The DOI matches its Crossref record; 56 of the 57 references it cites were found in a registry or an index.
Acquisition of Dynamic Function in Human Stem Cell-Derived β Cells
Open accessAbstract
Recent advances in human pluripotent stem cell (hPSC) differentiation protocols have generated insulin-producing cells resembling pancreatic β cells. While these stem cell-derived β (SC-β) cells are capable of undergoing glucose-stimulated insulin secretion (GSIS), insulin secretion per cell remains low compared with islets and cells lack dynamic insulin release. Herein, we report a differentiation strategy focused on modulating transforming growth factor β (TGF-β) signaling, controlling cellular cluster size, and using an enriched serum-free media to generate SC-β cells that express β cell markers and undergo GSIS with first- and second-phase dynamic insulin secretion. Transplantation of these cells into mice greatly improves glucose tolerance. These results reveal that specific time frames for inhibiting and permitting TGF-β signaling are required during SC-β cell differentiation to achieve dynamic function. The capacity of these cells to undergo GSIS with dynamic insulin release makes them a promising cell source for diabetes cellular therapy.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Transplantation of SC-islets into mice improves their insulin secretion and glucose responsiveness, suggesting that SC-islet immaturity can be resolved.”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [42]
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“These cells have been shown to improve glycemic control in diabetic mice, highlighting their potential utility in vivo.36,37,39–44”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [42]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Zhang, Q., Wang, C., Zheng, M., Li, Y., Li, J., Zhang, L., Shang, X., & Yan, C. (2014) · Cerebrovascular Diseases · doi:10.1159/000369778 · Cited by 11,609
Projections of Global Mortality and Burden of Disease from 2002 to 2030
Mathers, C. D., & Loncar, D. (2006) · PLoS Medicine · doi:10.1371/journal.pmed.0030442 · Cited by 11,386
Shapiro, A. M. J., Lakey, J. R. T., Ryan, E. A., Korbutt, G. S., Toth, E., Warnock, G. L., Kneteman, N. M., & Rajotte, R. V. (2000) · New England Journal of Medicine · doi:10.1056/nejm200007273430401 · Cited by 5,237
Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas
Lacy, P. E., & Kostianovsky, M. (1967) · Diabetes · doi:10.2337/diab.16.1.35 · Cited by 2,741
Generation of Functional Human Pancreatic β Cells In Vitro
Pagliuca, F. W., Millman, J. R., Gürtler, M., Segel, M., Van Dervort, A., Ryu, J. H., Peterson, Q. P., Greiner, D., & Melton, D. A. (2014) · Cell · doi:10.1016/j.cell.2014.09.040 · Cited by 2,025
Self-citationInternational trial of the Edmonton protocol for islet transplantation
Shapiro, A. M. J., Ricordi, C., Hering, B. J., Auchincloss, H., Lindblad, R., Robertson, R. P., Secchi, A., Brendel, M. D., Berney, T., Brennan, D. C., et al. (2006) · New England Journal of Medicine · doi:10.1056/nejmoa061267 · Cited by 1,942
Production of pancreatic hormone–expressing endocrine cells from human embryonic stem cells
D’Amour, K. A., Bang, A. G., Eliazer, S., Kelly, O., Agulnick, A. D., Smart, N. G., Moorman, M. A., KROON, E. J., Carpenter, M. K., & Baetge, E. E. (2006) · Nature Biotechnology · doi:10.1038/nbt1259 · Cited by 1,913
