The DOI matches its Crossref record; 83 of the 83 references it cites were found in a registry or an index.
Comprehensive in vivo secondary structure of the SARS-CoV-2 genome reveals novel regulatory motifs and mechanisms
Open accessAbstract
Severe-acute-respiratory-syndrome-related coronavirus 2 (SARS-CoV-2) is the positive-sense RNA virus that causes coronavirus disease 2019 (COVID-19). The genome of SARS-CoV-2 is unique among viral RNAs in its vast potential to form RNA structures, yet as much as 97% of its 30 kilobases have not been structurally explored. Here, we apply a novel long amplicon strategy to determine the secondary structure of the SARS-CoV-2 RNA genome at single-nucleotide resolution in infected cells. Our in-depth structural analysis reveals networks of well-folded RNA structures throughout Orf1ab and reveals aspects of SARS-CoV-2 genome architecture that distinguish it from other RNA viruses. Evolutionary analysis shows that several features of the SARS-CoV-2 genomic structure are conserved across β-coronaviruses, and we pinpoint regions of well-folded RNA structure that merit downstream functional analysis. The native, secondary structure of SARS-CoV-2 presented here is a roadmap that will facilitate focused studies on the viral life cycle, facilitate primer design, and guide the identification of RNA drug targets against COVID-19.
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Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome
Bhatt, P. R., Scaiola, A., Loughran, G., Leibundgut, M., Kratzel, A., Meurs, R., Dreos, R., O’Connor, K. M., McMillan, A., Bode, J. W., et al. (2021) · Science · doi:10.1126/science.abf3546 · Cited by 293
Metadata
- Authors
- Huston, N. C., Wan, H., Strine, M. S., de Cesaris Araujo Tavares, R., Wilen, C. B., & Pyle, A. M.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2021-02
- Volume
- 81
- Issue
- 3
- Pages
- 584-598.e5
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W3116376362
- Cited by
- 316
- References
- 83
- FWCI
- 16.70
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Huston, N. C., Wan, H., Strine, M. S., de Cesaris Araujo Tavares, R., Wilen, C. B., & Pyle, A. M. (2021). Comprehensive in vivo secondary structure of the SARS-CoV-2 genome reveals novel regulatory motifs and mechanisms. Molecular Cell. https://doi.org/10.1016/j.molcel.2020.12.041
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