Collagen morphology influences macrophage shape and marker expression in?vitro
Open accessThe claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
M1 macrophages have an egg-shaped morphology, while M2 macrophages exhibit a more spindle-shaped morphology.
“It has been proved that M1 macrophages have an egg-shaped morphology, while M2 macrophages exhibited a more spindle-shaped morphology [31,38].”
From Substrate stiffness modulates bone marrow-derived macrophage polarization through NF-κB signaling pathway (2020) · cites this paper as [31]
In the Discussion passage headed “Combining these” (page not provided), the paper reports that collagen changed macrophage shape without changing macrophage subsets. It says a particular phenotype does not necessarily restrict macrophages to a certain shape, rather than establishing egg-shaped M1 and spindle-shaped M2 morphologies. (LLM, conf=0.86)
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measuredCD68 and CD86 are markers for M1 macrophages, and CD68 and CD206 are markers for M2 macrophages.
“Macrophage phenotypic markers, CD68 and CD86 for M1 and CD68 and CD206 for M2, were selected following the previous study [31,32].”
From Substrate stiffness modulates bone marrow-derived macrophage polarization through NF-κB signaling pathway (2020) · cites this paper as [31]
Section 2.5 defines M2 macrophages as CD206-high, supporting CD206 as an M2 marker. It defines M1 using MHCII-high and CD206-low, and does not identify CD68 or CD86 as markers for either phenotype. No page number is available in our copy. (LLM, conf=0.88)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
The M1 and M2 paradigm of macrophage activation: Time for reassessment
Martinez, F. O., & Gordon, S. (2014) · F1000Prime Reports · doi:10.12703/p6-13 · Cited by 4,689 · see citing works
Macrophages in Tissue Repair, Regeneration, and Fibrosis
Wynn, T. A., & Vannella, K. M. (2016) · Immunity · doi:10.1016/j.immuni.2016.02.015 · Cited by 4,481 · see citing works
Remodelling the extracellular matrix in development and disease
Bonnans, C., Chou, J., & Werb, Z. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3904 · Cited by 4,367 · see citing works
Stein, M., Keshav, S., Harris, N., & Gordon, S. (1992) · The Journal of experimental medicine · doi:10.1084/jem.176.1.287 · Cited by 1,960 · see citing works
Clinical course and prediction of survival in idiopathic pulmonary fibrosis
Ley, B., Collard, H. R., & King, T. E. (2011) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201006-0894ci · Cited by 1,765 · see citing works
Understanding the Mysterious M2 Macrophage through Activation Markers and Effector Mechanisms
Rőszer, T. (2015) · Mediators of Inflammation · doi:10.1155/2015/816460 · Cited by 1,762 · see citing works
Booth, A. J., Hadley, R., Cornett, A. M., Dreffs, A. A., Matthes, S. A., Tsui, J. L., Weiss, K., Horowitz, J. C., Fiore, V. F., Barker, T. H., et al. (2012) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201204-0754oc · Cited by 665 · see citing works
Blakney, A. K., Swartzlander, M. D., & Bryant, S. J. (2012) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.34104 · Cited by 470 · see citing works
Idiopathic pulmonary fibrosis: a disease with similarities and links to cancer biology
Vancheri, C., Failla, M., Crimi, N., & Raghu, G. (2010) · European Respiratory Journal · doi:10.1183/09031936.00077309 · Cited by 463 · see citing works
