Patterns of mRNA and protein expression for 12 GABAA receptor subunits in the mouse brain
Open accessAbstract
The GABAA receptor is the main inhibitory receptor in the brain and its subunits originate from different genes or gene families (α1-α6, β1-β3, γ1-γ3, δ, ε, θ, π, or ρ1-3). In the mouse brain the anatomical distribution of GABAA receptor subunit mRNAs so far investigated is restricted to subunits forming benzodiazepine-sensitive receptor complexes (α1-α3, α5, β2, β3 and γ2) in the forebrain and midbrain as assessed by in situ hybridization (ISH). In the present study the anatomical distribution of the GABAA receptor subunits α1-α6, β1-β3, γ1-γ2 and δ was analyzed in the mouse brain (excluding brain stem) by ISH and immunohistochemistry (IHC). In several brain areas such as hippocampus, cerebellum, bulbus olfactorius and habenula we observed that mRNA levels did not reflect protein levels, indicating that the protein is located far distantly from the cell body. We also compared the distribution of these 12 subunit mRNAs and proteins with that reported in the rat brain. Although in general there is a considerable correspondence in the distribution between mouse and rat brains, several species-specific differences were observed.
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α5GABAARs are expressed at negligible levels in the CeA.
“This finding is striking, as α5GABAARs are expressed at negligible levels in the CeA [35, 36]”
From An emerging circuit pharmacology of GABAA receptors (2018) · cites this paper as [36]
α2GABAARs are densely expressed in the central amygdala.
“α2-GABAARs are densely expressed in the central amygdala [35, 36, 40], but their precise cellular location and function are still unknown.”
From An emerging circuit pharmacology of GABAA receptors (2018) · cites this paper as [36]
α1, α2, α3, α4 and α5GABAARs are all expressed at moderate to high levels in the hippocampus.
“α1, α2, α3, α4 and α5GABAARs are all expressed at moderate to high levels in the hippocampus (although at different relative levels in different subregions; [36])”
From An emerging circuit pharmacology of GABAA receptors (2018) · cites this paper as [36]
α5GABAARs are expressed in the olfactory bulb, cortex, hippocampus, and granule cells within the cerebellum.
“The potential α5-mediated circuitry involved in stress responsiveness can begin to be teased apart based on the localized expression pattern of α5GABAARs in the olfactory bulb, cortex, hippocampus, and granule cells within the cerebellum [36].”
From An emerging circuit pharmacology of GABAA receptors (2018) · cites this paper as [36]
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- Authors
- Hörtnagl, H., Tasan, R. O., Wieselthaler, A., Kirchmair, E., Sieghart, W., & Sperk, G.
- Affiliations
- Innsbruck Medical University (AT)
- Medical University of Vienna (AT)
- Corresponding author
- Hörtnagl, H.
- Sperk, G.
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2013-04
- Volume
- 236
- Pages
- 345-372
- ISSN
- 0306-4522 (ISSN-L)
- PMC
- PMC3605588
- OpenAlex
- W2078682888
- Cited by
- 229
- References
- 45
- Citations by year
- 2026: 11 · 2025: 7 · 2024: 15 · 2023: 22 · 2022: 16 · 2021: 24 · 2020: 18 · 2019: 15 · 2018: 21 · 2017: 16 · 2016: 15 · 2015: 14 · 2014: 26 · 2013: 8
- FWCI
- 10.31
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Neuroscience (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Austrian Science Fund
- S10204
- P19464
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- GABAA receptor, receptor subunits, mRNA expression, protein expression, anatomical distribution, mouse brain, hippocampus, cerebellum, habenula, subcellular localization, species differences
- MeSH terms
- Animals, Brain, Immunohistochemistry, Male, Mice, Inbred C57BL, Receptors, GABA-A, RNA, Messenger, Species Specificity, In Situ Hybridization, Mice, Rats
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