Visualizing the Cortical Representation of Whisker Touch: Voltage-Sensitive Dye Imaging in Freely Moving Mice
Open accessAbstract
Voltage-sensitive dye imaging resolves the spatiotemporal dynamics of supragranular subthreshold cortical activity with millisecond temporal resolution and subcolumnar spatial resolution. We used a flexible fiber optic image bundle to visualize voltage-sensitive dye dynamics in the barrel cortex of freely moving mice while simultaneously filming whisker-related behavior to generate two movies matched frame-by-frame with a temporal resolution of up to 2 ms. Sensory responses evoked by passive whisker stimulation lasted longer and spread further across the barrel cortex in awake mice compared to anesthetized mice. Passively evoked sensory responses were large during behaviorally quiet periods and small during active whisking. However, as an exploring mouse approached an object while whisking, large-amplitude, propagating cortical sensory activity was evoked by active whisker-touch. These experiments demonstrate that fiber optics can be used to image cortical sensory activity with high resolution in freely moving animals. The results demonstrate differential processing of sensory input depending upon behavior.
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In two animals using voltage sensitive dyes, somatosensory cortex function was present 40-90 min after reperfusion.
“To further examine this issue, in two animals using voltage sensitive dyes that directly measure sensory-evoked depolarization (Grinvald and Hildesheim, 2004;Ferezou et al., 2006), we confirmed that somatosensory cortex function was present 40 -90 min after reperfusion (supplemental Fig. 2, available at www.jneurosci.org as supplemental material).”
From Two-Photon Imaging of Stroke Onset<i>In Vivo</i>Reveals That NMDA-Receptor Independent Ischemic Depolarization Is the Major Cause of Rapid Reversible Damage to Dendrites and Spines (2008) · cites this paper as Ferezou et al., 2006
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“…dyes (Shoham et al., 1999; Grinvald and Hildesheim, 2004) have allowed routine measurement of the spatiotemporal dynamics of sensory processing in head-fixed animals (Spors and Grinvald, 2002; Seidemann et al., 2002; Slovin et al., 2002; Petersen et al., 2003a, 2003b; Derdikman et al., 2003).”
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- Authors
- Ferezou, I., Bolea, S., & Petersen, C. C. H.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Corresponding author
- Petersen, Carl C.H.
- ORCID
- Petersen, Carl C.H.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2006-05
- Volume
- 50
- Issue
- 4
- Pages
- 617-629
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2099044759
- Cited by
- 466
- References
- 2
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 14 · 2023: 16 · 2022: 14 · 2021: 12 · 2020: 20 · 2019: 22 · 2018: 35 · 2017: 29 · 2016: 25 · 2015: 32 · 2014: 26 · 2013: 27 · 2012: 34
- FWCI
- 15.15
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 2670 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Fondation Leenaards
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Life Sciences › Neuroscience › Cognitive Neuroscience › Tactile and Sensory Interactions
- Keywords
- barrel cortex, voltage-sensitive dye imaging, freely moving mice, fiber-optic imaging
- MeSH terms
- Anesthesia, General, Animals, Behavior, Animal, Brain Mapping, Diagnostic Imaging, Evoked Potentials, Somatosensory, Fiber Optic Technology, Fluorescent Dyes, Image Processing, Computer-Assisted, Somatosensory Cortex, Vibrissae, Wakefulness, Patch-Clamp Techniques, Mice, Optical Fibers
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