A perspective on the determination of mitochondrial biogenesis

Miller, Benjamin F.; Hamilton, Karyn L.

doi:10.1152/ajpendo.00578.2011

Cited by 86Mitochondrial Function and PathologyAdipose Tissue and MetabolismAutophagy in Disease and Therapy

A perspective on the determination of mitochondrial biogenesis

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Miller, Benjamin F., Hamilton, Karyn L.·2012·American Journal of Physiology-Endocrinology and Metabolism

DOI 10.1152/ajpendo.00578.2011PMC PMC3311289

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Abstract

The measurement of mitochondrial biogenesis is important in the determination of aging and disease processes and the assessment of countermeasurements to them. We argue that the frequently used assessments of cell signaling, mitochondrial protein mRNA, mitochondrial protein expression or enzyme activity, and mitochondrial density are not measurements that can lead to conclusions about mitochondrial biogenesis. Instead, we propose that only measurements of mitochondrial synthesis are indicative of biogenesis. Clarification of this issue will hopefully result in further differentiation of the processes important for morphological and functional changes of mitochondria.

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Mitochondrial protein synthesis (MitoPS) is the best measure of mitochondrial biogenesis.
“It has been argued that the best measure of mitochondrial biogenesis is mitochondrial protein synthesis (MitoPS) [48].”
From Exercise mitigates sleep-loss-induced changes in glucose tolerance, mitochondrial function, sarcoplasmic protein synthesis, and diurnal rhythms (2021) · cites this paper as [48]
Mitochondria are dynamic organelles, and the production of new mitochondrial proteins is an important determinant of their overall function.
“Mitochondria are dynamic organelles, and the production of new mitochondrial proteins is an important determinant of their overall function [48].”
From Exercise mitigates sleep-loss-induced changes in glucose tolerance, mitochondrial function, sarcoplasmic protein synthesis, and diurnal rhythms (2021) · cites this paper as [48]
Mitochondrial protein synthesis (MitoPS) is the measure that best reflects the process of mitochondrial biogenesis.
“Mitochondrial protein synthesis (MitoPS) is the measure that best reflects the process of mitochondrial biogenesis [23,48].”
From Exercise mitigates sleep-loss-induced changes in glucose tolerance, mitochondrial function, sarcoplasmic protein synthesis, and diurnal rhythms (2021) · cites this paper as [48]

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Authors: Miller, B. F., & Hamilton, K. L.
Affiliations: Colorado State University (US)
Corresponding author: Miller, Benjamin F.
ORCID: Miller, Benjamin F.
Hamilton, Karyn L.
Venue: American Journal of Physiology-Endocrinology and Metabolism
Publisher: American Physiological Society
Published: 2012-03-01
Volume: 302
Issue: 5
Pages: E496-E499
ISSN: 0193-1849 (ISSN-L)
1522-1555
DOI: 10.1152/ajpendo.00578.2011
PMC: PMC3311289
OpenAlex: W2101661839
Cited by: 86
References: 29
Citations by year: 2026: 2 · 2025: 3 · 2024: 8 · 2023: 6 · 2022: 7 · 2021: 7 · 2020: 12 · 2019: 3 · 2018: 7 · 2017: 6 · 2016: 1 · 2015: 7 · 2014: 6 · 2013: 6 · 2012: 5
FWCI: 2.21
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5k01ag031829-03)
National Institute on Aging (1K01AG031829-01A1)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords: mitochondrial biogenesis, mitochondrial density, mitochondrial protein expression, mitochondrial mRNA, signal transduction, aging, mitochondrial morphology, mitochondrial function
MeSH terms: Aging, Animals, Organelle Biogenesis, DNA, Mitochondrial, Humans, Mitochondria, Models, Biological, Mitochondrial Proteins, Organogenesis, Mitochondrial Membranes

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A perspective on the determination of mitochondrial biogenesis

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