Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae
Open accessAbstract
The composition of polyunsaturated fatty acids (PUFAs) in triacylglycerols (TAGs) is key to health benefits and for oil applications, yet the underlying genetic mechanism remains poorly understood. In this study, by in silico, ex vivo, and in vivo profiling of type-2 diacylglycerol acyltransferases (DGAT2s) in Nannochloropsis oceanica we revealed two novel PUFA-preferring enzymes that discriminate individual PUFA species in TAG assembly, with NoDGAT2J for linoleic acid (LA) and NoDGAT2K for eicosapentaenoic acid (EPA). The LA and EPA composition of TAG molecules is mediated in vivo via the functional partitioning between NoDGAT2J and 2K, both of which are localized in the chloroplast envelope. By modulating transcript abundance of the DGAT2s, an N. oceanica strain bank was created, where proportions of LA and EPA in TAG vary by 18.7-fold (between 0.21% and 3.92% dry weight) and 34.7-fold (between 0.09% and 3.12% dry weight), respectively. These findings lay the foundation for producing designer TAG molecules with tailored health benefits or for biofuel applications in industrial microalgae and higher-plant crops.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Cites
Hall, T. A. (1999) · Nucleic Acids Symposium Series · doi:10.14601/phytopathol_mediterr-14998u1.29 · Cited by 38,373 · see citing works
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
Tamura, K., Dudley, J. T., Nei, M., & Kumar, S. (2007) · Molecular Biology and Evolution · doi:10.1093/molbev/msm092 · Cited by 28,734 · see citing works
Genome sequence of the palaeopolyploid soybean
Schmutz, J., Cannon, S. B., Schlueter, J. A., Ma, J., Mitros, T., Nelson, W. M., Hyten, D. L., Song, Q., Thelen, J. J., Cheng, J., et al. (2010) · Nature · doi:10.1038/nature08670 · Cited by 4,457 · see citing works
WoLF PSORT: protein localization predictor
Horton, P., Park, K., Obayashi, T., Fujita, N., Harada, H., Adams-Collier, C. J., & Nakai, K. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm259 · Cited by 4,154 · see citing works
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745 · see citing works
Self-citationThe Phaeodactylum genome reveals the evolutionary history of diatom genomes
Bowler, C., Allen, A. E., Badger, J., Grimwood, J., Jabbari, K., Kuo, A., Maheswari, U., Martens, C., Maumus, F., Otillar, R., et al. (2008) · Nature · doi:10.1038/nature07410 · Cited by 1,656 · see citing works
Profiling Membrane Lipids in Plant Stress Responses
Welti, R., Li, W., Li, M., Sang, Y., Biesiada, H., Zhou, H.-E., Rajashekar, C. B., Williams, T. D., & Wang, X. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m205375200 · Cited by 1,128 · see citing works
Nutrition and brain development in early life
Prado, E. L., & Dewey, K. G. (2014) · Nutrition Reviews · doi:10.1111/nure.12102 · Cited by 1,036 · see citing works
Fish Consumption and Risk of Sudden Cardiac Death
Albert, C. M. (1998) · JAMA · doi:10.1001/jama.279.1.23 · Cited by 990 · see citing works
Enzymes of triacylglycerol synthesis and their regulation
Coleman, R. (2003) · Progress in Lipid Research · doi:10.1016/s0163-7827(03)00051-1 · Cited by 961 · see citing works
Derelle, É., Ferraz, C., Rombauts, S., Rouzé, P., Worden, A. Z., Robbens, S., Partensky, F., Degroeve, S., Echeynié, S., Cooke, R., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604795103 · Cited by 879 · see citing works
Omega-3 long-chain polyunsaturated fatty acids and aquaculture in perspective
Tocher, D. R. (2015) · Aquaculture · doi:10.1016/j.aquaculture.2015.01.010 · Cited by 784 · see citing works
Renaud, S. M., Thinh, L.-V., Lambrinidis, G., & Parry, D. L. (2002) · Aquaculture · doi:10.1016/s0044-8486(01)00875-4 · Cited by 660 · see citing works
Storage Lipid Synthesis Is Non-essential in Yeast
Sandager, L., Gustavsson, M. H., Ståhl, U., Dahlqvist, A., Wiberg, E., Banaś, A., Lenman, M., Ronne, H., & Stymne, S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m109109200 · Cited by 538 · see citing works
Sprague, M., Dick, J. R., & Tocher, D. R. (2016) · Scientific Reports · doi:10.1038/srep21892 · Cited by 496 · see citing works
Ajjawi, I., Verruto, J., Aqui, M., Soriaga, L. B., Coppersmith, J. L., Kwok, K., Peach, L., Orchard, E. D., Kalb, R., Xu, W., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3865 · Cited by 472 · see citing works
Vieler, A., Wu, G., Tsai, C.-H., Bullard, B., Cornish, A. J., Harvey, C., Reca, I.-B., Thornburg, C., Achawanantakun, R., Buehl, C. J., et al. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1003064 · Cited by 458 · see citing works
Diacylglycerol, when simplicity becomes complex
Carrasco, S., & Mérida, I. (2006) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2006.11.004 · Cited by 423 · see citing works
Genomic footprints of a cryptic plastid endosymbiosis in diatoms
Moustafa, A., Beszteri, B., Maier, U. G., Bowler, C., Valentin, K., & Bhattacharya, D. (2009) · Science · doi:10.1126/science.1172983 · Cited by 422 · see citing works
Li, J., Han, D., Wang, D., Ning, K., Jia, J., Wei, L., Jing, X., Huang, S., Chen, J., Li, Y., et al. (2014) · The Plant Cell · doi:10.1105/tpc.113.121418 · Cited by 412 · see citing works
Self-citationFatty Acid Desaturases, Polyunsaturated Fatty Acid Regulation, and Biotechnological Advances
Lee, J. M., Lee, H., Kang, S., & Park, W. (2016) · Nutrients · doi:10.3390/nu8010023 · Cited by 401 · see citing works
Lipid Production from Nannochloropsis
Ma, X.-N., Chen, T.-P., Yang, B., Liu, J., & Chen, F. (2016) · Marine Drugs · doi:10.3390/md14040061 · Cited by 394 · see citing works
Phytohormones in microalgae: a new opportunity for microalgal biotechnology?
