Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo
Open accessAbstract
Modulation of Wnt signaling has untapped potential in regenerative medicine due to its essential functions in stem cell homeostasis. However, Wnt lipidation and Wnt-Frizzled (Fzd) cross-reactivity have hindered translational Wnt applications. Here, we designed and engineered water-soluble, Fzd subtype-specific "next-generation surrogate" (NGS) Wnts that hetero-dimerize Fzd and Lrp6. NGS Wnt supports long-term expansion of multiple different types of organoids, including kidney, colon, hepatocyte, ovarian, and breast. NGS Wnts are superior to Wnt3a conditioned media in organoid expansion and single-cell organoid outgrowth. Administration of Fzd subtype-specific NGS Wnt in vivo reveals that adult intestinal crypt proliferation can be promoted by agonism of Fzd5 and/or Fzd8 receptors, while a broad spectrum of Fzd receptors can induce liver zonation. Thus, NGS Wnts offer a unified organoid expansion protocol and a laboratory "tool kit" for dissecting the functions of Fzd subtypes in stem cell biology.
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Conditioned medium from engineered cell lines producing biologically active growth factors can replace commonly used growth factors such as Wnt3a ligands.
“The use of conditioned medium from engineered cell lines producing biologically active growth factors, e.g., L Wnt-3A, can replace commonly used growth factors, such as Wnt3a ligands80.”
From Organoids (2022) · cites this paper as [80]
Novel surrogate molecules are beginning to emerge as potential substitutes for conditioned medium.
“Thus, novel sorrugate molecules are starting to arise as potential substitutes to condition medium80,81.”
From Organoids (2022) · cites this paper as [80]
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Cites
NIH Image to ImageJ: 25 years of image analysis
Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012) · Nature Methods · doi:10.1038/nmeth.2089 · Cited by 64,638 · see citing works
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339 · see citing works
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204 · see citing works
Self-citationWnt/β-Catenin Signaling: Components, Mechanisms, and Diseases
MacDonald, B. T., Tamai, K., & He, X. (2009) · Developmental Cell · doi:10.1016/j.devcel.2009.06.016 · Cited by 5,583 · see citing works
Wnt/β-Catenin Signaling in Development and Disease
Clevers, H. (2006) · Cell · doi:10.1016/j.cell.2006.10.018 · Cited by 5,568 · see citing works
Self-citationWnt/β-catenin signaling and disease
Clevers, H., & Nusse, R. (2012) · Cell · doi:10.1016/j.cell.2012.05.012 · Cited by 5,554 · see citing works
Self-citationThe Wnt Signaling Pathway in Development and Disease
Logan, C. Y., & Nusse, R. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.20.010403.113126 · Cited by 5,325 · see citing works
Self-citationWnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities
Nusse, R., & Clevers, H. (2017) · Cell · doi:10.1016/j.cell.2017.05.016 · Cited by 4,342 · see citing works
Self-citationSato, T., Stange, D. E., Ferrante, M., Vries, R. G. J., van Es, J. H., van den Brink, S., van Houdt, W. J., Pronk, A., van Gorp, J., Siersema, P. D., et al. (2011) · Gastroenterology · doi:10.1053/j.gastro.2011.07.050 · Cited by 3,753 · see citing works
Modeling Development and Disease with Organoids
Clevers, H. (2016) · Cell · doi:10.1016/j.cell.2016.05.082 · Cited by 3,196 · see citing works
Self-citationProspective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients
van de Wetering, M., Francies, H. E., Francis, J. M., Bounova, G., Iorio, F., Pronk, A., van Houdt, W., van Gorp, J., Taylor-Weiner, A., Kester, L., et al. (2015) · Cell · doi:10.1016/j.cell.2015.03.053 · Cited by 2,408 · see citing works