Kroon, E., Martinson, L. A., Kadoya, K., Bang, A. G., Kelly, O. G., Eliazer, S., Young, H., Richardson, M., Smart, N. G., Cunningham, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1393 · Cited by 1,843 · cited 2×
“Through modulation of pathways identified from embryonic development, studies with hPSCs have detailed protocols for generating cells that resemble early endoderm and pancreatic progenitors (D’Amour et al., 2006; D’Amour et al., 2005; Kroon et al., 2008; Nostro et al., 2015; Rezania et al., 2012), the latter of which…”
BackgroundEfficient differentiation of human embryonic stem cells to definitive endoderm
D'Amour, K. A., Agulnick, A. D., Eliazer, S., Kelly, O. G., Kroon, E., & Baetge, E. E. (2005) · Nature Biotechnology · doi:10.1038/nbt1163 · Cited by 1,730
Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells
Rezania, A., Bruin, J. E., Arora, P., Rubin, A., Batushansky, I., Asadi, A., O'Dwyer, S., Quiskamp, N., Mojibian, M., Albrecht, T., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.3033 · Cited by 1,575
Flagged first, then by citations
1 of the 57 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Single‐cell RNA sequencing technologies and applications: A brief overview
Jovic, D., Liang, X., Zeng, H., Lin, L., Xu, F., & Luo, Y. (2022) · Clinical and Translational Medicine · doi:10.1002/ctm2.694 · Cited by 1,379
Balboa, D., Barsby, T., Lithovius, V., Saarimäki-Vire, J., Omar-Hmeadi, M., Dyachok, O., Montaser, H., Lund, P.-E., Yang, M., Ibrahim, H., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01219-z · Cited by 433
Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
Hogrebe, N. J., Augsornworawat, P., Maxwell, K. G., Velazco-Cruz, L., & Millman, J. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0430-6 · Cited by 373
Self-citationGeneration of insulin-producing pancreatic β cells from multiple human stem cell lines
Hogrebe, N. J., Maxwell, K. G., Augsornworawat, P., & Millman, J. R. (2021) · Nature Protocols · doi:10.1038/s41596-021-00560-y · Cited by 211
Self-citationDevelopments in stem cell-derived islet replacement therapy for treating type 1 diabetes
Hogrebe, N. J., Ishahak, M., & Millman, J. R. (2023) · Cell Stem Cell · doi:10.1016/j.stem.2023.04.002 · Cited by 210
Self-citationAugsornworawat, P., Maxwell, K. G., Velazco-Cruz, L., & Millman, J. R. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108067 · Cited by 200
Self-citationChiou, J., Zeng, C., Zhang, C., Han, J. Y., Schlichting, M., Miller, M., Mendez, R., Huang, S., Wang, J., Sui, Y., et al. (2021) · Nature Genetics · doi:10.1038/s41588-021-00823-0 · Cited by 186
Maxwell, K. G., Augsornworawat, P., Velazco-Cruz, L., Kim, M. H., Asada, R., Hogrebe, N. J., Morikawa, S., Urano, F., & Millman, J. R. (2020) · Science Translational Medicine · doi:10.1126/scitranslmed.aax9106 · Cited by 183
Self-citationDiagnosis and treatment of type 1 diabetes at the dawn of the personalized medicine era
Akil, A. A., Yassin, E., Al-Maraghi, A., ALIYEV, E. E., Al-Malki, K., & Fakhro, K. A. (2021) · Journal of Translational Medicine · doi:10.1186/s12967-021-02778-6 · Cited by 215
Mapping cells through time and space with moscot
Klein, D., Palla, G., Lange, M., Klein, M., Piran, Z., Gander, M., Meng-Papaxanthos, L., Sterr, M., Saber, L., Jing, C., et al. (2025) · Nature · doi:10.1038/s41586-024-08453-2 · Cited by 235
Emerging routes to the generation of functional β-cells for diabetes mellitus cell therapy
Nair, G. G., Tzanakakis, E. S., & Hebrok, M. (2020) · Nature Reviews Endocrinology · doi:10.1038/s41574-020-0375-3 · Cited by 134
