Comprehensive understanding the impacts of dietary exposure to polyethylene microplastics on genetically improved farmed tilapia (Oreochromis niloticus): tracking from growth, microbiota, metabolism to gene expressions
Abstract
Microplastics (MPs) pollution has been recognized as a threat to sustainable fisheries due to the risks of MPs contamination in the process of feed production and susceptibility of fish to ingest MPs from the aquatic environment. In this study, we applied comprehensive approaches to investigate the impacts of polyethylene microplastics (PE-MPs) on juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus) through 9-week dietary exposure based on growth performance, gut microbiota, liver metabolism, and gene expressions in brain and liver tissues. Dietary exposure to two kinds of PE-MPs with different median size (27 μm and 63 μm, respectively) concentration-dependently decreased weight gain (WG), while increased feed conversion ratio (FCR) and hepatosomatic index (HSI) of the tilapia. Dietary administration of PE-MPs also significantly reduced the activities of intestinal protease and amylase. PE-MPs particles of the larger size groups (63 μm) were mainly detected in feces, but those of the smaller ones (27 μm) tended to be accumulated in intestine. PE-MPs ingestion resulted in the alteration of gut microbiota composition, with Fusobacteria, Verrucomicrobia and Firmicutes as the overrepresented bacterial taxa. Metabolomic assays of liver samples in fish fed the diets containing 8 % of PE-MPs revealed the particle size-specific variations in composition of differential metabolites and metabolism pathways such as amino acid and glycerophospholipid metabolism. Gene expressions of brain and liver samples were analyzed by RNA-seq. Photoperiodism and circadian rhythm were the representative biological processes enriched for the differentially expressed genes (DEGs) identified from the brain. Citrate cycle (TCA cycle) was the most enriched pathway revealed by a joint transcriptomic and metabolic pathway analysis for the liver, followed by propanoate and pyruvate metabolism. Furthermore, an integration analysis of the gut microbiome and liver transcriptome data identified significant associations between several pathogenic bacteria taxa and immune pathways. Our findings demonstrated that the sizes and concentrations of PE-MPs are critically related to their toxic impacts on microbiota community, metabolism, gene expressions and thus fish growth.
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Laboratory studies have demonstrated microplastics can cause a variety of detrimental effects on fish, including negative effects on growth, changes in reproduction, and reduced survival.
“Laboratory studies have demonstrated microplastics can cause a variety of detrimental effects on fish, including negative effects on growth,74 changes in reproduction, and reduced survival.73”
From Microplastics and Anthropogenic Particles in Recreationally Caught Freshwater Fish from an Urbanized Region of the North American Great Lakes (2024) · cites this paper as [74]
Cites
Cytoscape: A software Environment for integrated models of biomolecular interaction networks
Shannon, P., Markiel, A., Ozier, O., Baliga, N. S., Wang, J. T., Ramage, D., Amin, N., Schwikowski, B., & Ideker, T. (2003) · Genome Research · doi:10.1101/gr.1239303 · Cited by 51,888 · see citing works
DADA2: High–resolution sample inference from Illumina amplicon data
Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A., & Holmes, S. P. (2016) · Nature Methods · doi:10.1038/nmeth.3869 · Cited by 35,698 · see citing works
Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2
Bolyen, E., Rideout, J. R., Dillon, M. R., Bokulich, N. A., Abnet, C. C., Al-Ghalith, G. A., Alexander, H., Alm, E. J., Arumugam, M., Asnicar, F., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0209-9 · Cited by 23,742 · see citing works
Production, use, and fate of all plastics ever made
Geyer, R., Jambeck, J. R., & Law, K. L. (2017) · Science Advances · doi:10.1126/sciadv.1700782 · Cited by 18,606 · see citing works
Plastic waste inputs from land into the ocean
Jambeck, J. R., Geyer, R., Wilcox, C., Siegler, T. R., Perryman, M., Andrady, A., Narayan, R., & Law, K. L. (2015) · Science · doi:10.1126/science.1260352 · Cited by 13,163 · see citing works
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
DeSantis, T. Z., Hugenholtz, P., Larsen, N., Rojas, M., Brodie, E. L., Keller, K., Huber, T., Dalevi, D., Hu, P., & Andersen, G. L. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.03006-05 · Cited by 10,891 · see citing works
Andersen, C. L., Jensen, J. L., & Ørntoft, T. F. (2004) · Cancer Research · doi:10.1158/0008-5472.can-04-0496 · Cited by 7,127 · see citing works
Bindea, G., Mlecnik, B., Hackl, H., Charoentong, P., Tosolini, M., Kirilovsky, A., Fridman, W.-H., Pagès, F., Trajanoski, Z., & Galon, J. (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp101 · Cited by 6,280 · see citing works
Accumulation and fragmentation of plastic debris in global environments
Barnes, D. K. A., Galgani, F., Thompson, R. C., & Barlaz, M. (2009) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2008.0205 · Cited by 6,126 · see citing works
The Physical Impacts of Microplastics on Marine Organisms: A Review
Wright, S. L., Thompson, R. C., & Galloway, T. S. (2013) · Environmental Pollution · doi:10.1016/j.envpol.2013.02.031 · Cited by 4,524 · see citing works
A cross-platform toolkit for mass spectrometry and proteomics
Chambers, M., MacLean, B., Burke, R., Amodei, D., Ruderman, D., Neumann, S., Gatto, L., Fischer, B., Pratt, B. S., Egertson, J. D., et al. (2012) · Nature Biotechnology · doi:10.1038/nbt.2377 · Cited by 4,042 · see citing works
MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights
Pang, Z., Chong, J., Zhou, G., Morais, D. A. D. L., Chang, L., Barrette, M., Gauthier, C., Jacques, P., Li, S., & Xia, J. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab382 · Cited by 3,972 · see citing works
Metsalu, T., & Vilo, J. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv468 · Cited by 3,729 · see citing works
Plastic and Human Health: A Micro Issue?
