das, D., Sachdeva, S., Vats, G., Singh, A. K., Maurya, A., Bansal, S., Singh, A. K., Rajkumar, S., Singh, G. P., & SINGH, R. D. (2026) · Journal of Agriculture and Food Research · doi:10.1016/j.jafr.2026.102935 · Cited by 1
Hailu, G. G., Kumar, A., Khan, I., Chelliah, R., Choi, S., Lee, O., Oh, D. H., Liu, S., & Wei, S. (2026) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.70490 · Cited by 1
Wu, Y., Zhou, S., Ye, X., Zhu, T., Deng, F., & Yang, D. (2026) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2026.118893 · Cited by 1
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Song, S., Fan, X., Zhang, N., Lin, N., & Wang, G. (2026) · Plants · doi:10.3390/plants15132024 · Cited by 1
Fatima, P., Singh, S., Mishra, A., Kumar, R., Singh, A. K., Parveen, S., Kumari, M., Dei, S., & Shamim, M. (2026) · Discover Plants. · doi:10.1007/s44372-026-00744-4 · Cited by 1
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Zhang, M., Liu, Y., Tan, H., Zhao, Z., Zhang, B., Niu, H., & Yang, L. (2026) · Horticulturae · doi:10.3390/horticulturae12081007 · Cited by 1
Bae, S.-H., Oh, J., Kim, E. H., Kim, S. M., Jeong, H.-W., & Shin, J.-S. (2022) · Horticulture Environment and Biotechnology · doi:10.1007/s13580-022-00427-5 · Cited by 1
Louwaars, N. P. (2022) · Wageningen Academic Publishers eBooks · doi:10.3920/978-90-8686-933-6_7 · Cited by 1
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Saeed, F., Shah, T. M., Bukhat, S., Munsif, F., Ahmad, I., Khan, H., & Khan, A. (2022) · Springer eBooks · doi:10.1007/978-3-031-08072-2_1 · Cited by 1
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Goel, N., Arora, A., & Kumar, A. (2024) · Springer eBooks · doi:10.1007/978-981-99-8529-6_14 · Cited by 1
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Sidhic, J., Prakāsh, C., Sarath, N. G., Narayanankutty, A., George, S., & Sebastian, D. P. (2024) · Springer eBooks · doi:10.1007/978-981-97-2166-5_5 · Cited by 1
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Dev, D., Antre, S. H., Nandni, S., Pandey, S., Singh, M. K., & Singh, A. K. (2024) · South African Journal of Botany · doi:10.1016/j.sajb.2024.06.011 · Cited by 1
Rao, J., Wang, X., Chen, X., Liu, Y., Jiang, J., & Wang, Z. (2024) · Gene · doi:10.1016/j.gene.2024.148760 · Cited by 1
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Ribeiro, C. A. M., Costa, M. D. O. C. E., Farias, A. B., Ribeiro, R. D. R., de Lima, Y. F. A., de Souza, N., Chiquitto, A. G., Nichio, B. T. D. L., Paschoal, A. R., Oliveira, L. S., et al. (2024) · Computer-aided drug discovery and design · doi:10.1007/978-3-031-69162-1_2 · Cited by 1
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Rahmati, H., Garmabi, B., Ghazvini, H., Tamijani, S. M. S., Mousavi-Bourkheili, S. M., Sarvandani, M. N., & Rafaiee, R. (2025) · Biological Rhythm Research · doi:10.1080/09291016.2025.2459689 · Cited by 1
Wang, H., Meng, H., Qi, X., Pan, Y., Ji, B., Wen, L., Zan, Y., Si, H., Wang, Y., Liu, D., et al. (2025) · Agronomy · doi:10.3390/agronomy15040884 · Cited by 1
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Su, C.-W., Tsai, L., Hsu, Y.-C., Lu, Y., Jc, L., Linacre, A. M. T., & Hsieh, H. (2025) · Forensic Science Medicine and Pathology · doi:10.1007/s12024-025-01079-4 · Cited by 1
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Zuo, X., Zhao, P., & Ren, Z. (2021) · Molecular Biotechnology · doi:10.1007/s12033-021-00428-2 · Cited by 1
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OLIVEIRA, K. E. S. D., Ribeiro, C. B., Carneiro, A. A., & Carneiro, N. P. (2022) · Revista Brasileira de Milho e Sorgo · doi:10.18512/rbms2022v21e1284
Miccoli, C., Gambacorta, G., Urbinati, G., Santiago-Reyes, M., Gentile, A., Monticelli, S., Caboni, E., Humberto Prieto, E., Verde, I., Decroocq‐Ferrant, V., et al. (2022) · Acta Horticulturae · doi:10.17660/actahortic.2022.1352.58 · Cited by 1
CRISPR/Cas9: An evolutionary approach towards crops amelioration
Kapil, J., Fartyal, M., & Mathur, N. (2023) · LS International Journal of Life Sciences · doi:10.5958/2319-1198.2023.00004.0 · Cited by 1
Baruah, I. K., Baruah, G., Paul, S., Devi, L., Boruah, B., Devi, R. S., Hazarika, M., Saikia, T., & Bhuyan, J. (2023) · Rhizosphere biology · doi:10.1007/978-981-99-0030-5_4 · Cited by 1
Wang, X., Li, D., Tan, X., Cai, C., Zhang, X., Shen, Z., Yang, A., Fu, X., & Liu, D. (2022) · Research Square · doi:10.21203/rs.3.rs-1892545/v1 · Cited by 1
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Mishra, R., Joshi, R. K., & Zhao, K. (2022) · Springer eBooks · doi:10.1007/978-981-19-0600-8_1
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Shyamli, P. S., Suranjika, S., Pradhan, S., & Parida, A. K. (2022) · Springer eBooks · doi:10.1007/978-3-031-08072-2_5
Metadata
- Authors
- Zhang, D., Zhang, Z., Unver, T., & Zhang, B.
- Affiliations
- Henan Agricultural University (CN)
- Henan Institute of Science and Technology (CN)
- East Carolina University (US)
- Corresponding author
- Zhang, Dangquan
- Zhang, Zhiyong
- Zhang, Baohong
- Venue
- Journal of Advanced Research
- Publisher
- Elsevier BV
- Published
- 2021-03
- Volume
- 29
- Pages
- 207-221
- ISSN
- 2090-1224 (ISSN-L)
- 2090-1232
- PMC
- PMC8020163
- OpenAlex
- W3094156280
- Cited by
- 373
- References
- 147
- Citations by year
- 2026: 53 · 2025: 95 · 2024: 93 · 2023: 67 · 2022: 46 · 2021: 18
- FWCI
- 3.16
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Advanced Research (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (31172257, 31571600, 31271648)
- Science and Technology Innovation Talents in Universities of Henan Province (114100510008)
- Cotton Incorporated
- National Science Foundation (1658709)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- CRISPR/Cas9, genome editing, gene function, crop improvement, precision breeding, pathogen resistance, abiotic stress tolerance, plant development, secondary metabolism, fiber development, protospacer adjacent motif, off-target effects, Cas proteins, gene knock-in, gene knockout, epigenome editing
- MeSH terms
- Plant Breeding, Gene Editing, Agriculture, Humans, Phenotype, Stress, Physiological, Gene Targeting, Crops, Agricultural, Genome, Plant, Plants, Genetically Modified, Gene Knock-In Techniques, Agrobacterium, Plant Development, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems
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