Lu, Y., & Xu, J. (2015) · Trends in Plant Science · doi:10.1016/j.tplants.2015.01.006 · Cited by 376 · see citing works
Self-citationRuiz‐López, N., Haslam, R. P., Napier, J. A., & Sayanova, O. V. (2013) · The Plant Journal · doi:10.1111/tpj.12378 · Cited by 358 · see citing works
The DGA1 Gene Determines a Second Triglyceride Synthetic Pathway in Yeast
Oelkers, P. M., Cromley, D. A., Padamsee, M., Billheimer, J. T., & Sturley, S. L. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m111646200 · Cited by 336 · see citing works
The plasma membrane–bound phospholipase Dδ enhances freezing tolerance in Arabidopsis thaliana
Li, W., Li, M., Zhang, W., Welti, R., & Wang, X. (2004) · Nature Biotechnology · doi:10.1038/nbt949 · Cited by 330 · see citing works
Diacylglycerol acyltransferase: A key mediator of plant triacylglycerol synthesis
Lung, S., & Weselake, R. J. (2006) · Lipids · doi:10.1007/s11745-006-5057-y · Cited by 327 · see citing works
Kennedy, E. P. (1957) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.26.070157.001003 · Cited by 323 · see citing works
Genome editing of model oleaginous microalgae <i>Nannochloropsis</i> spp. by <scp>CRISPR</scp> /Cas9
Wang, Q., Lu, Y., Xin, Y., Wei, L., Huang, S., & Xu, J. (2016) · The Plant Journal · doi:10.1111/tpj.13307 · Cited by 312 · see citing works
Self-citationFatty Acid and Lipid Transport in Plant Cells
Li, N., Xu, C., Li‐Beisson, Y., & Philippar, K. (2015) · Trends in Plant Science · doi:10.1016/j.tplants.2015.10.011 · Cited by 298 · see citing works
Tonon, T., Harvey, D., Larson, T. R., & Graham, I. A. (2002) · Phytochemistry · doi:10.1016/s0031-9422(02)00201-7 · Cited by 294 · see citing works
Analysis of Amino Acid Motifs Diagnostic for the sn-Glycerol-3-phosphate Acyltransferase Reaction
Lewin, T. M., WANG, P. H., & Coleman, R. (1999) · Biochemistry · doi:10.1021/bi982805d · Cited by 273 · see citing works
Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits
Wang, D., Ning, K., Li, J., Hu, J., Han, D., Wang, H., Zeng, X., Jing, X., Zhou, Q., Su, X., et al. (2014) · PLoS Genetics · doi:10.1371/journal.pgen.1004094 · Cited by 268 · see citing works
Self-citationMembrane Fatty Acid Unsaturation, Protection against Oxidative Stress, and Maximum Life Span
Pamplona, R., Barja, G., & Portero‐Otín, M. (2002) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2002.tb02118.x · Cited by 264 · see citing works
Jia, J., Han, D., Gerken, H. G., Li, Y., Sommerfeld, M. R., Hu, Q., & Xu, J. (2014) · Algal Research · doi:10.1016/j.algal.2014.11.005 · Cited by 239 · see citing works
Self-citationMolecular genetics of yeast :a practical approach
Johnston, J. R. (1994) · Cited by 236 · see citing works
Mühlroth, A., Li, K., Røkke, G. B., Winge, P., Ôlsen, Y., Hohmann‐Marriott, M. F., Vadstein, Ô., & Bones, A. M. (2013) · Marine Drugs · doi:10.3390/md11114662 · Cited by 225 · see citing works
Extensive Functional Diversification of the Populus Glutathione S -Transferase Supergene Family
Lan, T., Yang, Z., Yang, X. N., Liu, Y., Wang, X., & Zeng, Q. (2009) · The Plant Cell · doi:10.1105/tpc.109.070219 · Cited by 224 · see citing works
Poliner, E., Pulman, J. A., Zienkiewicz, K., Childs, K. L., Christoph Benning, A., & Farré, E. M. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12772 · Cited by 169 · see citing works
Effects of CO2 Concentration during Growth on Fatty Acid Composition in Microalgae
Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., & Kawaguchi, A. (1990) · PLANT PHYSIOLOGY · doi:10.1104/pp.93.3.851 · Cited by 164 · see citing works
Wei, L., Wang, Q., Yi, X., Lu, Y., & Xu, J. (2017) · Algal Research · doi:10.1016/j.algal.2017.07.023 · Cited by 159 · see citing works
Self-citationLiu, J., Han, D., Yoon, K., Hu, Q., & Li, Y. (2016) · The Plant Journal · doi:10.1111/tpj.13143 · Cited by 156 · see citing works
Self-citationWei, H., Shi, Y., Ma, X., Pan, Y., Hu, H., Li, Y., Luo, M., Gerken, H. G., & Liu, J. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0858-1 · Cited by 154 · see citing works
Kang, N. K., Jeon, S., Kwon, S., Koh, H. G., Shin, S.-E., Lee, B., Choi, G., Yang, J.-W., Jeong, B., & Chang, Y. K. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0386-9 · Cited by 150 · see citing works
Li, D., Cen, S.-Y., Liu, Y., Balamurugan, S., Zheng, X., Alimujiang, A., Yang, W., Liu, J., & Li, H. (2016) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2016.05.005 · Cited by 149 · see citing works
Yi, X., Lu, Y., Lee, Y., Wei, L., Jia, J., Wang, Q., Wang, D., Bai, F., Hu, H., Hu, Q., et al. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.10.011 · Cited by 148 · see citing works
Self-citationA look at diacylglycerol acyltransferases (DGATs) in algae
Chen, J. E., & Smith, A. G. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.05.009 · Cited by 145 · see citing works
Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.