Stem Cell-Based Clinical Trials for Diabetes Mellitus
de Klerk, E., & Hebrok, M. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.631463 · Cited by 111
Applications of iPSC-derived beta cells from patients with diabetes
Maxwell, K. G., & Millman, J. R. (2021) · Cell Reports Medicine · doi:10.1016/j.xcrm.2021.100238 · Cited by 146
Self-citationGlucose Response by Stem Cell-Derived β Cells In Vitro Is Inhibited by a Bottleneck in Glycolysis
Davis, J. C., Alves, T., Helman, A., Chen, J. C., Kenty, J. H. R., Cardone, R., Liu, D. R., Kibbey, R., & Melton, D. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107623 · Cited by 105
Insights into pancreatic islet cell dysfunction from type 2 diabetes mellitus genetics
Krentz, N. A. J., & Gloyn, A. L. (2020) · Nature Reviews Endocrinology · doi:10.1038/s41574-020-0325-0 · Cited by 129
Engineering islets from stem cells for advanced therapies of diabetes
Siehler, J., Blöchinger, A. K., Meier, M., & Lickert, H. (2021) · Nature Reviews Drug Discovery · doi:10.1038/s41573-021-00262-w · Cited by 125
Latres, E., Finan, D. A., Greenstein, J. L., Kowalski, A. J., & Kieffer, T. J. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.02.007 · Cited by 124
Wang, X., Maxwell, K. G., Wang, K., Bowers, D. T., Flanders, J. A., Liu, W., Wang, L.-H., Liu, Q., Liu, C., Naji, A., et al. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.abb4601 · Cited by 119
Self-citationAdvances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes
Cayabyab, F., Nih, L. R., & Yoshihara, E. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.732431 · Cited by 123
Zhan, L., Rao, J. S., Sethia, N., Slama, M. Q., Han, Z., Tobolt, D., Etheridge, M., Peterson, Q. P., Dutcher, C. S., Bischof, J. C., et al. (2022) · Nature Medicine · doi:10.1038/s41591-022-01718-1 · Cited by 115
Generation of pancreatic β cells from CD177+ anterior definitive endoderm
Mahaddalkar, P. U., Scheibner, K., Pfluger, S., Ansarullah, Sterr, M., Beckenbauer, J., Irmler, M., Beckers, J., Knöbel, S., & Lickert, H. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0492-5 · Cited by 115
Parent, A. V., Faleo, G., Chavez, J., Saxton, M., Berrios, D. I., Kerper, N. R., Tang, Q., & Hebrok, M. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109538 · Cited by 96
Primary cilia control glucose homeostasis via islet paracrine interactions
Hughes, J. W., Cho, J. H., Conway, H., DiGruccio, M. R., Ng, X. W., Roseman, H. F., Abreu, D., Urano, F., & Piston, D. W. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2001936117 · Cited by 90
Cell maturation: Hallmarks, triggers, and manipulation
Alvarez‐Dominguez, J. R., & Melton, D. A. (2022) · Cell · doi:10.1016/j.cell.2021.12.012 · Cited by 96
Zbinden, A., Marzi, J., Schlünder, K., Probst, C., Urbanczyk, M., Black, S., Brauchle, E., Layland, S. L., Kraushaar, U., Duffy, G. P., et al. (2019) · Matrix Biology · doi:10.1016/j.matbio.2019.06.008 · Cited by 92
Grattoni, A., Korbutt, G., Tomei, A. A., García, A. J., Pepper, A. R., Stabler, C., Brehm, M., Papas, K., Citro, A., Shirwan, H., et al. (2025) · Nature Reviews Endocrinology · doi:10.1038/s41574-024-01029-0 · Cited by 92
Self-citationAugsornworawat, P., Hogrebe, N. J., Ishahak, M., Schmidt, M. D., Marquez, E., Maestas, M. M., Veronese-Paniagua, D., Gale, S. E., Miller, J. R., Velazco-Cruz, L., et al. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01150-8 · Cited by 89
Self-citationβ-Cell Maturation and Identity in Health and Disease
