siRNA-lipid nanoparticles with long-term storage stability facilitate potent gene-silencing in vivo

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456 · see citing works

A new concept for macromolecular therapeutics in cancer chemotherapy: mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs.

Matsumura, Y., & Maeda, H. (1986) · PubMed · Cited by 6,771 · see citing works

Knocking down barriers: advances in siRNA delivery

Whitehead, K. A., Langer, R., & Anderson, D. G. (2009) · Nature Reviews Drug Discovery · doi:10.1038/nrd2742 · Cited by 3,115 · see citing works

Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs

Soutschek, J., Akinc, A., Bramlage, B., Charissé, K., Constien, R., Donoghue, M., Elbashir, S. M., Geick, A., Hadwiger, P., Harborth, J., et al. (2004) · Nature · doi:10.1038/nature03121 · Cited by 2,189 · see citing works

Oligonucleotide-Modified Gold Nanoparticles for Intracellular Gene Regulation

Rosi, N. L., Giljohann, D. A., Thaxton, C. S., Lytton‐Jean, A. K. R., Han, M. S., & Mirkin, C. A. (2006) · Science · doi:10.1126/science.1125559 · Cited by 1,988 · see citing works

Rational design of cationic lipids for siRNA delivery

Semple, S. C., Akinc, A., Chen, J., Sandhu, A. P., Mui, B. L., Cho, C. K., Sah, D. W. Y., Stebbing, D., Crosley, E. J., Yaworski, E., et al. (2010) · Nature Biotechnology · doi:10.1038/nbt.1602 · Cited by 1,822 · see citing works

Maximizing the potency of siRNA lipid nanoparticles for hepatic gene silencing in vivo

Jayaraman, M., Ansell, S. M., Mui, B. L., Tam, Y. K., Chen, J., Du, X., Butler, D., Eltepu, L., Matsuda, S., Narayanannair, J. K., et al. (2012) · Angewandte Chemie International Edition · doi:10.1002/anie.201203263 · Cited by 1,267 · see citing works

Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms

Akinc, A., Querbes, W., De, S., Qin, J., Frank-Kamenetsky, M., Jayaprakash, K. N., Jayaraman, M., Rajeev, K. G., Cantley, W. L., Dorkin, J. R., et al. (2010) · Molecular Therapy · doi:10.1038/mt.2010.85 · Cited by 1,261 · see citing works

A combinatorial library of lipid-like materials for delivery of RNAi therapeutics

Akinc, A., Zumbuehl, A., Goldberg, M. S., Leshchiner, E. S., Busini, V., Hossain, N., Bacallado, S., Nguyen, D. N., Fuller, J. E., Alvarez, R., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1402 · Cited by 1,228 · see citing works

Multivalent <i>N</i> -Acetylgalactosamine-Conjugated siRNA Localizes in Hepatocytes and Elicits Robust RNAi-Mediated Gene Silencing

Nair, J. K., Willoughby, J. L. S., Chan, A., Charisse, K., Alam, M. R., Wang, Q., Hoekstra, M., Kandasamy, P., Kel’in, A. V., Milstein, S., et al. (2014) · Journal of the American Chemical Society · doi:10.1021/ja505986a · Cited by 1,141 · see citing works

Safety and efficacy of RNAi therapy for transthyretin amyloidosis

Coelho, T., Adams, D., Silva, A., Lozeron, P., Hawkins, P. N., Mant, T., Perez, J., Chiesa, J., Warrington, S., Tranter, E., et al. (2013) · New England Journal of Medicine · doi:10.1056/nejmoa1208760 · Cited by 971 · see citing works

Engineering targeted viral vectors for gene therapy

Waehler, R., Russell, S. J., & Curiel, D. T. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2141 · Cited by 687 · see citing works

In vivo delivery of miRNAs for cancer therapy: Challenges and strategies

Chen, Y., Gao, D.-Y., & Huang, L. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.05.009 · Cited by 681 · see citing works

Dynamic PolyConjugates for targeted in vivo delivery of siRNA to hepatocytes

Rozema, D. B., Lewis, D. L., Wakefield, D. H., Wong, S. C., Klein, J. J., Roesch, P. L., Bertin, S. L., Reppen, T. W., Chu, Q., Blokhin, A. V., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0703778104 · Cited by 658 · see citing works

Biodegradable Lipids Enabling Rapidly Eliminated Lipid Nanoparticles for Systemic Delivery of RNAi Therapeutics

Maier, M. A., Jayaraman, M., Matsuda, S., Liu, J., Barros, S., Querbes, W., Tam, Y. K., Ansell, S. M., Kumar, V., Qin, J., et al. (2013) · Molecular Therapy · doi:10.1038/mt.2013.124 · Cited by 625 · see citing works