Wright, S. L., & Kelly, F. J. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.7b00423 · Cited by 3,413 · see citing works
Plastics Recycling: Challenges and Opportunities
Hopewell, J., Dvorak, R., & Kosior, E. (2009) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2008.0311 · Cited by 3,201 · see citing works
Thévenot, E., Roux, A., Xu, Y., Ezan, É., & Junot, C. (2015) · Journal of Proteome Research · doi:10.1021/acs.jproteome.5b00354 · Cited by 1,757 · see citing works
Wear and Tear of Tyres: A Stealthy Source of Microplastics in the Environment
Kole, P. J., Löhr, A., Belleghem, F. V., & Ragas, A. M. J. (2017) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph14101265 · Cited by 1,622 · see citing works
Microplastics in freshwater ecosystems: what we know and what we need to know
Wagner, M., Scherer, C., Alvarez-Muñoz, D., Brennholt, N., Bourrain, X., Buchinger, S., Fries, E., Grosbois, C., Klasmeier, J., Marti, T., et al. (2014) · Environmental Sciences Europe · doi:10.1186/s12302-014-0012-7 · Cited by 1,543 · see citing works
Circadian rhythms from flies to human
Panda, S., Hogenesch, J. B., & Kay, S. A. (2002) · Nature · doi:10.1038/417329a · Cited by 1,044 · see citing works
Qiao, R., Sheng, C., Lu, Y., Zhang, Y., Ren, H., & Lemos, B. (2019) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2019.01.245 · Cited by 971 · see citing works
Rawls, J. F., Mahowald, M. A., Ley, R. E., & Gordon, J. I. (2006) · Cell · doi:10.1016/j.cell.2006.08.043 · Cited by 962 · see citing works
Microplastics in surface waters and sediments of the Three Gorges Reservoir, China
Di, M., & Wang, J. (2017) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2017.10.150 · Cited by 945 · see citing works
Polystyrene microplastics induce microbiota dysbiosis and inflammation in the gut of adult zebrafish
Jin, Y., Xia, J., Pan, Z., Yang, J., Wang, W., & Fu, Z. (2018) · Environmental Pollution · doi:10.1016/j.envpol.2017.12.088 · Cited by 873 · see citing works
Ding, J., Zhang, S., Razanajatovo, R. M., Zou, H., & Zhu, W. (2018) · Environmental Pollution · doi:10.1016/j.envpol.2018.03.001 · Cited by 766 · see citing works
A high-performance protocol for extraction of microplastics in fish
Karami, A., Golieskardi, A., Choo, C. K., Romano, N., Ho, Y. B., & Salamatinia, B. (2016) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2016.10.213 · Cited by 764 · see citing works
Rochman, C. M., Kurobe, T., Flores, I., & Teh, S. J. (2014) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2014.06.051 · Cited by 762 · see citing works
Burns, E. E., & Boxall, A. B. A. (2018) · Environmental Toxicology and Chemistry · doi:10.1002/etc.4268 · Cited by 713 · see citing works
Current state and future prospects of plastic waste as source of fuel: A review
Wong, S. L., Ngadi, N., Abdullah, T. A. T., & Inuwa, I. M. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.04.063 · Cited by 701 · see citing works
Kim, J., Yu, Y.-B., & Choi, J.-H. (2021) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2021.125423 · Cited by 512 · see citing works
Relevance of nano- and microplastics for freshwater ecosystems: A critical review
Triebskorn, R., Braunbeck, T. A., Grummt, T., Hanslik, L., Huppertsberg, S., Jekel, M. R., Knepper, T. P., Krais, S., Müller, Y. K., Pittroff, M., et al. (2018) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2018.11.023 · Cited by 511 · see citing works
Wan, Z., Wang, C., Zhou, J., Shen, M., Wang, X., Fu, Z., & Jin, Y. (2018) · Chemosphere · doi:10.1016/j.chemosphere.2018.11.070 · Cited by 503 · see citing works
Cheung, P. K., & Fok, L. (2017) · Water Research · doi:10.1016/j.watres.2017.05.053 · Cited by 490 · see citing works
Microplastics and the gut microbiome: How chronically exposed species may suffer from gut dysbiosis
