Cited by 860Metabolism, Diabetes, and CancerBiochemical and Molecular ResearchPI3K/AKT/mTOR signaling in cancer

The DOI matches its Crossref record; 75 of the 75 references it cites were found in a registry or an index.

AMPK: An Energy-Sensing Pathway with Multiple Inputs and Outputs

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Hardie, D. Grahame, Schaffer, Bethany E., Brunet, Anne·2016·Trends in Cell Biology

Abstract

AMP-activated protein kinase (AMPK) is a key regulator of energy balance expressed ubiquitously in eukaryotic cells. Here we review the canonical adenine nucleotide-dependent mechanism that activates AMPK when cellular energy status is compromised, as well as other, noncanonical activation mechanisms. Once activated, AMPK acts to restore energy homeostasis by promoting catabolic pathways, resulting in ATP generation, and inhibiting anabolic pathways that consume ATP. We also review the various hypothesis-driven and unbiased approaches that have been used to identify AMPK substrates and have revealed substrates involved in both metabolic and non-metabolic processes. We particularly focus on methods for identifying the AMPK target recognition motif and how it can be used to predict new substrates.

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Metadata

Authors
Hardie, D. G., Schaffer, B. E., & Brunet, A.
Affiliations
  • University of Dundee (GB)
  • Stanford University (US)
Corresponding author
Hardie, D. Grahame
Venue
Trends in Cell Biology
Publisher
Elsevier BV
Published
2016-03
Volume
26
Issue
3
Pages
190-201
ISSN
0962-8924 (ISSN-L)
OpenAlex
W2174118934
Cited by
859
References
75
Citations by year
2026: 41 · 2025: 62 · 2024: 80 · 2023: 69 · 2022: 89 · 2021: 112 · 2020: 97 · 2019: 77 · 2018: 103 · 2017: 80 · 2016: 46
FWCI
7.43
Type
journal article
Language
en
Open access
Locations
  • Trends in Cell Biology (acceptedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • Discovery Research Portal (University of Dundee) (submittedVersion, cc-by-nc-nd)
Funders
  • Wellcome Trust (097726)
  • Cancer Research UK (C37030/A15101)
  • CIRM (RB4-06087)
  • NIH (R01 AG031198, T32 CA09302)
  • NSF GRFP
  • Robert M. and Anne T. Bass Stanford Graduate Fellowship
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
Keywords
AMPK, AMPK activation, energy homeostasis, catabolic pathways, ATP production, substrate recognition motif, substrate prediction
MeSH terms
Allosteric Regulation, Amino Acid Sequence, Animals, Energy Metabolism, Enzyme Activation, Humans, Phosphorylation, Protein Processing, Post-Translational, Signal Transduction, Catalytic Domain, AMP-Activated Protein Kinases

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