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Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth: A new approach for drug delivery

Pascucci, Luisa, Coccè, Valentina, Bonomi, Arianna, Ami, Diletta, Ceccarelli, Piero, Ciusani, Emilio, Viganò, Lucia, Locatelli, Alberta, Sisto, Francesca, Doglia, Silvia Maria, Parati, Eugenio, Bernardo, Maria Ester, Muraca, Maurizio, Alessandri, Giulio, Bondiolotti, Gianpietro, Pessina, Augusto·2014·Journal of Controlled Release
DOI 10.1016/j.jconrel.2014.07.042
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Abstract

Mesenchymal stromal cells (MSCs) have been proposed for delivering anticancer agents because of their ability to home in on tumor microenvironment. We found that MSCs can acquire strong anti-tumor activity after priming with Paclitaxel (PTX) through their capacity to uptake and then release the drug. Because MSCs secrete a high amount of membrane microvesicles (MVs), we here investigated the role of MVs in the releasing mechanism of PTX. The murine SR4987 line was used as MSC model. The release of PTX from SR4987 in the conditioned medium (CM) was checked by HPLC and the anti-tumor activity of both CM and MVs was tested on the human pancreatic cell line CFPAC-1. MVs were isolated by ultracentrifugation, analyzed by transmission (TEM) and scanning electron microscopy (SEM), and the presence of PTX by the Fourier transformed infrared (FTIR) microspectroscopy. SR4987 loaded with PTX (SR4987PTX) secreted a significant amount of PTX and their CM possessed strong anti-proliferative activity on CFPAC-1. At TEM and SEM, SR4987PTX showed an increased number of "vacuole-like" structures and shed a relevant number of MVs, but did not differ from untreated SR4987. However, SR4987PTX-derived-MVs (SR4987PTX-MVs) demonstrated a strong anti proliferative activity on CFPAC-1. FTIR analysis of SR4987PTX-MVs showed the presence of an absorption spectrum in the corresponding regions of the PTX marker, absent in MVs from SR4987. Our work is the first demonstration that MSCs are able to package an…

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Research works printed but not linked: 46