Salinno, C., Cota, P., Bastidas-Ponce, A., Tarquis-Medina, M., Lickert, H., & Bakhti, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20215417 · Cited by 88
Silva, I. B. B., Kimura, C. H., Colantoni, V. P., & Sogayar, M. C. (2022) · Stem Cell Research & Therapy · doi:10.1186/s13287-022-02977-y · Cited by 99
Zhu, H., Wang, G., Nguyen-Ngoc, K.-V., Kim, D., Miller, M., Goss, G., Kovsky, J., Harrington, A. R., Saunders, D. C., Hopkirk, A. L., et al. (2023) · Developmental Cell · doi:10.1016/j.devcel.2023.03.011 · Cited by 88
Towards a better understanding of diabetes mellitus using organoid models
Beydag-Tasöz, B. S., Yennek, S., & Grapin‐Botton, A. (2023) · Nature Reviews Endocrinology · doi:10.1038/s41574-022-00797-x · Cited by 91
The Human Islet: Mini-Organ With Mega-Impact
Walker, J. T., Saunders, D. C., Briššová, M., & Powers, A. C. (2021) · Endocrine Reviews · doi:10.1210/endrev/bnab010 · Cited by 88
Deciphering early human pancreas development at the single-cell level
Ma, Z., Zhang, X., Zhong, W., Yi, H., Chen, X., Zhao, Y., Ma, Y., Song, E., & Xu, T. (2023) · Nature Communications · doi:10.1038/s41467-023-40893-8 · Cited by 85
Type 1 Diabetes Mellitus: A Review on Advances and Challenges in Creating Insulin Producing Devices
Oliveira, S. M. R., Rebocho, A., Ahmadpour, E., Nissapatorn, V., & Pereira, M. D. L. (2023) · Micromachines · doi:10.3390/mi14010151 · Cited by 92
Towards a Functional Cure for Diabetes Using Stem Cell-Derived Beta Cells: Are We There Yet?
Bourgeois, S., Sawatani, T., Mulders, A. V., Leu, N. D., Heremans, Y., Heimberg, H., Cnop, M., & Staels, W. (2021) · Cells · doi:10.3390/cells10010191 · Cited by 73
Fattahi, P., Rahimian, A., Slama, M. Q., Gwon, K., Gonzalez‐Suarez, A. M., Wolf, J., Baskaran, H., Duffy, C. D., Stybayeva, G., Peterson, Q. P., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-85786-2 · Cited by 69
ENTPD3 Marks Mature Stem Cell–Derived β-Cells Formed by Self-Aggregation In Vitro
Docherty, F. M., Riemondy, K. A., Castro-Gutiérrez, R., Dwulet, J. M., Shilleh, A. H., Hansen, M. S., Williams, S. P. M., Armitage, L. H., Santostefano, K. E., Wallet, M. A., et al. (2021) · Diabetes · doi:10.2337/db20-0873 · Cited by 70
Recent advances in the applications of iPSC technology
Wiegand, C., & Banerjee, I. (2019) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2019.05.011 · Cited by 69
Goswami, D., Domingo‐Lopez, D. A., Ward, N. A., Millman, J. R., Duffy, G. P., Dolan, E. B., & Roche, E. T. (2021) · Advanced Science · doi:10.1002/advs.202100820 · Cited by 72
Self-citationAdvances Toward Engineering Functionally Mature Human Pluripotent Stem Cell-Derived β Cells
Velazco-Cruz, L., Goedegebuure, M. M., & Millman, J. R. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00786 · Cited by 68
Self-citationWeng, C., Xi, J., Li, H., Cui, J., Gu, A., Lai, S., Leskov, K., Ke, L., Jin, F., & Li, Y. (2020) · Nature Metabolism · doi:10.1038/s42255-020-00314-2 · Cited by 65
Maturation of beta cells: lessons from in vivo and in vitro models
Barsby, T., & Otonkoski, T. (2022) · Diabetologia · doi:10.1007/s00125-022-05672-y · Cited by 68
Aghazadeh, Y., Sarangi, F., Poon, F., Nkennor, B., McGaugh, E. C., Nunes, S. S., & Nostro, M. C. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.03.004 · Cited by 62
Protecting Stem Cell Derived Pancreatic Beta-Like Cells From Diabetogenic T Cell Recognition
Castro-Gutiérrez, R., Alkanani, A. K., Mathews, C. E., Michels, A. W., & Russ, H. A. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.707881 · Cited by 60
