Cited by 109Lipid metabolism and biosynthesisAlgal biology and biofuel productionMicrobial Metabolic Engineering and Bioproduction

Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae

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Xin, Yi, Shen, Chen, She, Yiting, Chen, Hong, Wang, Cong, Wei, Li, Yoon, Kangsup, Han, Danxiang +2 more·2019·Molecular Plant

Abstract

The composition of polyunsaturated fatty acids (PUFAs) in triacylglycerols (TAGs) is key to health benefits and for oil applications, yet the underlying genetic mechanism remains poorly understood. In this study, by in silico, ex vivo, and in vivo profiling of type-2 diacylglycerol acyltransferases (DGAT2s) in Nannochloropsis oceanica we revealed two novel PUFA-preferring enzymes that discriminate individual PUFA species in TAG assembly, with NoDGAT2J for linoleic acid (LA) and NoDGAT2K for eicosapentaenoic acid (EPA). The LA and EPA composition of TAG molecules is mediated in vivo via the functional partitioning between NoDGAT2J and 2K, both of which are localized in the chloroplast envelope. By modulating transcript abundance of the DGAT2s, an N. oceanica strain bank was created, where proportions of LA and EPA in TAG vary by 18.7-fold (between 0.21% and 3.92% dry weight) and 34.7-fold (between 0.09% and 3.12% dry weight), respectively. These findings lay the foundation for producing designer TAG molecules with tailored health benefits or for biofuel applications in industrial microalgae and higher-plant crops.

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Crossref · 10.1016/j.molp.2018.12.007

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Authors
Xin, Y., Shen, C., She, Y., Chen, H., Wang, C., Wei, L., Yoon, K., Han, D., Hu, Q., & Xu, J.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
Xu, Jian
Venue
Molecular Plant
Publisher
Elsevier BV
Published
2019-04
Volume
12
Issue
4
Pages
474-488
ISSN
1674-2052 (ISSN-L)
OpenAlex
W2905078051
Cited by
108
References
85
Citations by year
2026: 5 · 2025: 10 · 2024: 8 · 2023: 23 · 2022: 14 · 2021: 23 · 2020: 19 · 2019: 6
FWCI
5.18
Type
journal article
Language
en
Open access
Locations
  • Molecular Plant (publishedVersion)
  • PubMed (publishedVersion)
  • Institute of Hydrobiology, CAS Institutional Repository (submittedVersion)
Funders
  • Natural Science Foundation of China (31600059, 31425002, 31741005)
  • Natural Science Foundation of Shandong (ZR2015CQ003, ZR201709180185)
  • Chinese Academy of Sciences (ZDRW-ZS-2016-3)
  • Chinese Key R&D Plan for Synthetic Biology Project
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
triglyceride synthesis, polyunsaturated fatty acids, diacylglycerol acyltransferase, Nannochloropsis oceanica, linoleic acid, eicosapentaenoic acid, chloroplast envelope, fatty acid composition, microalgae, biofuel feedstock
MeSH terms
Fatty Acids, Unsaturated, Industry, Substrate Specificity, Triglycerides, Evolution, Molecular, Protein Transport, Intracellular Space, Diacylglycerol O-Acyltransferase, Gene Knockdown Techniques, Microalgae

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