Degradable lipid nanoparticles with predictable in vivo siRNA delivery activity

Whitehead, K. A., Dorkin, J. R., Vegas, A. J., Chang, P. H., Veiseh, O., Matthews, J., Fenton, O. S., Zhang, Y., Olejnik, K. T., Yesilyurt, V., et al. (2014) · Nature Communications · doi:10.1038/ncomms5277 · Cited by 615 · see citing works

Effect of an RNA interference drug on the synthesis of proprotein convertase subtilisin/kexin type 9 (PCSK9) and the concentration of serum LDL cholesterol in healthy volunteers: a randomised, single-blind, placebo-controlled, phase 1 trial

Fitzgerald, K. J., Frank-Kamenetsky, M. D., Shulga‐Morskaya, S., Liebow, A., Bettencourt, B. R., Sutherland, J. E., Hutabarat, R. M., Clausen, V. A., Karsten, V., Cehelsky, J. E., et al. (2013) · The Lancet · doi:10.1016/s0140-6736(13)61914-5 · Cited by 574 · see citing works

Cationic lipid saturation influences intracellular delivery of encapsulated nucleic acids

Heyes, J., Palmer, L., Bremner, K., & MacLachlan, I. (2005) · Journal of Controlled Release · doi:10.1016/j.jconrel.2005.06.014 · Cited by 572 · see citing works

Influence of Polyethylene Glycol Lipid Desorption Rates on Pharmacokinetics and Pharmacodynamics of siRNA Lipid Nanoparticles

Mui, B. L., Tam, Y. K., Jayaraman, M., Ansell, S. M., Du, X., Tam, Y. Y. C., Lin, P. J. C., Chen, S., Narayanannair, J. K., Rajeev, K. G., et al. (2013) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2013.66 · Cited by 509 · see citing works

Preclinical and clinical development of siRNA-based therapeutics

Özcan, G., Özpolat, B., Coleman, R. L., Sood, A. K., & Lopez‐Berestein, G. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.01.007 · Cited by 451 · see citing works

Lipid Nanoparticles Containing siRNA Synthesized by Microfluidic Mixing Exhibit an Electron-Dense Nanostructured Core

Leung, A. K. K., Hafez, I. M., Baoukina, S., Belliveau, N. M., Zhigaltsev, I. V., Afshinmanesh, E., Tieleman, D. P., Hansen, C. L., Hope, M. J., & Cullis, P. R. (2012) · The Journal of Physical Chemistry C · doi:10.1021/jp303267y · Cited by 364 · see citing works

Enhancing tumor cell response to chemotherapy through nanoparticle-mediated codelivery of siRNA and cisplatin prodrug

Xu, X., Xie, K., Zhang, X.-Q., Pridgen, E. M., Park, G. Y., Cui, D. S., Shi, J., Wu, J., Kantoff, P. W., Lippard, S. J., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1303958110 · Cited by 340 · see citing works

A pH-sensitive cationic lipid facilitates the delivery of liposomal siRNA and gene silencing activity in vitro and in vivo

Sato, Y., Hatakeyama, H., Sakurai, Y., Hyodo, M., Akita, H., & Harashima, H. (2012) · Journal of Controlled Release · doi:10.1016/j.jconrel.2012.09.009 · Cited by 310 · see citing works

The size of endothelial fenestrae in human liver sinusoids: implications for hepatocyte-directed gene transfer

Wisse, E., Jacobs, F., Topal, B., Frederik, P. M., & Geest, B. D. (2008) · Gene Therapy · doi:10.1038/gt.2008.60 · Cited by 290 · see citing works

Delivery of therapeutic oligonucleotides with cell penetrating peptides

Boisguérin, P., Deshayes, S., Gait, M. J., O’Donovan, L., Godfrey, C., Betts, C., Wood, M. J. A., & Lebleu, B. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.02.008 · Cited by 245 · see citing works

Systematic evaluation of antibody-mediated siRNA delivery using an industrial platform of THIOMAB–siRNA conjugates

Cuellar, T. L., Barnes, D., Nelson, C., Tanguay, J., Yu, S., Wen, X., Scales, S. J., Gesch, J., Davis, D., Smith, A. V. B., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku1362 · Cited by 215 · see citing works

Safety profile of RNAi nanomedicines

Barros, S. A., & Gollob, J. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.06.007 · Cited by 126 · see citing works

Ionization Behavior of Amino Lipids for siRNA Delivery: Determination of Ionization Constants, SAR, and the Impact of Lipid pKa on Cationic Lipid−Biomembrane Interactions

Zhang, J., Fan, H., Levorse, D. A., & Crocker, L. S. (2011) · Langmuir · doi:10.1021/la104590k · Cited by 95 · see citing works