  1. Loebinger, 2010, Stem cells as vectors for antitumour therapy, Thorax, 65, 362, 10.1136/thx.2009.128025 · DOI · Paper page
  2. Shah, 2012, Mesenchymal stem cells engineered for cancer therapy, Adv. Drug Deliv. Rev., 64, 739, 10.1016/j.addr.2011.06.010 · DOI
  3. Gjorgieva, 2013, Mesenchymal stem cells for anti-cancer drug delivery, Recent Pat. Anticancer Drug Discov., 8, 310, 10.2174/15748928113089990040 · DOI
  4. Ebrahimi, 2013, Drug delivery using genetically modified mesenchymal stem cells: a promising targeted-delivery method, Hygeia J. Drugs Med., 5, 90
  5. Pessina, 2011, Mesenchymal stromal cells primed with paclitaxel provide a new approach for cancer therapy, PLoS One, 6, e28321, 10.1371/journal.pone.0028321 · DOI
  6. Pessina, 2013, Mesenchymal stromal cells primed with Paclitaxel attract and kill leukaemia cells, inhibit angiogenesis and improve survival of leukaemia-bearing mice, Br. J. Haematol., 160, 766, 10.1111/bjh.12196 · DOI
  7. Bonomi, 2013, Adipose tissue-derived stromal cells primed in vitro with paclitaxel acquire anti-tumor activity, Int. J. Immunopathol. Pharmacol., 26, 33, 10.1177/03946320130260S105 · DOI
  8. Pessina, 2013, Human skin-derived fibroblasts acquire in vitro anti-tumor potential after priming with Paclitaxel, Anticancer Agents Med. Chem., 13, 523
  9. Scanu, 2008, Stimulated T cells generate microparticles, which mimic cellular contact activation of human monocytes: differential regulation of pro- and anti-inflammatory cytokine production by high-density lipoproteins, J. Leukoc. Biol., 83, 921, 10.1189/jlb.0807551 · DOI
  10. Hugel, 2005, Membrane microparticles: two sides of the coin, Physiology, 20, 22, 10.1152/physiol.00029.2004 · DOI · Paper page
  11. Bruno, 2009, Mesenchymal stem cell-derived microvesicles protect against acute tubular injury, J. Am. Soc. Nephrol., 20, 1053, 10.1681/ASN.2008070798 · DOI
  12. van Dommelen, 2012, Microvesicles and exosomes: opportunities for cell-derived membrane vesicles in drug delivery, J. Control. Release, 161, 635, 10.1016/j.jconrel.2011.11.021 · DOI
  13. Rameshwar, 2013, Mesenchymal stem cells can be a conduit for the delivery of therapeutic MicroRNA, J. Stem Cell Res. Ther., 3, e112, 10.4172/2157-7633.1000e112 · DOI
  14. Kim, 2013, Mesenchymal stem cell-derived secretome and microvesicles as a cell-free therapeutics for neurodegenerative disorders, Tissue Eng. Regen. Med., 10, 93, 10.1007/s13770-013-0010-7 · DOI
  15. Tetta, 2011, The role of microvesicles in tissue repair, Organogenesis, 7, 105, 10.4161/org.7.2.15782 · DOI
  16. Bruno, 2013, Microvesicles derived from human bone marrow mesenchymal stem cells inhibit tumor growth, Stem Cells Dev., 22, 758, 10.1089/scd.2012.0304 · DOI
  17. Roccaro, 2013, BM mesenchymal stromal cell-derived exosomes facilitate multiple myeloma progression, J. Clin. Invest., 123, 1542, 10.1172/JCI66517 · DOI
  18. Hoffman, 2013, Stromal-cell and cancer-cell exosomes leading the metastatic exodus for the promised niche, Breast Cancer Res., 15, 310, 10.1186/bcr3426 · DOI
  19. Shen, 2011, Protein targeting to exosomes/microvesicles by plasma membrane anchors, J. Biol. Chem., 286, 14383, 10.1074/jbc.M110.208660 · DOI
  20. McIntosh, 1988, Pancreatic adenocarcinoma in a patient with cystic fibrosis, Am. J. Med., 85, 592, 10.1016/S0002-9343(88)80116-5 · DOI
  21. Schoumacher, 1990, A cystic fibrosis pancreatic adenocarcinoma cell line, Proc. Natl. Acad. Sci. U. S. A., 87, 4012, 10.1073/pnas.87.10.4012 · DOI
  22. Pessina, 1992, Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells, Cytotechnology, 8, 93, 10.1007/BF02525491 · DOI
  23. Pessina, 1997, Expression of B cell markers on SR-4987 cells derived from murine marrow stroma, Exp. Hematol., 25, 536
  24. Pessina, 2011, Mesenchymal stromal cell line resistant to paclitaxel that spontaneously differentiates into osteoblast-like cells, Cell Biol. Toxicol., 27, 169, 10.1007/s10565-010-9179-x · DOI
  25. Mossman, 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods, 65, 55, 10.1016/0022-1759(83)90303-4 · DOI · Paper page
  26. Gianni, 1995, Nonlinear pharmacokinetic of paclitaxel and its pharmacokinetic/pharmacodynamic relationships in humans, J. Clin. Oncol., 13, 180, 10.1200/JCO.1995.13.1.180 · DOI
  27. Lowry, 1951, Protein measurement with folin phenol reagent, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 · DOI
  28. Reed, 1938, A simple method of estimating fifty percent endpoints, Am. J. Hyg., 27, 493
  29. Natalello, 2011, A major role for side-chain polyglutamine hydrogen bonding in irreversible Ataxin-3 aggregation, PLoS One, 6, e18789, 10.1371/journal.pone.0018789 · DOI
  30. Goormaghtigh, 1999, Attenuated total reflection infrared spectroscopy of proteins and lipids in biological membranes, Biochim. Biophys. Acta, 1422, 105, 10.1016/S0304-4157(99)00004-0 · DOI
  31. Kumar, 1995, Comparative in vitro cytotoxic effects of taxol and its major human metabolite 6 alphahydroxytaxol, Cancer Chemother. Pharmacol., 36, 129, 10.1007/BF00689197 · DOI
  32. Yvon, 1999, Taxol suppresses dynamics of individual microtubules in living human tumor cells, Mol. Biol. Cell, 10, 947, 10.1091/mbc.10.4.947 · DOI
  33. Ami, 2012, Fourier transform infrared microspectroscopy of complex biological systems: from intact cells to whole organisms, Methods Mol. Biol., 895, 85, 10.1007/978-1-61779-927-3_7 · DOI
  34. Tamm, 1997, Infrared spectroscopy of proteins and peptides in lipid bilayers, Q. Rev. Biophys., 30, 365, 10.1017/S0033583597003375 · DOI
  35. Weaver, 2005, Decoding the links between mitosis, cancer, and chemotherapy: the mitotic checkpoint, adaptation, and cell death, Cancer Cell, 8, 7, 10.1016/j.ccr.2005.06.011 · DOI
  36. Wang, 2014, A cell-based pharmacokinetics assay for evaluating tubulin-binding drugs, Int. J. Med. Sci., 11, 479, 10.7150/ijms.8340 · DOI
  37. Veldhoen, 2013, The chemotherapeutic agent paclitaxel inhibits autophagy through two distinct mechanisms that regulate apoptosis, Oncogene, 32, 736, 10.1038/onc.2012.92 · DOI
  38. Palade, 1956, Liver microsomes; an integrated morphological and biochemical study, J. Biophys. Biochem. Cytol., 25, 171, 10.1083/jcb.2.2.171 · DOI
  39. Fang, 2007, Higher-order oligomerization targets plasma membrane proteins and HIV gag to exosomes, PLoS Biol., 5, e158, 10.1371/journal.pbio.0050158 · DOI
  40. Hsu, 2003, Exosomes as a tumor vaccine: enhancing potency through direct loading of antigenic peptides, J. Immunother., 26, 440, 10.1097/00002371-200309000-00007 · DOI
  41. Qazi, 2009, Antigen-loaded exosomes alone induce Th1-type memory through a B-cell-dependent mechanism, Blood, 113, 2673, 10.1182/blood-2008-04-153536 · DOI
  42. Janowska-Wieczorek, 2005, Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer, Int. J. Cancer, 113, 752, 10.1002/ijc.20657 · DOI
  43. Hulsmans, 2013, MicroRNA-containing microvesicles regulating inflammation in association with atherosclerotic disease, Cardiovasc. Res., 10.1093/cvr/cvt161 · DOI
  44. Jang, 2013, Exosome derived from epigallocatechin gallate treated breast cancer cells suppresses tumor growth by inhibiting tumor-associated macrophage infiltration and M2 polarization, BMC Cancer, 13, 421, 10.1186/1471-2407-13-421 · DOI
  45. Skog, 2008, Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers, Nat. Cell Biol., 10, 1470, 10.1038/ncb1800 · DOI · Paper page
  46. Rabinowits, 2009, Exosomal microRNA: a diagnostic marker for lung cancer, Clin. Lung Cancer, 10, 42, 10.3816/CLC.2009.n.006 · DOI