Fackelmann, G., & Sommer, S. (2019) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2019.04.030 · Cited by 385 · see citing works
Effects of Virgin Microplastics on Goldfish (Carassius auratus)
Jabeen, K., Li, B., Chen, Q., Su, L., Wu, C., Hollert, H., & Shi, H. (2018) · Chemosphere · doi:10.1016/j.chemosphere.2018.09.031 · Cited by 369 · see citing works
Wang, Y., Hamid, N., Deng, S., Jia, P., & Pei, D. (2020) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.122795 · Cited by 322 · see citing works
Microplastics in fish and fishmeal: an emerging environmental challenge?
Thiele, C. J., Hudson, M. D., Russell, A. E., Saluveer, M., & Sidaoui-Haddad, G. (2021) · Scientific Reports · doi:10.1038/s41598-021-81499-8 · Cited by 304 · see citing works
Hamed, M., Soliman, H. A. M., Osman, A. G. M., & Sayed, A. E. H. (2019) · Chemosphere · doi:10.1016/j.chemosphere.2019.04.153 · Cited by 281 · see citing works
Huang, J.-N., Wen, B., Zhu, J.-G., Zhang, Y.-S., Gao, J., & Chen, Z. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.138929 · Cited by 277 · see citing works
Ding, J., Huang, Y., Liu, S., Zhang, S., Zou, H., Wang, Z., Zhu, W., & Geng, J. (2020) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.122693 · Cited by 270 · see citing works
Benton, H. P., Wong, D. M., Trauger, S. A., & Siuzdak, G. E. (2008) · Analytical Chemistry · doi:10.1021/ac800795f · Cited by 266 · see citing works
Aquaculture role in global food security with nutritional value: a review
Pradeepkiran, J. A. (2019) · Translational Animal Science · doi:10.1093/tas/txz012 · Cited by 254 · see citing works
Zhao, Y., Qiao, R., Zhang, S., & Wang, G. (2020) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.123663 · Cited by 241 · see citing works
Zhang, X., Wen, K., Ding, D., Liu, J., Lei, Z., Chen, X., Ye, G., Zhang, J., Shen, H., Yan, C., et al. (2021) · Environment International · doi:10.1016/j.envint.2021.106452 · Cited by 221 · see citing works
Self-citationHamed, M., Soliman, H. A. M., Osman, A. G. M., & Sayed, A. E. H. (2020) · Environmental Science and Pollution Research · doi:10.1007/s11356-020-07898-y · Cited by 213 · see citing works
Naidoo, T., & Glassom, D. (2019) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2019.02.037 · Cited by 208 · see citing works
TGF-β Signaling: From Tissue Fibrosis to Tumor Microenvironment
Chung, J. Y., Chan, M. K., Li, J. S.-F., Chan, A. S. W., Tang, P. C., Leung, K., To, K. F., Lan, H. Y., & Tang, P. M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22147575 · Cited by 204 · see citing works
Occurrence of microplastics in fishes from two landing sites in Tuticorin, South east coast of India
Kumar, V. E., Ravikumar, G., & Jeyasanta, K. I. (2018) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2018.08.023 · Cited by 192 · see citing works
Zitouni, N., Bousserrhıne, N., Missawi, O., Boughattas, I., Chèvre, N., Santos, R., Belbekhouche, S., Alphonse, V., Tisserand, F., Ludivine, B., et al. (2020) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.124055 · Cited by 184 · see citing works
Effects of polyethylene microplastics on the microbiome and metabolism in larval zebrafish
Zhao, Y., Qin, Z., Huang, Z., Bao, Z., Luo, T., & Jin, Y. (2021) · Environmental Pollution · doi:10.1016/j.envpol.2021.117039 · Cited by 183 · see citing works
Microplastics in freshwater fishes: Occurrence, impacts and future perspectives
Parker, B., Andreou, D., Green, I. D., & Britton, J. R. (2021) · Fish and Fisheries · doi:10.1111/faf.12528 · Cited by 179 · see citing works
Priya, S., Burns, M. B., Ward, T., Mars, R. A. T., Adamowicz, E. M., Lock, E. F., Kashyap, P., Knights, D., & Blekhman, R. (2022) · Nature Microbiology · doi:10.1038/s41564-022-01121-z · Cited by 171 · see citing works