Self-citationWnt proteins are lipid-modified and can act as stem cell growth factors
Willert, K., Brown, J. D., Danenberg, E., Duncan, A. W., Weissman, I. L., Reya, T., Yates, J. R., & Nusse, R. (2003) · Nature · doi:10.1038/nature01611 · Cited by 2,257 · see citing works
Self-citationOrganoid models of human and mouse ductal pancreatic cancer
Boj, S. F., Hwang, C.-I., Baker, L. A., Chio, I. I. C., Engle, D. D., Corbo, V., Jager, M., Ponz-Sarvise, M., Tiriac, H., Spector, M. S., et al. (2015) · Cell · doi:10.1016/j.cell.2014.12.021 · Cited by 2,150 · see citing works
Self-citationA living biobank of breast cancer organoids captures disease heterogeneity
Sachs, N., de Ligt, J., Kopper, O., Gogola, E., Bounova, G., Weeber, F., Balgobind, A. V., Wind, K., Gracanin, A., Begthel, H., et al. (2018) · Cell · doi:10.1016/j.cell.2017.11.010 · Cited by 1,973 · see citing works
Self-citationDrost, J., & Clevers, H. (2018) · Nature Reviews Cancer · doi:10.1038/s41568-018-0007-6 · Cited by 1,790 · see citing works
Self-citationThe glamour and gloom of glycogen synthase kinase-3
Jope, R. S., & Johnson, G. V. W. (2004) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2003.12.004 · Cited by 1,522 · see citing works
The complex world of WNT receptor signalling
Niehrs, C. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3470 · Cited by 1,481 · see citing works
Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling
de Lau, W., Barker, N., Low, T. Y., Koo, B., Li, V. S. W., Teunissen, H., Kujala, P., Haegebarth, A., Peters, P. J., van de Wetering, M., et al. (2011) · Nature · doi:10.1038/nature10337 · Cited by 1,267 · see citing works
Self-citationModeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids
Matano, M., Date, S., Shimokawa, M., Takano, A., Fujii, M., Ohta, Y., Watanabe, T., Kanai, T., & Sato, T. (2015) · Nature Medicine · doi:10.1038/nm.3802 · Cited by 1,135 · see citing works
Sequential cancer mutations in cultured human intestinal stem cells
Drost, J., van Jaarsveld, R. H., Ponsioen, B., Zimberlin, C., van Boxtel, R., Buijs, A., Sachs, N., Overmeer, R. M., Offerhaus, G. J., Begthel, H., et al. (2015) · Nature · doi:10.1038/nature14415 · Cited by 1,067 · see citing works
Self-citationA functional CFTR assay using primary cystic fibrosis intestinal organoids
Dekkers, J. F., Wiegerinck, C. L., de Jonge, H. R., Bronsveld, I., Janssens, H. M., de Winter-de Groot, K. M., Brandsma, A. M., de Jong, N. W. M., Bijvelds, M. J. C., Scholte, B. J., et al. (2013) · Nature Medicine · doi:10.1038/nm.3201 · Cited by 999 · see citing works
Self-citationStructural basis of Wnt recognition by Frizzled
Janda, C. Y., Waghray, D., Levin, A. M., Thomas, C., & Garcia, K. (2012) · Science · doi:10.1126/science.1222879 · Cited by 880 · see citing works
Self-citationarrow encodes an LDL-receptor-related protein essential for Wingless signalling
Wehrli, M., Dougan, S. T., Caldwell, K. A., O’Keefe, L. V., Schwartz, S. L., Vaizel-Ohayon, D., Schejter, E. D., Tomlinson, A., & DiNardo, S. (2000) · Nature · doi:10.1038/35035110 · Cited by 871 · see citing works
Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids
Hu, H., Gehart, H., Artegiani, B., LÖpez-Iglesias, C., Dekkers, F., Basak, O., van Es, J., Chuva de Sousa Lopes, S. M., Begthel, H., Korving, J., et al. (2018) · Cell · doi:10.1016/j.cell.2018.11.013 · Cited by 837 · see citing works
Self-citationAn organoid platform for ovarian cancer captures intra- and interpatient heterogeneity
Kopper, O., de Witte, C. J., Lõhmussaar, K., Valle-Inclan, J. E., Hami, N., Kester, L., Balgobind, A. V., Korving, J., Proost, N., Begthel, H., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0422-6 · Cited by 834 · see citing works