Intra- and Intermonolayer Hydrogen Bonding in Amide-Functionalized Alkanethiol Self-Assembled Monolayers on Gold Nanoparticles

Boal, A. K., & Rotello, V. M. (2000) · Langmuir · doi:10.1021/la0012384 · Cited by 90 · see citing works

Cell-penetrating peptide-conjugated lipid nanoparticles for siRNA delivery

Asai, T., Tsuzuku, T., Takahashi, S., Okamoto, A., Dewa, T., Nango, M., Hyodo, K., Ishihara, H., Kikuchi, H., & Oku, N. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.01.107 · Cited by 84 · see citing works

Stabilization of Ostwald Ripening in Low Molecular Weight Amino Lipid Nanoparticles for Systemic Delivery of siRNA Therapeutics

Gindy, M. E., Feuston, B., Glass, A., Arrington, L., Haas, R. M., Schariter, J., & Stirdivant, S. M. (2014) · Molecular Pharmaceutics · doi:10.1021/mp500367k · Cited by 70 · see citing works

Size-Dependent Knockdown Potential of siRNA-Loaded Cationic Nanohydrogel Particles

Nuhn, L., Tomcin, S., Miyata, K., Mailänder, V., Landfester, K., Kataoka, K., & Zentel, R. (2014) · Biomacromolecules · doi:10.1021/bm501148y · Cited by 68 · see citing works

A window onto siRNA delivery

Wang, Y., & Huang, L. (2013) · Nature Biotechnology · doi:10.1038/nbt.2634 · Cited by 62 · see citing works

Microfluidic-Based Manufacture of siRNA-Lipid Nanoparticles for Therapeutic Applications

Walsh, C., Ou, K., Belliveau, N. M., Leaver, T., Wild, A., Huft, J., Lin, P. J. C., Chen, S., Leung, A. K. K., Lee, J. B., et al. (2014) · Methods in molecular biology · doi:10.1007/978-1-4939-0363-4_6 · Cited by 55 · see citing works

Use of Self-Delivery siRNAs to Inhibit Gene Expression in an Organotypic Pachyonychia Congenita Model

Hickerson, R. P., Flores, M. A., Leake, D., Lara, M. F., Contag, C. H., Leachman, S. A., & Kaspar, R. L. (2011) · Journal of Investigative Dermatology · doi:10.1038/jid.2010.426 · Cited by 54 · see citing works

Interaction of Cholesterol-Conjugated Ionizable Amino Lipids with Biomembranes: Lipid Polymorphism, Structure–Activity Relationship, and Implications for siRNA Delivery

Zhang, J., Fan, H., Levorse, D. A., & Crocker, L. S. (2011) · Langmuir · doi:10.1021/la201464k · Cited by 48 · see citing works

Lipid Nanoparticles Improve Activity of Single-Stranded siRNA and Gapmer Antisense Oligonucleotides in Animals

Prakash, T. P., Lima, W. F., Murray, H. M., Elbashir, S. M., Cantley, W. L., Foster, D., Jayaraman, M., Chappell, A. E., Manoharan, M., Swayze, E. E., et al. (2013) · ACS Chemical Biology · doi:10.1021/cb4001316 · Cited by 48 · see citing works

Challenges in the pharmaceutical development of lipid-based short interfering ribonucleic acid therapeutics

Gindy, M. E., Leone, A. M., & Cunningham, J. J. (2012) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2012.642363 · Cited by 43 · see citing works

Targeted siRNA Delivery and mRNA Knockdown Mediated by Bispecific Digoxigenin-binding Antibodies

Schneider, B., Grote, M., John, M., Haas, A. K., Bramlage, B., lckenstein, L. M., Jahn‐Hofmann, K., Bauss, F., Cheng, W., Croasdale, R., et al. (2012) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2012.39 · Cited by 42 · see citing works

Neutral biodegradable lipid-envelope-type nanoparticle using vitamin A-Scaffold for nuclear targeting of plasmid DNA

Tanaka, H., Akita, H., Ishiba, R., Tange, K., Arai, M., Kubo, K., & Harashima, H. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.016 · Cited by 37 · see citing works

Effect of Functional Group on the Monolayer Structures of Biodegradable Quaternary Ammonium Surfactants

Ge, A., Peng, Q., Wu, H., Liu, H., Tong, Y., Nishida, T., Yoshida, N., Suzuki, K., Sakai, T., Osawa, M., et al. (2013) · Langmuir · doi:10.1021/la403502k · Cited by 19 · see citing works

mRNA-lipid nanoparticle COVID-19 vaccines: structure and stability

Schoenmaker, L., Witzigmann, D., Kulkarni, J. A., Verbeke, R., Kersten, G., Jiskoot, W., & Crommelin, D. J. A. (2021) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2021.120586 · Cited by 1,566 · see citing works

