CRISPR/Cas: A powerful tool for gene function study and crop improvement

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339

RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · cited 2×

Search-and-replace genome editing without double-strand breaks or donor DNA

Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696 · cited 3×

Genetic screens in human cells using the CRISPR-Cas9 system

Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014) · Science · doi:10.1126/science.1246981 · Cited by 2,856

Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769

In vivo genome editing using Staphylococcus aureus Cas9

Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., et al. (2015) · Nature · doi:10.1038/nature14299 · Cited by 2,687

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Zheng, Z., & Joung, J. K. (2016) · Nature · doi:10.1038/nature16526 · Cited by 2,673

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Haft, D. H., Horvath, P., et al. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0299-x · Cited by 2,445

Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product

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Rationally engineered Cas9 nucleases with improved specificity

Slaymaker, I. M., Gao, L., Zetsche, B., Scott, D. A., Yan, W. X., & Zhang, F. (2016) · Science · doi:10.1126/science.aad5227 · Cited by 2,414

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

Garneau, J. E., Dupuis, M.-È., Villion, M., Romero, D. A., Barrangou, R., Boyaval, P., Fremaux, C., Horvath, P., Magadán, A. H., & Moineau, S. (2010) · Nature · doi:10.1038/nature09523 · Cited by 2,336

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

Abudayyeh, O. O., Gootenberg, J. S., Konermann, S., Joung, J., Slaymaker, I. M., Cox, D. B. T., Shmakov, S., Makarova, K. S., Semenova, E., Minakhin, L., et al. (2016) · Science · doi:10.1126/science.aaf5573 · Cited by 2,336

Crystal structure of Cas9 in complex with guide RNA and target DNA

Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

Wang, Y., Cheng, X., Shan, Q., Zhang, Y., Liu, J., Gao, C., & Qiu, J.-L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2969 · Cited by 2,072

Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements

Mojica, F. J. M., D�ez-Villase�or, C., Garc�a-Mart�nez, J., & Soria, E. (2005) · Journal of Molecular Evolution · doi:10.1007/s00239-004-0046-3 · Cited by 2,042

Identification of genes that are associated with DNA repeats in prokaryotes

Jansen, R., Embden, J. D. A. V., Gaastra, W., & Schouls, L. M. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02839.x · Cited by 1,802

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J.-R. J., et al. (2015) · Nature · doi:10.1038/nature14592 · Cited by 1,624

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Hu, J. H., Miller, S. M., Geurts, M. H., Tang, W., Chen, L., Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., et al. (2018) · Nature · doi:10.1038/nature26155 · Cited by 1,552

The CRISPR tool kit for genome editing and beyond

Adli, M. (2018) · Nature Communications · doi:10.1038/s41467-018-04252-2 · Cited by 1,545

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Gelvin, S. B. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.1.16-37.2003 · Cited by 1,383

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

Anders, C., Niewoehner, O., Duerst, A., & Jinek, M. (2014) · Nature · doi:10.1038/nature13579 · Cited by 1,323

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

Harrington, L. B., Burstein, D., Chen, J. S., Páez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aav4294 · Cited by 1,243

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020) · Science · doi:10.1126/science.aba8853 · Cited by 1,233

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

Woo, J. W., Kim, J., Kwon, S. I., Corvalán, C., Cho, S. W., Kim, H., Kim, S.-G., Kim, S.-T., Choe, S., & Kim, J.-S. (2015) · Nature Biotechnology · doi:10.1038/nbt.3389 · Cited by 1,219

The Barley Mlo Gene: A Novel Control Element of Plant Pathogen Resistance

Büschges, R., Hollricher, K., Panstruga, R., Simons, G., Wolter, M., Frijters, A., van Daelen, R., van der Lee, T., Diergaarde, P. J., Groenendijk, J., et al. (1997) · Cell · doi:10.1016/s0092-8674(00)81912-1 · Cited by 1,194

Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing

Rodríguez-Leal, D., Lemmon, Z. H., Man, J., Bartlett, M. E., & Lippman, Z. B. (2017) · Cell · doi:10.1016/j.cell.2017.08.030 · Cited by 1,180

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

Chen, J. S., Dagdas, Y. S., Kleinstiver, B. P., Welch, M. M., Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J. A. (2017) · Nature · doi:10.1038/nature24268 · Cited by 1,168

Engineered CRISPR-Cas9 nuclease with expanded targeting space

Nishimasu, H., Shi, X., Ishiguro, S., Gao, L., Hirano, S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H., et al. (2018) · Science · doi:10.1126/science.aas9129 · Cited by 1,100