Differential expression of FIZZ1 and Ym1 in alternatively versus classically activated macrophages
Raes, G., BAETSELIER, P. D., Noël, W., Beschin, A., Brombacher, F., & Gh., G. H. (2002) · Journal of Leukocyte Biology · doi:10.1189/jlb.71.4.597 · Cited by 443 · see citing works
Molecular and cellular mechanisms of pulmonary fibrosis
Todd, N. W., Luzina, I. G., & Atamas, S. P. (2012) · Fibrogenesis & Tissue Repair · doi:10.1186/1755-1536-5-11 · Cited by 418 · see citing works
Kottmann, R. M., Kulkarni, A. A., Smolnycki, K. A., Lyda, E., Dahanayake, T., Salibi, R., Honnons, S., Jones, C., Isern, N. G., Hu, J. Z., et al. (2012) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201201-0084oc · Cited by 415 · see citing works
Wells, R. G. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2013.02.007 · Cited by 384 · see citing works
Hinz, B. (2009) · Current Rheumatology Reports · doi:10.1007/s11926-009-0017-1 · Cited by 373 · see citing works
Goethem, E. V., Poincloux, R., Gauffre, F., Maridonneau‐Parini, I., & Cabec, V. L. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0902223 · Cited by 364 · see citing works
The pathogenesis of pulmonary fibrosis: a moving target
Wuyts, W. A., Agostini, C., Antoniou, K. M., Bouros, D. E., Chambers, R. C., Cottin, V., Egan, J. J., Lambrecht, B. N., Lories, R. J. U., Parfrey, H., et al. (2012) · European Respiratory Journal · doi:10.1183/09031936.00073012 · Cited by 354 · see citing works
Madsen, D. H., Leonard, D., Masedunskas, A., Moyer, A. B., Jürgensen, H. J., Peters, D. E., Amornphimoltham, P., Selvaraj, A., Yamada, S. S., Brenner, D. A., et al. (2013) · The Journal of Cell Biology · doi:10.1083/jcb.201301081 · Cited by 350 · see citing works
Pechkovsky, D. V., Prasse, A., Kollert, F., Engel, K. M., Dentler, J., Luttmann, W., Friedrich, K., Müller–Quernheim, J., & Zissel, G. (2010) · Clinical Immunology · doi:10.1016/j.clim.2010.06.017 · Cited by 328 · see citing works
Cell Elasticity Determines Macrophage Function
Patel, N. R., Bole, M., Chen, C., Hardin, C. C., Kho, A. T., Mih, J., Deng, L., Butler, J., Tschumperlin, D., Fredberg, J. J., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0041024 · Cited by 275 · see citing works
Always cleave up your mess: targeting collagen degradation to treat tissue fibrosis
McKleroy, W., Lee, T., & Atabai, K. (2013) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00418.2012 · Cited by 263 · see citing works
Ploeger, D. T. A., Hosper, N. A., Schipper, M., Koerts, J. A., de Rond, S., & Bank, R. A. (2013) · Cell Communication and Signaling · doi:10.1186/1478-811x-11-29 · Cited by 260 · see citing works
Directed migration of mesenchymal cells: where signaling and the cytoskeleton meet
Bear, J. E., & Haugh, J. M. (2014) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2014.06.005 · Cited by 189 · see citing works
Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
Vogel, D. Y. S., Heijnen, P. D. A. M., Breur, M., de Vries, H. E., Tool, A. T. J., Amor, S., & Dijkstra, C. D. (2014) · Journal of Neuroinflammation · doi:10.1186/1742-2094-11-23 · Cited by 180 · see citing works
Macrophage Heterogeneity in Respiratory Diseases
Boorsma, C. E., Draijer, C., & Melgert, B. N. (2013) · Mediators of Inflammation · doi:10.1155/2013/769214 · Cited by 146 · see citing works
Self-citationSensitivity of alveolar macrophages to substrate mechanical and adhesive properties
