Age-Dependent Decrease of Mitochondrial Complex II Activity in Human Skin Fibroblasts
Open accessAbstract
The mitochondrial theory of aging remains one of the most widely accepted aging theories and implicates mitochondrial electron transport chain dysfunction with subsequent increasing free radical generation. Recently, complex II of the electron transport chain appears to be more important than previously thought in this process, suggested predominantly by nonhuman studies. We investigated the relationship between complex II and aging using human skin as a model tissue. The rate of complex II activity per unit of mitochondria was determined in fibroblasts and keratinocytes cultured from skin covering a wide age range. Complex II activity significantly decreased with age in fibroblasts (P = 0.015) but not in keratinocytes. This was associated with a significant decline in transcript expression (P = 0.008 and P = 0.001) and protein levels (P = 0.0006 and P = 0.005) of the succinate dehydrogenase complex subunit A and subunit B catalytic subunits of complex II, respectively. In addition, there was a significant decrease in complex II activity with age (P = 0.029) that was specific to senescent skin cells. There was no decrease in complex IV activity with increasing age, suggesting possible locality to complex II.
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Mitochondrial complex II activity decreases with age in human skin fibroblasts, an effect only observed in senescent cell populations.
“Bowman et al. demonstrated that complex II activity decreases with age in human skin fibroblasts, an effect only observed in senescent cell populations [23].”
From Oxidative Stress and Ageing: The Influence of Environmental Pollution, Sunlight and Diet on Skin (2017) · cites this paper as [23]
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- Authors
- Bowman, A., & Birch-Machin, M. A.
- Affiliations
- Newcastle University (GB)
- ORCID
- Bowman, Amy
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 136
- Issue
- 5
- Pages
- 912-919
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2239153527
- Cited by
- 60
- References
- 46
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 6 · 2023: 6 · 2022: 8 · 2021: 5 · 2020: 3 · 2019: 7 · 2018: 5 · 2017: 7 · 2016: 4
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Newcastle University ePrints (Newcastle Univesity) (cc-by-nc-nd)
- Funders
- National Institute for Health and Care Research
- Newcastle University
- Fakultet Medicinskih Nauka, Univerziteta U Kragujevcu
- NIHR Newcastle Biomedical Research Centre
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Keywords
- succinate dehydrogenase, aging, dermal fibroblasts, keratinocytes, mitochondrial electron transport chain, mitochondrial theory of aging, senescence, cytochrome c oxidase, gene expression, free radicals
- MeSH terms
- Cells, Cultured, Female, Fibroblasts, Gene Expression Regulation, Humans, Male, Metabolism, Inborn Errors, Mitochondria, Sensitivity and Specificity, Spectrophotometry, Biomarkers, Skin Aging, Keratinocytes, Risk Assessment, Mitochondrial Diseases, Electron Transport Complex II
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