Self-citationIn Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection
Bartfeld, S., Bayram, T., van de Wetering, M., Huch, M., Begthel, H., Kujala, P., Vries, R., Peters, P. J., & Clevers, H. (2015) · Gastroenterology · doi:10.1053/j.gastro.2014.09.042 · Cited by 820 · see citing works
Self-citationMining the Wnt pathway for cancer therapeutics
Barker, N., & Clevers, H. C. (2006) · Nature Reviews Drug Discovery · doi:10.1038/nrd2154 · Cited by 746 · see citing works
Self-citationSelf-renewing diploid Axin2+ cells fuel homeostatic renewal of the liver
Wang, B., Zhao, L., Fish, M., Logan, C. Y., & Nusse, R. (2015) · Nature · doi:10.1038/nature14863 · Cited by 674 · see citing works
Self-citationKuhnert, F., Davis, C. R., Wang, H.-T., Chu, P., Lee, M. A., Yuan, J., Nusse, R., & Kuo, C. J. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2536800100 · Cited by 638 · see citing works
Self-citationSeino, T., Kawasaki, S., Shimokawa, M., Tamagawa, H., Toshimitsu, K., Fujii, M., Ohta, Y., Matano, M., Nanki, K., Kawasaki, K., et al. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2017.12.009 · Cited by 631 · see citing works
Apc Tumor Suppressor Gene Is the “Zonation-Keeper” of Mouse Liver
Benhamouche, S., Decaens, T., Godard, C., Chambrey, R., Rickman, D. S., Moinard, C., Vasseur-Cognet, M., Kuo, C. J., Kahn, A., Perret, C., et al. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.03.015 · Cited by 543 · see citing works
Self-citationDrost, J., van Boxtel, R., Blokzijl, F., Mizutani, T., Sasaki, N., Sasselli, V., de Ligt, J., Behjati, S., Grolleman, J. E., van Wezel, T., et al. (2017) · Science · doi:10.1126/science.aao3130 · Cited by 461 · see citing works
Self-citationTubuloids derived from human adult kidney and urine for personalized disease modeling
Schutgens, F., Rookmaaker, M. B., Margaritis, T., Rios, A. C., Ammerlaan, C. M. E., Jansen, J., Gijzen, L., Vormann, M. K., Vonk, A. M., Viveen, M. C., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0048-8 · Cited by 450 · see citing works
Self-citationNon-equivalence of Wnt and R-spondin ligands during Lgr5+ intestinal stem-cell self-renewal
Yan, K. S., Janda, C. Y., Chang, J., Zheng, G. X. Y., Larkin, K. A., Luca, V. C., Chia, L. A., Mah, A. T., Han, A. S., Terry, J. M., et al. (2017) · Nature · doi:10.1038/nature22313 · Cited by 446 · see citing works
Self-citationSurrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling
Janda, C. Y., Dang, L. T., You, C., Chang, J., de Lau, W., Zhong, Z. A., Yan, K. S., Marecic, O., Siepe, D., Li, X., et al. (2017) · Nature · doi:10.1038/nature22306 · Cited by 403 · see citing works
Self-citationLeeansyah, E., Ganesh, A., Quigley, M. F., Sönnerborg, A. B., Andersson, J., Hunt, P. W., Somsouk, M., Deeks, S. G., Martin, J. N., Moll, M., et al. (2012) · Blood · doi:10.1182/blood-2012-07-445429 · Cited by 372 · see citing works
Inflammatory Cytokine TNFα Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture
Peng, W. C., Logan, C. Y., Fish, M., Anbarchian, T., Aguisanda, F., Álvarez-Varela, A., Wu, P., Jin, Y., Zhu, J., Li, B., et al. (2018) · Cell · doi:10.1016/j.cell.2018.11.012 · Cited by 331 · see citing works
Self-citationQyang, Y., Martı́n-Puig, S., Chiravuri, M., Chen, S., Xu, H., Bu, L., Jiang, X., Lin, L., Granger, A., Moretti, A., et al. (2007) · Cell stem cell · doi:10.1016/j.stem.2007.05.018 · Cited by 309 · see citing works
Josefsson, L., von Stockenström, S., Faria, N. R., Sinclair, E., Bacchetti, P., Killian, M., Epling, L., Tan, A., Ho, T. N., Lemey, P., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1308313110 · Cited by 276 · see citing works