Féréol, S., Fodil, R., Labat, B., Galiacy, S., Laurent, V. M., Louis, B., Isabey, D., & Planus, E. (2006) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20130 · Cited by 139 · see citing works
Idiopathic pulmonary fibrosis: An update
Spagnolo, P., Sverzellati, N., Rossi, G., Cavazza, A., Τzouvelekis, Α., Crestani, B., & Vancheri, C. (2015) · Annals of Medicine · doi:10.3109/07853890.2014.982165 · Cited by 121 · see citing works
Fejér, G., Wegner, M., Győry, I., Cohen, I., Engelhard, P., Voronov, E., Manke, T., Ruzsics, Z., Dölken, L., da Costa, O. P., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1302877110 · Cited by 112 · see citing works
Bioelectric modulation of macrophage polarization
Li, C., Levin, M. G., & Kaplan, D. L. (2016) · Scientific Reports · doi:10.1038/srep21044 · Cited by 108 · see citing works
Collagen binding by the mannose receptor mediated through the fibronectin type II domain
Napper, C. E., Drickamer, K., & Taylor, M. E. (2006) · Biochemical Journal · doi:10.1042/bj20052027 · Cited by 98 · see citing works
Cai, Y., Kumar, R. K., Zhou, J., Foster, P. S., & Webb, D. C. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0803874 · Cited by 92 · see citing works
Chung, W., Chang, A. C., Kato, I., & Chang, N.-C. A. (2002) · Journal of Leukocyte Biology · doi:10.1189/jlb.72.1.72 · Cited by 88 · see citing works
Gowen, B. B., Borg, T. K., Ghaffar, A., & Mayer, E. P. (2000) · Matrix Biology · doi:10.1016/s0945-053x(99)00052-9 · Cited by 68 · see citing works
Kottmann, R. M., Sharp, J., Owens, K. M., Salzman, P., Xiao, G., Phipps, R. P., Sime, P. J., Brown, E. B., & Perry, S. W. (2015) · Respiratory Research · doi:10.1186/s12931-015-0220-8 · Cited by 57 · see citing works
Quantification of collagen I in airway tissues using second harmonic generation
Tjin, G., Xu, P., Kable, S. H., Kable, E., & Burgess, J. K. (2014) · Journal of Biomedical Optics · doi:10.1117/1.jbo.19.3.036005 · Cited by 55 · see citing works
Mannose receptor regulation of macrophage cell migration
Sturge, J., Todd, S. K., Kogianni, G., McCarthy, A., & Isacke, C. M. (2007) · Journal of Leukocyte Biology · doi:10.1189/jlb.0107053 · Cited by 48 · see citing works
Zhao, J., Lv, Z., Wang, F., Wei, J., Zhang, Q., Li, S., Yang, F., Zeng, X., Wu, X., & Wu, Z. (2013) · Parasitology Research · doi:10.1007/s00436-013-3436-x · Cited by 34 · see citing works
Pirfenidone and Nintedanib for Treatment of Idiopathic Pulmonary Fibrosis
Sathiyamoorthy, G., Sehgal, S., & Ashton, R. W. (2017) · Southern Medical Journal · doi:10.14423/smj.0000000000000655 · Cited by 30 · see citing works
Emerging therapeutic interventions for idiopathic pulmonary fibrosis
Chakraborty, S., Chopra, P., Ambi, S. V., Dastidar, S. G., & Ray, A. (2014) · Expert Opinion on Investigational Drugs · doi:10.1517/13543784.2014.913569 · Cited by 25 · see citing works
Substrate Stiffness Regulates the Behavior of Human Monocyte-Derived Macrophages
Hayenga, H. N., Adlerz, K. M., & Aranda‐Espinoza, H. (2015) · Biophysical Journal · doi:10.1016/j.bpj.2014.11.1667 · Cited by 5 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [21]21. Blakney AK, Swartzlander MD, Bryant SJ. The effects of substrate stiffness on the in vitro activation of macrophages and in vivo host response to poly(- ethylene glycol)-based hydrogels. J Biomed Mater Res A. 2012;100:1375e1386.