Fan, J., Ning, K., Zeng, X., Luo, Y., Wang, D., Hu, J., Li, J., Xu, H., Huang, J., Wan, M., et al. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.01174 · Cited by 144 · see citing works
Self-citationTan, W.-J., Yang, Y.-C., Zhou, Y., Huang, L.-P., Xu, L., Chen, Q.-F., Yu, L.-J., & Xiao, S. (2018) · Plant Physiology · doi:10.1104/pp.18.00402 · Cited by 141 · see citing works
Wagner, M., Hoppe, K., Czabany, T., Heilmann, M., Daum, G., Feußner, I., & Fulda, M. S. (2010) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2010.03.008 · Cited by 136 · see citing works
Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane
Corsetto, P. A., Montorfano, G., Zava, S., Jovenitti, I. E., Cremona, A., Berra, B., & Rizzo, A. M. (2011) · Lipids in Health and Disease · doi:10.1186/1476-511x-10-73 · Cited by 135 · see citing works
Guihéneuf, F., & Stengel, D. B. (2013) · Marine Drugs · doi:10.3390/md11114246 · Cited by 131 · see citing works
Mueller, S. P., Unger, M., Guender, L., Fekete, Á., & Mueller, M. J. (2017) · PLANT PHYSIOLOGY · doi:10.1104/pp.17.00861 · Cited by 130 · see citing works
Zhang, Q., Chieu, H. K., Low, C. P., Zhang, S., Heng, C., & Yang, H. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m306998200 · Cited by 126 · see citing works
Lu, Y., Tarkowská, D., Turečková, V., Luo, T., Yi, X., Li, J., Wang, Q., Jiao, N., Strnad, M., & Xu, J. (2014) · The Plant Journal · doi:10.1111/tpj.12615 · Cited by 126 · see citing works
Self-citationZienkiewicz, K., Zienkiewicz, A., Poliner, E., Du, Z., Vollheyde, K., Herrfurth, C., Marmon, S. K., Farré, E. M., Feußner, I., & Christoph Benning, A. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0686-8 · Cited by 126 · see citing works
Gong, Y., Wan, X., Jiang, M., Hu, C., Hu, H., & Huang, F. (2014) · Progress in Lipid Research · doi:10.1016/j.plipres.2014.07.001 · Cited by 124 · see citing works
Membrane fatty acid composition and membrane fluidity as parameters of stress tolerance in yeast
Swan, T. M., & Watson, K. (1997) · Canadian Journal of Microbiology · doi:10.1139/m97-010 · Cited by 117 · see citing works
Beneficial effects of n-3 PUFA on chronic airway inflammatory diseases
Giudetti, A. M., & Cagnazzo, R. (2012) · Prostaglandins & Other Lipid Mediators · doi:10.1016/j.prostaglandins.2012.09.006 · Cited by 109 · see citing works
Mason, R. P., & Sherratt, S. C. R. (2017) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.12.127 · Cited by 108 · see citing works
Cleland, L. G., Caughey, G. E., James, M. J., & Proudman, S. (2006) · PubMed · Cited by 105 · see citing works
Liu, Q., Siloto, R. M. P., Snyder, C. L., & Weselake, R. J. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.204412 · Cited by 105 · see citing works
Ruiz‐López, N., Haslam, R. P., Usher, S. L., Napier, J. A., & Sayanova, O. V. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2013.03.001 · Cited by 104 · see citing works
Lu, Y., Zhou, W., Wei, L., Li, J., Jia, J., Li, F., Smith, S. M., & Xu, J. (2014) · Biotechnology for Biofuels · doi:10.1186/1754-6834-7-81 · Cited by 103 · see citing works
Self-citationSpatial and Temporal Mapping of Key Lipid Species in Brassica napus Seeds
Woodfield, H. K., Sturtevant, D., Borisjuk, L., Munz, E., Guschina, I. A., Chapman, K. D., & Harwood, J. L. (2017) · PLANT PHYSIOLOGY · doi:10.1104/pp.16.01705 · Cited by 101 · see citing works
Verruto, J., Francis, K., Wang, Y., Low, M. C., Greiner, J., Tacke, S., Kuzminov, F. I., Lambert, W., McCarren, J., Ajjawi, I., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1718193115 · Cited by 101 · see citing works
Sanjaya, S., Miller, R., Durrett, T. P., Kosma, D. K., Lydic, T. A., Muthan, B., Koo, A. J., Bukhman, Y. V., Reid, G. E., Howe, G. A., et al. (2013) · The Plant Cell · doi:10.1105/tpc.112.104752 · Cited by 100 · see citing works
Abdukeyum, G. G., Owen, A. J., & McLennan, P. L. (2008) · Journal of Nutrition · doi:10.1093/jn/138.10.1902 · Cited by 93 · see citing works