Lipids and Lipid Derivatives for RNA Delivery

Zhang, Y., Sun, C., Wang, C., Jankovic, K. E., & Dong, Y. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00244 · Cited by 638 · see citing works

Challenges of Storage and Stability of mRNA-Based COVID-19 Vaccines

Uddin, M. N., & Roni, M. A. (2021) · Vaccines · doi:10.3390/vaccines9091033 · Cited by 371 · see citing works

Difference in the lipid nanoparticle technology employed in three approved siRNA (Patisiran) and mRNA (COVID-19 vaccine) drugs

Suzuki, Y., & Ishihara, H. (2021) · Drug Metabolism and Pharmacokinetics · doi:10.1016/j.dmpk.2021.100424 · Cited by 304 · see citing works

Lipid nanoparticles for delivery of messenger RNA to the back of the eye

Patel, S., Ryals, R. C., Weller, K. K., Pennesi, M. E., & Sahay, G. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.04.015 · Cited by 260 · see citing works

Advances in structural design of lipid-based nanoparticle carriers for delivery of macromolecular drugs, phytochemicals and anti-tumor agents

Angelova, A., Garamus, V. M., Angelov, B., Tian, Z., Li, Y., & Zou, A. (2017) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2017.04.006 · Cited by 211 · see citing works

Understanding structure-activity relationships of pH-sensitive cationic lipids facilitates the rational identification of promising lipid nanoparticles for delivering siRNAs in vivo

Sato, Y., Hashiba, K., Sasaki, K., Maeki, M., Tokeshi, M., & Harashima, H. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.01.001 · Cited by 180 · see citing works

Research Advances on the Stability of mRNA Vaccines

Cheng, F., Wang, Y., Bai, Y., Liang, Z., Mao, Q., Liu, D., Wu, X., & Xu, M. (2023) · Viruses · doi:10.3390/v15030668 · Cited by 171 · see citing works

Nucleic acid degradation as barrier to gene delivery: a guide to understand and overcome nuclease activity

Zhang, H., Vandesompele, J., Braeckmans, K., Smedt, S. C. D., & Remaut, K. (2023) · Chemical Society Reviews · doi:10.1039/d3cs00194f · Cited by 140 · see citing works

Biodegradable Cationic and Ionizable Cationic Lipids: A Roadmap for Safer Pharmaceutical Excipients

Jörgensen, A. M., Wibel, R., & Bernkop‐Schnürch, A. (2023) · Small · doi:10.1002/smll.202206968 · Cited by 127 · see citing works

Hyaluronic acid coated PLGA nanoparticulate docetaxel effectively targets and suppresses orthotopic human lung cancer

Wu, J., Deng, C., Meng, F., Zhang, J., Sun, H., & Zhong, Z. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.12.024 · Cited by 107 · see citing works

A flexible, thermostable nanostructured lipid carrier platform for RNA vaccine delivery

Gerhardt, A., Voigt, E. A., Archer, M. C., Reed, S., Larson, E., Hoeven, N. V., Kramer, R. M., Fox, C. B., & Casper, C. (2022) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2022.03.009 · Cited by 86 · see citing works

An imidazole modified lipid confers enhanced mRNA-LNP stability and strong immunization properties in mice and non-human primates

Ripoll, M., Bernard, M., Vaure, C., Bazin, E., Commandeur, S., Perkov, V., Lemdani, K., Nicolaï, M., Bonifassi, P., Kichler, A., et al. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121570 · Cited by 85 · see citing works

Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis

Sun, P., Huang, W., Jin, M., Wang, Q., Fan, B., Kang, L., & Gao, Z. (2016) · International Journal of Nanomedicine · doi:10.2147/ijn.s105427 · Cited by 84 · see citing works

Design and lyophilization of lipid nanoparticles for mRNA vaccine and its robust immune response in mice and nonhuman primates

Suzuki, Y., Miyazaki, T., Muto, H., Kubara, K., Mukai, Y., Watari, R., Sato, S., Kondo, K., Tsukumo, S., Yasutomo, K., et al. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.09.017 · Cited by 81 · see citing works

Therapeutic Potential of LNP-Mediated Delivery of miR-634 for Cancer Therapy

Gokita, K., Inoue, J., Ishihara, H., Kojima, K., & Inazawa, J. (2019) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.10.045 · Cited by 78 · see citing works

Structural analysis of nanoparticulate carriers for encapsulation of macromolecular drugs