ARGOS 8 variants generated by CRISPR ‐Cas9 improve maize grain yield under field drought stress conditions

Shi, J., Gao, H., Wang, H., Lafitte, H. R., Archibald, R. L., Yang, M., Hakimi, S. M., Mo, H., & Habben, J. E. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12603 · Cited by 1,029

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

Fonfara, I., Richter, H., Bratovič, M., Le Rhun, A., & Charpentier, E. (2016) · Nature · doi:10.1038/nature17945 · Cited by 988

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Liang, Z., Chen, K., Li, T., Zhang, Y., Wang, Y., Zhao, Q., Liu, J., Zhang, H., Liu, C., Ran, Y., et al. (2017) · Nature Communications · doi:10.1038/ncomms14261 · Cited by 987

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Zhang, Y., Liang, Z., Zong, Y., Wang, Y., Liu, J., Chen, K., Qiu, J., & Gao, C. (2016) · Nature Communications · doi:10.1038/ncomms12617 · Cited by 927

Prime genome editing in rice and wheat

Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A. V., Raguram, A., Doman, J. L., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0455-x · Cited by 873 · cited 1×

Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922

Wang, F., Wang, C., Liu, P., Lei, C., Hao, W., Gao, Y., Liu, Y., & Zhao, K. (2016) · PLoS ONE · doi:10.1371/journal.pone.0154027 · Cited by 849

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

Svitashev, S. K., Schwartz, C., Lenderts, B., Young, J. K., & Cigan, A. M. (2016) · Nature Communications · doi:10.1038/ncomms13274 · Cited by 841

Development of broad virus resistance in non‐transgenic cucumber using CRISPR/Cas9 technology

Chandrasekaran, J., Brumin, M., Wolf, D., Leibman, D., Klap, C., Pearlsman, M., Sherman, A., Arazi, T., & Gal‐On, A. (2016) · Molecular Plant Pathology · doi:10.1111/mpp.12375 · Cited by 823

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S. (2017) · Scientific Reports · doi:10.1038/s41598-017-00578-x · Cited by 769

DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins

Malnoy, M., Viola, R., Jung, M., Koo, O. J., Kim, S., Kim, J., Velasco, R., & Kanchiswamy, C. N. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01904 · Cited by 766

Engineering canker‐resistant plants through CRISPR /Cas9‐targeted editing of the susceptibility gene Cs LOB 1 promoter in citrus

Peng, A., Chen, S., Lei, T., Xu, L., He, Y., Liu, W., Yao, L., & Zou, X. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12733 · Cited by 732

In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

Kim, E., Koo, T., Park, S. W., Kim, D., Kim, K., Cho, H.-Y., Song, D. W., Lee, K. J., Jung, M. H., Kim, S., et al. (2017) · Nature Communications · doi:10.1038/ncomms14500 · Cited by 715

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

Hou, Z., Zhang, Y., Propson, N. E., Howden, S. E., Chu, L.-F., Sontheimer, E. J., & Thomson, J. A. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1313587110 · Cited by 681

Os8N3 is a host disease-susceptibility gene for bacterial blight of rice

Yang, B., Sugio, A., & White, F. F. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604088103 · Cited by 650

Low‐gluten, nontransgenic wheat engineered with CRISPR/Cas9

Sánchez‐León, S., Gil‐Humanes, J., Ozuna, C. V., Giménez, M. J., Sousa, C., Voytas, D. F., & Barro, F. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12837 · Cited by 619

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Topkar, V. V., Zheng, Z., & Joung, J. K. (2015) · Nature Biotechnology · doi:10.1038/nbt.3404 · Cited by 611

MicroRNAs and their regulatory roles in animals and plants

Zhang, B., Wang, Q., & Pan, X. (2006) · Journal of Cellular Physiology · doi:10.1002/jcp.20869 · Cited by 555

Plant gene editing through de novo induction of meristems

Maher, M. F., Nasti, R. A., Vollbrecht, M., Starker, C. G., Clark, M. D., & Voytas, D. F. (2020) · Nature Biotechnology · doi:10.1038/s41587-019-0337-2 · Cited by 541

Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

Andersson, M., Turesson, H., Nicolia, A., Fält, A., Samuelsson, M., & Hofvander, P. (2016) · Plant Cell Reports · doi:10.1007/s00299-016-2062-3 · Cited by 540

Directed evolution of CRISPR-Cas9 to increase its specificity

Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y.-H., Lee, K., Jung, I., Kim, D., Kim, S., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05477-x · Cited by 525