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Metadata

Authors
Pascucci, L., Coccè, V., Bonomi, A., Ami, D., Ceccarelli, P., Ciusani, E., Viganò, L., Locatelli, A., Sisto, F., Doglia, S. M., Parati, E., Bernardo, M. E., Muraca, M., Alessandri, G., Bondiolotti, G., & Pessina, A.
Venue
Journal of Controlled Release
Publisher
Elsevier BV
Published
2014-10
Volume
192
Pages
262-270
ISSN
0168-3659
DOI
10.1016/j.jconrel.2014.07.042
Cited by
846
References
6
Language
en
Licence
Funders
AIRC (Associazione Italiana per la Ricerca sul Cancro) (IG-14574)

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CoChat. (2026). Citation check: Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth: A new approach for drug delivery. https://app.cochat.ai/papers/10.1016/j.jconrel.2014.07.042
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Pascucci, L., Coccè, V., Bonomi, A., Ami, D., Ceccarelli, P., Ciusani, E., Viganò, L., Locatelli, A., Sisto, F., Doglia, S. M., Parati, E., Bernardo, M. E., Muraca, M., Alessandri, G., Bondiolotti, G., & Pessina, A. (2014). Paclitaxel is incorporated by mesenchymal stromal cells and released in exosomes that inhibit in vitro tumor growth: A new approach for drug delivery. Journal of Controlled Release. https://doi.org/10.1016/j.jconrel.2014.07.042