Guihéneuf, F., Fouqueray, M., Mimouni, V., Ulmann, L., Jacquette, B., & Tremblin, G. (2010) · Journal of Applied Phycology · doi:10.1007/s10811-010-9503-0 · Cited by 92 · see citing works
Trends and strategies to enhance triacylglycerols and high-value compounds in microalgae
Schüler, L. M., Schulze, P. S. C., Pereira, H., Barreira, L., Léon, R., & Varela, J. (2017) · Algal Research · doi:10.1016/j.algal.2017.05.025 · Cited by 89 · see citing works
Diacylglycerol Acyltransferase in Maturing Oil Seeds of Maize and Other Species
Cao, Y.-Z., & Huang, A. H. C. (1986) · PLANT PHYSIOLOGY · doi:10.1104/pp.82.3.813 · Cited by 78 · see citing works
Weselake, R. J., Pomeroy, M. K., Furukawa, T. L., Golden, J. L., Little, D. B., & Laroche, A. (1993) · PLANT PHYSIOLOGY · doi:10.1104/pp.102.2.565 · Cited by 77 · see citing works
Fatty acid composition and tocopherol profiles of safflower (Carthamus tinctorius L.) seed oils
Matthäus, B., Özcan, M. M., & Juhaimi, F. A. (2014) · Natural Product Research · doi:10.1080/14786419.2014.971316 · Cited by 77 · see citing works
Nobusawa, T., Hori, K., Mori, H., Kurokawa, K., & Ohta, H. (2017) · The Plant Journal · doi:10.1111/tpj.13512 · Cited by 72 · see citing works
The plant lipidome in human and environmental health
Horn, P. J., & Christoph Benning, A. (2016) · Science · doi:10.1126/science.aaf6206 · Cited by 67 · see citing works
The Evolution of Algal Chloroplasts
Gibbs, S. P. (1992) · Springer eBooks · doi:10.1007/978-1-4615-2818-0_7 · Cited by 65 · see citing works
de Carvalho, C. C. C. R., Marques, M. P. C., Hachicho, N., & Heipieper, H. J. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-5549-2 · Cited by 60 · see citing works
High efficiency transformation with lithium acetate
Gietz, R. D., & Woods, R. A. (1992) · doi:10.1093/oso/9780199634309.003.0008 · Cited by 60 · see citing works
In vivo Localization Studies in the Stramenopile Alga Nannochloropsis oceanica
Moog, D., Stork, S., Reislöhner, S., Grosche, C., & Maier, U. (2015) · Protist · doi:10.1016/j.protis.2015.01.003 · Cited by 55 · see citing works
Valenzuela, A., Valenzuela, V., Julio Sanhueza, C., & Nieto, S. (2005) · Annals of Nutrition and Metabolism · doi:10.1159/000084177 · Cited by 43 · see citing works
Sukenik, A., Livne, A., Apt, K. E., & Grossman, A. R. (2000) · Journal of Phycology · doi:10.1046/j.1529-8817.2000.99115.x · Cited by 27 · see citing works
Regulation of FATTY ACID ELONGATION1 expression in embryonic and vascular tissues of Brassica napus
Chiron, H., Wilmer, J. A., Lucas, M.-O., Nési, N., Delseny, M., Devic, M., & Roscoe, T. J. (2015) · Plant Molecular Biology · doi:10.1007/s11103-015-0309-y · Cited by 20 · see citing works
The evolution of algal chloroplasts
Gibbs, S. P. (1990) · Experimental phycology · doi:10.1007/978-3-642-48652-4_11 · Cited by 18 · see citing works
Enzymatic synthesis of trieicosapentaenoylglycerol in a solvent-free medium
Borg, P., Binet, C., Girardin, M., Rovel, B., & Barth, D. (2001) · Journal of Molecular Catalysis B Enzymatic · doi:10.1016/s1381-1177(00)00085-0 · Cited by 14 · see citing works
Flagged first, then by citations
1 of the 85 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Patel, A., Karageorgou, D., Rova, E., Katapodis, P., Rova, U., Christakopoulos, P., & Μάτσακας, Λ. (2020) · Microorganisms · doi:10.3390/microorganisms8030434 · Cited by 342 · see citing works
Fatty Acids of Microalgae: Diversity and Applications
Maltsev, Y., & Maltseva, K. (2021) · Reviews in Environmental Science and Bio/Technology · doi:10.1007/s11157-021-09571-3 · Cited by 226 · see citing works
Wang, M., Ye, X., Bi, H., & Shen, Z. (2024) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-024-02461-0 · Cited by 201 · see citing works
Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae
Maltsev, Y., Kulikovskiy, M., & Maltseva, S. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02244-6 · Cited by 174 · see citing works
“Neutral lipids and TAGs are rich in MUFAs, while polar lipids (glycolipids and phospholipids) show a higher percentage of PUFAs [22, 23].”
