The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.
Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi
Open accessAbstract
The balance between stem cell self-renewal and differentiation is precisely controlled to ensure tissue homeostasis and prevent tumorigenesis. Here we use genome-wide transgenic RNAi to identify 620 genes potentially involved in controlling this balance in Drosophila neuroblasts. We quantify all phenotypes and derive measurements for proliferation, lineage, cell size, and cell shape. We identify a set of transcriptional regulators essential for self-renewal and use hierarchical clustering and integration with interaction data to create functional networks for the control of neuroblast self-renewal and differentiation. Our data identify key roles for the chromatin remodeling Brm complex, the spliceosome, and the TRiC/CCT-complex and show that the alternatively spliced transcription factor Lola and the transcriptional elongation factors Ssrp and Barc control self-renewal in neuroblast lineages. As our data are strongly enriched for genes highly expressed in murine neural stem cells, they are likely to provide valuable insights into mammalian stem cell biology as well.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506580102 · Cited by 53,625
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
Dietzl, G., Chen, D., Schnorrer, F., Su, K.-C., Barinova, Y., Fellner, M., Gasser, B., Kinsey, K., Oppel, S., Scheiblauer, S., et al. (2007) · Nature · doi:10.1038/nature05954 · Cited by 2,644
Lee, T., & Luo, L. (1999) · Neuron · doi:10.1016/s0896-6273(00)80701-1 · Cited by 2,571
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells
Ramalho-Santos, M., Yoon, S., Matsuzaki, Y., Mulligan, R. C., & Melton, D. A. (2002) · Science · doi:10.1126/science.1072530 · Cited by 1,709
Using FlyAtlas to identify better Drosophila melanogaster models of human disease
Chintapalli, V. R., Wang, J., & Dow, J. A. T. (2007) · Nature Genetics · doi:10.1038/ng2049 · Cited by 1,472
Converging concepts of protein folding in vitro and in vivo
Hartl, F. U., & Hayer-Hartl, M. (2009) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1591 · Cited by 1,185
The Tumor Suppressor p53 Regulates Polarity of Self-Renewing Divisions in Mammary Stem Cells
Cicalese, A., Bonizzi, G., Pasi, C. E., Faretta, M., Ronzoni, S., Giulini, B., Brisken, C., Minucci, S., Di Fiore, P. P., & Pelicci, P. G. (2009) · Cell · doi:10.1016/j.cell.2009.06.048 · Cited by 696
The timing of cortical neurogenesis is encoded within lineages of individual progenitor cells
Shen, Q., Wang, Y., Dimos, J. T., Fasano, C. A., Phoenix, T. N., Lemischka, I. R., Ivanova, N. B., Stifani, S., Morrisey, E. E., & Temple, S. (2006) · Nature Neuroscience · doi:10.1038/nn1694 · Cited by 625
The SWI/SNF complex and cancer
Reisman, D., Glaros, S., & Thompson, E. A. (2009) · Oncogene · doi:10.1038/onc.2009.4 · Cited by 568
Betschinger, J., Mechtler, K., & Knoblich, J. A. (2006) · Cell · doi:10.1016/j.cell.2006.01.038 · Cited by 524
Self-citationLoss of negative regulation by Numb over Notch is relevant to human breast carcinogenesis
Pece, S., Serresi, M., Santolini, E., Capra, M., Hulleman, E., Galimberti, V., Zurrida, S., Maisonneuve, P., Viale, G., & Di Fiore, P. P. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200406140 · Cited by 483
Induction of tumor growth by altered stem-cell asymmetric division in Drosophila melanogaster
Caussinus, E., & Gonzalez, C. (2005) · Nature Genetics · doi:10.1038/ng1632 · Cited by 450
The Tumor Suppressors Brat and Numb Regulate Transit-Amplifying Neuroblast Lineages in Drosophila
Bowman, S. K., Rolland, V., Betschinger, J., Kinsey, K. A., Emery, G., & Knoblich, J. A. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.03.004 · Cited by 444
Self-citationNeural stem cells: balancing self-renewal with differentiation
Doe, C. Q. (2008) · Development · doi:10.1242/dev.014977 · Cited by 420