Reassessment of the Four Yield-related Genes Gn1a, DEP1, GS3, and IPA1 in Rice Using a CRISPR/Cas9 System

Li, M., Li, X., Zhou, Z., Wu, P., Fang, M., Pan, X., Lin, Q., Luo, W., Wu, G., & Li, H. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.00377 · Cited by 513

MicroRNA: a new target for improving plant tolerance to abiotic stress

Zhang, B. (2015) · Journal of Experimental Botany · doi:10.1093/jxb/erv013 · Cited by 510

A highly specific SpCas9 variant is identified by in vivo screening in yeast

Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin, F., Prandi, D., Romanel, A., Demichelis, F., et al. (2018) · Nature Biotechnology · doi:10.1038/nbt.4066 · Cited by 493

Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes

Sun, Y., Jiao, G., Liu, Z., Zhang, X., Li, J., Guo, X., Du, W., Du, J., Francis, F., Zhao, Y., et al. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00298 · Cited by 484

Genome editing in potato via CRISPR‐Cas9 ribonucleoprotein delivery

Andersson, M., Turesson, H., Olsson, N., Fält, A., Ohlsson, P., González, M. N., Samuelsson, M., & Hofvander, P. (2018) · Physiologia Plantarum · doi:10.1111/ppl.12731 · Cited by 435

Meganucleases and Other Tools for Targeted Genome Engineering: Perspectives and Challenges for Gene Therapy

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Engineering of CRISPR/Cas9‐mediated potyvirus resistance in transgene‐free Arabidopsis plants

Pyott, D. E., Sheehan, E., & Molnár, A. (2016) · Molecular Plant Pathology · doi:10.1111/mpp.12417 · Cited by 417

Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Papikian, A., Liu, W., Gallego‐Bartolomé, J., & Jacobsen, S. E. (2019) · Nature Communications · doi:10.1038/s41467-019-08736-7 · Cited by 415

Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing

Li, X., Wang, Y., Chen, S., Tian, H., Fu, D., Zhu, B., Luo, Y., & Zhu, H. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00559 · Cited by 404

Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains

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Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs

Ellison, E. E., Nagalakshmi, U., Gamo, M. E., Huang, P., Dinesh‐Kumar, S. P., & Voytas, D. F. (2020) · Nature Plants · doi:10.1038/s41477-020-0670-y · Cited by 377

Structure and Engineering of Francisella novicida Cas9

Hirano, H., Gootenberg, J. S., Horii, T., Abudayyeh, O. O., Kimura, M., Hsu, P. D., Nakane, T., Ishitani, R., Hatada, I., Zhang, F., et al. (2016) · Cell · doi:10.1016/j.cell.2016.01.039 · Cited by 369

Tomato facultative parthenocarpy results from Sl AGAMOUS ‐ LIKE 6 loss of function

Klap, C., Yeshayahou, E., Bolger, A., Arazi, T., Gupta, S. K., Shabtai, S., Usadel, B., Salts, Y., & Barg, R. (2016) · Plant Biotechnology Journal · doi:10.1111/pbi.12662 · Cited by 365

CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons

Billon, P., Bryant, E. E., Joseph, S. A., Nambiar, T. S., Hayward, S. B., Rothstein, R., & Ciccia, A. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.08.008 · Cited by 363

Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9

Ma, X., Zhang, X., Liu, H., & Li, Z. (2020) · Nature Plants · doi:10.1038/s41477-020-0704-5 · Cited by 362

Continuous evolution of SpCas9 variants compatible with non-G PAMs

Miller, S. M., Wang, T., Randolph, P. B., Arbab, M., Shen, M. W., Huang, T. P., Matuszek, Ż., Newby, G. A., Rees, H. A., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0412-8 · Cited by 359

Transcription at different salinities of Haloferax mediterranei sequences adjacent to partially modified Pst I sites

Mojica, F. J. M., Juez, G., & Rodriguez‐Valera, F. (1993) · Molecular Microbiology · doi:10.1111/j.1365-2958.1993.tb01721.x · Cited by 349

CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations

Kuscu, C., Parlak, M., Tufan, T., Yang, J., Szlachta, K., Wei, X., Mammadov, R., & Adli, M. (2017) · Nature Methods · doi:10.1038/nmeth.4327 · Cited by 344

Design of a bacterial speck resistant tomato by CRISPR /Cas9‐mediated editing of Sl JAZ 2

Ortigosa, A., Ibanez, S. G., Leonhardt, N., & Solano, R. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.13006 · Cited by 344

Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice

Xu, R., Yang, Y., Qin, R., Li, H., Qiu, C., Li, L., Wei, P., & Yang, J. (2016) · Journal of Genetics and Genomics · doi:10.1016/j.jgg.2016.07.003 · Cited by 332

Engineering abiotic stress tolerance via CRISPR/ Cas-mediated genome editing

Zafar, S. A., Zaidi, S. S., Gaba, Y., Singla-Pareek, S. L., Dhankher, O. P., Li, X., Mansoor, S., & Pareek, A. (2019) · Journal of Experimental Botany · doi:10.1093/jxb/erz476 · Cited by 331

Improved drought stress tolerance in Arabidopsis by CRISPR/dCas9 fusion with a Histone AcetylTransferase

Paixão, J. F. R., Gillet, F., Ribeiro, T. P., Bournaud, C., Lourenço‐Tessutti, I. T., Vasquez, D. D. N., de Melo, B. P., Engler, J. D. A., & Grossi‐de‐Sá, M. F. (2019) · Scientific Reports · doi:10.1038/s41598-019-44571-y · Cited by 318

Long stretches of short tandem repeats are present in the largest replicons of the Archaea Haloferax mediterranei and Haloferax volcanii and could be involved in replicon partitioning

Mojica, F. J. M., Ferrer, C., Juez, G., & Rodríguez‐Valera, F. (1995) · Molecular Microbiology · doi:10.1111/j.1365-2958.1995.mmi_17010085.x · Cited by 307

MicroRNAs in Control of Plant Development

Li, C., & Zhang, B. (2015) · Journal of Cellular Physiology · doi:10.1002/jcp.25125 · Cited by 306

Combined SHOOT MERISTEMLESS and WUSCHEL trigger ectopic organogenesis in Arabidopsis

Gallois, J., Woodward, C., Reddy, G. V., & Sablowski, R. W. M. (2002) · Development · doi:10.1242/dev.129.13.3207 · Cited by 281

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Jiang, Y., Chai, Y., Lu, M., Han, X., Lin, Q., Zhang, Y., Zhang, Q., Zhou, Y., Wang, X., Gao, C., et al. (2020) · Genome biology · doi:10.1186/s13059-020-02170-5 · Cited by 281

The AREB1 Transcription Factor Influences Histone Acetylation to Regulate Drought Responses and Tolerance in Populus trichocarpa

Li, S., Lin, Y. J., Wang, P., Zhang, B., Li, M., Chen, S., Shi, R., Tunlaya‐Anukit, S., Liu, X., Wang, Z., et al. (2018) · The Plant Cell · doi:10.1105/tpc.18.00437 · Cited by 275

CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance

Li, R., Liu, C., Zhao, R., Wang, L., Chen, L., Yu, W., Zhang, S., Sheng, J., & Shen, L. (2019) · BMC Plant Biology · doi:10.1186/s12870-018-1627-4 · Cited by 269

Base-Editing-Mediated Artificial Evolution of OsALS1 In Planta to Develop Novel Herbicide-Tolerant Rice Germplasms

Kuang, Y., Li, S., Ren, B., Yan, F., Spetz, C., Li, X., Zhou, X., & Zhou, H. (2020) · Molecular Plant · doi:10.1016/j.molp.2020.01.010 · Cited by 268

Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System

Wang, M., Lu, Y., Botella, J. R., Mao, Y., Hua, K., & Zhu, J. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.03.002 · Cited by 262

Manipulating the Biosynthesis of Bioactive Compound Alkaloids for Next-Generation Metabolic Engineering in Opium Poppy Using CRISPR-Cas 9 Genome Editing Technology

Alagoz, Y., Gurkok, T., Zhang, B., & Ünver, T. (2016) · Scientific Reports · doi:10.1038/srep30910 · Cited by 255

Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

Zhai, Y., Yu, K., Cai, S., Hu, L., Amoo, O., Xu, L., Yang, Y., Ma, B., Jiao, Y., Zhang, C., et al. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13281 · Cited by 237

Targeted, efficient sequence insertion and replacement in rice

Lu, Y., Tian, Y., Shen, R., Yao, Q., Wang, M., Chen, M., Dong, J., Zhang, T., Li, F., Lei, M., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0581-5 · Cited by 225

Down Regulation and Loss of Auxin Response Factor 4 Function Using CRISPR/Cas9 Alters Plant Growth, Stomatal Function and Improves Tomato Tolerance to Salinity and Osmotic Stress

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