Cited by
Sorted by citations
Chen, M., Zhang, Y., Zhou, P., Liu, X., Zhao, H., Zhou, X., Gu, Q., Li, B., Zhu, X., & Shi, Q. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.05.004 · Cited by 229 · see citing works
Inhibiting Bacterial Adhesion by Mechanically Modulated Microgel Coatings
Keskin, D., Mergel, O., van der Mei, H. C., Busscher, H. J., & van Rijn, P. (2018) · Biomacromolecules · doi:10.1021/acs.biomac.8b01378 · Cited by 84 · see citing works
Collagen Fibril Density Modulates Macrophage Activation and Cellular Functions during Tissue Repair
Sapudom, J., Mohamed, W. K. E. A., Garcia‐Sabaté, A., Alatoom, A., Karaman, S., Mahtani, N., & Teo, J. C. M. (2020) · Bioengineering · doi:10.3390/bioengineering7020033 · Cited by 72 · see citing works
Macrophage–stroma interactions in fibrosis: biochemical, biophysical, and cellular perspectives
Vasse, G. F., Nizamoglu, M., Heijink, I. H., Schlepütz, M., van Rijn, P., Thomas, M. J., Burgess, J. K., & Melgert, B. N. (2021) · The Journal of Pathology · doi:10.1002/path.5632 · Cited by 66 · see citing works
Self-citationChronic lung diseases: entangled in extracellular matrix
Burgess, J. K., & Harmsen, M. C. (2022) · European Respiratory Review · doi:10.1183/16000617.0202-2021 · Cited by 65 · see citing works
Immunomodulatory Scaffolds Derived from Lymph Node Extracellular Matrices
Choi, Y. S., Jeong, E., Lee, J. S., Kim, S. K., Jo, S.-H., Kim, Y., Sung, H., Cho, S., & Jin, Y. (2021) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.1c02542 · Cited by 43 · see citing works
Integration of immune cells in organs-on-chips: a tutorial
van Os, L., Engelhardt, B., & Guenat, O. T. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1191104 · Cited by 39 · see citing works
Joglekar, M. M., Nizamoglu, M., Fan, Y., Nemani, S. S. P., Weckmann, M., Pouwels, S. D., Heijink, I. H., Melgert, B. N., Pillay, J., & Burgess, J. K. (2022) · Frontiers in Pharmacology · doi:10.3389/fphar.2022.995051 · Cited by 24 · see citing works
Self-citationExtracellular Matrix as a Driver of Chronic Lung Diseases
Burgess, J. K., Weiss, D. J., Westergren‐Thorsson, G., Wigén, J., Dean, C. H., Mumby, S. E., Bush, A., & Adcock, I. M. (2024) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2023-0176ps · Cited by 24 · see citing works
Polarization of M2 Tumor-Associated Macrophages (TAMs) in Cancer Immunotherapy
Bui, I., & Bonavida, B. (2024) · Critical Reviews™ in Oncogenesis · doi:10.1615/critrevoncog.2024053830 · Cited by 18 · see citing works
Luan, Y., van der Mei, H. C., Dijk, M., Geertsema-Doornbusch, G. I., Atema‐Smit, J., Ren, Y., Chen, H., & Busscher, H. J. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b01518 · Cited by 16 · see citing works
Extracellular Matrix in Cardiac Tissue Mechanics and Physiology: Role of Collagen Accumulation
LeBar, K., & Wang, Z. (2021) · Biochemistry · doi:10.5772/intechopen.96585 · Cited by 15 · see citing works
Deville, S., Romeu, H. G., Oeyen, E., Mertens, I., Nelissen, I., & Salvati, A. L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms24010260 · Cited by 13 · see citing works
Extracellular matrix architecture promotes immunosuppressive microenvironments in pancreatic cancer
Callaway, M. K., Noonan, B. J., Schwertfeger, K. L., & Provenzano, P. P. (2025) · Matrix Biology · doi:10.1016/j.matbio.2025.09.004 · Cited by 11 · see citing works
