Development of HPLC analytical protocols for quantification of artemisinin in biomass and extracts
Abstract
Quantification of artemisinin purity and amount in plant material and extracts to date has been characterized by a considerable inconsistency in values. This is likely to be due to the adoption of varied analytical procedures and use of inappropriate to the specific applications analytical techniques. In this paper we are attempting to further develop artemisinin analysis to the point where a universally acceptable reference method is available to the research and end-users communities. Thus, we have developed and validated an HPLC-RI method and optimized an HPLC-ELSD method. We used the gradient HPLC-UV method recommended by the current artemisinin monograph as a comparison for the method improvements presented herein, and show the limitations for its application scope. The data reported should help to allow more reliable laboratory analysis of artemisinin in both pure samples and in Artemisia annua extracts.
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Many investigators use the HPLC Q260 method of analysis, but it can result in false positives regarding artemisinin mainly because it cannot distinguish between artemisinin and deoxyartemisinin.
“Many investigators use the HPLC Q260 method of analysis, but it can result in false positives regarding artemisinin mainly because it cannot distinguish between artemisinin and deoxyartemisinin (Lapkin et al. 2009; Christen and Veuthey 2001; Smith et al. 2010).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Lapkin et al. 2009
There is considerable inconsistency in the amount of quantified artemisinin present in extracts depending on the use of different analytical methods.
“There is considerable inconsistency in the amount of quantified artemisinin present in the extracts depending on the use of different analytical methods (Lapkin et al. 2009) and thus researchers need to use more definitive assays that validate artemisinin content.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Lapkin et al. 2009
Cites
Comparative Assessment of Technologies for Extraction of Artemisinin
Lapkin, A. A., Plucinski, P. K., & Cutler, M. (2006) · Journal of Natural Products · doi:10.1021/np060375j · Cited by 181 · see citing works
Self-citationBilia, A. R., de Magalhães, P. M., Bergonzi, M. C., & Vincieri, F. F. (2006) · Phytomedicine · doi:10.1016/j.phymed.2006.01.008 · Cited by 115 · see citing works
New Trends in Extraction, Identification and Quantification of Artemisinin and its Derivatives
Christen, P., & Veuthey, J. (2001) · Current Medicinal Chemistry · doi:10.2174/0929867013371563 · Cited by 114 · see citing works
Ma, C., Wang, H., Lu, X., Xu, G., & Liu, B. (2007) · Journal of Chromatography A · doi:10.1016/j.chroma.2007.09.023 · Cited by 103 · see citing works
Reductive Electrochemical HPLC Assay for Artemisinin (Qinghaosu)
Acton, N., Klayman, D. L., & Rollman, I. J. (1985) · Planta Medica · doi:10.1055/s-2007-969545 · Cited by 87 · see citing works
ElSohly, H. N., Croom, E. M., & ElSohly, M. A. (1987) · Pharmaceutical Research · doi:10.1023/a:1016472531527 · Cited by 75 · see citing works
Avery, B. A., Venkatesh, K. K., & Avery, M. A. (1999) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(99)00185-1 · Cited by 70 · see citing works
Liu, C., Zhou, H.-Y., & Zhao, Y. (2006) · Analytica Chimica Acta · doi:10.1016/j.aca.2006.08.038 · Cited by 69 · see citing works
Analysis of Artemisinin in Artemisia annua L. by LC-MS with Selected Ion Monitoring
Wang, M., Park, C., Wu, Q., & Simon, J. E. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf051061p · Cited by 59 · see citing works
Petersen, F., & Amstutz, R. (2008) · Birkhäuser Basel eBooks · doi:10.1007/978-3-7643-8595-8 · Cited by 55 · see citing works
Zhao, S. (1987) · The Analyst · doi:10.1039/an9871200661 · Cited by 52 · see citing works