BackgroundChen, G., Harwood, J. L., Lemieux, M. J., Stone, S. J., & Weselake, R. J. (2022) · Progress in Lipid Research · doi:10.1016/j.plipres.2022.101181 · Cited by 118 · see citing works
Wang, X., Xin, Y., Ren, L., Sun, Z., Zhu, P., Ji, Y., Li, C., Xu, J., & Ma, B. (2020) · Science Advances · doi:10.1126/sciadv.abb3521 · Cited by 113 · see citing works
Self-citationZienkiewicz, A., Zienkiewicz, K., Poliner, E., Pulman, J. A., Du, Z., Stefano, G., Tsai, C., Horn, P. J., Feußner, I., Farré, E. M., et al. (2019) · PLANT PHYSIOLOGY · doi:10.1104/pp.19.00854 · Cited by 101 · see citing works
Wei, L., Hajjami, M. E., Shen, C., You, W., Lu, Y., Li, J., Jing, X., Hu, Q., Zhou, W., Poetsch, A., et al. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1506-8 · Cited by 93 · see citing works
Self-citationKnockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level
Wei, L., Shen, C., Hajjami, M. E., You, W., Wang, Q., Zhang, P., Ji, Y., Hu, H., Hu, Q., Poetsch, A., et al. (2019) · Metabolic Engineering · doi:10.1016/j.ymben.2019.03.004 · Cited by 82 · see citing works
Self-citationEPA and DHA in microalgae: Health benefits, biosynthesis, and metabolic engineering advances
Jesionowska, M., Ovadia, J., Hockemeyer, K., Clews, A. C., & Xu, Y. (2023) · Journal of the American Oil Chemists Society · doi:10.1002/aocs.12718 · Cited by 84 · see citing works
The NanDeSyn database for Nannochloropsis systems and synthetic biology
Gong, Y., Kang, N. K., Kim, Y. U., Wang, Z., Wei, L., Yi, X., Shen, C., Wang, Q., You, W., Lim, J., et al. (2020) · The Plant Journal · doi:10.1111/tpj.15025 · Cited by 74 · see citing works
Self-citationBiochemistry and Biotechnology of Lipid Accumulation in the Microalga Nannochloropsis oceanica
Xu, Y. (2022) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c05309 · Cited by 73 · see citing works
Ye, Y., Liu, M., Yu, L., Sun, H., & Liu, J. (2024) · Marine Drugs · doi:10.3390/md22020054 · Cited by 73 · see citing works
Jakhwal, P., Biswas, J. K., Tiwari, A., Kwon, E. E., & Bhatnagar, A. (2021) · Bioresource Technology · doi:10.1016/j.biortech.2021.126250 · Cited by 70 · see citing works
Zhang, P., Xin, Y., He, Y., Tang, X., Shen, C., Wang, Q., Lv, N., Li, Y., Hu, Q., & Xu, J. (2022) · Nature Communications · doi:10.1038/s41467-022-29337-x · Cited by 67 · see citing works
Self-citationMetabolic and functional connections between cytoplasmic and chloroplast triacylglycerol storage
Xu, C., Fan, J., & Shanklin, J. (2020) · Progress in Lipid Research · doi:10.1016/j.plipres.2020.101069 · Cited by 60 · see citing works
Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives
Song, Y., Wang, F., Chen, L., & Zhang, W. (2024) · Marine Drugs · doi:10.3390/md22050216 · Cited by 58 · see citing works
Wang, S., Lan, C., Wang, Z., Wan, W., Zhang, H., Cui, Q., & Song, X. (2020) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.0c04299 · Cited by 56 · see citing works
Robust Spontaneous Raman Flow Cytometry for Single‐Cell Metabolic Phenome Profiling via pDEP‐DLD‐RFC
Wang, X., Ren, L., Diao, Z., He, Y., Zhang, J., Liu, M., Li, Y., Sun, L., Chen, R., Ji, Y., et al. (2023) · Advanced Science · doi:10.1002/advs.202207497 · Cited by 52 · see citing works
Self-citationLiu, J., Liu, M., Pan, Y., Shi, Y., & Hu, H. (2021) · Metabolic Engineering · doi:10.1016/j.ymben.2021.11.015 · Cited by 47 · see citing works
Su, H., Shi, P., Shen, Z., Meng, H., Meng, Z., Han, X., Chen, Y., Fan, W., Fa, Y., Yang, C., et al. (2023) · Communications Biology · doi:10.1038/s42003-023-05502-w · Cited by 45 · see citing works
You, W., Wei, L., Gong, Y., Hajjami, M. E., Xu, J., & Poetsch, A. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01748-2 · Cited by 45 · see citing works
Self-citationXu, J., Li, T., Li, C.-L., Zhu, S.-N., Wang, Z.-M., & Zeng, E. Y. (2020) · Algal Research · doi:10.1016/j.algal.2020.101910 · Cited by 44 · see citing works
Self-citationCurrent perspectives on integrated approaches to enhance lipid accumulation in microalgae
Rawat, J., Gupta, P. K., Pandit, S., Prasad, R., & Pande, V. (2021) · 3 Biotech · doi:10.1007/s13205-021-02851-3 · Cited by 44 · see citing works
Genome engineering of Nannochloropsis with hundred‐kilobase fragment deletions by Cas9 cleavages
Wang, Q., Gong, Y., He, Y., Yi, X., Lv, N., Du, X., Li, Y., Jeong, B., & Xu, J. (2021) · The Plant Journal · doi:10.1111/tpj.15227 · Cited by 37 · see citing works
Self-citationHayyan, A., Samyudia, A. V., Hashim, M. A., Hizaddin, H. F., Ali, E. T., Hadj-Kali, M. K., Aldeehani, A. K., Alkandari, K. H., Etigany, H. T., Alajmi, F. D., et al. (2021) · Industrial Crops and Products · doi:10.1016/j.indcrop.2021.114242 · Cited by 37 · see citing works
PDAT regulates PE as transient carbon sink alternative to triacylglycerol in Nannochloropsis
Yang, J., Liu, J., Pan, Y., Maréchal, É., Amato, A., Liu, M., Gong, Y., Li, Y., & Hu, H. (2022) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiac160 · Cited by 36 · see citing works