Angelov, B., Garamus, V. M., Drechsler, M., & Angelova, A. (2016) · Journal of Molecular Liquids · doi:10.1016/j.molliq.2016.11.064 · Cited by 68 · see citing works

pPB Peptide-Mediated siRNA-Loaded Stable Nucleic Acid Lipid Nanoparticles on Targeting Therapy of Hepatic Fibrosis

Jia, Z., Gong, Y. Q., Pi, Y., Liu, X., Gao, L., Kang, L., Wang, J., Yang, F., Tang, J., Lu, W., et al. (2017) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.7b00709 · Cited by 67 · see citing works

Biodegradable lipid nanoparticles induce a prolonged RNA interference-mediated protein knockdown and show rapid hepatic clearance in mice and nonhuman primates

Suzuki, Y., Hyodo, K., Suzuki, T., Tanaka, Y., Kikuchi, H., & Ishihara, H. (2017) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2017.01.016 · Cited by 64 · see citing works

Structure, activity and uptake mechanism of siRNA-lipid nanoparticles with an asymmetric ionizable lipid

Suzuki, Y., & Ishihara, H. (2016) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2016.06.124 · Cited by 57 · see citing works

Development of lipid-like materials for RNA delivery based on intracellular environment-responsive membrane destabilization and spontaneous collapse

Tanaka, H., Sakurai, Y., Anindita, J., & Akita, H. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.07.001 · Cited by 56 · see citing works

Lipid Nanoparticle and Liposome Reference Materials: Assessment of Size Homogeneity and Long-Term −70 °C and 4 °C Storage Stability

Jakubek, Z. J., Chen, S., Zaifman, J., Tam, Y. Y. C., & Zou, S. (2023) · Langmuir · doi:10.1021/acs.langmuir.2c02657 · Cited by 52 · see citing works

Lipid-based nanocarriers for delivery of small interfering RNA for therapeutic use

Parashar, D., Rajendran, V., Shukla, R., & Sistla, R. (2019) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2019.105159 · Cited by 47 · see citing works

Lymph node macrophages drive innate immune responses to enhance the anti-tumor efficacy of mRNA vaccines

Kubara, K., Yamazaki, K., Miyazaki, T., Kondo, K., Kurotaki, D., Tamura, T., & Suzuki, Y. (2024) · Molecular Therapy · doi:10.1016/j.ymthe.2024.01.020 · Cited by 50 · see citing works

Long-term stability and immunogenicity of lipid nanoparticle COVID-19 mRNA vaccine is affected by particle size

Shi, R., Liu, X., Wang, Y., Pan, M., Wang, S., Shi, L., & Ni, B. (2024) · Human Vaccines & Immunotherapeutics · doi:10.1080/21645515.2024.2342592 · Cited by 46 · see citing works

Innovative nanotechnologies for enhancing nucleic acids/gene therapy: Controlling intracellular trafficking to targeted biodistribution

Nakamura, T., Yamada, Y., Sato, Y., Khalil, I. A., & Harashima, H. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.119329 · Cited by 44 · see citing works

Particles in Biopharmaceutical Formulations, Part 2: An Update on Analytical Techniques and Applications for Therapeutic Proteins, Viruses, Vaccines and Cells

Roesch, A., Zölls, S., Stadler, D., Helbig, C., Wuchner, K., Kersten, G. F. A., Hawe, A., Jiskoot, W., & Menzen, T. (2021) · Journal of Pharmaceutical Sciences · doi:10.1016/j.xphs.2021.12.011 · Cited by 44 · see citing works

Development of in silico methodology for siRNA lipid nanoparticle formulations

Gao, H., Kan, S., Ye, Z., Feng, Y., Jin, L., Zhang, X., Deng, J., Chan, G., Hu, Y., Wang, Y., et al. (2022) · Chemical Engineering Journal · doi:10.1016/j.cej.2022.136310 · Cited by 42 · see citing works

Charge-reversible lipid derivative: A novel type of pH-responsive lipid for nanoparticle-mediated siRNA delivery

Hirai, Y., Ryoko, S., Song, F., Koide, H., Naofumi, F., Tomita, K., Maeda, N., Oku, N., & Asai, T. (2020) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2020.119479 · Cited by 41 · see citing works

Why mRNA-ionizable LNPs formulations are so short-lived: causes and way-out

De, A., & Ko, Y. T. (2023) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2023.2162876 · Cited by 36 · see citing works

Formulation of biocompatible targeted ECO/siRNA nanoparticles with long-term stability for clinical translation of RNAi

Ayat, N. R., Sun, Z., Sun, D., Yin, M., Hall, R. C., Vaidya, A. M., Liu, X., Schilb, A. L., Scheidt, J. H., & Lu, Z.-R. (2019) · Nucleic Acid Therapeutics · doi:10.1089/nat.2019.0784 · Cited by 33 · see citing works