Schwamborn, J. C., Berezikov, E., & Knoblich, J. A. (2009) · Cell · doi:10.1016/j.cell.2008.12.024 · Cited by 414
Self-citationDividing cellular asymmetry: asymmetric cell division and its implications for stem cells and cancer
Neumüller, R. A., & Knoblich, J. A. (2009) · Genes & Development · doi:10.1101/gad.1850809 · Cited by 410
Self-citationChoksi, S. P., Southall, T. D., Bossing, T., Edoff, K., de Wit, E., Fischer, B. E., van Steensel, B., Micklem, G., & Brand, A. H. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.09.015 · Cited by 410
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal
Hu, G., Kim, J., Xu, Q., Leng, Y., Orkin, S. H., & Elledge, S. J. (2009) · Genes & Development · doi:10.1101/gad.1769609 · Cited by 393
Fisher, E. M. C., Beer-Romero, P., Brown, L. G., Ridley, A., McNeil, J. A., Lawrence, J. B., Willard, H. F., Bieber, F. R., & Page, D. C. (1990) · Cell · doi:10.1016/0092-8674(90)90416-c · Cited by 393
Functional Specificity among Ribosomal Proteins Regulates Gene Expression
Komili, S., Farny, N. G., Roth, F. P., & Silver, P. A. (2007) · Cell · doi:10.1016/j.cell.2007.08.037 · Cited by 390
Bello, B. C., Izergina, N., Caussinus, E., & Reichert, H. (2008) · Neural Development · doi:10.1186/1749-8104-3-5 · Cited by 388
The ribosomal protein genes and Minute loci of Drosophila melanogaster
Marygold, S. J., Roote, J., Reuter, G., Lambertsson, A., Ashburner, M., Millburn, G. H., Harrison, P. M., Yu, Z., Kenmochi, N., Kaufman, T. C., et al. (2007) · Genome Biology · doi:10.1186/gb-2007-8-10-r216 · Cited by 385
The Endocytic Protein α-Adaptin Is Required for Numb-Mediated Asymmetric Cell Division in Drosophila
Berdnik, D., Török, T., González-Gaitán, M., & Knoblich, J. A. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00215-0 · Cited by 366
Self-citationBoone, J. Q., & Doe, C. Q. (2008) · Developmental Neurobiology · doi:10.1002/dneu.20648 · Cited by 366
Drosophila Brain Tumor is a translational repressor
Sonoda, J., & Wharton, R. P. (2001) · Genes & Development · doi:10.1101/gad.870801 · Cited by 329
Lee, C.-Y., Wilkinson, B. D., Siegrist, S. E., Wharton, R. P., & Doe, C. Q. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.01.017 · Cited by 323
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi
Mummery-Widmer, J. L., Yamazaki, M., Stoeger, T., Novatchkova, M., Bhalerao, S., Chen, D., Dietzl, G., Dickson, B. J., & Knoblich, J. A. (2009) · Nature · doi:10.1038/nature07936 · Cited by 305
Self-citationActin-Capping Protein and the Hippo pathway regulate F-actin and tissue growth in Drosophila
Fernández, B. G., Gaspar, P., Brás-Pereira, C., Jezowska, B., Rebelo, S. R., & Janody, F. (2011) · Development · doi:10.1242/dev.063545 · Cited by 293
Identification of alternative splicing regulators by RNA interference in Drosophila
Park, J. W., Parisky, K., Celotto, A. M., Reenan, R. A., & Graveley, B. R. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0407004101 · Cited by 290
Papoulas, O., Beek, S. J., Moseley, S. L., McCallum, C. M., Sarte, M., Shearn, A., & Tamkun, J. W. (1998) · Development · doi:10.1242/dev.125.20.3955 · Cited by 286
Bello, B., Reichert, H., & Hirth, F. (2006) · Development · doi:10.1242/dev.02429 · Cited by 276
Role of Drosophila α-Adaptin in Presynaptic Vesicle Recycling
González-Gaitán, M., & Jäckle, H. (1997) · Cell · doi:10.1016/s0092-8674(00)81923-6 · Cited by 266
Ding, L., Paszkowski-Rogacz, M., Nitzsche, A., Slabicki, M. M., Heninger, A.-K., Vries, I. D., Kittler, R., Junqueira, M., Shevchenko, A., Schulz, H., et al. (2009) · Cell Stem Cell · doi:10.1016/j.stem.2009.03.009 · Cited by 261
Gradients of the Drosophila Chinmo BTB-Zinc Finger Protein Govern Neuronal Temporal Identity
Zhu, S., Lin, S., Kao, C.-F., Awasaki, T., Chiang, A.-S., & Lee, T. (2006) · Cell · doi:10.1016/j.cell.2006.08.045 · Cited by 248
Mei-P26 regulates microRNAs and cell growth in the Drosophila ovarian stem cell lineage
