The Dynamics of Dendritic Structure in Developing Hippocampal Slices
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Time-lapse fluorescence confocal microscopy was used to directly visualize the formation and dynamics of postsynaptic target structures (i.e., dendritic branches and spines) on pyramidal neurons within developing tissue slices. Within a 2 week period of time, pyramidal neurons in cultured slices derived from early postnatal rat (postnatal days 2-7) developed complex dendritic arbors bearing numerous postsynaptic spines. At early stages (1-2 d in vitro), many fine filopodial protrusions on dendrite shafts rapidly extended (maximum rate approximately 2.5 microM/minute) and retracted (median filopodial lifetime, 10 min), but some filopodia transformed into growth cones and nascent dendrite branches. As dendritic arbors matured, the population of fleeting lateral filopodia was replaced by spine-like structures having a low rate of turnover. This developmental progression involved a transitional stage in which dendrites were dominated by persistent (up to 22 hr) but dynamic spiny protrusions (i.e., protospines) that showed substantial changes in length and shape on a timescale of minutes. These observations reveal a highly dynamic state of postsynaptic target structures that may actively contribute to the formation and plasticity of synaptic connections during CNS development.
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Individual spines undergo shape changes on the order of seconds to minutes.
“individual spines undergo shape changes on the order of seconds to minutes (Hosokawa et al., 1992;Dailey and Smith, 1996)”
From Regulation of F-Actin Stability in Dendritic Spines by Glutamate Receptors and Calcineurin (1998) · cites this paper as Dailey and Smith, 1996
The persistence of synaptic actin punctae in cytochalasin D correlates with the observed persistence of individual dendritic spines over similar time periods.
“This result correlates with the observed persistence of individual dendritic spines over similar time periods (Hosokawa et al., 1992;Dailey and Smith, 1996).”
From Regulation of F-Actin Stability in Dendritic Spines by Glutamate Receptors and Calcineurin (1998) · cites this paper as Dailey and Smith, 1996
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- Authors
- Dailey, M. E., & Smith, S. J.
- Affiliations
- Stanford Medicine (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1996-05-01
- Volume
- 16
- Issue
- 9
- Pages
- 2983-2994
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6579052
- OpenAlex
- W2130183688
- Cited by
- 611
- References
- 0
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 8 · 2023: 12 · 2022: 11 · 2021: 14 · 2020: 13 · 2019: 14 · 2018: 12 · 2017: 18 · 2016: 21 · 2015: 18 · 2014: 20 · 2013: 27 · 2012: 25
- FWCI
- 5.69
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (2r01ns028587-08, 5p50mh048108-07, 1f32ns009027-01, MH48108)
- National Institute of Mental Health (MH 48108)
- National Institute of Neurological Disorders and Stroke (NS28587, NS09027)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Keywords
- dendritic development, dendritic spines, filopodia, growth cones, pyramidal neurons, hippocampal slices, dendritic branching, synaptic plasticity, postnatal rat brain
- MeSH terms
- Animals, Animals, Newborn, Dendrites, Hippocampus, Microscopy, Fluorescence, Microscopy, Confocal, Culture Techniques, Rats
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