Quantification of artemisinin in Artemisia annua extracts by 1H‐NMR
Castilho, P. C., Gouveia, S. C., & Rodrigues, A. I. (2008) · Phytochemical Analysis · doi:10.1002/pca.1053 · Cited by 48 · see citing works
Determination of Artemisinin in Artemisia annua L. by Reversed Phase HPLC
Qian, G., Yang, Y., & Ren, Q. (2005) · Journal of Liquid Chromatography & Related Technologies · doi:10.1081/jlc-200048890 · Cited by 46 · see citing works
Chemical Instability Determines the Biological Action of the Artemisinins
Jansen, F. H., & Soomro, S. A. (2007) · Current Medicinal Chemistry · doi:10.2174/092986707782793844 · Cited by 38 · see citing works
Simultaneous Determination of Artemisinin and Its Bioprecursors in Artemisia annua
Vandenberghe, D., Vergauwe, A., Montagu, M. V., & den Eeckhout, E. G. V. (1995) · Journal of Natural Products · doi:10.1021/np50119a027 · Cited by 36 · see citing works
Solubility of Artemisinin in Ethanol + Water from (278.2 to 343.2) K
Wang, L., Song, Y., Chen, Y., et al. (2007) · Journal of Chemical & Engineering Data · doi:10.1021/je0603426 · Cited by 34 · see citing works
HPLC−Electrospray Ionization Mass Spectrometric Analysis of Antimalarial Drug Artemisinin
Sahai, P., Vishwakarma, R. A., Bharel, S., Gulati, A., Abdin, M. Z., Srivastava, P. S., & Jain, S. K. (1998) · Analytical Chemistry · doi:10.1021/ac970944f · Cited by 33 · see citing works
Quantitative HPTLC Analysis of Artemisinin in Dried<i>Artemisia annua</i>L.: A Practical Approach
Widmer, V., Handloser, D., & Reich, E. (2007) · Journal of Liquid Chromatography & Related Technologies · doi:10.1080/10826070701451555 · Cited by 20 · see citing works
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Wani, K. I., Choudhary, S., Zehra, A., Naeem, M., Weathers, P., & Aftab, T. (2021) · Planta · doi:10.1007/s00425-021-03676-3 · Cited by 74 · see citing works
Domokos, E., Jakab-Farkas, L., Darkó, B., Bíró-Janka, B., Mara, G., Albert, C., & Balog, A. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00478 · Cited by 52 · see citing works
Expression of key genes affecting artemisinin content in five Artemisia species
Salehi, M., Karimzadeh, G., Naghavi, M. R., Naghdi Badi, H., & Rashidi Monfared, S. (2018) · Scientific Reports · doi:10.1038/s41598-018-31079-0 · Cited by 44 · see citing works
Khan, S., Ali, A., Ahmad, S., & Abdin, M. Z. (2015) · Biomedical Chromatography · doi:10.1002/bmc.3465 · Cited by 21 · see citing works
Suberu, J., Gromski, P. S., Nordon, A., & Lapkin, A. (2016) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2015.10.003 · Cited by 19 · see citing works
Self-citation
- Authors
- Lapkin, A. A., Walker, A., Sullivan, N., Khambay, B., Mlambo, B., & Chemat, S.
- Affiliations
- University of Bath (GB)
- Rothamsted Research (GB)
- Corresponding author
- Lapkin, Alexei A.
- Venue
- Journal of Pharmaceutical and Biomedical Analysis
- Publisher
- Elsevier BV
- Published
- 2009-05
- Volume
- 49
- Issue
- 4
- Pages
- 908-915
- ISSN
- 0731-7085 (ISSN-L)
- OpenAlex
- W2088418994
- Cited by
- 78
- References
- 18
- Citations by year
- 2025: 4 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 6 · 2020: 4 · 2019: 7 · 2018: 10 · 2017: 6 · 2016: 2 · 2015: 6 · 2014: 3 · 2013: 4 · 2012: 7
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical and Biomedical Analysis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- GlaxoSmithKline
- Fundación Mundo Sano
- Engineering and Physical Sciences Research Council (EP/F016182/1)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Keywords
- artemisinin, HPLC method development, HPLC-ELSD, HPLC-UV, method validation, Artemisia annua, reference method, plant extracts, analytical method comparison
- MeSH terms
- Antimalarials, Chromatography, High Pressure Liquid, Plant Extracts, Spectrophotometry, Ultraviolet, Mass Spectrometry, Reproducibility of Results, Biomass, Artemisinins
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