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Cited by 24Nicotinic Acetylcholine Receptors StudyNeuroscience and Neuropharmacology ResearchNeural dynamics and brain function

Nicotinic activity stabilizes convergence of nicotinic and GABAergic synapses on filopodia of hippocampal interneurons

Zago, Wagner M., Massey, Kerri A., Berg, Darwin K.·2006·Molecular and Cellular Neuroscience
DOI 10.1016/j.mcn.2005.11.009
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Abstract

Nicotinic acetylcholine receptors containing alpha7 subunits occupy pre- and postsynaptic sites in the adult hippocampus. We find that embryonic hippocampal slices in culture display the receptors most prominently on interneurons where they form clusters localized in part on filopodia. The receptors often co-distribute specifically with GABAA receptors. In septal-hippocampal co-cultures, the filopodia become co-innervated by cholinergic and GABAergic terminals abutting the receptor clusters. Nicotinic transmission appears to stabilize the cholinergic contacts: pharmacological blockade of the alpha7-containing nicotinic receptors increases the rate of filopodia movement and decreases the incidence of the clusters being adjacent to cholinergic terminals. Immunostaining fresh hippocampal slices from neonatal rat pups confirms that cholinergic and GABAergic terminals contact alpha7-containing nicotinic receptor clusters in vivo, and the clusters appear to include filopodial sites. The results indicate a convergence of nicotinic and GABAergic input at specific sites on developing hippocampal interneurons and suggest that synaptic activity helps stabilize the nicotinic contribution.

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  • Nicotinic acetylcholine receptors contribute to the maturation of GABAergic and glutamatergic synapses.

    “Recent studies have also shown that nAChRs contribute to the maturation of GABAergic (Kawai et al., 2002; Zago et al., 2006) and glutamatergic (Lozada et al., 2012a, b) synapses, highlighting an important role for ACh signaling in synaptic development, as well as neuronal pathfinding and target selection (reviewed in (Role and Berg, 1996).”

    From Acetylcholine as a Neuromodulator: Cholinergic Signaling Shapes Nervous System Function and Behavior (2012) · cites this paper as Zago et al., 2006

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Authors
Zago, W. M., Massey, K. A., & Berg, D. K.
Affiliations
University of California San Diego (US)
Corresponding author
Berg, Darwin K.
ORCID
Zago, Wagner M.
Venue
Molecular and Cellular Neuroscience
Publisher
Elsevier BV
Published
2006-03
Volume
31
Issue
3
Pages
549-559
ISSN
1044-7431 (ISSN-L)
DOI
10.1016/j.mcn.2005.11.009
OpenAlex
W2171712409
Cited by
24
References
65
Citations by year
2025: 1 · 2018: 2 · 2017: 1 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 2
FWCI
1.36
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Molecular and Cellular Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke (NS12601, NS35469, R01NS012601, R01NS035469)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
nicotinic acetylcholine receptors, alpha7 nicotinic acetylcholine receptor, GABAA receptor, synaptic convergence, filopodia, hippocampal interneurons, cholinergic nerve terminals, GABAergic terminals, nicotinic transmission
MeSH terms
Acetylcholine, Animals, Animals, Newborn, Cholinergic Fibers, gamma-Aminobutyric Acid, Hippocampus, Interneurons, Synaptic Transmission, Organ Culture Techniques, Pseudopodia, Receptors, GABA-A, Receptors, Nicotinic, Synapses, Rats, Sprague-Dawley, Presynaptic Terminals, Nicotinic Antagonists, GABA Antagonists, Coculture Techniques, Receptor Cross-Talk, Dendritic Spines, Rats, alpha7 Nicotinic Acetylcholine Receptor

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