Vascular endothelial growth factor a modified mRNA engineered cellular electrospun membrane complexes promotes mouse skin wound repair
Open accessAbstract
Artificial skin substitutes are one of the most promising areas of wound healing research; however, graft survival largely depends on how the treatment is performed. Early angiogenesis is essential for wound healing and graft survival and vascular endothelial growth factor A (VEGFA) is an important cytokine that stimulates angiogenesis. Here, we first investigated the effects of different ratios of collagen (BC) and gelatin blended with poly (l-lactide-co-caprolactone) (PLCL) on nanofibrous membranes. The Young's modulus and cell proliferation were significantly higher in the 50% BC group than that in all other groups. Then, cellular electrospun membrane complexes (CEMC) were successfully constructed from nanoscaffolds and fibroblasts extracted from human foreskin and engineered with controlled autocrine VEGFA by transfecting VEGFA modified mRNA (modRNA). Engineered CEMC significantly promoted wound healing in vivo and contributed to stable vascular network formation in the grafted area, thereby increasing the survival rate of the engineered skin. This study provides a potential solution for wound healing while establishing the value of different RNA modification methods for various engineered skins in the future, thereby advancing engineered skin development.
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Growth factors such as FGF and VEGF incorporated into or onto the PLCL matrix promote migration, adhesion, and proliferation of fibroblasts and vascular regeneration in the wound area, accelerating healing.
“Furthermore, growth factors such as FGF and VEGF can be incorporated into or onto the PLCL matrix during fabrication. This promotes the migration, adhesion, and proliferation of fibroblasts, as well as vascular regeneration in the wound area, thereby accelerating the healing process [146].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [146]
The Results (Biocompatibility of BC/GEL/PLCL nanofibers; page not provided) report fibroblast adhesion and proliferation on the scaffold. The Results (modVEGFA treatment promotes CEMC vascularization and survival; page not provided) report greater vascularization with VEGFA-engineered cells. The paper does not show FGF or VEGF incorporated into the PLCL matrix during fabrication, or growth-factor-driven fibroblast migration; VEGFA was produced by transfected fibroblasts. (LLM, conf=0.88)
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Application of PLCL as a biodegradable polymer in biomedical engineering
Zhao, Y., & Liu, H. (2026) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-026-07016-3 · Cited by 7 · see citing works
- Authors
- Ai, X., Luo, R., Wang, H., Yan, B., Li, K., Yu, X., Dong, W., Tan, Y., Liu, M., Chen, Y., Lu, T., Wang, X., Wang, W., & Fu, W.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Corresponding author
- Wang, Wei
- Fu, Wei
- ORCID
- Venue
- Materials Today Bio
- Publisher
- Elsevier BV
- Published
- 2023-10
- Volume
- 22
- Pages
- 100776
- ISSN
- 2590-0064 (ISSN-L)
- PMC
- PMC10474086
- OpenAlex
- W4386102229
- Cited by
- 14
- References
- 47
- Citations by year
- 2026: 5 · 2024: 8 · 2023: 1
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Materials Today Bio (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Natural Science Foundation of Shanghai Municipality (20ZR1434500)
- Shanghai Science and Technology Development Foundation (PKJ2020-Y06)
- National Natural Science Foundation of China (82070430, 82272178)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- VEGF-A, engineered skin substitutes, wound healing, angiogenesis, electrospun membranes, poly(L-lactide-co-ε-caprolactone), autologous fibroblasts, skin graft survival, vascular network formation, Young's modulus, cell proliferation
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