Multivariate data analysis and metabolic profiling of artemisinin and related compounds in high yielding varieties of Artemisia annua field-grown in Madagascar
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An improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) protocol for rapid analysis of co-metabolites of A. annua in raw extracts was developed and extensively characterized. The new method was used to analyse metabolic profiles of 13 varieties of A. annua from an in-field growth programme in Madagascar. Several multivariate data analysis techniques consistently show the association of artemisinin with dihydroartemisinic acid. These data support the hypothesis of dihydroartemisinic acid being the late stage precursor to artemisinin in its biosynthetic pathway.
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Two hybrid varieties Hyb8001r (Zenith) and Hyb1209r (Shennong) with 54.5 and 36.3 kg/ha artemisinin were commercially released, with 8001r outperforming 12 other cultivars.
“After rigorous procedures of selection two hybrid varieties namely Hyb8001r (Zenith) and Hyb1209r (Shennong) with 54.5 and 36.3 kg/ha artemisinin, respectively, were commercially released with 8001r outperforming 12 other cultivars (Suberu et al. 2016).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Suberu et al. 2016
Section 2.3 (Plant varieties) identifies CNAP8001R and CNAP1209R among 13 varieties evaluated to provide crosses for new hybrids. Section 3.3 reports the highest measured artemisinin concentration for CNAP8001, supporting its lead over the other sampled varieties on that measure. The paper does not report the claimed kg/ha yields, the names Zenith and Shennong, or commercial release; its concentration measurements do not establish field yield. (LLM, conf=0.88)
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- Authors
- Suberu, J., Gromski, P. S., Nordon, A., & Lapkin, A.
- Affiliations
- University of Cambridge (GB)
- University of Strathclyde (GB)
- Corresponding author
- Lapkin, Alexei
- Venue
- Journal of Pharmaceutical and Biomedical Analysis
- Publisher
- Elsevier BV
- Published
- 2016-01
- Volume
- 117
- Pages
- 522-531
- ISSN
- 0731-7085 (ISSN-L)
- OpenAlex
- W2103868696
- Cited by
- 19
- References
- 46
- Citations by year
- 2024: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 5
- FWCI
- 0.99
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Journal of Pharmaceutical and Biomedical Analysis (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Strathprints: The University of Strathclyde institutional repository (University of Strathclyde) (submittedVersion)
- Apollo (University of Cambridge) (submittedVersion, cc-by)
- Funders
- Engineering and Physical Sciences Research Council (EP/L003309/1)
- Medicines for Malaria Ventures
- University of Cambridge
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Keywords
- artemisinin, Artemisia annua, dihydroartemisinic acid, artemisinin biosynthesis, metabolite profiling, high-yielding varieties, field-grown plants, LC-MS/MS method development, multivariate analysis
- MeSH terms
- Madagascar, Plant Extracts, Statistics as Topic, Multivariate Analysis, Plant Leaves, Artemisia annua, Metabolomics
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