Determination of mangiferin in rat plasma by liquid-liquid extraction with UPLC-MS/MS
Abstract
An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method employing electrospray ionization (ESI) has been developed for the determination of mangiferin in rat plasma using diphenhydramine as the internal standard (IS). Liquid-liquid extraction (LLE) was used for sample preparation and the analysis was achieved with gradient elution on C(18) reversed phase column. The method was validated over the concentration range 0.02-5.0 microg/mL for oral administration and 0.4-100 microg/mL for intravenous administration. The intra-day and inter-day precision of mangiferin expressed as RSD<15% and the accuracy (RE) did not exceed 15%. This validated method is a novel technique for sample preparation and quantitation, which was successfully applied to estimate the bioavailability of mangiferin.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The oral bioavailability of mangiferin is only 1.2%.
“Han et al (7) demonstrated that the oral bioavailability of mangiferin was only 1.2%.”
From Mangiferin: An effective therapeutic agent against several disorders (Review) (2018) · cites this paper as [7]
Cites
Immunomodulatory activity of alcoholic extract of Mangifera indica L. in mice
Makare, N., Bodhankar, S., & Rangari, V. (2001) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(01)00326-9 · Cited by 258 · see citing works
Schieber, A., Berardini, N., & Carle, R. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf030218f · Cited by 235 · see citing works
Yoshimi, N., Matsunaga, K., Katayama, M., Yamada, Y., Kuno, T., Qiao, Z., Hara, A., Yamahara, J., & Mori, H. (2001) · Cancer Letters · doi:10.1016/s0304-3835(00)00678-9 · Cited by 214 · see citing works
Antidiabetic activity of a xanthone compound, mangiferin
Miura, T., Ichiki, H., Hashimoto, I., Iwamoto, N., Kao, M., Kubo, M., Ishihara, E., Komatsu, Y., Okada, M., Ishida, T., et al. (2001) · Phytomedicine · doi:10.1078/0944-7113-00009 · Cited by 206 · see citing works
New Antidiabetic Compounds, Mangiferin and Its Glucoside.
Ichiki, H., Miura, T., Kubo, M., Ishihara, E., KOMATSU, Y., Tanigawa, K., & Okada, M. (1998) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.21.1389 · Cited by 158 · see citing works
MIURA, T., ICHIKI, H., IWAMOTO, N., KATO, M., KUBO, M., SASAKI, H., OKADA, M., ISHIDA, T., SEINO, Y., & TANIGAWA, K. (2001) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.24.1009 · Cited by 137 · see citing works
Bairy, I., Reeja, S., Siddharth, Rao, P. S., Bhat, M., & Shivananda, P. G. (2002) · PubMed · Cited by 42 · see citing works
Suryawanshi, S., Asthana, R. K., & GUPTA, R. C. (2007) · Journal of Chromatography B · doi:10.1016/j.jchromb.2007.08.034 · Cited by 39 · see citing works
Wang, H., Ye, G., Tang, Y., Zhu, H., Ma, R., Sun, Z.-L., & Huang, C. (2006) · Biomedical Chromatography · doi:10.1002/bmc.695 · Cited by 35 · see citing works
LC Determination and Pharmacokinetics Study of Mangiferin in Rat Plasma and Tissues
Li, Y., Sui, Y.-J., Dai, Y.-H., et al. (2008) · Chromatographia · doi:10.1365/s10337-008-0612-8 · Cited by 19 · see citing works
Li, Y. J., & Bi, K. (2003) · Chromatographia · doi:10.1007/bf02491763 · Cited by 18 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Mangiferin and Cancer: Mechanisms of Action
Gold-Smith, F., Fernandez, A., & Bishop, K. (2016) · Nutrients · doi:10.3390/nu8070396 · Cited by 158 · see citing works
Mangiferin: An effective therapeutic agent against several disorders (Review)
Du, S., Liu, H., Lei, T., Xie, X., Wang, H., He, X., Tong, R., & Wang, Y. (2018) · Molecular Medicine Reports · doi:10.3892/mmr.2018.9529 · Cited by 139 · see citing works
“In addition, Han et al (7) demonstrated that the oral bioavailability of mangiferin was only 1.2%.”
InfluentialImproving permeability and oral absorption of mangiferin by phospholipid complexation
Ma, H., Chen, H., Sun, L., Tong, L., & Zhang, T. (2014) · Fitoterapia · doi:10.1016/j.fitote.2013.10.016 · Cited by 108 · see citing works
Li, J., Liu, M., Yu, H., Wang, W., Han, L., Chen, Q., Ruan, J., Wen, S., Zhang, Y., & Wang, T. (2018) · Frontiers in Pharmacology · doi:10.3389/fphar.2018.00201 · Cited by 95 · see citing works
Self-citationMorozkina, S. N., Nhung Vu, T. H., Generalova, Y. E., Snetkov, P. P., & Uspenskaya, M. V. (2021) · Biomolecules · doi:10.3390/biom11010079 · Cited by 82 · see citing works
Liu, H., Wu, B., Pan, G., He, L., Li, Z., Fan, M., Jian, L., Chen, M., Wang, K., & Huang, C. (2012) · Drug Metabolism and Disposition · doi:10.1124/dmd.112.045849 · Cited by 77 · see citing works
The management of diabetes mellitus by mangiferin: advances and prospects
Wang, M., Liang, Y., Chen, K., Wang, M., Long, X., Liu, H., Sun, Y., & He, B. (2022) · Nanoscale · doi:10.1039/d1nr06690k · Cited by 56 · see citing works
Natural antioxidants’ effects on endoplasmic reticulum stress-related diseases
Reyes-Fermín, L. M., Aparicio-Trejo, O. E., Avila-Rojas, S. H., Gómez-Sierra, T., Martínez-Klimova, E., & Pedraza-Chaverri, J. (2020) · Food and Chemical Toxicology · doi:10.1016/j.fct.2020.111229 · Cited by 50 · see citing works
Bunt, D., Schwalbe, M., Hayeeawaema, F., & El Aidy, S. (2025) · Gut Microbes · doi:10.1080/19490976.2025.2508422 · Cited by 10 · see citing works
- Authors
- Han, D., Chen, C., Zhang, C., Zhang, Y., & Tang, X.
- Affiliations
- Shenyang Pharmaceutical University (CN)
- Corresponding author
- Tang, Xing
- Venue
- Journal of Pharmaceutical and Biomedical Analysis
- Publisher
- Elsevier BV
- Published
- 2010-01
- Volume
- 51
- Issue
- 1
- Pages
- 260-263
- ISSN
- 0731-7085 (ISSN-L)
- OpenAlex
- W2040331070
- Semantic Scholar
- 653a829c9ba9563abbdd49c60809873f279d17a7
- Cited by
- 118
- References
- 11
- Citations by year
- 2026: 5 · 2025: 13 · 2024: 2 · 2023: 14 · 2022: 8 · 2021: 7 · 2020: 9 · 2019: 12 · 2018: 6 · 2017: 1 · 2016: 11 · 2015: 5 · 2014: 3 · 2013: 5 · 2012: 4
- FWCI
- 12.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical and Biomedical Analysis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Keywords
- mangiferin, rat plasma, bioavailability, liquid-liquid extraction, UPLC-MS/MS, sample preparation, method validation, pharmacokinetics
- MeSH terms
- Administration, Oral, Animals, Biological Availability, Chromatography, High Pressure Liquid, Diphenhydramine, Dose-Response Relationship, Drug, Injections, Intravenous, Male, Rats, Wistar, Spectrometry, Mass, Electrospray Ionization, Xanthones, Rats, Tandem Mass Spectrometry
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