Maes, L., Szabó, A., Haevermaete, J. V., Geurs, I., Dewettinck, K., Vandenbroucke, R. E., Vlierberghe, S. V., & Laukens, D. (2025) · Biomaterials Advances · doi:10.1016/j.bioadv.2025.214232 · Cited by 8 · see citing works
Vasse, G. F., Russo, S. L. M. L., Barcaru, A., Oun, A., Dolga, A. M., van Rijn, P., Kwiatkowski, M., Govorukhina, N. I., Bischoff, R., & Melgert, B. N. (2023) · Scientific Reports · doi:10.1038/s41598-023-32715-0 · Cited by 9 · see citing works
Self-citationThe Crucial Nexus: Unveiling the Role of Collagen in Cancer Progression
Reeva, H., Mahesh, G., Manjunath, U., Selvamurugan, N., & Mohankumar, D. (2025) · Seminars in Oncology · doi:10.1016/j.seminoncol.2025.152346 · Cited by 7 · see citing works
Yuan, Y., Ren, Y., van Dijk, M. C., Geertsema‐Doornbusch, G. I., Atema‐Smit, J., Busscher, H., & van der Mei, H. (2021) · European Cells and Materials · doi:10.22203/ecm.v041a27 · Cited by 6 · see citing works
Qi, L., Dang, G., Ling, X., Miao, Y., Bo, Y., Zhai, Y., Chen, X., Zhai, Q., Zheng, L., Zhang, Y., et al. (2026) · Cancer Research · doi:10.1158/0008-5472.can-25-2871 · Cited by 8 · see citing works
Xiao, M., Sun, L., Fu, H., Yang, W., Yao, J., Shao, Z., Ling, S., Zhao, B., & Chen, X. (2025) · Journal of Materials Chemistry B · doi:10.1039/d5tb00508f · Cited by 4 · see citing works
Chen, K., Lin, M., Zhou, D., Xu, K., Mei, J., Chang, C., Zhu, M., & Zhou, C. (2026) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2025.124884 · Cited by 3 · see citing works
Biomaterials modulate macrophage polarization and inflammatory responses
Zheng, W., Huang, H., Guo, F., Yuan, C., & Zhou, Q. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-822368-0.00006-2 · Cited by 3 · see citing works
Self-citationGupta, T., Sahu, R. P., Dabaghi, M., Zhong, L. S., Shargall, Y., Hirota, J. A., Richards, C. D., & Puri, I. K. (2023) · ACS Omega · doi:10.1021/acsomega.3c02052 · Cited by 1 · see citing works
Yang, H., Kim, Y., & Wu, L. (2021) · Royal Society of Chemistry eBooks · doi:10.1039/9781839163975-00383
Macrophage metabolic reprogramming in chronic diseases
Russo, S. L. M. L. (2024) · doi:10.33612/diss.922211372
- Authors
- Vasse, G. F., Kühn, P. T., Zhou, Q., Bhusari, S. A., Reker-Smit, C., Melgert, B. N., & van Rijn, P.
- Affiliations
- University Medical Center Groningen (NL)
- Corresponding author
- Melgert, Barbro N.
- Venue
- Journal of Immunology and Regenerative Medicine
- Publisher
- Elsevier BV
- Published
- 2018-03
- Volume
- 1
- Pages
- 13-20
- ISSN
- 2468-4988 (ISSN-L)
- OpenAlex
- W2802861923
- Cited by
- 25
- References
- 39
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 5 · 2020: 3 · 2018: 1
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Immunology and Regenerative Medicine (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion, other-oa)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion, other-oa)
- Funders
- University Medical Center Groningen
- China Sponsorship Council (201406630003)
- UMCG Microscopy and Imaging Center (UMIC) (40-00506-98-9021)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- macrophage polarization, type I collagen, collagen morphology, extracellular matrix stiffness, pulmonary fibrosis, CD206, Ym1, filopodia, actin cytoskeleton, M2 macrophages, idiopathic pulmonary fibrosis, extracellular matrix mechanics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