Hanachi, P., Karbalaei, S., Walker, T. R., Cole, M., & Hosseini, S. V. (2019) · Environmental Science and Pollution Research · doi:10.1007/s11356-019-05637-6 · Cited by 168 · see citing works
Sarijan, S., Azman, S. B., Said, M. I. M., & Jamal, M. H. (2020) · Environmental Science and Pollution Research · doi:10.1007/s11356-020-11171-7 · Cited by 168 · see citing works
The relationship between the gut microbiome and host gene expression: a review
Nichols, R. G., & Davenport, E. (2020) · Human Genetics · doi:10.1007/s00439-020-02237-0 · Cited by 166 · see citing works
Evidence of Microplastic Ingestion by Fish from the Bahía Blanca Estuary in Argentina, South America
Arias, A. H., Ronda, A. C., Oliva, A. L., & Marcovecchio, J. E. (2019) · Bulletin of Environmental Contamination and Toxicology · doi:10.1007/s00128-019-02604-2 · Cited by 142 · see citing works
Feng, S., Zeng, Y., Cai, Z., Wu, J., Chan, L. L., Zhu, J., & Zhou, J. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.143558 · Cited by 135 · see citing works
Polystyrene microplastics induce metabolic disturbances in marine medaka (Oryzias melastigmas) liver
Ye, G., Zhang, X., Liu, X., Liao, X., Zhang, H., Yan, C., Lin, Y., & Huang, Q. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.146885 · Cited by 130 · see citing works
Analysis and inorganic composition of microplastics in commercial Malaysian fish meals
Karbalaei, S., Golieskardi, A., Watt, D. U., Boiret, M., Hanachi, P., Walker, T. R., & Karami, A. (2019) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2019.110687 · Cited by 119 · see citing works
Zeytin, S., Wagner, G., Mackay-Roberts, N., Gerdts, G., Schuirmann, E., Klockmann, S., & Slater, M. J. (2020) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2020.111210 · Cited by 115 · see citing works
Pathogenicity and Virulence of Legionella : Intracellular replication and host response
Chauhan, D., & Shames, S. R. (2021) · Virulence · doi:10.1080/21505594.2021.1903199 · Cited by 113 · see citing works
Microplastics in fish meals: An exposure route for aquaculture animals
Wang, Q., Li, J., Zhu, X., Sun, C., Teng, J., Chen, L., Shan, E., & Zhao, J. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.151049 · Cited by 97 · see citing works
A comparison of microplastic contamination in freshwater fish from natural and farmed sources
Gómez, Á. G. G., Suárez, D. C., Li, J., & Rotchell, J. M. (2020) · Environmental Science and Pollution Research · doi:10.1007/s11356-020-11605-2 · Cited by 95 · see citing works
Fish out, plastic in: Global pattern of plastics in commercial fishmeal
Gündoğdu, S., Eroldoğan, O. T., Evliyaoğlu, E., Turchini, G. M., & Wu, X. G. (2020) · Aquaculture · doi:10.1016/j.aquaculture.2020.736316 · Cited by 91 · see citing works
Pang, M., Wang, Y., Tang, Y., Dai, J., Tong, J., & Jin, G. (2021) · Environmental Pollution · doi:10.1016/j.envpol.2021.116860 · Cited by 77 · see citing works
Inflammatory Cytokines, Immune Cells, and Organ Interactions in Heart Failure
Li, H., Chen, C., & Wang, D. W. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.695047 · Cited by 72 · see citing works
Lü, X., Deng, D., Huang, F., Casu, F., Kraco, E., Newton, R. J., Zohn, M., Teh, S. J., Watson, A. M., Shepherd, B. S., et al. (2022) · Animal nutrition · doi:10.1016/j.aninu.2022.01.007 · Cited by 72 · see citing works
Self-citationAlomar, C., Sanz‐Martín, M., Compa, M., Rios-Fuster, B., Álvarez, E., Ripolles, V., Valencia, J. M., & Deudero, S. (2021) · Environmental Pollution · doi:10.1016/j.envpol.2021.116960 · Cited by 63 · see citing works
Chlamydiaand Its Many Ways of Escaping the Host Immune System
Wong, W. F., Chambers, J. P., Gupta, R., & Arulanandam, B. P. (2019) · Journal of Pathogens · doi:10.1155/2019/8604958 · Cited by 39 · see citing works