Human pluripotent stem cell-derived insulin-producing cells: A regenerative medicine perspective
Migliorini, A., Nostro, M. C., & Sneddon, J. B. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2021.03.021 · Cited by 62
Loss of the transcription factor MAFB limits β-cell derivation from human PSCs
Russell, R., Carnese, P., Hennings, T. G., Walker, E. M., Russ, H. A., Liu, J. S., Giacometti, S., Stein, R. W., & Hebrok, M. (2020) · Nature Communications · doi:10.1038/s41467-020-16550-9 · Cited by 62
Differentiating functional human islet-like aggregates from pluripotent stem cells
Barsby, T., Ibrahim, H., Lithovius, V., Montaser, H., Balboa, D., Vähäkangas, E., Chandra, V., Saarimäki‐Vire, J., & Otonkoski, T. (2022) · STAR Protocols · doi:10.1016/j.xpro.2022.101711 · Cited by 58
Differential Function and Maturation of Human Stem Cell-Derived Islets After Transplantation
Maxwell, K. G., Kim, M. H., Gale, S. E., & Millman, J. R. (2022) · Stem Cells Translational Medicine · doi:10.1093/stcltm/szab013 · Cited by 54
Self-citationPancreatic islet transplantation: current advances and challenges
Wang, Q., Huang, Y., Liu, L., Zhao, X., Sun, Y., Mao, X., & Li, S.-W. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1391504 · Cited by 63
Transcriptional mechanisms of pancreatic β-cell maturation and functional adaptation
Wortham, M., & Sander, M. (2021) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2021.04.011 · Cited by 56
Human expandable pancreatic progenitor–derived β cells ameliorate diabetes
Ma, X., Lu, Y., Zhou, Z., Li, Q., Chen, X., Wang, W., Jin, Y., Hu, Z., Chen, G., Deng, Q., et al. (2022) · Science Advances · doi:10.1126/sciadv.abk1826 · Cited by 60
Islet organoid as a promising model for diabetes
Zhang, X., Ma, Z., Song, E., & Xu, T. (2021) · Protein & Cell · doi:10.1007/s13238-021-00831-0 · Cited by 58
Emerging diabetes therapies: Bringing back the β-cells
Basile, G., Qadir, M. M. F., Mauvais‐Jarvis, F., Vetere, A., Shoba, V. M., Modell, A. E., Pastori, R. L., Russ, H. A., Wagner, B. K., & Domínguez‐Bendala, J. (2022) · Molecular Metabolism · doi:10.1016/j.molmet.2022.101477 · Cited by 56
A Stem Cell Approach to Cure Type 1 DiabetesPreprint
Helman, A., & Melton, D. A. (2020) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a035741 · Cited by 56
Youngblood, R. L., Sampson, J. P., Lebioda, K. R., Spicer, G., & Shea, L. D. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.06.032 · Cited by 57
Augsornworawat, P., Velazco-Cruz, L., Song, J., & Millman, J. R. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.08.031 · Cited by 51
Self-citationAdvances in Cell Replacement Therapies for Diabetes
Hering, B. J., Rickels, M. R., Bellin, M. D., Millman, J. R., Tomei, A. A., Garcı́a, A. J., Shirwan, H., Stabler, C. L., Ma, M., Peng, Y., et al. (2025) · Diabetes · doi:10.2337/db25-0037 · Cited by 55
Self-citationCurrent status of stem cell therapy for type 1 diabetes: a critique and a prospective consideration
Ghoneim, M. A., Gabr, M. M., El-Halawani, S. M., & Refaie, A. F. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03636-0 · Cited by 49
Chmielowiec, J., Szlachcic, W. J., Yang, D., Scavuzzo, M. A., Wamble, K., Sarrion-Perdigones, A., Sabek, O., Venken, K., & Borowiak, M. (2022) · Nature Communications · doi:10.1038/s41467-022-29646-1 · Cited by 43
Yung, T. M., Poon, F., Liang, M., Coquenlorge, S., McGaugh, E. C., Hui, C., Wilson, M. D., Nostro, M. C., & Kim, T.-H. (2019) · Nature Communications · doi:10.1038/s41467-019-12624-5 · Cited by 45
