Malvidin and cyanidin derivatives from açai fruit (Euterpe oleracea Mart.) counteract UV-A-induced oxidative stress in immortalized fibroblasts
Abstract
UV-A radiations are known to induce cellular oxidative stress, leading to premature skin aging. Consumption of açai fruit (Euterpe oleracea Martius) is known to have many health benefits due to its high level of antioxidants. Herein, we analyzed the ability of phenolic compounds extracted from this fruit to attenuate UV-A-induced oxidative stress in immortalized fibroblast. A methanol/water açai extract was fractionated by HPLC and each fraction tested for anti-oxidant stress activity. Immortalized fibroblasts were pre-incubated with açai fractions and then exposed to UV-A radiations. Açai extract was found to be able to strongly protect cells from oxidative stress. In particular, reactive oxygen species (ROS) production, GSH depletion, lipid peroxidation and no increase in the phosphorylation levels of proteins involved in the oxidative stress pathway was observed in cells pre-incubated with the extract and then irradiated by UV-A. Mass spectrometry analyses of HPLC fractionated extract led us to the identification of malvidin and cyanidin derivatives as the most active molecules able to counteract the negative effects induced by UV-A irradiation. Our results indicate, for the first time, that açai fruit is a valuable natural source for malvidin and cyanidin to be used as anti-stress molecules and represent good candidates for dietary intervention in the prevention of age related skin damage.
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Malvidin and cyanidin from açai were able to counteract UVA-induced oxidative stress in immortalized fibroblasts.
“We recently demonstrated that two phenolic compounds, malvidin and cyanidin, extracted from fruits of the açai tree (Euterpe oleracea Mart.), a South American palm, were able to counteract UVA-induced oxidative stress in immortalized fibroblasts [74].”
From Antioxidants from Plants Protect against Skin Photoaging (2018) · cites this paper as [74]
Preincubation of UVA-irradiated BALB/3T3 cells with açai phenolic compounds interfered with ROS production and kept GSH levels and lipid peroxidation comparable to normal cellular levels.
“Indeed, the preincubation of UVA-irradiated BALB/3T3 cells with açai phenolic compounds interfered with ROS production and kept GSH levels and lipid peroxidation comparable to normal cellular levels [74].”
From Antioxidants from Plants Protect against Skin Photoaging (2018) · cites this paper as [74]
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- Authors
- Petruk, G., Illiano, A., Del Giudice, R., Raiola, A., Amoresano, A., Rigano, M. M., Piccoli, R., & Monti, D. M.
- Affiliations
- University of Naples Federico II (IT)
- Istituto Nazionale Biostrutture e Biosistemi (IT)
- Corresponding author
- Monti, Daria Maria
- ORCID
- Venue
- Journal of Photochemistry and Photobiology B: Biology
- Publisher
- Elsevier BV
- Published
- 2017-07
- Volume
- 172
- Pages
- 42-51
- ISSN
- 1011-1344 (ISSN-L)
- OpenAlex
- W2613034412
- Cited by
- 50
- References
- 64
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 7 · 2022: 8 · 2021: 7 · 2020: 7 · 2019: 4 · 2018: 6 · 2017: 1
- FWCI
- 5.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Photochemistry and Photobiology B Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- açaí, Euterpe oleracea, malvidin, cyanidin derivatives, anthocyanins, UV-A radiation, oxidative stress, fibroblasts, reactive oxygen species, lipid peroxidation, glutathione depletion, skin aging, dietary antioxidants, phenolic compounds
- MeSH terms
- Animals, Anthocyanins, Antioxidants, Cell Survival, Chromatography, High Pressure Liquid, Fruit, Phosphorylation, Plant Extracts, Mass Spectrometry, Ultraviolet Rays, 3T3 Cells, Reactive Oxygen Species, Oxidative Stress, Mice, Chromatography, Reverse-Phase, Euterpe
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