Technical efficiency of different farm sizes for tilapia farming in China
Yuan, Y., Yongming, Y., Dai, Y., Zhang, Z., Gong, Y., & Yuan, Y. (2019) · Aquaculture Research · doi:10.1111/are.14376 · Cited by 31 · see citing works
Dietary calcium requirement of red swamp crayfish ( Procambarus clarkia )
Xu, L., Tian, J., Wen, H., Wu, F., Zhang, W., Gao, W., & Chen, X. (2020) · Aquaculture Nutrition · doi:10.1111/anu.13173 · Cited by 20 · see citing works
Self-citationActinobacteria in indoor environments exposures and respiratory health effects
Rintala, H. (2011) · Frontiers in Bioscience-Scholar · doi:10.2741/225 · Cited by 20 · see citing works
Microcystin-LR-regulated transcriptome dynamics in ZFL cells
Lü, X., Tian, J., Wen, H., Jiang, M., Liu, W., Wu, F., Yu, L., & Zhong, S. (2019) · Aquatic Toxicology · doi:10.1016/j.aquatox.2019.04.018 · Cited by 17 · see citing works
Self-citationActinobacteria in indoor environments exposures and respiratory health effects
Rintala, H. (2011) · Frontiers in Bioscience-Scholar · doi:10.2741/s225 · Cited by 8 · see citing works
Flagged first, then by citations
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Wang, X., Deng, K., Zhang, P., Chen, Q., Magnuson, J. T., Qiu, W., & Zhou, Y. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.170962 · Cited by 118 · see citing works
Ghosh, T. (2024) · Toxicology Reports · doi:10.1016/j.toxrep.2024.101854 · Cited by 115 · see citing works
Dong, R., Zhou, C., Wang, S., Yan, Y., & Jiang, Q. (2022) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2022.09.022 · Cited by 66 · see citing works
Matias, R. S., Gomes, S., Barboza, L. G. A., Salazar-Gutierrez, D., Guilhermino, L. M., & Valente, L. M. P. (2023) · Chemosphere · doi:10.1016/j.chemosphere.2023.139055 · Cited by 52 · see citing works
Zheng, S., Tang, B. Z., & Wang, W. (2023) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2023.133181 · Cited by 51 · see citing works
Wu, D., Lü, X., Dong, L.-X., Tian, J., Deng, J. G., Wei, L., Wen, H., Zhong, S., & Jiang, M. (2023) · Journal of Environmental Sciences · doi:10.1016/j.jes.2023.02.018 · Cited by 45 · see citing works
Self-citationFrom plankton to fish: The multifaceted threat of microplastics in freshwater environments
Gao, C., Xu, B., Li, Z., Wang, Z., Huang, S., Jiang, Z., Gong, X., & Yang, H. (2025) · Aquatic Toxicology · doi:10.1016/j.aquatox.2025.107242 · Cited by 36 · see citing works
Ghosal, S., Bag, S., Rao, S. R., & Bhowmik, S. (2024) · RSC Advances · doi:10.1039/d4ra04544k · Cited by 35 · see citing works
Lu, H., Xu, P., Sun, G., Chen, B., Zheng, Y., Zhang, J., & Wang, G. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.176802 · Cited by 25 · see citing works
Self-citationHuang, J.-N., Gao, C., Ren, H., Wen, B., Wang, Z., Gao, J., & Chen, Z. (2025) · Environment International · doi:10.1016/j.envint.2025.109360 · Cited by 25 · see citing works
Chen, X., Zhang, Z.-D., Hsueh, Y., Zhang, C., Yu, J., Zhu, J., Niu, J., Yin, N., Zhang, J., Cui, X., et al. (2025) · Environmental Research · doi:10.1016/j.envres.2025.120928 · Cited by 23 · see citing works
Self-citationLiu, H., Li, H., Liu, Y., Zhao, H., & Peng, R. (2024) · Environmental Science and Pollution Research · doi:10.1007/s11356-024-35434-9 · Cited by 21 · see citing works
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Robison‐Smith, C., Masud, N., Tarring, E. C., Ward, B. D., & Cable, J. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.168086 · Cited by 21 · see citing works
Rashid, E., Hussain, S. M., Sarker, P. K., Ali, S., & Paray, B. A. (2024) · Aquaculture Reports · doi:10.1016/j.aqrep.2024.102100 · Cited by 20 · see citing works