Pancreas-on-a-Chip Technology for Transplantation Applications
Abadpour, S., Aizenshtadt, A., Olsen, P. A., Shoji, K., Wilson, S. R., Krauss, S. J. K., & Scholz, H. (2020) · Current Diabetes Reports · doi:10.1007/s11892-020-01357-1 · Cited by 52
Reduced replication fork speed promotes pancreatic endocrine differentiation and controls graft size
Sui, L., Xin, Y., Du, Q., Georgieva, D., Diedenhofen, G., Haataja, L., Su, Q., Zuccaro, M. V., Kim, J., Fu, J., et al. (2021) · JCI Insight · doi:10.1172/jci.insight.141553 · Cited by 47
Blanchi, B., Taurand, M., Sauty-Colace, C., Thomaidou, S., Audeoud, C., Fantuzzi, F., Sawatani, T., Gheibi, S., Sabadell‐Basallote, J., Boot, F., et al. (2023) · Molecular Metabolism · doi:10.1016/j.molmet.2023.101772 · Cited by 55
Identification of unique cell type responses in pancreatic islets to stress
Maestas, M. M., Ishahak, M., Augsornworawat, P., Veronese-Paniagua, D., Maxwell, K. G., Velazco-Cruz, L., Marquez, E., Sun, J., Shunkarova, M., Gale, S. E., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49724-w · Cited by 53
Self-citationHuman Pluripotent Stem Cells to Model Islet Defects in Diabetes
Balboa, D., Iworima, D. G., & Kieffer, T. J. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.642152 · Cited by 49
Fantuzzi, F., Toivonen, S., Schiavo, A. A., Chae, H., Mohammad, T., Sawatani, T., Pachera, N., Cai, Y., Vinci, C., Virgilio, E., et al. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.967765 · Cited by 42
Cell therapy for type 1 diabetes
Loretelli, C., Assi, E., Seelam, A. J., Nasr, M. B., & Fiorina, P. (2020) · Expert Opinion on Biological Therapy · doi:10.1080/14712598.2020.1748596 · Cited by 47
SIX2 Regulates Human β Cell Differentiation from Stem Cells and Functional Maturation In Vitro
Velazco-Cruz, L., Goedegebuure, M. M., Maxwell, K. G., Augsornworawat, P., Hogrebe, N. J., & Millman, J. R. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107687 · Cited by 45
Self-citationBatra, L., Shrestha, P., Zhao, H., Woodward, K. B., Togay, A., Tan, M., Grimany-Nuno, O., Malik, M. T., Coronel, M. M., Garcı́a, A. J., et al. (2020) · The Journal of Immunology · doi:10.4049/jimmunol.2000055 · Cited by 42
Emerging strategies for beta cell transplantation to treat diabetes
Paez‐Mayorga, J., Lukin, I., Emerich, D. F., de Vos, P., Orive, G., & Grattoni, A. (2021) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2021.11.007 · Cited by 43
Characterization of stem-cell-derived islets during differentiation and after implantation
Cuesta-Gomez, N., Verhoeff, K., Jasra, I. T., Pawlick, R., Dadheech, N., & Shapiro, A. M. J. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.111238 · Cited by 41
Immune Protection of Stem Cell-Derived Islet Cell Therapy for Treating Diabetes
Tahbaz, M., & Yoshihara, E. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.716625 · Cited by 42
Type 1 diabetes and engineering enhanced islet transplantation
Jeyagaran, A., Lu, C., Zbinden, A., Birkenfeld, A. L., Brucker, S. Y., & Layland, S. L. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114481 · Cited by 42
Strategies to Improve the Safety of iPSC-Derived β Cells for β Cell Replacement in Diabetes
Pellegrini, S., Zamarian, V., & Sordi, V. (2022) · Transplant International · doi:10.3389/ti.2022.10575 · Cited by 38
Barra, J. M., Robino, R. A., Castro-Gutiérrez, R., Proia, J., Russ, H. A., & Ferreira, L. M. R. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114994 · Cited by 40
