Cooperative and independent roles of the Drp1 adaptors Mff, MiD49 and MiD51 in mitochondrial fission
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Cytosolic dynamin-related protein 1 (Drp1, also known as DNM1L) is required for both mitochondrial and peroxisomal fission. Drp1-dependent division of these organelles is facilitated by a number of adaptor proteins at mitochondrial and peroxisomal surfaces. To investigate the interplay of these adaptor proteins, we used gene-editing technology to create a suite of cell lines lacking the adaptors MiD49 (also known as MIEF2), MiD51 (also known as MIEF1), Mff and Fis1. Increased mitochondrial connectivity was observed following loss of individual adaptors, and this was further enhanced following the combined loss of MiD51 and Mff. Moreover, loss of adaptors also conferred increased resistance of cells to intrinsic apoptotic stimuli, with MiD49 and MiD51 showing the more prominent role. Using a proximity-based biotin labeling approach, we found close associations between MiD51, Mff and Drp1, but not Fis1. Furthermore, we found that MiD51 can suppress Mff-dependent enhancement of Drp1 GTPase activity. Our data indicates that Mff and MiD51 regulate Drp1 in specific ways to promote mitochondrial fission.
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In mammals, there are no orthologues for MDV1 and Cav4, and recent evidence suggests that Fis1 is not required for fission.
“In mammals, there are no orthologues for MDV1 and Cav 4, and recent evidence suggests that Fis1 is not required for fission [89, 90].”
From The connection between the dynamic remodeling of the mitochondrial network and the regulation of muscle mass (2021) · cites this paper as [89]
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Self-citation
- Authors
- Osellame, L. D., Singh, A. P., Stroud, D. A., Palmer, C. S., Stojanovski, D., Ramachandran, R., & Ryan, M. T.
- Affiliations
- Monash University (AU)
- La Trobe University (AU)
- Burnet Institute (AU)
- The University of Melbourne (AU)
- Case Western Reserve University (US)
- ORCID
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2016-06-01
- Volume
- 129
- Issue
- 11
- Pages
- 2170-2181
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- PMC
- PMC6919635
- OpenAlex
- W2415256624
- Cited by
- 336
- References
- 63
- Citations by year
- 2026: 18 · 2025: 34 · 2024: 28 · 2023: 30 · 2022: 35 · 2021: 48 · 2020: 36 · 2019: 31 · 2018: 37 · 2017: 29 · 2016: 9
- FWCI
- 18.36
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Cell Science (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Health and Medical Research Council (1049968)
- American Heart Association (13BGIA14810010)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- mitochondrial fission, Drp1, Mff, MiD51, Fis1, apoptosis resistance, protein-protein interaction
- MeSH terms
- Gene Editing, Animals, Cell Line, Membrane Proteins, Mice, Inbred C57BL, Staining and Labeling, Cell Death, Receptors, Cytoplasmic and Nuclear, Mice, Knockout, Peroxisomes, Mitochondrial Proteins, Dynamins, Mitochondrial Dynamics
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