RetractedTay, D. D., Subbiah, V. K., Shapawi, R., Shah, M. D., Ahmad, H. F., & Mazlan, N. B. (2025) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-025-05330-0 · Cited by 17 · see citing works
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Zheng, Y., Xiaoxian, L., Hu, J., Sun, Y., Zhu, H., & Xu, G. (2024) · Chemosphere · doi:10.1016/j.chemosphere.2024.141644 · Cited by 17 · see citing works
Xu, Y., Liu, L., Ma, Y., Wang, C., Duan, F., Feng, J., Yin, H., Sun, L., Cao, Z., Jung, J., et al. (2024) · Aquatic Toxicology · doi:10.1016/j.aquatox.2024.107215 · Cited by 16 · see citing works
Milne, M. H., Helm, P. A., Munno, K., Bhavsar, S. P., & Rochman, C. M. (2024) · Environmental Health Perspectives · doi:10.1289/ehp13540 · Cited by 15 · see citing works
Zheng, Y., Tang, H., Hu, J., Sun, Y., Zhu, H., & Xu, G. (2024) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2024.117076 · Cited by 15 · see citing works
Gao, T., Jiang, Y., Han, Y., & Wāng, Y. (2025) · Journal of Advanced Research · doi:10.1016/j.jare.2025.06.074 · Cited by 15 · see citing works
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Méndez‐Rodríguez, K. B., Avalos, J. A. J., Fernández-Macías, J. C., & González-Palomo, A. K. (2023) · Environmental Science and Pollution Research · doi:10.1007/s11356-023-30853-6 · Cited by 12 · see citing works
Liu, Y., Teng, X., Chen, L., Wu, S., Xue, C., & Li, Z. (2024) · Foods · doi:10.3390/foods13050765 · Cited by 12 · see citing works
Muhib, M. I., & Rahman, M. M. (2024) · Environmental Science and Pollution Research · doi:10.1007/s11356-024-33782-0 · Cited by 12 · see citing works
Capuano, N., Lombardi, M., Cafà, N., Marino, M., Salzano, F., Scalia, F., Marfella, R., Villone, G., Cappello, F., Szychlinska, M. A., et al. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27073272 · Cited by 11 · see citing works
Zheng, S., & Wang, W. (2025) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2025.137471 · Cited by 11 · see citing works
Liu, S. Y., Li, D. L., Zhu, R., Meng, S. T., Wang, Y. T., Li, L., Yang, Z. Y., & Wu, L. (2024) · Chemosphere · doi:10.1016/j.chemosphere.2024.143676 · Cited by 10 · see citing works
Zheng, Y., Li, J., Gao, J., Jin, W., Hu, J., Sun, Y., Zhu, H., & Xu, G. (2024) · Comparative Biochemistry and Physiology Part D Genomics and Proteomics · doi:10.1016/j.cbd.2024.101370 · Cited by 10 · see citing works
Fang, S., Huang, Y., Xiang, Z., Rong, Z., Zeng, S., & Liu, S. (2023) · Environmental Science Nano · doi:10.1039/d2en00982j · Cited by 10 · see citing works
Sun, Z., Zhao, L., Peng, X., Yan, M., Ding, S., Sun, J., & Kang, B. (2024) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2024.116175 · Cited by 10 · see citing works
Matias, R. S., Monteiro, M., Sousa, V., Pinho, B., Guilhermino, L. M., Valente, L. M. P., & Gomes, S. (2024) · Environmental Research · doi:10.1016/j.envres.2024.120208 · Cited by 9 · see citing works
Zhang, P., Lu, G., Zhang, L., Yan, Z., Zhang, J., & Ding, K. (2025) · Journal of Environmental Management · doi:10.1016/j.jenvman.2025.126711 · Cited by 9 · see citing works
Self-citationFrolova, S. G., Vatlin, A. A., Pospelova, I., Mitkin, N. A., Kulieva, G. A., & Pavshintsev, V. V. (2025) · Toxics · doi:10.3390/toxics13090769 · Cited by 9 · see citing works
Bukhari, M. N., Fatima, M., Wang, P., Jin, Z., Hu, Z., Dar, M. A., Gao, M., Taqi, M., Zhang, P., & Wang, B. (2025) · Separation and Purification Technology · doi:10.1016/j.seppur.2025.134739 · Cited by 8 · see citing works
MICROPLASTICS CONTAMINATION IN FRESHWATER FISH – A REVIEW
Hayat, H., Mubeen, F., Fatima, M., Fatima, I., & *, S. I. A. S. A. (2025) · Agrobiological Records · doi:10.47278/journal.abr/2025.005 · Cited by 7 · see citing works
