The DOI matches its Crossref record; 84 of the 84 references it cites were found in a registry or an index.
Impacts of polystyrene microplastic on the gut barrier, microbiota and metabolism of mice
Abstract
Microplastics (MPs), which are new environmental pollutants with a diameter of <5 mm, have received wide attention in recent years. However, there are still very limited data regarding the risks of MPs to animals, especially higher mammals. In this study, we exposed male mice to 5 μm pristine and fluorescent polystyrene MP for six weeks. The results showed that the polystyrene MP was observed in the guts of mice and could reduce the intestinal mucus secretion and cause damage the intestinal barrier function. In addition, high-throughput sequencing of the V3-V4 region of the 16S rRNA gene was used to explore the change of the gut microbiota composition in the cecal content. At the phylum level, the content of Actinobacteria decreased significantly in the polystyrene MP-treated group. The PD whole-tree indexes of the alpha diversity and principal component analysis (PCA) of the beta diversity indicated that the diversity of gut microbiota was altered after polystyrene MP exposure. At the genus level, a total of 15 types of bacteria changed significantly after exposure to polystyrene MP. Furthermore, the predicted KEGG (Kyoto Encyclopedia of Genes and Genomes) metabolic pathway differences indicated that the main metabolic pathways of the functional genes in the microbial community were significantly influenced by the polystyrene MP. In addition, indexes of amino acid metabolism and bile acid metabolism in the serum were analyzed after polystyrene MP exposure. These results indicated that polystyrene MP caused metabolic disorders. In conclusion, the polystyrene MP induced gut microbiota dysbiosis, intestinal barrier dysfunction and metabolic disorders in mice. This study provided more data on the toxicity of MPs in a terrestrial organism to aid in the assessment of the health risks of polystyrene MP to animals.
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In the citing paper
“and metabolic disorders (5-μm polystyrene microplastics at concentrations of 1.456×106 particles/L and 1.456×107 particles/L).31”
From Microplastics and Anthropogenic Particles in Recreationally Caught Freshwater Fish from an Urbanized Region of the North American Great Lakes (2024) · cites this paper as [31]
In the citing paper
“Although some in vitro and in vivo studies examining impacts of microplastics on mammals show effects, such as intestinal barrier disfunction and alteration of biomarkers,30,31”
From Microplastics and Anthropogenic Particles in Recreationally Caught Freshwater Fish from an Urbanized Region of the North American Great Lakes (2024) · cites this paper as [31]
In the citing paper
“However, it is unclear if toxicological effects from either of these mechanisms are occurring in humans, and at what thresholds effects occur.”
From Microplastics and Anthropogenic Particles in Recreationally Caught Freshwater Fish from an Urbanized Region of the North American Great Lakes (2024) · cites this paper as [31]
In the citing paper
“Smaller nanoparticles exhibit a higher surface area-to-volume ratio, enhancing their chemical reactivity, biological interactions, cellular uptake, and tissue penetration (Jin et al., 2019; Shin et al., 2023; Teng et al., 2022).”
From Deciphering the toxic effects of polystyrene nanoparticles on erythropoiesis at single-cell resolution (2025) · cites this paper as Jin et al., 2019
Citations
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Plastic waste inputs from land into the ocean
Jambeck, J. R., Geyer, R., Wilcox, C., Siegler, T. R., Perryman, M., Andrady, A., Narayan, R., & Law, K. L. (2015) · Science · doi:10.1126/science.1260352 · Cited by 13,163
Structure, function and diversity of the healthy human microbiome
The Human Microbiome Project Consortium (2012) · Nature · doi:10.1038/nature11234 · Cited by 11,726
Lost at sea: where is all the plastic?