Modeling different types of diabetes using human pluripotent stem cells
Abdelalim, E. M. (2020) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03710-9 · Cited by 38
Mimicking nature-made beta cells: recent advances towards stem cell-derived islets
Tremmel, D. M., Mitchell, S. A., Sackett, S. D., & Odorico, J. S. (2019) · Current Opinion in Organ Transplantation · doi:10.1097/mot.0000000000000687 · Cited by 37
Deciphering the Complex Communication Networks That Orchestrate Pancreatic Islet Function
Weitz, J., Menegaz, D., & Caicedo, A. (2020) · Diabetes · doi:10.2337/dbi19-0033 · Cited by 39
Development of a scalable method to isolate subsets of stem cell-derived pancreatic islet cells
Parent, A. V., Ashe, S., Nair, G. G., Li, M.-L., Chavez, J., Liu, J. S., Zhong, Y., Streeter, P. R., & Hebrok, M. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.02.001 · Cited by 37
Iworima, D. G., Baker, R. K., Ellis, C. E., Sherwood, C., Zhan, L., Rezania, A., Piret, J. M., & Kieffer, T. J. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-023-03574-3 · Cited by 36
Basile, G., Kahraman, S., Dirice, E., Pan, H., Dreyfuss, J. M., & Kulkarni, R. N. (2021) · Genome Medicine · doi:10.1186/s13073-021-00941-8 · Cited by 33
Krentz, N. A. J., Shea, L. D., Huising, M. O., & Shaw, J. (2021) · The Lancet Diabetes & Endocrinology · doi:10.1016/s2213-8587(21)00170-4 · Cited by 39
A Century-long Journey From the Discovery of Insulin to the Implantation of Stem Cell–derived Islets
Ramzy, A., Belmonte, P. J., Braam, M. J. S., Ida, S., Wilts, E. M., Levings, M. K., Rezania, A., & Kieffer, T. J. (2022) · Endocrine Reviews · doi:10.1210/endrev/bnac021 · Cited by 37
Pancreatic β-Cell Development and RegenerationPreprint
Kerper, N. R., Ashe, S., & Hebrok, M. (2021) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a040741 · Cited by 38
SUR1-mutant iPS cell-derived islets recapitulate the pathophysiology of congenital hyperinsulinism
Lithovius, V., Saarimäki-Vire, J., Balboa, D., Ibrahim, H., Montaser, H., Barsby, T., & Otonkoski, T. (2021) · Diabetologia · doi:10.1007/s00125-020-05346-7 · Cited by 34
Genetic Engineering of Immune Evasive Stem Cell-Derived Islets
Sackett, S. D., Kaplan, S. J., Mitchell, S. A., Brown, M. E., Burrack, A. L., Grey, S., Huangfu, D., & Odorico, J. (2022) · Transplant International · doi:10.3389/ti.2022.10817 · Cited by 32
Pancreatic β-cell heterogeneity in adult human islets and stem cell-derived islets
Aldous, N., Moin, A. S. M., & Abdelalim, E. M. (2023) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-023-04815-7 · Cited by 40
Pancreatic β-cell failure, clinical implications, and therapeutic strategies in type 2 diabetes
Cui, D., Feng, X., Lei, S., Zhang, H., Hu, W., Yang, S., Yu, X., & Su, Z. (2024) · Chinese Medical Journal · doi:10.1097/cm9.0000000000003034 · Cited by 40
Jun, Y., Nguyen-Ngoc, K.-V., Sai, S., Bender, R. H. F., Gong, W., Kravets, V. V., Zhu, H., Hatch, C. J., Schlichting, M., Gaetani, R., et al. (2022) · Developmental Cell · doi:10.1016/j.devcel.2025.04.024 · Cited by 43
Kim, M. J., Lee, E. Y., You, Y., Yang, H. K., Yoon, K., & Kim, J.-W. (2020) · Stem Cell Research · doi:10.1016/j.scr.2020.101958 · Cited by 34
Li, H., Zhu, H., Ge, T., Wang, Z., & Zhang, C. (2021) · Stem Cell Reviews and Reports · doi:10.1007/s12015-021-10139-5 · Cited by 36
