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Cited by 271Nicotinic Acetylcholine Receptors StudyNeuroscience and Neuropharmacology ResearchIon channel regulation and function

Functional nicotinic ACh receptors on interneurones in the rat hippocampus

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Jones, Susan, Yakel, Jerrel L.·1997·The Journal of Physiology
DOI 10.1111/j.1469-7793.1997.603bd.xPMC PMC1159964
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Abstract

Neuronal nicotinic ACh receptors (nAChRs) were studied in the rat hippocampal slice preparation using whole‐cell patch‐clamp recording techniques. Responses to ACh (100 μ m) were detected on inhibitory interneurones in the CA1 field of the hippocampus proper and in the dentate gyrus, but not on principal excitatory neurones in either region. The different neuronal types were identified based on their morphology and location. ACh excited interneurones in the hippocampus and dentate gyrus in current‐clamp recordings. In voltage‐clamp recordings, ACh‐activated inward currents were recorded from interneurones in the presence of blockers of synaptic transmission and the muscarinic ACh receptor antagonist atropine. The zero current potential for this response to ACh was near 0 mV. The effect of ACh was mimicked by the nAChR‐selective agonists nicotine (100 μ m) and 1,1‐dimethyl‐4‐phenyl‐piperazinium iodide (DMPP, 100 μ m). The response to ACh was reversibly antagonized by the neuronal nAChR antagonist mecamylamine (10 μ m). The nAChR α7 subunit‐selective antagonists α‐bungarotoxin (100 n m) and methyllycaconitine (10 n m) also inhibited the response to ACh. These observations demonstrate the presence of functional nAChRs on inhibitory interneurones in the rat hippocampus. Thus, a novel mechanism by which ACh can regulate neuronal activity in the hippocampus is revealed.

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  • Increased inhibition through nAChR activation on interneurons occurs in many brain regions.

    “Increased inhibition through activation of nAChRs expressed by interneurons has been found in many different brain regions (Jones and Yakel 1997; Xiang et al. 1998; McQuiston and Madison 1999; Alkondon et al. 2000; Ji and Dani 2000; Mansvelder et al. 2002; Gulledge et al. 2007).”

    From Short- and Long-Term Consequences of Nicotine Exposure during Adolescence for Prefrontal Cortex Neuronal Network Function (2012) · cites this paper as Jones and Yakel 1997

    The Abstract reports that nAChR activation excited inhibitory interneurons in the rat hippocampus and dentate gyrus. It does not report a measured increase in inhibition, and this paper alone addresses only those hippocampal regions, not many brain regions. (LLM, conf=0.85)

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Sorted by citations

Authors
Jones, S., & Yakel, J. L.
Affiliations
National Institutes of Health (US)
Corresponding author
Yakel, Jerrel L.
ORCID
Venue
The Journal of Physiology
Publisher
Wiley
Published
1997-11
Volume
504
Issue
3
Pages
603-610
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1111/j.1469-7793.1997.603bd.x
PMC
PMC1159964
OpenAlex
W2050455764
Cited by
271
References
1
Citations by year
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FWCI
4.99
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 2525 d)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
nicotinic acetylcholine receptors, hippocampus, inhibitory interneurons, acetylcholine, dentate gyrus, hippocampal CA1, mecamylamine, alpha-bungarotoxin, methyllycaconitine, nicotine, DMPP
MeSH terms
Animals, Electric Stimulation, Electrophysiology, Hippocampus, Interneurons, Membrane Potentials, Receptors, Nicotinic, Rats, Wistar, Patch-Clamp Techniques, Nicotinic Agonists, Nicotinic Antagonists, Dentate Gyrus, Rats, In Vitro Techniques

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