Wang, Q., Feng, Y., Lu, Y., Yi, X., Shen, C., Wei, L., Liu, Y., Lv, N., Du, X., Zhu, W., et al. (2021) · Metabolic Engineering · doi:10.1016/j.ymben.2021.03.015 · Cited by 34 · see citing works
Self-citationBiotechnology in Future Food Lipids: Opportunities and Challenges
Xu, Q., Tang, Q., Xu, Y., Wu, J., Mao, X., Li, F., Wang, S., & Wang, Y. (2023) · Annual Review of Food Science and Technology · doi:10.1146/annurev-food-060721-024353 · Cited by 34 · see citing works
Mavrommatis, A., Skliros, D., Flemetakis, E., & Tsiplakou, E. (2021) · Microorganisms · doi:10.3390/microorganisms9071528 · Cited by 33 · see citing works
Ma, H., Wu, X., Wei, Z.-W., Zhao, L., Li, Z., Liang, Q. D., Zheng, J., Wang, Y., Li, Y., Huang, L., et al. (2020) · Journal of Experimental Botany · doi:10.1093/jxb/eraa451 · Cited by 29 · see citing works
Self-citationStructural insight into a GH1 β-glucosidase from the oleaginous microalga, Nannochloropsis oceanica
Dong, S., Liu, Y., Zhou, H., Xiao, Y., Xu, J., Cui, Q., Wang, X., & Feng, Y. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.12.128 · Cited by 29 · see citing works
Self-citationRecent advances in biotechnology for marine enzymes and molecules
Zhang, J., Jiang, L., Chen, X., Lv, K., Basiony, M., Zhu, G., Karthik, L., Ouyang, L., Zhang, L., & Liu, X. (2021) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2021.05.009 · Cited by 27 · see citing works
Lan, C., Wang, S., Zhang, H., Wang, Z., Wan, W., Liu, H., Hu, Y., Cui, Q., & Song, X. (2021) · Biotechnology for Biofuels · doi:10.1186/s13068-021-02096-5 · Cited by 26 · see citing works
Xu, Y., Caldo, K. M. P., Falarz, L. J., Jayawardhane, K. N., & Chen, G. (2020) · The Plant Journal · doi:10.1111/tpj.14708 · Cited by 25 · see citing works
Yang, M., Kong, F., Xie, X., Wu, P., Chu, Y., Cao, X., & Xue, S. (2020) · Plant and Cell Physiology · doi:10.1093/pcp/pcaa012 · Cited by 25 · see citing works
Ragozzino, C., Casella, V., Coppola, A., Scarpato, S., Buonocore, C., Consiglio, A., Esposito, F. P., Galasso, C., Tedesco, P., Sala, G. D., et al. (2025) · Marine Drugs · doi:10.3390/md23030116 · Cited by 26 · see citing works
Young, D. Y., Pang, N., & Shachar‐Hill, Y. (2022) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiac154 · Cited by 24 · see citing works
Zhou, Y., Huang, X., Hu, T., Chen, S.-W., Wang, Y., Shi, X., Yin, M., Li, R., Wang, J., & Jia, X. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms242015106 · Cited by 21 · see citing works
Long, J., Jia, J., Gong, Y., Han, D., & Hu, Q. (2022) · Marine Drugs · doi:10.3390/md20060343 · Cited by 21 · see citing works
Self-citationHadrová, S., Sedláková, K., Křížová, L., & Malyugina, S. (2021) · Animals · doi:10.3390/ani11061817 · Cited by 20 · see citing works
Chen, Z., He, M., Wang, H., Li, X., Qin, R., Ding, Y., Zhai, X., Zhu, J., Zhang, Q., Hu, P., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-54258-2 · Cited by 17 · see citing works
Lv, M., Wang, F., Zeng, L., Bi, Y., Cui, J., Liu, L., Bi, Y., Chen, L., & Zhang, W. (2020) · Algal Research · doi:10.1016/j.algal.2020.102001 · Cited by 17 · see citing works
Vitali, L., Lolli, V., Sansone, F., Kumar, A., Concas, A., & Lutzu, G. A. (2023) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-023-04153-5 · Cited by 16 · see citing works
Gao, B., Xu, M., Shan, D., Zhang, C., Yang, Y., Dong, Z., Zhang, H., Han, B., Huang, L., & Zhang, C. (2023) · BMC Biology · doi:10.1186/s12915-023-01618-x · Cited by 15 · see citing works
Do, S., & Du, Z. (2024) · Energies · doi:10.3390/en17205218 · Cited by 15 · see citing works
Cui, H., Xu, W., Zhu, X., Zhao, C., Cui, Y., Ji, C., Zhang, C., Xue, J., Qin, S., Jia, X., et al. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.771300 · Cited by 15 · see citing works
Perlikowski, D., Lechowicz, K., Skirycz, A., Michaelis, Ä., Pawłowicz, I., & Kosmala, A. (2022) · Plant and Cell Physiology · doi:10.1093/pcp/pcac003 · Cited by 14 · see citing works
Wang, R., & Miao, X. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.987354 · Cited by 14 · see citing works
Gong, Y., Wang, Q., Wei, L., Liang, W., Wang, L., Lv, N., Du, X., Zhang, J., Shen, C., Yi, X., et al. (2023) · Plant Communications · doi:10.1016/j.xplc.2023.100773 · Cited by 14 · see citing works
Self-citationBagchi, S. K., Patnaik, R., Rawat, I., & Bux, F. (2025) · Bioresource Technology · doi:10.1016/j.biortech.2025.133176 · Cited by 14 · see citing works
Miao, C., Du, M., Du, H., Xu, T., Wu, S., Huang, X., Chen, X., Lei, S., & Xin, Y. (2024) · Marine Drugs · doi:10.3390/md22120570 · Cited by 13 · see citing works
Self-citationFattore, N., Bucci, F., Bellan, A., Bossi, S., Maffei, M. E., & Morosinotto, T. (2022) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2022.07.007 · Cited by 13 · see citing works
Phylogeny and lipid profiles of snow-algae isolated from Norwegian red-snow microbiomes