Andersson-Rolf, A., Groot, K., Korving, J., Begthel, H., Hanegraaf, M. A. J., VanInsberghe, M. A., Salmén, F., van den Brink, S., López‐Iglesias, C., Peters, P. J., et al. (2024) · Cell · doi:10.1016/j.cell.2024.10.044 · Cited by 35
Stem Cells, Self-Renewal, and Lineage Commitment in the Endocrine System
Mariniello, K., Ruiz‐Babot, G., McGaugh, E. C., Nicholson, J. G., Gualtieri, A., Gaston‐Massuet, C., Nostro, M. C., & Guasti, L. (2019) · Frontiers in Endocrinology · doi:10.3389/fendo.2019.00772 · Cited by 33
The hepatokine fetuin-A disrupts functional maturation of pancreatic beta cells
Gerst, F., Kemter, E., Lorza‐Gil, E., Kaiser, G., Fritz, A.-K., Nano, R., Piemonti, L., Gauder, M., Dahl, A., Nadalin, S., et al. (2021) · Diabetologia · doi:10.1007/s00125-021-05435-1 · Cited by 31
Generation of Pancreatic Progenitors from Human Pluripotent Stem Cells by Small Molecules
Jiang, Y., Chen, C., Randolph, L. N., Ye, S., Zhang, X., Bao, X., & Lian, X. L. (2020) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.07.021 · Cited by 32
Pignatelli, C., Campo, F., Neroni, A., Piemonti, L., & Citro, A. (2022) · Transplant International · doi:10.3389/ti.2022.10555 · Cited by 33
Schmidt, M. D., Ishahak, M., Augsornworawat, P., & Millman, J. R. (2024) · BMC Genomics · doi:10.1186/s12864-024-10013-x · Cited by 28
Self-citationEncapsulation and immune protection for type 1 diabetes cell therapy
Kioulaphides, S., & Garcı́a, A. J. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115205 · Cited by 37
Genome editing of human pancreatic beta cell models: problems, possibilities and outlook
Balboa, D., Prasad, R. B., Groop, L., & Otonkoski, T. (2019) · Diabetologia · doi:10.1007/s00125-019-4908-z · Cited by 31
Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets
Kim, M., Cho, S., Hwang, D. G., Shim, I. K., Kim, S. C., Jang, J., & Jang, J. (2025) · Nature Communications · doi:10.1038/s41467-025-56665-5 · Cited by 32
Metadata
- Authors
- Velazco-Cruz, L., Song, J., Maxwell, K. G., Goedegebuure, M. M., Augsornworawat, P., Hogrebe, N. J., & Millman, J. R.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Millman, Jeffrey R.
- ORCID
- Venue
- Stem Cell Reports
- Publisher
- Elsevier BV
- Published
- 2019-02
- Volume
- 12
- Issue
- 2
- Pages
- 351-365
- ISSN
- 2213-6711 (ISSN-L)
- PMC
- PMC6372986
- OpenAlex
- W2910245538
- Cited by
- 399
- References
- 57
- Citations by year
- 2026: 45 · 2025: 49 · 2024: 54 · 2023: 56 · 2022: 61 · 2021: 72 · 2020: 42 · 2019: 20
- FWCI
- 46.82
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cell Reports (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Digital Commons@Becker (Washington University School of Medicine) (submittedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- NIH (5R01DK114233, 5P30DK020579, 2R25GM103757, 5T32DK108742, 5T32DK007120)
- JDRF (5-CDA-2017-391-A-N)
- Washington University Center of Regenerative Medicine
- Washington University School of Medicine
- Hope Center Viral Vectors Core
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- stem cell-derived beta cells, human pluripotent stem cells, glucose-stimulated insulin secretion, TGF-beta signaling, beta cell differentiation, serum-free medium, islet transplantation, glucose tolerance, diabetes cell therapy
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, Glucose, Humans, Insulin, Pancreas, Signal Transduction, Transforming Growth Factor beta, Pluripotent Stem Cells, Insulin-Secreting Cells, Mice, Embryonic Stem Cells, Cell- and Tissue-Based Therapy
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