Buikema, J. W., Lee, S., Goodyer, W. R., Maas, R. G. C., Chirikian, O., Li, G., Miao, Y., Paige, S. L., Lee, D. C., Wu, H., et al. (2020) · Cell stem cell · doi:10.1016/j.stem.2020.06.001 · Cited by 218 · see citing works
Self-citationCrystal structures of the extracellular domain of LRP6 and its complex with DKK1
Cheng, Z., Biechele, T. L., Wei, Z., Morrone, S. R., Moon, R. T., Wang, L., & Xu, W. (2011) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2139 · Cited by 203 · see citing works
The Angiocrine Factor Rspondin3 Is a Key Determinant of Liver Zonation
Rocha, A. S., Vidal, V., Mertz, M., Kendall, T. J., Charlet, A., Okamoto, H., & Schedl, A. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.10.049 · Cited by 194 · see citing works
Dijksterhuis, J. P., Baljinnyam, B., Stanger, K., Sercan, H. O., Ji, Y., Andres, O., Rubin, J. S., Hannoush, R. N., & Schulte, G. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.612648 · Cited by 184 · see citing works
Yu, H. M., Ye, X., Guo, N., & Nathans, J. (2012) · Development · doi:10.1242/dev.083352 · Cited by 146 · see citing works
Hunt, P. W., Shulman, N. S., Hayes, T. L., Dahl, V., Somsouk, M., Funderburg, N., McLaughlin, B. E., LANDAY, A. L., Adeyemi, O. M., Gilman, L. T., et al. (2013) · Blood · doi:10.1182/blood-2012-06-436345 · Cited by 138 · see citing works
Boj, S. F., Vonk, A. M., Statia, M., Su, J., Dekkers, J. F., Vries, R., Beekman, J. M., & Clevers, H. C. (2017) · Journal of Visualized Experiments · doi:10.3791/55159 · Cited by 132 · see citing works
Self-citationMapping of Wnt‐Frizzled interactions by multiplex CRISPR targeting of receptor gene families
Voloshanenko, O., Gmach, P., Winter, J., Kranz, D., & Boutros, M. (2017) · The FASEB Journal · doi:10.1096/fj.201700144r · Cited by 118 · see citing works
Tao, Y., Mis, M., Blazer, L. L., Ustav, M., Steinhart, Z., Chidiac, R., Kubarakos, E., O’Brien, S., Wang, X., Jarvik, N., et al. (2019) · eLife · doi:10.7554/elife.46134 · Cited by 106 · see citing works
Prospective Antiretroviral Treatment of Asymptomatic, HIV-1 Infected Controllers
Hatano, H., Yukl, S. A., Ferre, A. L., Graf, E. H., Somsouk, M., Sinclair, E., Abdel‐Mohsen, M., Liegler, T. J., Harvill, K., Hoh, R., et al. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003691 · Cited by 101 · see citing works
Bandaranayake, A. D., Correnti, C., Ryu, B. Y., Brault, M., Strong, R. K., & Rawlings, D. J. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr706 · Cited by 90 · see citing works
Yukl, S. A., Sinclair, E., Somsouk, M., Hunt, P. W., Epling, L., Killian, M., Girling, V., Li, P., Havlir, D. V., Deeks, S. G., et al. (2013) · AIDS · doi:10.1097/qad.0000000000000166 · Cited by 74 · see citing works
Chen, H., Lu, C., Ouyang, B., Zhang, H., Huang, Z. X., Bhatia, D., Lee, S., Shah, D., Sura, A., Yeh, W., et al. (2020) · Cell chemical biology · doi:10.1016/j.chembiol.2020.02.009 · Cited by 74 · see citing works
Buikema, J. W., Mady, A. S. A., Mittal, N., Atmanli, A., Caron, L., Doevendans, P. A., Sluijter, J. P. G., & Domian, I. J. (2013) · Development · doi:10.1242/dev.099325 · Cited by 71 · see citing works
Self-citationSomsouk, M., Dunham, R. M., Cohen, M. L., Albright, R. G., Abdel‐Mohsen, M., Liegler, T. J., Lifson, J. D., Piatak, M., Gorelick, R. J., Huang, Y., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0116306 · Cited by 67 · see citing works
Hatano, H., Somsouk, M., Sinclair, E., Harvill, K., Gilman, L. T., Cohen, M., Hoh, R., Hunt, P. W., Martin, J. N., Wong, J. K., et al. (2013) · AIDS · doi:10.1097/qad.0b013e328362692f · Cited by 54 · see citing works
Receptor subtype discrimination using extensive shape complementary designed interfaces