de Oliveira, C. G., Boaventura, T. P., dos Santos, F. A. C., Souza, A. D. S., Silva, S. D. S., Gil, C. S. B., Luz, R. K., & Oréfice, R. L. (2025) · Aquatic Toxicology · doi:10.1016/j.aquatox.2025.107338 · Cited by 7 · see citing works
Zhi, L., Li, R., Li, Z., Su, Z. W., Chen, F., Qin, Q., Huang, Y., Huang, X., & Wang, J. (2025) · Environmental Chemistry and Ecotoxicology · doi:10.1016/j.enceco.2025.07.009 · Cited by 7 · see citing works
Impact of micro and nanoplastics on aquaculture animals: a systematic review
Mohsen, M., Gündoğdu, S., Wang, Q., Zhou, H., & Zhang, C. (2025) · Aquaculture International · doi:10.1007/s10499-025-02323-y · Cited by 7 · see citing works
Microplastics Induced Dysfunctions in Physiology and Behavior of Fish: A Comprehensive Review
Siddique, M. A. U. Z., Sheikh, M. S., Shahjahan, M., & Al-Emran, M. (2026) · Journal of Toxicology · doi:10.1155/jt/4669316 · Cited by 6 · see citing works
Wu, D., Lü, X., Dong, L.-X., Tian, J., Wen, H., Zhong, S., Li, Y., & Jiang, M. (2024) · Reviews in Fish Biology and Fisheries · doi:10.1007/s11160-024-09855-1 · Cited by 6 · see citing works
Self-citationWang, Y., Shang, Y., Guo, J., Xu, G., Lu, G., Zhou, X. C., & Liu, J. (2025) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2025.139212 · Cited by 6 · see citing works
Wu, K., Liao, W.-R., Lin, W., & Chen, S. (2023) · Separations · doi:10.3390/separations10040257 · Cited by 6 · see citing works
Chen, X., Zhang, Y., Huang, Z., Bi, B., & Wang, X. (2025) · Food Chemistry · doi:10.1016/j.foodchem.2025.144053 · Cited by 5 · see citing works
Uzochukwu, I. E., Nagy, L., Somogyi, D., Pásztor, A., Ossai, N. I., Antal, L., Yancheva, V., Csarnovics, I., & Nyeste, K. (2026) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2026.141048 · Cited by 5 · see citing works
Matias, R. S., Holhorea, P. G., Gomes, S. M., Calduch-Giner, J. À., Guilhermino, L. M., Naya-Català, F., Pérez‐Sánchez, J., & Valente, L. M. P. (2025) · Environmental Pollution · doi:10.1016/j.envpol.2025.127084 · Cited by 5 · see citing works
The Presence of Microplastics in the Genus Oreochromis: A Review
Mendoza-López, D. G., Castañeda‐Chávez, M. D. R., Martinez-Cardenas, L., Castillo-Márquez, E. F., & Lango‐Reynoso, F. (2025) · Microplastics · doi:10.3390/microplastics4020029 · Cited by 3 · see citing works
Loganathan, T., Dyke, J., & Volkoff, H. (2025) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpa.2025.111935 · Cited by 3 · see citing works
Ephsy, D. K., & Selvaraju, R. (2025) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2025.140860 · Cited by 3 · see citing works
Zhang, P., Lu, G., Zhang, L., Yan, Z., & Wang, M. (2025) · Environmental Pollution · doi:10.1016/j.envpol.2025.127579 · Cited by 3 · see citing works
Self-citationJing, W., Zheng, M., Yang, X., He, B., Zhang, X., & Cui, W. (2026) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2026.120278 · Cited by 2 · see citing works
Metabonomic Analysis of Macrobrachium rosenbergii with Iron Prawn Syndrome (IPS)
Li, X., Shen, P.-J., Jiang, W., Meng, J., Cheng, H., & Gao, Q. (2023) · Fishes · doi:10.3390/fishes8040196 · Cited by 2 · see citing works
Al-Jandal, N. J., Azad, I. S., Alkhubaizi, A., Akbar, A., Al-Hasan, E., Hussain, S., & Al-Mansour, H. (2025) · Current Issues in Molecular Biology · doi:10.3390/cimb47090701 · Cited by 1 · see citing works
Chen, T., Ye, P., Yuan, C., Fang, L., Li, N., Chen, G., Qiu, J., Zhu, F., & Ouyang, G. (2025) · Advances in Sample Preparation · doi:10.1016/j.sampre.2025.100212 · Cited by 1 · see citing works
Gautam, R. K., Singh, M., Ali, B., Sharma, N., Sahu, A., & Mishra, A. (2026) · Frontiers in Aquaculture · doi:10.3389/faquc.2026.1771660 · Cited by 1 · see citing works
Janamala, R. P., Kolandhsamy, P., Thondapu, K., Pillam, U. K., Nagari, S., & Kumar, J. A. (2026) · Environmental Pollution and Management · doi:10.1016/j.epm.2026.06.005 · Cited by 1 · see citing works