Thompson, R. C., Olsen, Y., Mitchell, R. P., Davis, A., Rowland, S. J., John, A. W. G., McGonigle, D., & Russell, A. E. (2004) · Science · doi:10.1126/science.1094559 · Cited by 7,765
Obesity alters gut microbial ecology
Ley, R. E., Bäckhed, F., Turnbaugh, P., Lozupone, C. A., Knight, R. D., & Gordon, J. I. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504978102 · Cited by 6,176
Accumulation and fragmentation of plastic debris in global environments
Barnes, D. K. A., Galgani, F., Thompson, R. C., & Barlaz, M. (2009) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2008.0205 · Cited by 6,126
Metagenomic Analysis of the Human Distal Gut Microbiome
Gill, S. R., Pop, M., DeBoy, R. T., Eckburg, P. B., Turnbaugh, P. J., Samuel, B. S., Gordon, J. I., Relman, D. A., Fraser-Liggett, C. M., & Nelson, K. E. (2006) · Science · doi:10.1126/science.1124234 · Cited by 4,699
Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour
Cryan, J. F., & Dinan, T. G. (2012) · Nature Reviews Neuroscience · doi:10.1038/nrn3346 · Cited by 4,572
The Physical Impacts of Microplastics on Marine Organisms: A Review
Wright, S. L., Thompson, R. C., & Galloway, T. S. (2013) · Environmental Pollution · doi:10.1016/j.envpol.2013.02.031 · Cited by 4,524
Horton, A. A., Walton, A., Spurgeon, D. J., Lahive, E., & Svendsen, C. (2017) · Science of The Total Environment · doi:10.1016/j.scitotenv.2017.01.190 · Cited by 3,756
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Risk assessment of microplastic particles
Koelmans, A. A., Redondo‐Hasselerharm, P. E., Nor, N. H. M., de Ruijter, V. N., Mintenig, S. M., & Kooi, M. (2022) · Nature Reviews Materials · doi:10.1038/s41578-021-00411-y · Cited by 1,204
Impact of Microplastics and Nanoplastics on Human Health
Yee, M. S.-L., Hii, L., Looi, C.-K., Lim, W., Wong, S. F., Kok, Y.-Y., Tan, B.-K., Wong, C.-Y., & Leong, C. (2021) · Nanomaterials · doi:10.3390/nano11020496 · Cited by 1,119
Ecotoxicity and genotoxicity of polystyrene microplastics on higher plant Vicia faba
Jiang, X., Chen, H., Liao, Y., Ye, Z., Li, M., & Klobučar, G. I. V. (2019) · Environmental Pollution · doi:10.1016/j.envpol.2019.04.055 · Cited by 1,008
Akdiş, C. A. (2021) · Nature reviews. Immunology · doi:10.1038/s41577-021-00538-7 · Cited by 1,066
Toxicity of Microplastics and Nanoplastics in Mammalian Systems
Yong, C., Valiyaveettil, S., & Tang, B. L. (2020) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph17051509 · Cited by 925
“Pathological changes to the gut include a reduction in mucus secretion [90], gut barrier dysfunction [104,106], intestinal inflammation [107] and gut microbiota dysbiosis [90,104,106,107].”
Background
Metadata
- Authors
- Jin, Y., Lu, L., Tu, W., Luo, T., & Fu, Z.
- Affiliations
- Zhejiang University of Technology (CN)
- Jiangxi Academy of Sciences (CN)
- Corresponding author
- Fu, Zhengwei
- Venue
- Science of The Total Environment
- Publisher
- Elsevier BV
- Published
- 2019-02
- Volume
- 649
- Pages
- 308-317
- ISSN
- 0048-9697 (ISSN-L)
- OpenAlex
- W2889479406
- Cited by
- 1,100
- References
- 84
- Citations by year
- 2026: 152 · 2025: 189 · 2024: 193 · 2023: 161 · 2022: 181 · 2021: 102 · 2020: 73 · 2019: 39
- FWCI
- 20.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Science of The Total Environment (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Zhejiang Provincial Natural Science Foundation of China (LR16B070002, 21777146)
- National Natural Science Foundation of China
- Topics
- Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
- Keywords
- polystyrene microplastics, gut dysbiosis, intestinal barrier dysfunction, microbial metabolic pathways, amino acid metabolism, bile acid metabolism, cecal microbiota, actinobacteria
- MeSH terms
- Gastrointestinal Microbiome, Animals, Environmental Pollutants, Male, Metabolic Diseases, Mice, Inbred ICR, Particle Size, Polystyrenes, Gastrointestinal Tract, Mice, Particulate Matter, Dysbiosis
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