Simultaneous separation of small interfering RNA and lipids using ion-pair reversed-phase liquid chromatography

Li, L., Foley, J. P., & Helmy, R. (2019) · Journal of Chromatography A · doi:10.1016/j.chroma.2019.04.061 · Cited by 33 · see citing works

Understanding the Manufacturing Process of Lipid Nanoparticles for mRNA Delivery Using Machine Learning

Sato, S., Sano, S., Muto, H., Kubara, K., Kondo, K., Miyazaki, T., Suzuki, Y., Uemoto, Y., & Ukai, K. (2024) · Chemical and Pharmaceutical Bulletin · doi:10.1248/cpb.c24-00089 · Cited by 34 · see citing works

Cytosolic protein delivery using pH-responsive, charge-reversible lipid nanoparticles

Hirai, Y., Hirose, H., Imanishi, M., Asai, T., & Futaki, S. (2021) · Scientific Reports · doi:10.1038/s41598-021-99180-5 · Cited by 28 · see citing works

Toll-like receptor 2 promiscuity is responsible for the immunostimulatory activity of nucleic acid nanocarriers

Pizzuto, M., Gangloff, M., Scherman, D., Gay, N. J., Escriou, V., Ruysschaert, J., & Lonez, C. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.12.029 · Cited by 26 · see citing works

A tale of nucleic acid–ionizable lipid nanoparticles: Design and manufacturing technology and advancement

De, A., & Ko, Y. T. (2022) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2023.2153832 · Cited by 23 · see citing works

Irf5 siRNA-loaded biodegradable lipid nanoparticles ameliorate concanavalin A-induced liver injury

Kawase, W., Kurotaki, D., Suzuki, Y., Ishihara, H., Ban, T., Sato, G. R., Ichikawa, J., Yanai, H., Taniguchi, T., Tsukahara, K., et al. (2021) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2021.08.023 · Cited by 22 · see citing works

Evaluation of the effects of storage conditions on spray-dried siRNA-LNPs before and after subsequent drying

Zimmermann, C. M., Deßloch, L., Jürgens, D. C., Luciani, P., & Merkel, O. M. (2023) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2023.11.007 · Cited by 21 · see citing works

Novel Complement C5 Small-interfering RNA Lipid Nanoparticle Prolongs Graft Survival in a Hypersensitized Rat Kidney Transplant Model

石郷岡秀俊, Katsumata, H., Saiga, K., Tokita, D., Motoi, S., Matsui, C., Suzuki, Y., Tomimatsu, A., Nakatani, T., Kuboi, Y., et al. (2022) · Transplantation · doi:10.1097/tp.0000000000004207 · Cited by 20 · see citing works

Differences and Similarities of the Intravenously Administered Lipid Nanoparticles in Three Clinical Trials: Potential Linkage between Lipid Nanoparticles and Extracellular Vesicles

Suzuki, Y., Katsurada, Y., & Hyodo, K. (2023) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.3c00547 · Cited by 21 · see citing works

Simplifying the Chemical Structure of Cationic Lipids for siRNA-Lipid Nanoparticles

Kuboyama, T., Yagi, K., Naoi, T., Era, T., Yagi, N., Nakasato, Y., Yabuuchi, H., Takahashi, S., Shinohara, F., Iwai, H., et al. (2019) · ACS Medicinal Chemistry Letters · doi:10.1021/acsmedchemlett.8b00652 · Cited by 19 · see citing works

Protective efficacy of a universal influenza mRNA vaccine against the challenge of H1 and H5 influenza A viruses in mice

Li, Y., Wang, X., Zeng, X., Ren, W., Liao, P., & Zhu, B. (2023) · mLife · doi:10.1002/mlf2.12085 · Cited by 16 · see citing works

NANOSPRESSO: toward personalized, locally produced nucleic acid nanomedicines

Sentí, M. E., Ceccaldi, A., Luciani, M., Saber, N., Schürmann, P. J. L., Geerlings, M. W., Hölig, P., de Beer, J., Hannus, M., Campbell, F., et al. (2025) · Frontiers in Science · doi:10.3389/fsci.2025.1458636 · Cited by 17 · see citing works

Ionizable lipid chemistry in lipid nanoparticles determines delivery efficiency to hepatic stellate cells

Muto, H., Kubara, K., Hitaoka, S., Osada, Y., & Suzuki, Y. (2025) · Journal of Controlled Release · doi:10.1016/j.jconrel.2025.114055 · Cited by 17 · see citing works

Anionic Polymer and Quantum Dot Excipients to Facilitate siRNA Release and Self-Reporting of Disassembly in Stimuli-Responsive Nanocarrier Formulations

