Dietary-induced modulation of the hindgut microbiota is related to behavioral responses during stressful events in horses
Open accessAbstract
The bidirectional communication between the central and the enteric nervous system named the gut-brain axis has been widely recognized. The gut microbiota has been implicated in a variety of stress-related conditions including anxiety, depression and irritable bowel syndrome based on rodent studies or correlative analysis in human patients. The aim of the present study was to investigate to what extent changes in behavior during stressful events and in the microbial composition of the colonic ecosystem were associated in horses. The microbiota alterations were induced by a change from a high-fiber diet (100% hay, H diet) to a progressive low-fiber and high-starch diet (56% hay and 44% barley, HB diet) on six fistulated horses. Colonic total anaerobic, cellulolytic, amylolytic and lactate-utilizing bacteria were enumerated once on H diet and once on HB diet. Bacterial richness, diversity and structure at family and genus level were also determined. The behavior of horses was assessed through two standardized stressful tests: a novelty test and an umbrella test. The different alterations measured in the colonic microbiota demonstrated a lower fibrolytic capacity and a higher amylolytic capacity of the ecosystem when horses received HB compared to H diet. During the novelty test, the frequency of blowing was significantly higher in HB than in H diet and was positively correlated with the concentration of amylolytic bacteria and the Succinivibrionaceae relative abundance. During the umbrella test, behavioral variables were not significantly different between the diets but the colonic content pH was negatively correlated with the frequency of startle response. Behavioral responses of anxiety were related to hindgut microbiota indicators of a high-starch diet. Dietary-induced modulation of the gut microbiota composition may have changed the horses' behavioral reactions in stressful situations.
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In horses, a high-starch diet increased the abundance of amylolytic and total anaerobic bacteria and of Succinivibrionaceae compared with a high-fiber diet.
“↑ Concentration of amylolytic and total | anaerobic bacteria in HB diet; ↑ | abundance of Succinivibrionaceae in | HB diet”
From Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health (2021) · cites this paper as [343]
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Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health
Berding, K., Vlckova, K., Marx, W., Schellekens, H., Stanton, C., Clarke, G., Jacka, F., Dinan, T. G., & Cryan, J. F. (2021) · Advances in Nutrition · doi:10.1093/advances/nmaa181 · Cited by 451 · see citing works
- Authors
- Destrez, A., Grimm, P., & Julliand, V.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Procédés Alimentaires et Microbiologiques (FR)
- Corresponding author
- Destrez, Alexandra
- Venue
- Physiology & Behavior
- Publisher
- Elsevier BV
- Published
- 2019-04
- Volume
- 202
- Pages
- 94-100
- ISSN
- 0031-9384 (ISSN-L)
- OpenAlex
- W2912316851
- Cited by
- 42
- References
- 43
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 8 · 2023: 6 · 2022: 7 · 2021: 5 · 2020: 6 · 2019: 4
- FWCI
- 10.15
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Physiology & Behavior (acceptedVersion, other-oa)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by-nc)
- Topics
- Health Sciences › Veterinary › Equine › Veterinary Equine Medical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Keywords
- gut microbiota, horses, dietary fiber, high-starch diet, colonic microbiota, gut-brain axis, stress response, anxiety-like behavior, amylolytic bacteria, fibrolytic bacteria, Succinivibrionaceae, colonic pH, startle response
- MeSH terms
- Gastrointestinal Microbiome, Animals, Colon, Diet, Horses, Male, RNA, Ribosomal, 16S, Stress, Psychological
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