Neumüller, R. A., Betschinger, J., Fischer, A., Bushati, N., Poernbacher, I., Mechtler, K., Cohen, S. M., & Knoblich, J. A. (2008) · Nature · doi:10.1038/nature07014 · Cited by 239
Self-citationTIF1γ Controls Erythroid Cell Fate by Regulating Transcription Elongation
Bai, X., Kim, J., Yang, Z., Jurynec, M. J., Akie, T. E., Lee, J., LeBlanc, J., Sessa, A., Jiang, H., DiBiase, A., et al. (2010) · Cell · doi:10.1016/j.cell.2010.05.028 · Cited by 212
Epigenetic silencers and Notch collaborate to promote malignant tumours by Rb silencing
Ferres-Marco, D., Gutierrez-Garcia, I., Vallejo, D. M., Bolivar, J., Gutierrez-Aviño, F. J., & Dominguez, M. (2006) · Nature · doi:10.1038/nature04376 · Cited by 206
Petrova, T. V., Nykänen, A., Norrmén, C., Ivanov, K. I., Andersson, L. C., Haglund, C., Puolakkainen, P., Wempe, F., von Melchner, H., Gradwohl, G., et al. (2008) · Cancer Cell · doi:10.1016/j.ccr.2008.02.020 · Cited by 192
A Novel, Evolutionarily Conserved Protein Phosphatase Complex Involved in Cisplatin Sensitivity
Gingras, A.-C., Caballero, M., Zarske, M., Sanchez, A., Hazbun, T. R., Fields, S., Sonenberg, N., Hafen, E., Raught, B., & Aebersold, R. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500231-mcp200 · Cited by 186
RPG: the Ribosomal Protein Gene database
Nakao, A. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh004 · Cited by 178
Schwabish, M. A., & Struhl, K. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.00717-07 · Cited by 158
Stem Cell Self-Renewal Controlled by Chromatin Remodeling Factors
Xi, R., & Xie, T. (2005) · Science · doi:10.1126/science.1120140 · Cited by 152
ATP-dependent chromatin remodeling in neural development
Yoo, A. S. J., & Crabtree, G. R. (2009) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2009.04.006 · Cited by 151
Bmi-1 cooperates with Foxg1 to maintain neural stem cell self-renewal in the forebrain
Fasano, C. A., Phoenix, T. N., Kokovay, E., Lowry, N., Elkabetz, Y., Dimos, J. T., Lemischka, I. R., Studer, L., & Temple, S. (2009) · Genes & Development · doi:10.1101/gad.1743709 · Cited by 146
Tat-SF1: Cofactor for Stimulation of Transcriptional Elongation by HIV-1 Tat
Zhou, Q., & Sharp, P. A. (1996) · Science · doi:10.1126/science.274.5287.605 · Cited by 143
The expression profile of purified Drosophila germline stem cells
Kai, T., Williams, D., & Spradling, A. C. (2005) · Developmental Biology · doi:10.1016/j.ydbio.2005.04.018 · Cited by 134
Neural stem cell transcriptional networks highlight genes essential for nervous system development
Southall, T. D., & Brand, A. H. (2009) · The EMBO Journal · doi:10.1038/emboj.2009.309 · Cited by 134
Martin-Bermudo, M. D., Martı́nez, C., Rodrı́guez, A., & Jiménez, F. (1991) · Development · doi:10.1242/dev.113.2.445 · Cited by 134
Facts about FACT and transcript elongation through chromatin
Belotserkovskaya, R., & Reinberg, D. (2004) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2004.02.004 · Cited by 133
lola encodes a putative transcription factor required for axon growth and guidance in Drosophila
Giniger, E., Tietje, K. M., Jan, L. Y., & Jan, Y. N. (1994) · Development · doi:10.1242/dev.120.6.1385 · Cited by 132
陳, 業., Yamaguchi, Y., 山口, 雄., Yamamoto, J., 山本, 淳., Nakamura, M., 中村, 光., & Handa, H. (2009) · Genes & Development · doi:10.1101/gad.1834709 · Cited by 129
Helps, N. R., Brewis, N., Lineruth, K., et al. (1998) · Journal of Cell Science · doi:10.1242/jcs.111.10.1331 · Cited by 123
Two-step process for photoreceptor formation in Drosophila
Mollereau, B., Domínguez, M., Webel, R., Colley, N., Keung, B., de Celis, J. D., & Desplan, C. (2001) · Nature · doi:10.1038/35091076 · Cited by 116
Liu, L., Qi, H., Wang, J., & Lin, H. (2011) · Development · doi:10.1242/dev.059287 · Cited by 109
The EH1 motif in metazoan transcription factors
Copley, R. R. (2005) · BMC Genomics · doi:10.1186/1471-2164-6-169 · Cited by 91
Adryan, B., & Teichmann, S. A. (2006) · Bioinformatics · doi:10.1093/bioinformatics/btl143 · Cited by 90
