Cited by 346Muscle Physiology and DisordersCongenital heart defects researchDevelopmental Biology and Gene Regulation

Muscle Satellite Cells Are Primed for Myogenesis but Maintain Quiescence with Sequestration of Myf5 mRNA Targeted by microRNA-31 in mRNP Granules

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Crist, Colin G., Montarras, Didier, Buckingham, Margaret·2012·Cell Stem Cell

Abstract

Regeneration of adult tissues depends on stem cells that are primed to enter a differentiation program, while remaining quiescent. How these two characteristics can be reconciled is exemplified by skeletal muscle in which the majority of quiescent satellite cells transcribe the myogenic determination gene Myf5, without activating the myogenic program. We show that Myf5 mRNA, together with microRNA-31, which regulates its translation, is sequestered in mRNP granules present in the quiescent satellite cell. In activated satellite cells, mRNP granules are dissociated, relative levels of miR-31 are reduced, and Myf5 protein accumulates, which initially requires translation, but not transcription. Conditions that promote the continued presence of mRNP granules delay the onset of myogenesis. Manipulation of miR-31 levels affects satellite cell differentiation ex vivo and muscle regeneration in vivo. We therefore propose a model in which posttranscriptional mechanisms hold quiescent stem cells poised to enter a tissue-specific differentiation program.

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Crossref · 10.1016/j.stem.2012.03.011

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  • Myf5 mRNA and its regulatory miRNA miR-31 are sequestered in mRNP granules in quiescent MuSCs.

    “the Myf5 (myogenic factor 5) mRNA and its regulatory miRNA miR-31 were found to be sequestered in mRNA ribonucleoprotein particle (mRNP) granules in quiescent MuSCs91.”

    From Molecular regulation of stem cell quiescence (2013) · cites this paper as [91]

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Authors
Crist, C. G., Montarras, D., & Buckingham, M.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Buckingham, Margaret
Venue
Cell Stem Cell
Publisher
Elsevier BV
Published
2012-07
Volume
11
Issue
1
Pages
118-126
ISSN
  • 1875-9777 (ISSN-L)
  • 1934-5909
OpenAlex
W1993085259
Cited by
346
References
27
Citations by year
2026: 13 · 2025: 16 · 2024: 19 · 2023: 16 · 2022: 28 · 2021: 26 · 2020: 26 · 2019: 30 · 2018: 28 · 2017: 28 · 2016: 31 · 2015: 38 · 2014: 18 · 2013: 21 · 2012: 6
FWCI
10.66
Type
journal article
Language
en
Open access
Locations
  • Cell stem cell (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
satellite cells, Myf5, miR-31, post-transcriptional regulation, stem cell quiescence, myogenesis, skeletal muscle regeneration, translation repression
MeSH terms
Animals, Cell Differentiation, Cytoplasmic Granules, Gene Expression Regulation, Regeneration, Ribonucleoproteins, RNA, Messenger, Muscle Development, Satellite Cells, Skeletal Muscle, MicroRNAs, Mice, Myogenic Regulatory Factor 5

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