Greco, C. T., Andrechak, J. C., Epps, T. H. I., & Sullivan, M. O. (2017) · Biomacromolecules · doi:10.1021/acs.biomac.7b00265 · Cited by 16 · see citing works

Lipid nanoparticle mediated small interfering RNA delivery as a potential therapy for Alzheimer's disease

Ahmed, T. (2024) · European Journal of Neuroscience · doi:10.1111/ejn.16336 · Cited by 16 · see citing works

Cellular environment-responsive nanomaterials for use in gene and siRNA delivery: molecular design for biomembrane destabilization and intracellular collapse

Tanaka, H., Sato, Y., Harashima, H., & Akita, H. (2016) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2016.1154531 · Cited by 14 · see citing works

Single pot organic solvent-free thermocycling technology for siRNA-ionizable LNPs: a proof-of-concept approach for alternative to microfluidics

De, A., & Ko, Y. T. (2022) · Drug Delivery · doi:10.1080/10717544.2022.2108523 · Cited by 13 · see citing works

Immune-Modulating Lipid Nanomaterials for the Delivery of Biopharmaceuticals

Kim, S., Choi, B., Kim, Y., & Shim, G. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15061760 · Cited by 13 · see citing works

RNA Modifications in Gastrointestinal Cancer: Current Status and Future Perspectives

Zhang, X., Su, H., Chen, H., Li, Q., Liu, X., Zhang, L., Wu, W. K. K., Chan, M. T. V., & Chen, H. (2022) · Biomedicines · doi:10.3390/biomedicines10081918 · Cited by 13 · see citing works

Identification of potent siRNA targeting complement C5 and its robust activity in pre-clinical models of myasthenia gravis and collagen-induced arthritis

Kuboi, Y., Suzuki, Y., Motoi, S., Matsui, C., Toritsuka, N., Nakatani, T., Tahara, K., Takahashi, Y., Ida, Y., Tomimatsu, A., et al. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.01.005 · Cited by 12 · see citing works

Capillary electrophoresis method to determine siRNA complexation with cationic liposomes

Furst, T., Bettonville, V., Farcaş, E., Frère, A., Lechanteur, A., Évrard, B., Fillet, M., Piel, G., & Servais, A. (2016) · Electrophoresis · doi:10.1002/elps.201600249 · Cited by 11 · see citing works

Optimizing long-term stability of siRNA using thermoassemble ionizable reverse pluronic-Bcl2 micelleplexes

De, A., Kang, J. H., Sauraj, Lee, O. H., & Ko, Y. T. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.130783 · Cited by 9 · see citing works

In Silico Study of Ionizable Lipid Nanoparticles Using the SPICA Force Field

Singh, A. P., Tanaka, H., Miyazaki, Y., Nagano, S., & Shinoda, W. (2025) · Journal of Chemical Theory and Computation · doi:10.1021/acs.jctc.5c00498 · Cited by 8 · see citing works

In vivo production of engineered ACE2 decoy protects lungs from SARS-CoV-2 infection

Suzuki, Y., Miyazaki, T., Ida, Y., Suzuki, T., Itoh, Y., Nakao, S., Kondo, K., Kubara, K., Nishioka, K., Muto, H., et al. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102467 · Cited by 8 · see citing works

Lipid nanoparticles from L. meyenii Walp mitigate sepsis through multimodal protein corona formation

Sung, J. J., Shaw, J. R., Rezende, J. D., Dharmaraj, S., Cottingham, A. L., Weldemariam, M. M., Jones, J. W., Kane, M. A., & Pearson, R. M. (2025) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2025.101491 · Cited by 6 · see citing works

Engineering Lipid Nanoparticles for Precision RNA Delivery: Design Principles, Targeting Strategies, and Clinical Prospects

Han, Y., Wang, Y., Gao, X., Lei, Y., Li, Y., & Tang, Z. (2026) · Cancer Nexus · doi:10.1002/cnx2.70021 · Cited by 6 · see citing works

RP-CAD for Lipid Quantification: Systematic Method Development and Intensified LNP Process Characterization

Beckert, N., Dietrich, A., & Hubbuch, J. J. (2024) · Pharmaceuticals · doi:10.3390/ph17091217 · Cited by 6 · see citing works

Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina

Qtaish, N. A., Villate-Beitia, I., Gallego, I., Martı́nez, G., Soto‐Sánchez, C., Sainz-Ramos, M., López-Méndez, T. B., Paredes, A. J., Chichón, F. J., Zamarreño, N., et al. (2023) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2023.122968 · Cited by 6 · see citing works

Exploring dose and downregulation dynamics in lipid nanoparticles based siRNA therapy: Systematic review and meta-analysis

Kumar, A., Ahmed, B., Kaur, I. P., & Saha, L. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.133984 · Cited by 4 · see citing works