Goeke, S., Greene, E. A., Grant, P. K., Gates, M. A., Crowner, D., Aigaki, T., & Giniger, E. (2003) · Nature Neuroscience · doi:10.1038/nn1105 · Cited by 87
Bello, B. C., Holbro, N., & Reichert, H. (2007) · Development · doi:10.1242/dev.02793 · Cited by 75
Pfreundt, U., James, D. P., Tweedie, S., Wilson, D. A., Teichmann, S. A., & Adryan, B. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp910 · Cited by 74
FF Domains of CA150 Bind Transcription and Splicing Factors through Multiple Weak Interactions
Smith, M. J., Kulkarni, S., & Pawson, T. J. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.21.9274-9285.2004 · Cited by 70
Loss of heterozygosity of TRIM3 in malignant gliomas
Boulay, J., Stiefel, U., Taylor, E., et al. (2009) · BMC Cancer · doi:10.1186/1471-2407-9-71 · Cited by 55
Davis, R. J., Tavsanli, B. C., Dittrich, C., Walldorf, U., & Mardon, G. (2003) · Developmental Biology · doi:10.1016/s0012-1606(03)00201-x · Cited by 55
Ren, J., Jegga, A. G., Zhang, M., Deng, J., Liu, J., Gordon, C. B., Aronow, B. J., Lu, L. J., Zhang, B., & Ma, J. (2011) · Human Molecular Genetics · doi:10.1093/hmg/ddr251 · Cited by 42
Yki/YAP, Sd/TEAD and Hth/MEIS Control Tissue Specification in the Drosophila Eye Disc Epithelium
Zhang, T., Zhou, Q., & Pignoni, F. (2011) · PLoS ONE · doi:10.1371/journal.pone.0022278 · Cited by 39
Transcriptional signature of an adult brain tumor in Drosophila
Loop, T., Leemans, R., Stiefel, U., Hermida, L. C., Egger, B., Xie, F., Primig, M., Certa, U., Fischbach, K., Reichert, H., et al. (2004) · BMC Genomics · doi:10.1186/1471-2164-5-24 · Cited by 36
Bhalerao, S., Berdnik, D., Tőrők, T., & Knoblich, J. A. (2005) · Current Biology · doi:10.1016/j.cub.2005.07.061 · Cited by 32
Self-citationLola regulates cell fate by antagonizing Notch induction in the Drosophila eye
Zheng, L., & Carthew, R. W. (2007) · Mechanisms of Development · doi:10.1016/j.mod.2007.10.007 · Cited by 31
Suissa, Y., Oren, Z., Dinur, T., Arama, E., & Gerlitz, O. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.193235 · Cited by 21
Xie, X., Dubrovskaya, V. A., & Dubrovsky, E. B. (2010) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2010.12.001 · Cited by 17
Kruppel-Like Factors 4 and 5: Unity in Diversity
Sur, I. (2009) · Current Genomics · doi:10.2174/138920209789503932 · Cited by 10
Homeostasis of theDrosophilaadult retina by Actin-Capping Protein and the Hippo pathway
Brás‐Pereira, C., Zhang, T., Pignoni, F., & Janody, F. (2011) · Communicative & Integrative Biology · doi:10.4161/cib.16853 · Cited by 9
Cited by
Drosophila neuroblasts: a model for stem cell biology
Homem, C. C. F., & Knoblich, J. A. (2012) · Development · doi:10.1242/dev.080515 · Cited by 476
Self-citationHomem, C. C. F., Steinmann, V., Burkard, T. R., Jais, A., Esterbauer, H., & Knoblich, J. A. (2014) · Cell · doi:10.1016/j.cell.2014.06.024 · Cited by 243
Self-citationRNA Interference (RNAi) Screening in Drosophila
Heigwer, F., Port, F., & Boutros, M. (2018) · Genetics · doi:10.1534/genetics.117.300077 · Cited by 125
Conserved regulators of nucleolar size revealed by global phenotypic analyses
Neumüller, R. A., Gross, T., Samsonova, A. A., Vinayagam, A., Buckner, M., Founk, K., Hu, Y., Sharifpoor, S., Rosebrock, A. P., Andrews, B., et al. (2013) · Science Signaling · doi:10.1126/scisignal.2004145 · Cited by 87
Self-citationSpliceosome Malfunction Causes Neurodevelopmental Disorders with Overlapping Features
Li, D., Wang, Q., Bayat, A., Battig, M. R., Zhou, Y., Bosch, D. G. M., van Haaften, G., Granger, L., Petersen, A. K., Pérez-Jurado, L. A., et al. (2024) · Journal of Clinical Investigation · doi:10.1172/jci171235 · Cited by 46
Metadata
- Authors
- Neumüller, R. A., Richter, C., Fischer, A., Novatchkova, M., Neumüller, K. G., & Knoblich, J. A.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2011-05
- Volume
- 8
- Issue
- 5
- Pages
- 580-593
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W2082867955
- Cited by
- 255
- References
- 71
- FWCI
- 10.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