Omics approaches in fish nutrition research
Martín, S., Porter, D., Mente, E., Casu, F., & Watson, A. M. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-21556-8.00014-4 · Cited by 1 · see citing works
Transcriptional biomarkers of microplastic exposure in fish: a tissue-resolved scoping review
Bračun, S., & Ahi, E. P. (2026) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2026.120301 · Cited by 1 · see citing works
Effect of Natural Particles on the Growth and Behavior of Sebastes Schlegelii
Diao, W., & Liu, L. (2023) · Journal of Physics Conference Series · doi:10.1088/1742-6596/2463/1/012058
Zhang, C., Mao, C., Gao, Q., Shen, W., Zhang, X., & Lin, D. (2026) · Environmental Science Nano · doi:10.1039/d6en00242k
Huo, W., Wang, M., Han, Y., Weng, K., & Xia, X. (2026) · Current Research in Food Science · doi:10.1016/j.crfs.2026.101516
Zeng, Y., Shen, X., Xu, P., Zhou, X., Zhang, X., Zhang, X., Wang, C., & Yan, B. (2026) · Environmental Science Nano · doi:10.1039/d6en00434b
Wang, R., Tang, Y., Zhang, B., Wang, X., Jiang, Y., Sun, H., Ren, J., & Liu, S. (2026) · Environment & Health · doi:10.1021/envhealth.6c00295
Hou, W., Zhao, S., Kong, H., Gao, H., Luo, H., & Ye, H. (2026) · Marine Biotechnology · doi:10.1007/s10126-026-10692-5
Microplastics and living organisms: Are they at risk?
Rovira, J., Barrientos‐Riosalido, A., Marı́, M., González, N., Llopart, J. S., Papiol, G. G., Carafa, R., & Expósito, N. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-45158-4.00010-4
The Menace of Microplastics and Their Physiological Implications in Aquaculture
Ranjan, A., Sriranjani, K., Uma, A., Thangarani, A. J., & Kathiravan, R. (2026) · Microplastics · doi:10.3390/microplastics5040185
Yoon, H. M., Shim, B. S., An, J.-H., Lee, D.-Y., Heum Gi, P. A. R. K., Chen, Q., Park, H. J., Lee, J.-S., Lee, S.-M., & Lee, Y. H. (2026) · Journal of Hazardous Materials Advances · doi:10.1016/j.hazadv.2026.101673
Presence of Microplastics in Tilapia Culture Water
Mendoza-López, D. G., Chávez, M. D. R. C., & Lango‐Reynoso, F. (2026) · Agro Productividad · doi:10.32854/a404e907
- Authors
- Lu, X., Zhang, J.-X., Zhang, L., Wu, D., Tian, J., Yu, L.-J., He, L., Zhong, S., Du, H., Deng, D.-F., Ding, Y.-Z., Wen, H., & Jiang, M.
- Affiliations
- Ministry of Agriculture and Rural Affairs (CN)
- Wuhan University (CN)
- University of Wisconsin–Milwaukee (US)
- Corresponding author
- Wen, Hua
- Jiang, Ming
- Venue
- Science of The Total Environment
- Publisher
- Elsevier BV
- Published
- 2022-10
- Volume
- 841
- Pages
- 156571
- ISSN
- 0048-9697 (ISSN-L)
- OpenAlex
- W4281633384
- Cited by
- 65
- References
- 72
- Citations by year
- 2026: 15 · 2025: 23 · 2024: 17 · 2023: 9 · 2022: 1
- FWCI
- 2.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- The Science of The Total Environment (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31872554, 32172952)
- Chinese Academy of Fishery Sciences (2020JBF01, 2020JBF09, 2020TD74, YFI202213)
- Agriculture Research System of China (CARS-46, CARS-48)
- National Key Research and Development Program of China (2018YFD0900400)
- Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences
- Topics
- Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Keywords
- polyethylene microplastics, dietary microplastic exposure, genetically improved farmed tilapia, Oreochromis niloticus, fish growth, gut microbiota, hepatic metabolism, particle size effects, photoperiodism, circadian rhythm, TCA cycle, propanoate metabolism, pyruvate metabolism, immune pathways
- MeSH terms
- Gastrointestinal Microbiome, Dietary Exposure, Microplastics, Animals, Plastics, Gene Expression, Tilapia, Polyethylene, Cichlids
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