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Cited by 32Bartonella species infections researchBurkholderia infections and melioidosisBacillus and Francisella bacterial research

Molecular detection of Bartonella henselae and Bartonella clarridgeiae in clinical samples of pet cats from Southern Italy

Pennisi, M.G., La Camera, E., Giacobbe, L., Orlandella, B.M., Lentini, V., Zummo, S., Fera, M.T.·2010·Research in Veterinary Science
DOI 10.1016/j.rvsc.2009.11.005
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Abstract

Bartonella henselae is considered an emerging pathogen of veterinary and medical interest that can be occasionally transmitted to humans. Cats are considered to be the only reservoir host for B. henselae. In this study, we used a nested-PCR assay to investigate the prevalence of B.henselae and Bartonella clarridgeiae DNA in peripheral blood samples, fine needle lymph node aspirate specimens and oral swabs from 85 cats in order to develop an easy diagnostic strategy for the selection of infection-free cats that are being considered as pets, especially for immunocompromised patients. Overall, molecular analysis showed that 71 cats (83.5%) tested PCR positive for the presence of B. henselae DNA. PCR amplification of DNA B. henselae produced positive products from lymph node aspirate specimens (62/85; 72.9%) similar to those obtained from blood samples (60/85; 70.6%) and higher than those from oral swabs (51/85; 60%) of cats. No PCR product was obtained for B. clarridgeiae. The simultaneous analysis of three different clinical samples in our study increased the diagnostic possibilities for B. henselae infection in the examined cats from 60-72.9% to 83.5%. Lymph node aspirates were found to be the most effective clinical samples for the detection of B. henselae and blood samples were the next best. Oral swab samples were used in this study with good results when considered in combination with blood and/or lymph node aspiration. The use of nested-PCR assay on these three clinical samples may enhance the diagnostic sensitivity for bartonellosis in cats irrespective of the clinical status of animals.

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  • In Italy, Bartonella spp. is endemic especially in the South, where human seropositivity to cat scratch disease has been unveiled.

    “In Italy, Bartonella spp. is endemic especially in the South [165,166], where human seropositivity to cat scratch disease has been unveiled [167].”

    From Fleas infesting pets in the era of emerging extra-intestinal nematodes (2013) · cites this paper as [165]

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Authors
Pennisi, M. G., La Camera, E., Giacobbe, L., Orlandella, B. M., Lentini, V., Zummo, S., & Fera, M. T.
Affiliations
University of Messina (IT)
Corresponding author
Fera, M.T.
ORCID
Venue
Research in Veterinary Science
Publisher
Elsevier BV
Published
2010-06
Volume
88
Issue
3
Pages
379-384
ISSN
0034-5288 (ISSN-L)
DOI
10.1016/j.rvsc.2009.11.005
OpenAlex
W2019903441
Cited by
32
References
38
Citations by year
2026: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 2
FWCI
1.20
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Research in Veterinary Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Bartonella species infections research
  • Health Sciences › Medicine › Epidemiology › Burkholderia infections and melioidosis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacillus and Francisella bacterial research
Keywords
Bartonella henselae, Bartonella clarridgeiae, feline bartonellosis, cats, zoonotic transmission, lymph node aspirates, peripheral blood, oral swabs, diagnostic sensitivity, nested PCR, immunocompromised patients
MeSH terms
Animals, Animals, Domestic, Bartonella, Bartonella Infections, Cats, DNA, Bacterial, Female, Siphonaptera, Gene Amplification, Humans, Italy, Lymph Nodes, Male, Mouth, Tick Infestations, Polymerase Chain Reaction, Bartonella henselae

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