Neonatal genistein or bisphenol-A exposure alters sexual differentiation of the AVPV
Abstract
There is growing concern that naturally occurring and chemically manufactured endocrine-active compounds (EACs) may disrupt hormone-dependent events during central nervous system development. We examined whether postnatal exposure to the phytoestrogen genistein (GEN) or the plastics component bisphenol-A (BIS) affected sexual differentiation of the anteroventral periventricular nucleus of the hypothalamus (AVPV) in rats. The AVPV is sexually differentiated in rodents. The female AVPV is larger than the male AVPV and contains a higher number of cells expressing tyrosine hydroxylase (TH). Sexual differentiation of the AVPV results from exposure of the male nervous system to estrogen aromatized from testicular testosterone secreted in the first few days after birth. Thus, we hypothesized that exposure to EACs during this critical period could alter the sexually dimorphic expression of TH and the overall expression of estrogen receptor alpha (ERalpha) in the AVPV. Animals were given 4 subcutaneous injections of sesame oil (control), 50 microg 17beta-estradiol (E2), 250 microg GEN, or 250 microg BIS at 12-h intervals over postnatal days (PND) 1 and 2 and sacrificed on PND 19. E2 treatment masculinized TH immunoreactivity (TH-ir) in the female AVPV while exposure to GEN or BIS demasculinized TH-ir in the male AVPV. In addition, we identified a population of neurons co-expressing TH and ERalpha located primarily in the medial region of the AVPV. Normally, females have nearly three t…
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Neonatal treatment with BPA reduces sex differences in the brain and can modify neurite and dendrite formation in rodents.
“Neonatal treatment with BPA in general reduces sex differences in the brain and can modify neurite and dendrite formation [3], [8], [9], [10], [11].”
From Gestational Exposure to Low Dose Bisphenol A Alters Social Behavior in Juvenile Mice (2011) · cites this paper as [3]
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Distribution of estrogen receptor alpha and beta immunoreactive profiles in the postnatal rat brain
Pérez, S. E., Chen, E.-Y., & Mufson, E. J. (2003) · Developmental Brain Research · doi:10.1016/s0165-3806(03)00223-2 · Cited by 228 · see citing works
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Hormones and endocrine-disrupting chemicals: low-dose effects and nonmonotonic dose responses
Vandenberg, L. N., Colborn, T., Hayes, T. B., Heindel, J. J., Jacobs, D. R., Lee, D.-H., Shioda, T., Soto, A. M., vom Saal, F. S., Welshons, W. V., et al. (2012) · Endocrine Reviews · doi:10.1210/er.2011-1050 · Cited by 3,209 · see citing works
Estradiol and the Developing Brain
McCARTHY, M. M. (2008) · Physiological Reviews · doi:10.1152/physrev.00010.2007 · Cited by 885 · see citing works
Kundakovic, M., Gudsnuk, K., Franks, B., Madrid, J., Miller, R. L., Perera, F. P., & Champagne, F. A. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1214056110 · Cited by 490 · see citing works
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Wolstenholme, J. T., Rissman, E. F., & Connelly, J. J. (2011) · Hormones and Behavior · doi:10.1016/j.yhbeh.2010.10.001 · Cited by 309 · see citing works
Gestational Exposure to Low Dose Bisphenol A Alters Social Behavior in Juvenile Mice
Wolstenholme, J. T., Taylor, J. A., Shetty, S. R. J., Edwards, M., Connelly, J. J., & Rissman, E. F. (2011) · PLoS ONE · doi:10.1371/journal.pone.0025448 · Cited by 168 · see citing works
“Several countries have banned BPA in production of new products based on findings from animal studies that suggest BPA can affect the development of prostate, brain and behavior [3,4,5,6,7].”
BackgroundCao, J., Rebuli, M. E., Rogers, J., Todd, K. L., Leyrer, S. M., Ferguson, S. A., & Patisaul, H. B. (2013) · Toxicological Sciences · doi:10.1093/toxsci/kft035 · Cited by 141 · see citing works
Self-citationArambula, S. E., Belcher, S. M., Planchart, A., Turner, S. D., & Patisaul, H. B. (2016) · Endocrinology · doi:10.1210/en.2016-1339 · Cited by 87 · see citing works
Self-citationAdewale, H. B., Todd, K. L., Mickens, J. A., & Patisaul, H. B. (2011) · NeuroToxicology · doi:10.1016/j.neuro.2010.07.008 · Cited by 86 · see citing works
Self-citationGoldsby, J. A., Wolstenholme, J. T., & Rissman, E. F. (2017) · Endocrinology · doi:10.1210/en.2016-1188 · Cited by 48 · see citing works
Viglietti-Panzica, C., Mura, E., & Panzica, G. (2007) · Hormones and Behavior · doi:10.1016/j.yhbeh.2006.12.003 · Cited by 37 · see citing works
Neonatal agonism of ERβ impairs male reproductive behavior and attractiveness
Sullivan, A. W., Hamilton, P., & Patisaul, H. B. (2011) · Hormones and Behavior · doi:10.1016/j.yhbeh.2011.04.006 · Cited by 19 · see citing works
Self-citation
- Authors
- Patisaul, H. B., Fortino, A. E., & Polston, E. K.
- Corresponding author
- Polston, Eva K.
- ORCID
- Patisaul, Heather B.
- Venue
- Neurotoxicology and Teratology
- Publisher
- Elsevier BV
- Published
- 2006-01
- Volume
- 28
- Issue
- 1
- Pages
- 111-118
- ISSN
- 0892-0362 (ISSN-L)
- OpenAlex
- W2003316529
- Cited by
- 195
- References
- 2
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 3 · 2021: 13 · 2020: 7 · 2019: 9 · 2018: 13 · 2017: 8 · 2016: 13 · 2015: 11 · 2014: 12 · 2013: 18 · 2012: 12
- FWCI
- 9.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neurotoxicology and Teratology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- genistein, bisphenol A, endocrine active compounds, sexual differentiation, anteroventral periventricular nucleus, hypothalamus, tyrosine hydroxylase, estrogen receptor alpha, aromatization, estradiol, developmental neurotoxicity, sex differences, phytoestrogens, plastics
- MeSH terms
- Bisphenol A Compounds, Animals, Animals, Newborn, Benzhydryl Compounds, Cell Count, Cell Differentiation, Disease Models, Animal, Estradiol, Estrogens, Non-Steroidal, Female, Growth Inhibitors, Male, Nervous System Malformations, Phenols, Pregnancy, Preoptic Area, Sex Characteristics, Sex Differentiation, Tyrosine 3-Monooxygenase, Rats, Sprague-Dawley, Genistein, Estradiol Congeners, Estrogen Receptor alpha, Rats
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