Mitigating Shear Stress in Spray Drying for RNA-Loaded Lipid Nanoparticles through Process and Formulation Optimization

Farinha, S., Mota, R., Lopes, C., Vélez, R., Freire, M. G., Aguiar‐Ricardo, A., Rodrigues, M. Â., Marques, L., Cristóvão, J. S., & Lino, P. R. (2025) · AAPS PharmSciTech · doi:10.1208/s12249-025-03133-4 · Cited by 4 · see citing works

Advances in lipids design for LNP-mediated DNA and RNA delivery

Zhi, D., Halemaimaiti, A., Dai, B., Li, H., Wang, H., Zhang, Z., Yu, J., & Zhang, S. (2026) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2026.103897 · Cited by 2 · see citing works

Development of Dry Powder Inhaler formulation for site specific delivery of nanoconjugates loaded with Curcumin and BCL2 siRNA in Lung Cancer

Lahan, M., Saikia, T., Baishya, R., Bharali, A., Baruah, S., Ghose, S., Biswas, N., Laloo, D., Medhi, S., & Sahu, B. P. (2025) · Pulmonary Pharmacology & Therapeutics · doi:10.1016/j.pupt.2025.102361 · Cited by 1 · see citing works

Ionizable lipids as a promising platform for creating mRNA vaccines

Milagina, S. V., & Puchkov, P. A. (2026) · Fine Chemical Technologies · doi:10.32362/2410-6593-2026-21-1-51-72 · Cited by 1 · see citing works

Advancing the Therapeutic Potential of Swertia Species Through Nanoformulation Strategies

Yadav, A., Sharma, C., & Priyadarshini, E. (2026) · International Journal of Nanoscience · doi:10.1142/s0219581x26300051 · Cited by 1 · see citing works

Creation of innovative nucleic acid medicines from Japan

Suzuki, Y. (2018) · Drug Delivery System · doi:10.2745/dds.33.234

Messenger RNA for Prophylaxis

Jackson, N. A. C. (2022) · RNA technologies · doi:10.1007/978-3-031-08415-7_2

Lipid nanoparticle formulations including stereochemically defined glycomacromolecules for delivery of saRNA

Becker, J., Barbieri, B. D., Shattock, R. J., & Becer, C. R. (2026) · Chemical Science · doi:10.1039/d6sc03833f

Myths and Facts Associated with Vaccine Development: Challenges, Safety, and Efficacy

Vihal, S., Kaur, S. D., Vivek, P., Kumari, N., Kumbham, S., & Salihu, M. B. (2026) · Nanomedicine and nanotoxicology · doi:10.1007/978-3-032-29006-9_15

Storage stability of lipid nanoparticles: a curated benchmark dataset, systematic threshold analysis, and per-study machine learning prediction limits

Adzavon, K. P., Zhao, W., & Sheng, W. (2026) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2026.127405

RNA vaccines in motion: A multidisciplinary exploration of design, delivery, and deployment

Abuhassan, Q., Aldulaimi, A., Waleed, O. S., Ganesan, S., Kavitha, V., Maharana, L., Sharma‎, R., Latipova, M., Madatova, N., Jumanazarov, D., et al. (2026) · Bioimpacts · doi:10.34172/bi.33056

Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage

Clarke, D. T. W., Idris, A., & McMillan, N. A. J. (2019) · PLoS ONE · doi:10.1371/journal.pone.0211954 · Cited by 13 · see citing works

Achieving long-term stability of lipid nanoparticles: examining the effect of pH, temperature, and lyophilization

Ball, R. L., Bajaj, P., & Whitehead, K. A. (2016) · DOAJ (DOAJ: Directory of Open Access Journals)

Stability of lyophilized siRNA nanosome formulations

Kundu, A. K., Chandra, P. K., Hazari, S., Ledet, G. A., Pramar, Y. V., Dash, S., & Mandal, T. K. (2011) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2011.11.040 · Cited by 33 · see citing works

In Vivo Sustained Release of siRNA from Solid Lipid Nanoparticles

Lobovkina, T., Jacobson, G. B., González-González, E., Hickerson, R. P., Leake, D., Kaspar, R. L., Contag, C. H., & Zare, R. N. (2011) · ACS Nano · doi:10.1021/nn203745n · Cited by 139 · see citing works

The Effect of Cryoprotectants and Storage Conditions on the Transfection Efficiency, Stability, and Safety of Lipid‐Based Nanoparticles for mRNA and DNA Delivery

Kafetzis, K. N., Papalamprou, N., McNulty, E., Thong, K. X., Sato, Y., Mironov, A., Purohit, A., Welsby, P. J., Harashima, H., Yu‐Wai‐Man, C., et al. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202203022 · Cited by 96 · see citing works