Suzuki, H., Détain, A., Park, Y., Viswanath, K., Wijffels, R. H., Leborgne‐Castel, N., Procházková, L., & Hulatt, C. J. (2023) · FEMS Microbiology Ecology · doi:10.1093/femsec/fiad057 · Cited by 12 · see citing works
Novel insights into type 2 diacylglycerol acyltransferases in microalga Myrmecia incisa
Liu, W., Sun, Z., Chen, C., Liu, J.-G., & Zhou, Z. (2020) · Journal of Applied Phycology · doi:10.1007/s10811-020-02071-x · Cited by 12 · see citing works
Li, T., Li, C., Wang, W., Wu, H., Wu, H., Xu, J. H., & Xiang, W. (2024) · Marine Drugs · doi:10.3390/md22020082 · Cited by 10 · see citing works
Self-citationKarpagam, R., Jawaharraj, K., Ashokkumar, B., Pugazhendhi, A., & Varalakshmi, P. (2022) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2022.06.002 · Cited by 10 · see citing works
Xin, Y., Wang, Q., Shen, C., Hu, C., Shi, X., Lv, N., Du, X., Xu, G., & Xu, J. (2022) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiac396 · Cited by 9 · see citing works
Self-citationAlgal lipids: structural diversity, analysis and applications
Lopes, D., Aveiro, S. S., Conde, T., Rey, F., Couto, D., Melo, T., Moreira, A. S. P., & Domingues, M. R. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-98819-3.00002-x · Cited by 9 · see citing works
Characterization of CiWRI1 from Carya illinoinensis in Seed Oil Biosynthesis
Zhou, X., Dai, Y., Wu, H., Zhong, P., Luo, L., Shang, Y., Tan, P., Peng, F., & Tian, Z. (2020) · Forests · doi:10.3390/f11080818 · Cited by 9 · see citing works
Wang, T., Wang, F., Zeng, L., Guo, P., Wu, Y., Chen, L., & Zhang, W. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1106265 · Cited by 8 · see citing works
Introduction to Essential Fatty Acids
Patel, A., Rova, U., Christakopoulos, P., & Μάτσακας, Λ. (2020) · Wiley eBooks · doi:10.1002/9781119631729.ch1 · Cited by 9 · see citing works
Functions and substrate selectivity of diacylglycerol acyltransferases from Mortierella alpina
Li, J., Chen, H., Chang, L., Wu, C., Zhang, H., Chen, Y. Q., & Chen, W. (2023) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-023-12694-y · Cited by 7 · see citing works
Self-citationLiang, S., Zhang, Z., Liu, H., Guo, L., Sun, S., & Yang, G. (2019) · Journal of Oceanology and Limnology · doi:10.1007/s00343-019-9189-3 · Cited by 7 · see citing works
Vitali, L., Lolli, V., Sansone, F., Concas, A., & Lutzu, G. A. (2022) · BioEnergy Research · doi:10.1007/s12155-022-10534-x · Cited by 7 · see citing works
Zhou, Y., Huang, X., Sun, Y., Chai, X., Wen, J., Yang, Z., Xue, J., Zhang, X., Jia, X., Wang, J., et al. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c06976 · Cited by 6 · see citing works
Functional characterization of two diacylglycerol acyltransferase 1 genes in Mortierella alpina
Li, J., Cao, J., Chen, H., Tang, X., Zhang, H., & Chen, W. (2021) · Letters in Applied Microbiology · doi:10.1111/lam.13597 · Cited by 6 · see citing works
Self-citationLiu, K., Li, J., Xing, C., Yuan, H., & Yang, J. (2023) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-023-02318-y · Cited by 6 · see citing works
State-of-art engineering approaches for ameliorated production of microbial lipid
Mondal, S., Halder, S. K., & Mondal, K. C. (2023) · Systems Microbiology and Biomanufacturing · doi:10.1007/s43393-023-00195-y · Cited by 6 · see citing works
Singh, R. P., Singh, P. K., Kumar, I., Kumar, M., Gaur, V. K., Kaushik, A., Arya, A., Saikia, M., Santos‐Villalobos, S. D. L., Kumar, A., et al. (2026) · Energy Strategy Reviews · doi:10.1016/j.esr.2026.102050 · Cited by 5 · see citing works
Hao, X., Hu, F., Pan, Y., Yin, W., & Hu, H. (2024) · Algal Research · doi:10.1016/j.algal.2024.103497 · Cited by 5 · see citing works
Harnessing microalgae for bioproducts: innovations in synthetic biology
Li, Z., Cheng, Y., Li, C., Wu, Q., & Xin, Y. (2025) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-025-04727-7 · Cited by 4 · see citing works
Self-citationWu, S., She, Y., Chen, X., Huang, X., Xu, T., Miao, C., Wu, Q., Li, Z., Li, C., Cheng, Y., et al. (2025) · Plant Cell Reports · doi:10.1007/s00299-025-03511-z · Cited by 4 · see citing works
Self-citationZheng, J., Liu, Y., Ge, F., Zhang, C., Han, D., & Ma, H. (2025) · Algal Research · doi:10.1016/j.algal.2025.104261 · Cited by 4 · see citing works
Self-citationYou, W., Xu, C., Zhang, J., & Poetsch, A. (2025) · Biology · doi:10.3390/biology14111599 · Cited by 4 · see citing works
Ma, H., Zheng, J., Li, Y., Zhao, L., Zou, S., Hu, Q., & Han, D. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.794714 · Cited by 5 · see citing works
Self-citationFatty acid composition and distribution in triacylglycerols of cultured fishes in Japan
Mochizuki, M., Matsuyama, H., Suzuki, K., & Watanabe, Y. (2020) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2020.101687 · Cited by 4 · see citing works
Microalgae: A Treasure House for Polyunsaturated Fatty Acid (PUFA) Production