Dang, L. T., Miao, Y., Ha, A., Yuki, K., Park, K., Janda, C. Y., Jude, K. M., Mohan, K., Ha, N.-T., Vallon, M., et al. (2019) · Nature Structural & Molecular Biology · doi:10.1038/s41594-019-0224-z · Cited by 52 · see citing works
Self-citationWnt acylation and its functional implication in Wnt signalling regulation
Janda, C. Y., & García, K. C. (2015) · Biochemical Society Transactions · doi:10.1042/bst20140249 · Cited by 49 · see citing works
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Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,001 · see citing works
Patient-derived organoids as a predictive biomarker for treatment response in cancer patients
Wensink, G. E., Elias, S. G., Mullenders, J., Koopman, M., Boj, S. F., Kranenburg, O. W., & Roodhart, J. M. L. (2021) · npj Precision Oncology · doi:10.1038/s41698-021-00168-1 · Cited by 323 · see citing works
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Geurts, M. H., Gandhi, S., Boretto, M. G., Akkerman, N., Derks, L. L. M., van Son, G., Celotti, M., Harshuk-Shabso, S., Peci, F., Begthel, H., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-40701-3 · Cited by 66 · see citing works
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Fei, K., Zhang, J., Yuan, J., & Xiao, P. (2022) · Bioengineering · doi:10.3390/bioengineering9030121 · Cited by 65 · see citing works
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- Authors
- Miao, Y., Ha, A., de Lau, W., Yuki, K., Santos, A. J. M., You, C., Geurts, M. H., Puschhof, J., Pleguezuelos-Manzano, C., Peng, W. C., Senlice, R., Piani, C., Buikema, J. W., Gbenedio, O. M., Vallon, M., Yuan, J., de Haan, S., Hemrika, W., Rösch, K., Dang, L. T., Baker, D., Ott, M., Depeille, P., Wu, S. M., Drost, J., Nusse, R., Roose, J. P., Piehler, J., Boj, S. F., Janda, C. Y., Clevers, H., Kuo, C. J., & Garcia, K. C.
- Affiliations
- Howard Hughes Medical Institute (US)
- Stanford Medicine (US)
- Osnabrück University (DE)
- University Medical Center Utrecht (NL)
- Utrecht University (NL)
- University of California, San Francisco (US)
- Gladstone Institutes (US)
- Oncode Institute (NL)
- Princess Máxima Center (NL)
- ORCID
- Miao, Yi
- de Lau, Wim
- Yuki, Kanako
- Santos, António J.M.
- You, Changjiang
- Geurts, Maarten H.
- Puschhof, Jens
- Peng, Weng Chuan
- Buikema, Jan W.
- Gbenedio, Oghenekevwe M.
- Vallon, Mario
- Dang, Luke T.
- Baker, David
- Ott, Melanie
- Depeille, Philippe
- Wu, Sean M.
- Drost, Jarno
- Nusse, Roeland
- Roose, Jeroen P.
- Piehler, Jacob
- Boj, Sylvia F.
- Janda, Claudia Y.
- Clevers, Hans
- Kuo, Calvin J.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2020-11
- Volume
- 27
- Issue
- 5
- Pages
- 840-851.e6
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- PMC
- PMC7655723
- OpenAlex
- W3061588182
- Cited by
- 151
- References
- 57
- Citations by year
- 2026: 15 · 2025: 25 · 2024: 24 · 2023: 32 · 2022: 24 · 2021: 28 · 2020: 3
- FWCI
- 7.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell stem cell (publishedVersion)
- PubMed (publishedVersion)
- KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- HAL (submittedVersion, other-oa)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Ludwig Institute for Cancer Research
- G. Harold and Leila Y. Mathers Foundation
- Mark Foundation For Cancer Research
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- Wnt signaling, Frizzled receptors, LRP6, organoid expansion, liver zonation, stem cell homeostasis, regenerative medicine, kidney organoids, colon organoids, hepatocyte organoids
- MeSH terms
- Organoids, Stem Cells, Hepatocytes, Frizzled Receptors, Wnt Signaling Pathway
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