Sharma, N., Sharma, S. G., Kocher, G. S., & Dhir, A. (2025) · National Academy Science Letters · doi:10.1007/s40009-025-01867-0 · Cited by 3 · see citing works
Wang, R., Li, J., Zhang, F., & Miao, X. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c05511 · Cited by 3 · see citing works
Xu, T., Jiang, X., Chen, X., Li, Z., Miao, C., Wu, S., Cheng, Y., Li, C., Xin, Y., & Lu, Y. (2025) · Aquaculture · doi:10.1016/j.aquaculture.2025.743471 · Cited by 2 · see citing works
Self-citationWang, Q., Gong, Y., Wang, L., Lv, N., Du, X., Zhang, J., Xin, Y., Nikoloski, Z., Li‐Beisson, Y., Sun, L., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-74304-5 · Cited by 2 · see citing works
Self-citationYang, R., Wang, H., Zhu, L., Zhu, L., Liu, T., & Zhang, D. (2023) · The Journal of Microbiology · doi:10.1007/s12275-022-00001-9 · Cited by 2 · see citing works
Phukan, M. M., Kumar, R., Gupta, K., Bardhan, P., Bhuyan, N., Gogoi, L., Bora, P., Mandal, M., & Kataki, R. (2020) · Springer eBooks · doi:10.1007/978-981-15-7493-1_7 · Cited by 2 · see citing works
Yang, X., & Cui, X. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.03.004 · Cited by 1 · see citing works
Genome engineering of Nannochloropsis with large deletions for constructing microalgal minigenomesPreprint
Wang, Q., Gong, Y., He, Y., Xin, Y., Lv, N., Du, X., Li, Y., Jeong, B., & Xu, J. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.10.08.332478 · Cited by 1 · see citing works
Self-citationSelçuk, E., & Çakmak, T. (2019) · Acta Aquatica Turcica · doi:10.22392/actaquatr.508780
Vitali, L., Lolli, V., Sansone, F., Concas, A., & Lutzu, G. A. (2022) · Research Square · doi:10.21203/rs.3.rs-1797363/v1
Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina
Yu, R., Chang, L., Cao, J., Yang, B. K., Chen, H., & Chen, W. (2023) · Journal of Fungi · doi:10.3390/jof9020219
Self-citationSu, H., Shi, P., Shen, Z., Meng, H., Men, Z., Han, X., Chen, Y., Fan, W., Fa, Y., Yang, C., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.03.28.534371
Protein Engineering in Cyanobacterial Biotechnology: Tools and Recent Updates
Tyagi, S., Kar, S., Srivastava, A., & Shukla, P. (2023) · Current Protein and Peptide Science · doi:10.2174/1389203724666230822100104
Gao, B., Xu, M., Shan, D., Zhang, C., Yang, Y., Dong, Z., Zhang, H., Han, B., Huang, L., & Zhang, C. (2023) · Figshare · doi:10.6084/m9.figshare.23305124
Gao, B., Xu, M., Shan, D., Zhang, C., Yang, Y., Dong, Z., Zhang, H., Han, B., Huang, L., & Zhang, C. (2023) · Figshare · doi:10.6084/m9.figshare.23305118
Hu, Z., Hao, X., Pan, Y., & Hu, H. (2025) · Algal Research · doi:10.1016/j.algal.2025.103902
Lan, C., Wang, S., Zhang, H., Wang, Z., Wan, W., Liu, H., Hu, Y., Cui, Q., & Song, X. (2021) · Figshare · doi:10.6084/m9.figshare.17696356
Lan, C., Wang, S., Zhang, H., Wang, Z., Wan, W., Liu, H., Hu, Y., Cui, Q., & Song, X. (2021) · Figshare · doi:10.6084/m9.figshare.17696353
Liu, K., Li, J., Xing, C., Yuan, H., & Yang, J. (2023) · Figshare · doi:10.6084/m9.figshare.22678244
Lan, C., Wang, S., Zhang, H., Wang, Z., Wan, W., Liu, H., Hu, Y., Cui, Q., & Song, X. (2021) · Figshare · doi:10.6084/m9.figshare.17696365
Lan, C., Wang, S., Zhang, H., Wang, Z., Wan, W., Liu, H., Hu, Y., Cui, Q., & Song, X. (2021) · Figshare · doi:10.6084/m9.figshare.17696362
Lan, C., Wang, S., Zhang, H., Wang, Z., Wan, W., Liu, H., Hu, Y., Cui, Q., & Song, X. (2021) · Figshare · doi:10.6084/m9.figshare.17696368
- Authors
- Xin, Y., Shen, C., She, Y., Chen, H., Wang, C., Wei, L., Yoon, K., Han, D., Hu, Q., & Xu, J.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Xu, Jian
- Venue
- Molecular Plant
- Publisher
- Elsevier BV
- Published
- 2019-04
- Volume
- 12
- Issue
- 4
- Pages
- 474-488
- ISSN
- 1674-2052 (ISSN-L)
- OpenAlex
- W2905078051
- Cited by
- 108
- References
- 85
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 8 · 2023: 23 · 2022: 14 · 2021: 23 · 2020: 19 · 2019: 6
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Plant (publishedVersion)
- PubMed (publishedVersion)
- Institute of Hydrobiology, CAS Institutional Repository (submittedVersion)
- Funders
- Natural Science Foundation of China (31600059, 31425002, 31741005)
- Natural Science Foundation of Shandong (ZR2015CQ003, ZR201709180185)
- Chinese Academy of Sciences (ZDRW-ZS-2016-3)
- Chinese Key R&D Plan for Synthetic Biology Project
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- triglyceride synthesis, polyunsaturated fatty acids, diacylglycerol acyltransferase, Nannochloropsis oceanica, linoleic acid, eicosapentaenoic acid, chloroplast envelope, fatty acid composition, microalgae, biofuel feedstock
- MeSH terms
- Fatty Acids, Unsaturated, Industry, Substrate Specificity, Triglycerides, Evolution, Molecular, Protein Transport, Intracellular Space, Diacylglycerol O-Acyltransferase, Gene Knockdown Techniques, Microalgae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review